A novel functional human eukaryotic translation initiation factor 4G.

abstract：:Recombinant type 1 transforming growth factor beta (TGF-beta) was expressed to high levels in CHO cells by using dihydrofolate reductase (dhfr) gene amplification. The expression plasmid was derived from the pSV2 vectors and contained, in tandem, the simian TGF-beta and mouse dhfr cDNAs. Transcription of both cDNAs wa...

journal_title：Molecular and cellular biology

authors： Gentry LE,Webb NR,Lim GJ,Brunner AM,Ranchalis JE,Twardzik DR,Lioubin MN,Marquardt H,Purchio AF

更新日期：1987-10-01 00:00:00

abstract：:The homeo box gene even-skipped (eve) encodes a 376-amino-acid protein that binds with high affinity to sequences located near the 5' termini of the eve and en genes. The 5' en sites are A + T rich and contain copies of the 10-base-pair (bp) consensus sequence T-C-A-A-T-T-A-A-A-T. In contrast, the 5' eve sites are G +...

journal_title：Molecular and cellular biology

authors： Hoey T,Warrior R,Manak J,Levine M

更新日期：1988-11-01 00:00:00

abstract：:CPC1 is the transcriptional activator of amino acid biosynthetic genes of Neurospora crassa. CPC1 function in vivo was abolished upon deletion of segments of cpc-1 corresponding to the presumed transcription activation domain, the DNA-binding and dimerization domains, or a 52-residue connector segment of CPC1. A trunc...

journal_title：Molecular and cellular biology

authors： Paluh JL,Yanofsky C

更新日期：1991-02-01 00:00:00

abstract：:rRNA transcription in Xenopus laevis terminates near a 7-base-pair (bp) conserved sequence (T3 box) located 200 bp upstream of the site of transcription initiation for the adjacent gene promoter. We present evidence here that a 12-bp element containing the T3 box is an essential part of the terminator. Using an oocyte...

journal_title：Molecular and cellular biology

authors： Labhart P,Reeder RH

更新日期：1987-05-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a key angiogenic factor expressed under restricted nutrient and oxygen conditions in most solid tumors. The expression of VEGF under hypoxic conditions requires transcription through activated hypoxia-inducible factor 1 (HIF-1), increased mRNA stability, and facilitated tra...

journal_title：Molecular and cellular biology

authors： Vumbaca F,Phoenix KN,Rodriguez-Pinto D,Han DK,Claffey KP

更新日期：2008-01-01 00:00:00

abstract：:The first intron of the human collagen alpha 1(I) gene contains several positively and negatively acting elements. We have studied the transcription of collagen-human growth hormone fusion genes, containing deletions and rearrangements of collagen intronic sequences, by transient transfection of chick tendon fibroblas...

journal_title：Molecular and cellular biology

authors： Bornstein P,McKay J,Liska DJ,Apone S,Devarayalu S

更新日期：1988-11-01 00:00:00

abstract：:Following chromosome duplication in S phase of the cell cycle, the sister chromatids are linked by cohesin. At the onset of anaphase, separase cleaves cohesin and thereby initiates sister chromatid separation. Separase activation results from the destruction of its inhibitor, securin, which is triggered by a ubiquitin...

journal_title：Molecular and cellular biology

authors： Sullivan M,Holt L,Morgan DO

更新日期：2008-09-01 00:00:00

abstract：:Temperature-sensitive mutants of TFIIB that are defective for essential interactions were isolated. One mutation (G204D) results in disruption of a protein-protein contact between TFIIB and TATA binding protein (TBP), while the other (K272I) disrupts an interaction between TFIIB and DNA. The TBP gene was mutagenized, ...

journal_title：Molecular and cellular biology

authors： Buratowski RM,Downs J,Buratowski S

更新日期：2002-12-01 00:00:00

abstract：:The heterochromatic domains of Drosophila melanogaster (pericentric heterochromatin, telomeres, and the fourth chromosome) are characterized by histone hypoacetylation, high levels of histone H3 methylated on lysine 9 (H3-mK9), and association with heterochromatin protein 1 (HP1). While the specific interaction of HP1...

journal_title：Molecular and cellular biology

authors： Sun FL,Haynes K,Simpson CL,Lee SD,Collins L,Wuller J,Eissenberg JC,Elgin SC

更新日期：2004-09-01 00:00:00

abstract：:The minimal muscle-specific dystrophin promoter contains the consensus sequence CC(A/T)6GG, or the CArG element, which can be found in serum-inducible or muscle-specific promoters. The serum response factor (SRF), which mediates the transcriptional activation of the c-fos gene in response to serum stimulation, can bin...

journal_title：Molecular and cellular biology

authors： Galvagni F,Lestingi M,Cartocci E,Oliviero S

更新日期：1997-03-01 00:00:00

abstract：:The role of chromatin-remodeling factors in transcription is well established, but the link between chromatin-remodeling complexes and DNA repair remains unexplored. Human Rvb1 and Rvb2 are highly conserved AAA(+) ATP binding proteins that are part of various chromatin-remodeling complexes, such as Ino80, SNF2-related...

