A novel transcriptional repression domain mediates p21(WAF1/CIP1) induction of p300 transactivation.

abstract：:The Tor pathway mediates cell growth in response to nutrient availability, in part by inducing ribosomal protein (RP) gene expression via an unknown mechanism. Expression of RP genes coincides with recruitment of the Esa1 histone acetylase to RP gene promoters. We show that inhibition of Tor with rapamycin releases Es...

journal_title：Molecular and cellular biology

authors： Rohde JR,Cardenas ME

更新日期：2003-01-01 00:00:00

abstract：:The RNA-binding protein (RBP) nucleolin promotes the expression of several proliferative proteins. Nucleolin levels are high in cancer cells, but the mechanisms that control nucleolin expression are unknown. Here, we show that nucleolin abundance is controlled posttranscriptionally via factors that associate with its ...

journal_title：Molecular and cellular biology

authors： Tominaga K,Srikantan S,Lee EK,Subaran SS,Martindale JL,Abdelmohsen K,Gorospe M

更新日期：2011-10-01 00:00:00

abstract：:The c-ABL tyrosine kinase is activated following either the loss or mutation of its Src homology domain 3 (SH3), resulting in both increased autophosphorylation and phosphorylation of cellular substrates and cellular transformation. This suggests that the SH3 domain negatively regulates c-ABL kinase activity. For seve...

journal_title：Molecular and cellular biology

authors： Zhu J,Shore SK

更新日期：1996-12-01 00:00:00

abstract：:The signal-transducing adaptor protein 2 (STAP-2) is a recently identified adaptor protein that contains a pleckstrin homology (PH) and Src homology 2 (SH2)-like domains, as well as a proline-rich domain in its C-terminal region. In previous studies, we demonstrated that STAP-2 binds to MyD88 and IKK-alpha or IKK-beta...

journal_title：Molecular and cellular biology

authors： Ikeda O,Sekine Y,Yasui T,Oritani K,Sugiyma K,Muromoto R,Ohbayashi N,Yoshimura A,Matsuda T

更新日期：2008-08-01 00:00:00

abstract：:To investigate the role of insulin receptor substrate 1 (IRS-1) and IRS-2, the two ubiquitously expressed IRS proteins, in adipocyte differentiation, we established embryonic fibroblast cells with four different genotypes, i.e., wild-type, IRS-1 deficient (IRS-1(-/-)), IRS-2 deficient (IRS-2(-/-)), and IRS-1 IRS-2 dou...

journal_title：Molecular and cellular biology

authors： Miki H,Yamauchi T,Suzuki R,Komeda K,Tsuchida A,Kubota N,Terauchi Y,Kamon J,Kaburagi Y,Matsui J,Akanuma Y,Nagai R,Kimura S,Tobe K,Kadowaki T

更新日期：2001-04-01 00:00:00

abstract：:To explore the diversity in the mechanisms of basal transcription by RNA polymerase II, we have employed a novel biochemical approach that involves perturbation of the transcription reaction with exogenously added TFIIB or TATA box-binding protein (TBP). Under these conditions, we observe promoter-selective inhibition...

journal_title：Molecular and cellular biology

authors： George CP,Lira-DeVito LM,Wampler SL,Kadonaga JT

更新日期：1995-02-01 00:00:00

abstract：:Transcriptional factors of the NFAT family play an important role in regulating the expression of several cytokine genes during the immune response, such as the genes for interleukin 2 (IL-2) and IL-4, among others. Upon antigen stimulation, precursor CD4+ T helper (pTh) cells proliferate and differentiate into two po...

journal_title：Molecular and cellular biology

authors： Rincón M,Flavell RA

更新日期：1997-03-01 00:00:00

abstract：:We analyzed the binding site(s) for Grb2 on the epidermal growth factor (EGF) receptor (EGFR), using cell lines overexpressing EGFRs containing various point and deletion mutations in the carboxy-terminal tail. Results of co-immunoprecipitation experiments suggest that phosphotyrosines Y-1068 and Y-1173 mediate the bi...

