A microRNA signature of hypoxia.

abstract：:Progesterone receptor (PR) and ErbB-2 bidirectional cross talk participates in breast cancer development. Here, we identified a new mechanism of the PR and ErbB-2 interaction involving the PR induction of ErbB-2 nuclear translocation and the assembly of a transcriptional complex in which ErbB-2 acts as a coactivator o...

journal_title：Molecular and cellular biology

authors： Béguelin W,Díaz Flaqué MC,Proietti CJ,Cayrol F,Rivas MA,Tkach M,Rosemblit C,Tocci JM,Charreau EH,Schillaci R,Elizalde PV

更新日期：2010-12-01 00:00:00

abstract：:Histone acetylation and deacetylation have been implicated in the regulation of gene expression. Molecular studies have shown that histone deacetylases (HDACs) function as transcriptional repressors. However, very little is known about their roles during development in multicellular organisms. We previously demonstrat...

journal_title：Molecular and cellular biology

authors： Dufourcq P,Victor M,Gay F,Calvo D,Hodgkin J,Shi Y

更新日期：2002-05-01 00:00:00

abstract：:Two signals are required for meiosis and spore formation in the yeast Saccharomyces cerevisiae: starvation and the MAT products a1 and alpha 2, which determine the a/alpha cell type. These signals lead to increased expression of the IME1 (inducer of meiosis) gene, which is required for sporulation and sporulation-spec...

journal_title：Molecular and cellular biology

authors： Smith HE,Su SS,Neigeborn L,Driscoll SE,Mitchell AP

更新日期：1990-12-01 00:00:00

abstract：:Glucose-6-phosphate dehydrogenase (G6PD) produces cellular NADPH, which is required for the biosynthesis of fatty acids and cholesterol. Although G6PD is required for lipogenesis, it is poorly understood whether G6PD in adipocytes is involved in energy homeostasis, such as lipid and glucose metabolism. We report here ...

journal_title：Molecular and cellular biology

authors： Park J,Rho HK,Kim KH,Choe SS,Lee YS,Kim JB

更新日期：2005-06-01 00:00:00

abstract：:Yeast metallothionein, encoded by the CUP1 gene, and its copper-dependent transcriptional activator ACE1 play a key role in mediating copper resistance in Saccharomyces cerevisiae. Using an ethyl methanesulfonate mutant of a yeast strain in which CUP1 and ACE1 were deleted, we isolated a gene, designated CUP9, which p...

journal_title：Molecular and cellular biology

authors： Knight SA,Tamai KT,Kosman DJ,Thiele DJ

更新日期：1994-12-01 00:00:00

abstract：:Mutant strain d48 of Paramecium tetraurelia lacks the gene for antigen A in the macronucleus, whereas this gene is present in the micronucleus. Transfer of macronucleoplasm from the wild type to strain d48 caused d48 to revert to the wild type after autogamy. Transfer of cytoplasm was not as effective as transfer of m...

journal_title：Molecular and cellular biology

authors： Harumoto T

更新日期：1986-10-01 00:00:00

abstract：:Degenerate oligonucleotide probes corresponding to a highly conserved region common to epidermal growth factor, transforming growth factor alpha, and vaccinia growth factor were used to identify a novel growth factor gene in the Shope fibroma virus genome. Sequence analysis indicates that the Shope fibroma growth fact...

journal_title：Molecular and cellular biology

authors： Chang W,Upton C,Hu SL,Purchio AF,McFadden G

更新日期：1987-01-01 00:00:00

abstract：:Entry into mitosis depends upon activation of the dual-specificity phosphatase Cdc25C, which dephosphorylates and activates the cyclin B-Cdc2 complex. Previous work has shown that the Xenopus polo-like kinase Plx1 can phosphorylate and activate Cdc25C in vitro. In the work presented here, we demonstrate that Plx1 is a...

journal_title：Molecular and cellular biology

authors： Qian YW,Erikson E,Li C,Maller JL

更新日期：1998-07-01 00:00:00

abstract：:The Reelin signaling cascade plays a crucial role in the correct positioning of neurons during embryonic brain development. Reelin binding to apolipoprotein E receptor 2 (ApoER2) and very-low-density-lipoprotein receptor (VLDLR) leads to phosphorylation of disabled 1 (Dab1), an adaptor protein which associates with th...

journal_title：Molecular and cellular biology

authors： Strasser V,Fasching D,Hauser C,Mayer H,Bock HH,Hiesberger T,Herz J,Weeber EJ,Sweatt JD,Pramatarova A,Howell B,Schneider WJ,Nimpf J

更新日期：2004-02-01 00:00:00

abstract：:Cloned segments of the mouse glycerol-3-phosphate dehydrogenase (GPDH) gene, Gdc-1, were used to screen a human library. Human clones obtained spanned 25 kilobases of genomic DNA containing the human GPDH gene, GPD1. The 4 kb of sequence obtained from the 5'-flanking region and first exon of GPD1 was compared with the...

