A ubiquitin mutant with specific defects in DNA repair and multiubiquitination.

abstract：:The TATA-binding protein (TBP), TFIIA, and TFIIB interact with promoter DNA to form a complex required for transcriptional initiation, and many transcriptional regulators function by either stimulating or inhibiting formation of this complex. We have recently identified TBP mutants that are viable in wild-type cells b...

journal_title：Molecular and cellular biology

authors： Biswas D,Imbalzano AN,Eriksson P,Yu Y,Stillman DJ

更新日期：2004-09-01 00:00:00

abstract：:Murine embryonic stem (ES) cells are defined by continuous self-renewal and pluripotency. A diverse repertoire of protein isoforms arising from alternative splicing is expressed in ES cells without defined biological roles. Sall4, a transcription factor essential for pluripotency, exists as two isoforms (Sall4a and Sa...

journal_title：Molecular and cellular biology

authors： Rao S,Zhen S,Roumiantsev S,McDonald LT,Yuan GC,Orkin SH

更新日期：2010-11-01 00:00:00

abstract：:The cytokines tumor necrosis factor (TNF), beta interferon (IFN-beta), and IFN-gamma increase major histocompatibility complex class I molecule expression. A greater than additive (i.e., synergistic) induction of class I heavy-chain mRNA is observed in HeLa cells treated with TNF in combination with either type of IFN...

journal_title：Molecular and cellular biology

authors： Johnson DR,Pober JS

更新日期：1994-02-01 00:00:00

abstract：:The transcriptional coactivators p300 and CREB binding protein (CBP) are important regulators of the cell cycle, differentiation, and tumorigenesis. Both p300 and CBP are targeted by viral oncoproteins, are mutated in certain forms of cancer, are phosphorylated in a cell cycle-dependent manner, interact with transcrip...

journal_title：Molecular and cellular biology

authors： Snowden AW,Anderson LA,Webster GA,Perkins ND

更新日期：2000-04-01 00:00:00

abstract：:We set up an alternative splicing system in vitro in which the relative amounts of two spliced RNAs, one containing and the other lacking a particular exon, were directly proportional to the length of an inverted repeat inserted into the flanking introns. We then used the system to measure the effect of intramolecular...

journal_title：Molecular and cellular biology

authors： Solnick D,Lee SI

更新日期：1987-09-01 00:00:00

abstract：:Translation is a fundamental step in gene expression, and translational control is exerted in many developmental processes. Most eukaryotic mRNAs are translated by a cap-dependent mechanism, which requires recognition of the 5'-cap structure of the mRNA by eukaryotic translation initiation factor 4E (eIF4E). eIF4E act...

journal_title：Molecular and cellular biology

authors： Hernández G,Miron M,Han H,Liu N,Magescas J,Tettweiler G,Frank F,Siddiqui N,Sonenberg N,Lasko P

更新日期：2013-08-01 00:00:00

abstract：:In cystic fibrosis (CF), epithelial cells are unable to normally up-regulate apical membrane Cl- secretion in response to agents which increase cyclic AMP, but they do increase Cl- secretion in response to increases in intracellular Ca2+. Since intracellular divalent cations regulate the expression of many genes, we h...

journal_title：Molecular and cellular biology

authors： Bargon J,Trapnell BC,Chu CS,Rosenthal ER,Yoshimura K,Guggino WB,Dalemans W,Pavirani A,Lecocq JP,Crystal RG

更新日期：1992-04-01 00:00:00

abstract：:The full-length normal and T24 mutant human H-ras proteins and two truncated derivatives of the T24 mutant were expressed efficiently in Escherichia coli. The proteins accumulated to 1 to 5% of total cellular protein, and each was specifically recognized by anti-ras monoclonal antibodies. The two full-length proteins ...

journal_title：Molecular and cellular biology

authors： Gross M,Sweet RW,Sathe G,Yokoyama S,Fasano O,Goldfarb M,Wigler M,Rosenberg M

更新日期：1985-05-01 00:00:00

abstract：:Dexamethasone increases type 1 plasminogen activator inhibitor (PAI-1) activity released from the human fibrosarcoma cell line HT-1080. We demonstrated that dexamethasone caused about 10-fold increases in the intracellular and extracellular levels of PAI-1 protein, as measured by an enzyme-linked immunosorbent assay, ...

journal_title：Molecular and cellular biology

authors： Andreasen PA,Pyke C,Riccio A,Kristensen P,Nielsen LS,Lund LR,Blasi F,Danø K

更新日期：1987-08-01 00:00:00

abstract：:S-Tubercidinylhomocysteine (STH) is a structural analog of S-adenosylhomocysteine and a potent inhibitor of S-adenosylmethionine-dependent methyltransferase reactions. We investigated the effects of STH on HeLa cell mRNA metabolism. Dual labeling studies reveal that STH dramatically inhibits the methylation of HeLa mR...