journal_title：Molecular and cellular biology

authors： Jha S,Shibata E,Dutta A

更新日期：2008-04-01 00:00:00

abstract：:Runx1/AML1 is a transcription factor implicated in tissue stem cell regulation and belongs to the small Runx family of cancer genes. In the hair follicle (HF), Runx1 epithelial deletion in morphogenesis impairs normal adult hair homeostasis (cycle) and blocks adult hair follicle stem cells (HFSCs) in quiescence. Here,...

journal_title：Molecular and cellular biology

authors： Hoi CS,Lee SE,Lu SY,McDermitt DJ,Osorio KM,Piskun CM,Peters RM,Paus R,Tumbar T

更新日期：2010-05-01 00:00:00

abstract：:The Ki-67 protein is widely used as a tumor proliferation marker. However, whether Ki-67 affects cell cycle progression has been controversial. Here we demonstrate that depletion of Ki-67 in human hTERT-RPE1, WI-38, IMR90, and hTERT-BJ cell lines and primary fibroblast cells slowed entry into S phase and coordinately ...

journal_title：Molecular and cellular biology

authors： Sun X,Bizhanova A,Matheson TD,Yu J,Zhu LJ,Kaufman PD

更新日期：2017-08-11 00:00:00

abstract：:We have isolated and subcloned three separate segments of human DNA which share strong sequence homology with a previously sequenced gene encoding a type I keratin, K14 (50 kilodaltons). Restriction endonuclease mapping has demonstrated that these three genes are tightly linked chromosomally, whereas the K14 gene appe...

journal_title：Molecular and cellular biology

authors： RayChaudhury A,Marchuk D,Lindhurst M,Fuchs E

更新日期：1986-02-01 00:00:00

abstract：:The genes of the trithorax group (trxG) in Drosophila melanogaster are required to maintain the pattern of homeotic gene expression that is established early in embryogenesis by the transient expression of the segmentation genes. The precise role of each of the diverse trxG members and the functional relationships amo...

journal_title：Molecular and cellular biology

authors： Crosby MA,Miller C,Alon T,Watson KL,Verrijzer CP,Goldman-Levi R,Zak NB

更新日期：1999-02-01 00:00:00

abstract：:Mouse neuroblastoma Neuro-2A cells produce transforming growth factors during exponential growth in a defined hormone-free medium, which, on Bio-Gel columns in 1 M HAc, elute at a molecular size of 15 to 20 kilodaltons (kDa). These neuroblastoma-derived transforming growth factors have strong mitogenic activity, but t...

journal_title：Molecular and cellular biology

authors： van Zoelen EJ,van de Ven WJ,Franssen HJ,van Oostwaard TM,van der Saag PT,Heldin CH,de Laat SW

更新日期：1985-09-01 00:00:00

abstract：:The human genome contains a number of methyl CpG binding proteins that translate DNA methylation into a physiological response. To gain insight into the function of MBD2 and MBD3, we first applied protein tagging and mass spectrometry. We show that MBD2 and MBD3 assemble into mutually exclusive distinct Mi-2/NuRD-like...

journal_title：Molecular and cellular biology

authors： Le Guezennec X,Vermeulen M,Brinkman AB,Hoeijmakers WA,Cohen A,Lasonder E,Stunnenberg HG

更新日期：2006-02-01 00:00:00

abstract：:Sequencing studies have shown that in somatic cells alternating runs of purines and pyrimidines are frequently associated with recombination crossover points. To test whether such sequences actually promote recombination, we have examined the effects of poly[d(pGpT).d(pApC)] and poly[d(pCpG).d(pCpG)] repeats on a homo...

journal_title：Molecular and cellular biology

authors： Bullock P,Miller J,Botchan M

更新日期：1986-11-01 00:00:00

abstract：:Yeast metallothionein, encoded by the CUP1 gene, and its copper-dependent transcriptional activator ACE1 play a key role in mediating copper resistance in Saccharomyces cerevisiae. Using an ethyl methanesulfonate mutant of a yeast strain in which CUP1 and ACE1 were deleted, we isolated a gene, designated CUP9, which p...

journal_title：Molecular and cellular biology

authors： Knight SA,Tamai KT,Kosman DJ,Thiele DJ

更新日期：1994-12-01 00:00:00

abstract：:Recent research has identified critical roles for microRNAs in a large number of cellular processes, including tumorigenic transformation. While significant progress has been made towards understanding the mechanisms of gene regulation by microRNAs, much less is known about factors affecting the expression of these no...

journal_title：Molecular and cellular biology

authors： Kulshreshtha R,Ferracin M,Wojcik SE,Garzon R,Alder H,Agosto-Perez FJ,Davuluri R,Liu CG,Croce CM,Negrini M,Calin GA,Ivan M