journal_title：Molecular and cellular biology

authors： Gwynn B,Lyford KA,Birkenmeier EH

更新日期：1990-10-01 00:00:00

abstract：:Transforming growth factor-beta (TGF-beta) is a secreted polypeptide factor that is thought to play a major role in the regulation of proliferation of many cell types and various differentiation processes. Several related isoforms have been structurally characterized, three of which, TGF-beta 1, -beta 2, and -beta 3, ...

journal_title：Molecular and cellular biology

authors： Lopez AR,Cook J,Deininger PL,Derynck R

更新日期：1992-04-01 00:00:00

abstract：:The Epstein-Barr virus BRLF1 and BZLF1 genes are the first viral genes transcribed upon induction of the viral lytic cycle. The protein products of both genes (referred to here as Rta and Zta, respectively) activate expression of other viral genes, thereby initiating the lytic cascade. Among the viral antigens express...

journal_title：Molecular and cellular biology

authors： Flemington EK,Lytle JP,Cayrol C,Borras AM,Speck SH

更新日期：1994-05-01 00:00:00

abstract：:The Saccharomyces cerevisiae pheromone a-factor is produced by a cells and interacts with alpha cells to cause cell cycle arrest and other physiological responses associated with mating. Two a-factor structural genes, MFA1 and MFA2, have been previously cloned with synthetic probes based on the a-factor amino acid seq...

journal_title：Molecular and cellular biology

authors： Michaelis S,Herskowitz I

更新日期：1988-03-01 00:00:00

abstract：:In the present study, we addressed the role of the c-jun proto-oncogene in the mitogenic response of human fibroblasts and primary acute myelogenous leukemia blasts to tumor necrosis factor alpha (TNF-alpha). Our data indicate that TNF-alpha treatment of these cells is associated with transcriptional activation of c-j...

journal_title：Molecular and cellular biology

authors： Brach MA,Gruss HJ,Sott C,Herrmann F

更新日期：1993-07-01 00:00:00

abstract：:The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B). In most cell contexts, hPR-B functions as a transcriptional activator of progesterone-responsive genes, whereas hPR-A functions as a transcriptional inhibitor of all steroid hormone receptors...

journal_title：Molecular and cellular biology

authors： Wen DX,Xu YF,Mais DE,Goldman ME,McDonnell DP

更新日期：1994-12-01 00:00:00

abstract：:B52, an essential SR protein of Drosophila melanogaster, stimulates pre-mRNA splicing in splicing-deficient mammalian S100 extracts. Surprisingly, mutant larvae depleted of B52 were found to be capable of splicing at least several pre-mRNAs tested (H. Z. Ring and J. T. Lis, Mol. Cell. Biol. 14:7499-7506, 1994). In a h...

journal_title：Molecular and cellular biology

authors： Hoffman BE,Lis JT

更新日期：2000-01-01 00:00:00

abstract：:Nucleolin is a ubiquitous multifunctional protein involved in preribosome assembly and associated with both nucleolar chromatin in interphase and nucleolar organizer regions on metaphasic chromosomes in mitosis. Extensive nucleolin phosphorylation by a casein kinase (CKII) occurs on serine in growing cells. Here we re...

journal_title：Molecular and cellular biology

authors： Belenguer P,Caizergues-Ferrer M,Labbé JC,Dorée M,Amalric F

更新日期：1990-07-01 00:00:00

abstract：:Lactoperoxidase was used to selectively radiolabel endocytic membrane. CHO cells were incubated with enzyme at 37 degrees C for 10 min to permit lactoperoxidase internalization. Radioiodination was done at 4 degrees C. About 90% of the radioiodinated products pelleted at 100,000 X g. From 12 to 15 different electropho...

journal_title：Molecular and cellular biology

authors： Storrie B,Dreesen TD,Maurey KM

更新日期：1981-03-01 00:00:00

abstract：:Using an expression cDNA cloning approach, we examined human tumor cell lines for novel oncogenes that might evade detection by conventional techniques. We isolated a transforming sequence that was highly efficient in transforming NIH 3T3 mouse fibroblasts. DNA sequence analysis identified the gene as the human homolo...

journal_title：Molecular and cellular biology

authors： Chan AM,Fleming TP,McGovern ES,Chedid M,Miki T,Aaronson SA

更新日期：1993-02-01 00:00:00

abstract：:The 3' noncoding region element (AUUUA)n specifically targets many short-lived mRNAs for degradation. Although the mechanism by which this sequence functions is not yet understood, a potential link between facilitated mRNA turnover and translation has been implied by the stabilization of cellular mRNAs in the presence...