journal_title：Molecular and cellular biology

authors： Camper SA,Albers RJ,Coward JK,Rottman FM

更新日期：1984-03-01 00:00:00

abstract：:By using a model system for cell transformation mediated by the cooperation of the activated H-ras oncogene and the inactivated p53 tumor suppressor gene, rCop-1 was identified by mRNA differential display as a gene whose expression became lost after cell transformation. Homology analysis indicates that rCop-1 belongs...

journal_title：Molecular and cellular biology

authors： Zhang R,Averboukh L,Zhu W,Zhang H,Jo H,Dempsey PJ,Coffey RJ,Pardee AB,Liang P

更新日期：1998-10-01 00:00:00

abstract：:The levels of molecular chaperones and folding enzymes in the endoplasmic reticulum (ER) are controlled by a transcriptional induction process termed the unfolded protein response (UPR). The mammalian UPR is mediated by the cis-acting ER stress response element (ERSE), the consensus sequence of which is CCAAT-N(9)-CCA...

journal_title：Molecular and cellular biology

authors： Yoshida H,Okada T,Haze K,Yanagi H,Yura T,Negishi M,Mori K

更新日期：2001-02-01 00:00:00

abstract：:Approximately half of the introns in Drosophila melanogaster are too small to function in a vertebrate and often lack the pyrimidine tract associated with vertebrate 3' splice sites. Here, we report the splicing and spliceosome assembly properties of two such introns: one with a pyrimidine-poor 3' splice site and one ...

journal_title：Molecular and cellular biology

authors： Talerico M,Berget SM

更新日期：1994-05-01 00:00:00

abstract：:We have studied the patterns of expression of four B-type cyclins (Clbs), Clb1, Clb2, Clb3, and Clb4, and their ability to activate p34cdc28 during the mitotic and meiotic cell cycles of Saccharomyces cerevisiae. During the mitotic cell cycle, Clb3 and Clb4 were expressed and induced a kinase activity in association w...

journal_title：Molecular and cellular biology

authors： Grandin N,Reed SI

更新日期：1993-04-01 00:00:00

abstract：:This paper presents a general means of eliminating the function of a single protein without relying on genetic alterations in its structure or level of synthesis. The strategy is based on the inducible cellular expression of neutralizing antibody to inactivate the protein selectively. The feasibility of this approach ...

journal_title：Molecular and cellular biology

authors： Carlson JR

更新日期：1988-06-01 00:00:00

abstract：:An accumulation in cells of unfolded proteins is believed to be the common signal triggering the induction of heat shock proteins (hsps). Accordingly, in Saccharomyces cerevisiae, inhibition of protein breakdown at 30 degrees C with the proteasome inhibitor MG132 caused a coordinate induction of many heat shock protei...

journal_title：Molecular and cellular biology

authors： Lee DH,Goldberg AL

更新日期：1998-01-01 00:00:00

abstract：:Both systemic insulin resistance and tissue-specific insulin resistance have been described in Drosophila and are accompanied by many indicators of metabolic disease. The downstream mediators of insulin-resistant pathophysiology remain unclear. We analyzed insulin signaling in the fat body studying loss and gain of fu...

journal_title：Molecular and cellular biology

authors： Musselman LP,Fink JL,Grant AR,Gatto JA,Tuthill BF 2nd,Baranski TJ

更新日期：2017-12-29 00:00:00

abstract：:The tissue-specific expression of immunoglobulin genes can be partially explained by a requirement for activating factors found only in B lymphocytes and their derivatives. However, loss of immunoglobulin expression upon fusion of an immunoglobulin-producing myeloma cell with a T lymphoma cell (BW5147) or fibroblast (...

journal_title：Molecular and cellular biology

authors： Zaller DM,Yu H,Eckhardt LA

更新日期：1988-05-01 00:00:00

abstract：:Prostaglandins, pleiotropic immune modulators that induce protein kinase A (PKA), inhibit gamma interferon induction of class II major histocompatibility complex (MHC) genes. We show that phosphorylation of CIITA by PKA accounts for this inhibition. Treatment with prostaglandin E or 8-bromo-cyclic AMP or transfection ...

journal_title：Molecular and cellular biology

authors： Li G,Harton JA,Zhu X,Ting JP

更新日期：2001-07-01 00:00:00

abstract：:The SNF3 gene of Saccharomyces cerevisiae encodes a high-affinity glucose transporter that is homologous to mammalian glucose transporters. Point mutations affecting the function of the transporter were recovered from the genomes of four snf3 mutants and characterized. Two of the mutations introduced a charged amino a...