更新日期：2007-03-01 00:00:00

abstract：:Members of the cytokine receptor superfamily have structurally similar extracellular ligand-binding domains yet diverse cytoplasmic regions lacking any obvious catalytic domains. Many of these receptors form ligand-induced oligomers which are likely to participate in transmembrane signaling. A constitutively active (f...

journal_title：Molecular and cellular biology

authors： Watowich SS,Hilton DJ,Lodish HF

更新日期：1994-06-01 00:00:00

abstract：:The ubiquitin-proteasome pathway (UPP) is involved in regulation of multiple cellular processes. Hypoxia-inducible factor 1 alpha (HIF-1 alpha) is a prototypic target of the UPP and, as such, is stabilized under conditions of proteasomal inhibition. Using carbonic anhydrase IX (CAIX) and vascular endothelial growth fa...

journal_title：Molecular and cellular biology

authors： Kaluz S,Kaluzová M,Stanbridge EJ

更新日期：2006-08-01 00:00:00

abstract：:ING1 is a type II tumor suppressor that affects cell growth, stress signaling, apoptosis, and DNA repair by altering chromatin structure and regulating transcription. Decreased ING1 expression is seen in several human cancers, and mislocalization has been noted in diverse types of cancer cells. Aberrant targeting may,...

journal_title：Molecular and cellular biology

authors： Gong W,Russell M,Suzuki K,Riabowol K

更新日期：2006-04-01 00:00:00

abstract：:Using a subtractive cloning scheme on cDNA prepared from primary pro-B and pre-B cells, we identified several genes whose products regulate apoptosis. We further characterized one of these genes, encoding protein kinase Ceta (PKCeta). PKCeta transcripts were readily detected in pro-B cells but were absent in pre-B cel...

journal_title：Molecular and cellular biology

authors： Morrow TA,Muljo SA,Zhang J,Hardwick JM,Schlissel MS

更新日期：1999-08-01 00:00:00

abstract：:A pool of Candida albicans RsaI fragments cloned onto a vector containing pBR322 sequences and the Candida ADE2 gene was used to transform a Candida ade2 mutant to adenine protrophy. A potential autonomously replicating sequence (ARS) in Candida DNA was identified by two criteria: instability of the selectable marker ...

journal_title：Molecular and cellular biology

authors： Kurtz MB,Cortelyou MW,Miller SM,Lai M,Kirsch DR

更新日期：1987-01-01 00:00:00

abstract：:The natriuretic peptide receptors are three homologous cell surface proteins, each with a single transmembrane domain. The atrial natriuretic peptide receptor type A (ANPRA) and the homologous receptor type B (ANPRB) are both membrane guanylyl cyclases that synthesize cyclic GMP as an intracellular second messenger. T...

journal_title：Molecular and cellular biology

authors： Wilcox JN,Augustine A,Goeddel DV,Lowe DG

更新日期：1991-07-01 00:00:00

abstract：:Multiple members of the A, B, and C clusters of Hox genes are expressed in hematopoietic cells. Several of these Hox genes have been found to display distinctive expression patterns, with genes located at the 3' side of the clusters being expressed at their highest levels in the most primitive subpopulation of human C...

journal_title：Molecular and cellular biology

authors： Thorsteinsdottir U,Sauvageau G,Hough MR,Dragowska W,Lansdorp PM,Lawrence HJ,Largman C,Humphries RK

更新日期：1997-01-01 00:00:00

abstract：:CD146, an endothelial biomarker, has been shown to be aberrantly upregulated during pathological angiogenesis and functions as a coreceptor for vascular endothelial growth factor receptor 2 (VEGFR-2) to promote disease progression. However, the regulatory mechanisms of CD146 expression during angiogenesis remain uncle...

journal_title：Molecular and cellular biology

authors： Wang P,Luo Y,Duan H,Xing S,Zhang J,Lu D,Feng J,Yang D,Song L,Yan X

更新日期：2013-09-01 00:00:00

abstract：:The Id subfamily of helix-loop-helix (HLH) proteins plays a fundamental role in the regulation of cellular proliferation and differentiation. The major mechanism by which Id proteins are thought to inhibit differentiation is through interaction with other HLH proteins and inhibition of their DNA-binding activity. Howe...

journal_title：Molecular and cellular biology

authors： Roberts EC,Deed RW,Inoue T,Norton JD,Sharrocks AD

更新日期：2001-01-01 00:00:00

abstract：:Using several actin isotype-specific cDNA probes, we found actin mRNA of two size classes, 2.1 and 1.5 kilobases (kb), in extracts of polyadenylated and nonpolyadenylated RNA from sexually mature CD-1 mouse testes. Although the 2.1-kb sequence was present in both meiotic and postmeiotic testicular cell types, it decre...

journal_title：Molecular and cellular biology

authors： Waters SH,Distel RJ,Hecht NB

更新日期：1985-07-01 00:00:00