journal_title：Molecular and cellular biology

authors： Aharon T,Schneider RJ

更新日期：1993-03-01 00:00:00

abstract：:Hydroxyurea (HU) is a DNA replication inhibitor that negatively affects both the elongation and initiation phases of replication and triggers the "intra-S phase checkpoint." Previous work with budding yeast has shown that, during a short exposure to HU, MEC1/RAD53 prevent initiation at some late S phase origins. In th...

journal_title：Molecular and cellular biology

authors： Alvino GM,Collingwood D,Murphy JM,Delrow J,Brewer BJ,Raghuraman MK

更新日期：2007-09-01 00:00:00

abstract：:pp60c-src was localized in the cerebellum of developing chicken embryos by immunoperoxidase staining with antisera raised against bacterially expressed pp60v-src. Immunoreactivity (IR) appeared in the cerebellum of the chicken embryos at the time of neuronal differentiation. pp60c-src IR was detected in regions of the...

journal_title：Molecular and cellular biology

authors： Fults DW,Towle AC,Lauder JM,Maness PF

更新日期：1985-01-01 00:00:00

abstract：:The murine lambda5-VpreB1 locus encodes two proteins that form part of the pre-B-cell receptor and play a key role in B-lymphocyte development. We have identified a locus control region (LCR) which is responsible for coordinate activation of both genes in pre-B cells. Analysis of mice with single and multiple copies o...

journal_title：Molecular and cellular biology

authors： Sabbattini P,Georgiou A,Sinclair C,Dillon N

更新日期：1999-01-01 00:00:00

abstract：:Cks1 is an activator of the SCF(Skp2) ubiquitin ligase complex that targets the cell cycle inhibitor p27(Kip1) for degradation. The loss of Cks1 results in p27(Kip1) accumulation and decreased proliferation and inhibits tumorigenesis. We identify here a function of Cks1 in mammalian cell cycle regulation that is indep...

journal_title：Molecular and cellular biology

authors： Hoellein A,Graf S,Bassermann F,Schoeffmann S,Platz U,Hölzlwimmer G,Kröger M,Peschel C,Oostendorp R,Quintanilla-Fend L,Keller U

更新日期：2012-07-01 00:00:00

abstract：:A set of genes is rapidly inducible when quiescent fibroblasts are stimulated by growth factors or by the activation of temperature-sensitive retroviral protein-tyrosine kinases. Most of these so-called immediate-early genes were cloned by differential cDNA hybridization. DNA sequence analysis identified many of them ...

journal_title：Molecular and cellular biology

authors： Jähner D,Hunter T

更新日期：1991-07-01 00:00:00

abstract：:The degradation of many proteins involves the sequential ligation of ubiquitin molecules to the substrate to form a multiubiquitin chain linked through Lys-48 of ubiquitin. To test for the existence of alternate forms of multiubiquitin chains, we examined the effects of individually substituting each of six other Lys ...

journal_title：Molecular and cellular biology

authors： Spence J,Sadis S,Haas AL,Finley D

更新日期：1995-03-01 00:00:00

abstract：:Mammalian Ras GTPase-activating protein (GAP), p120 Ras-GAP, has been implicated as both a downregulator and effector of Ras proteins, but its precise role in Ras-mediated signal transduction pathways is unclear. To begin a genetic analysis of the role of p120 Ras-GAP we identified a homolog from the fruit fly Drosoph...

journal_title：Molecular and cellular biology

authors： Feldmann P,Eicher EN,Leevers SJ,Hafen E,Hughes DA

更新日期：1999-03-01 00:00:00

abstract：:A cis-acting enhancer element has been detected within the gag gene of several avian retroviruses, including Rous sarcoma virus, Fujinami sarcoma virus, and the endogenous Rous-associated virus-0. A consensus enhancer core sequence, GTGGTTTG, is present in all of these viral genomes, approximately 900 bases downstream...

journal_title：Molecular and cellular biology

authors： Arrigo S,Yun M,Beemon K

更新日期：1987-01-01 00:00:00

abstract：:The protein kinase inhibitor 2-aminopurine (2-AP) greatly stimulated expression in human promonocytes-macrophages of plasmid constructs carrying various reporter genes (chloramphenicol acetyltransferase, lacZ, firefly luciferase [luc], and Salmonella typhimurium histidinol dehydrogenase [his]) driven by the human immu...

journal_title：Molecular and cellular biology

authors： Brown FL,Tahaoglu E,Graham GJ,Maio JJ

更新日期：1993-09-01 00:00:00

abstract：:The levels of intramolecular plasmid recombination, following transfection of a plasmid substrate for homologous recombination into normal and immortally transformed cells, have been examined by two independent assays. In the first assay, recovered plasmid was tested for DNA rearrangements which regenerate a functiona...

journal_title：Molecular and cellular biology

authors： Finn GK,Kurz BW,Cheng RZ,Shmookler Reis RJ

更新日期：1989-09-01 00:00:00