journal_title：Molecular and cellular biology

authors： Marshall-Carlson L,Celenza JL,Laurent BC,Carlson M

更新日期：1990-03-01 00:00:00

abstract：:p53 promotes apoptosis in response to death stimuli by transactivation of target genes and by transcription-independent mechanisms. We recently showed that wild-type p53 rapidly translocates to mitochondria in response to multiple death stimuli in cultured cells. Mitochondrial p53 physically interacts with antiapoptot...

journal_title：Molecular and cellular biology

authors： Erster S,Mihara M,Kim RH,Petrenko O,Moll UM

更新日期：2004-08-01 00:00:00

abstract：:The mouse Surfeit locus, which contains a cluster of at least four genes (Surf-1 to Surf-4), is unusual in that adjacent genes are separated by no more than 73 base pairs (bp). The heterogeneous 5' ends of Surf-1 and Surf-2 are separated by only 15 to 73 bp, the 3' ends of Surf-1 and Surf-3 are only 70 bp apart, and t...

journal_title：Molecular and cellular biology

authors： Giallongo A,Yon J,Fried M

更新日期：1989-01-01 00:00:00

abstract：:F8dl is a simian virus 40 early-region deletion mutant that lacks the sequences between 0.169 and 0.423 map units. We show that cloned F8dl DNA immortalized early-passage Fisher rat embryo cells with an efficiency that was about 20% of that of cloned wild-type simian virus 40 DNA. In contrast, we detected no immortali...

journal_title：Molecular and cellular biology

authors： Sompayrac L,Danna KJ

更新日期：1985-05-01 00:00:00

abstract：:The Drosophila melanogaster GAGA factor (encoded by the Trithorax-like [Trl] gene) is required for correct chromatin architecture at diverse chromosomal sites. The Trl gene encodes two alternatively spliced isoforms of the GAGA factor (GAGA-519 and GAGA-581) that are identical except for the length and sequence of the...

journal_title：Molecular and cellular biology

authors： Greenberg AJ,Schedl P

更新日期：2001-12-01 00:00:00

abstract：:By immunoblotting with antibodies for phosphotyrosine, we have demonstrated that the hematopoietic growth factors interleukin-2, interleukin-3, interleukin-4, and granulocyte-macrophage colony-stimulating factor stimulate the tyrosine phosphorylation of specific sets of proteins in murine hematopoietic progenitor cell...

journal_title：Molecular and cellular biology

authors： Morla AO,Schreurs J,Miyajima A,Wang JY

更新日期：1988-05-01 00:00:00

abstract：:CAP, a protein from Saccharomyces cerevisiae that copurifies with adenylyl cyclase, appears to be required for yeast cells to be fully responsive to RAS proteins. CAP also appears to be required for normal cell morphology and responsiveness to nutrient deprivation and excess. We describe here a molecular and phenotypi...

journal_title：Molecular and cellular biology

authors： Gerst JE,Ferguson K,Vojtek A,Wigler M,Field J

更新日期：1991-03-01 00:00:00

abstract：:The effect of atenolol, propranolol, trifluoperazine, and caffeine on the occurrence of meiotic diploid and disomic products in Saccharomyces cerevisiae was investigated. We demonstrated that atenolol, propranolol, and trifluoperazine reduce the occurrence of meiotic diploid products and that propranolol also slightly...

journal_title：Molecular and cellular biology

authors： Sora S,Bianchi M

更新日期：1982-11-01 00:00:00

abstract：:TIN2 is an important regulator of telomere length, and mutations in TINF2, the gene encoding TIN2, cause short-telomere syndromes. While the genetics underscore the importance of TIN2, the mechanism through which TIN2 regulates telomere length remains unclear. Here, we tested the effects of human TIN2 on telomerase ac...

journal_title：Molecular and cellular biology

authors： Pike AM,Strong MA,Ouyang JPT,Greider CW

更新日期：2019-10-11 00:00:00

abstract：:Wound healing in certain individuals leads to the development of keloid tumors which exhibit abnormal collagen metabolism and an increased abundance of extracellular matrix components. Comparison of fibronectin levels in fibroblasts derived from keloids and normal dermis revealed a relative increase in intracellular a...

journal_title：Molecular and cellular biology

authors： Babu M,Diegelmann R,Oliver N

更新日期：1989-04-01 00:00:00

abstract：:The full-length versions of the Ras-specific exchange factors Ras-GRF1 (GRF1) and Ras-GRF2 (GRF2), which are expressed in brain and a restricted number of other organs, possess an ionomycin-dependent activation of Erk mitogen-activated protein kinase activity in 293T cells (C. L. Farnsworth et al., Nature 376:524-527,...

journal_title：Molecular and cellular biology

authors： Anborgh PH,Qian X,Papageorge AG,Vass WC,DeClue JE,Lowy DR

更新日期：1999-07-01 00:00:00