journal_title：Molecular and cellular biology

authors： Batzer AG,Rotin D,Ureña JM,Skolnik EY,Schlessinger J

更新日期：1994-08-01 00:00:00

abstract：:The cytoplasm of Saccharomyces cerevisiae contains two major classes of protein-encapsulated double-stranded ribonucleic acids (dsRNA's), L and M. Replication of L and M dsRNA's was examined in cells arrested in the G1 phase by either alpha-factor, a yeast mating pheromone, or the restrictive temperature for a cell cy...

journal_title：Molecular and cellular biology

authors： Zakian VA,Wagner DW,Fangman WL

更新日期：1981-08-01 00:00:00

abstract：:The RAD1 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of damaged DNA. In this paper, we report our observations on the effect of the RAD1 gene on genetic recombination. Mitotic intrachromosomal and interchromosomal recombination in RAD+, rad1, rad52, and other rad mutant strain...

journal_title：Molecular and cellular biology

authors： Schiestl RH,Prakash S

更新日期：1988-09-01 00:00:00

abstract：:SUMO conjugation has emerged as a dynamic process in regulating protein function. Here we identify estrogen receptor β (ERβ) to be a new target of SUMO-1. ERβ SUMO-1 modification occurs on a unique nonconsensus sumoylation motif which becomes fully competent upon phosphorylation of its contained serine residue, which ...

journal_title：Molecular and cellular biology

authors： Picard N,Caron V,Bilodeau S,Sanchez M,Mascle X,Aubry M,Tremblay A

更新日期：2012-07-01 00:00:00

abstract：:Elevation of cellular cyclic AMP (cAMP) levels inhibits cell cycle reentry in a variety of cell types. While cAMP can prevent the activation of Raf-1 and extracellular signal-regulated kinases 1 and 2 (ERK1/2) by growth factors, we now show that activation of ERK1/2 by DeltaRaf-1:ER is insensitive to cAMP. Despite thi...

journal_title：Molecular and cellular biology

authors： Balmanno K,Millar T,McMahon M,Cook SJ

更新日期：2003-12-01 00:00:00

abstract：:Two alleles of the Drosophila melanogaster Rfc4 (DmRfc4) gene, which encodes subunit 4 of the replication factor C (RFC) complex, cause striking defects in mitotic chromosome cohesion and condensation. These mutations produce larval phenotypes consistent with a role in DNA replication but also result in mitotic chromo...

journal_title：Molecular and cellular biology

authors： Krause SA,Loupart ML,Vass S,Schoenfelder S,Harrison S,Heck MM

更新日期：2001-08-01 00:00:00

abstract：:Fibroblast cultures prepared from mice homozygous for a Robertsonian translocation (centric fusion) between autosomes 8 and 17 [Rb(8.17)] were used as donors in microcell-mediated chromosome transfer experiments. By using hamster recipient cells deficient in adenine phosphoribosyltransferase (APRT-) and selecting for ...

journal_title：Molecular and cellular biology

authors： Fournier RE,Frelinger JA

更新日期：1982-05-01 00:00:00

abstract：:Secretory proteins in eukaryotic cells are transported to the cell surface via the endoplasmic reticulum (ER) and the Golgi apparatus by membrane-bounded vesicles. We screened a collection of temperature-sensitive mutants of Saccharomyces cerevisiae for defects in ER-to-Golgi transport. Two of the genes identified in ...

journal_title：Molecular and cellular biology

authors： Chen EJ,Frand AR,Chitouras E,Kaiser CA

更新日期：1998-12-01 00:00:00

abstract：:The large T antigen of simian virus 40 (SV40) is a multifunctional protein that is essential in both the virus lytic cycle and the oncogenic transformation of cells by SV40. To investigate the role of the numerous biochemical and physiological activities of T antigen in the lytic and transformation processes, we have ...

journal_title：Molecular and cellular biology

authors： Manos MM,Gluzman Y

更新日期：1984-06-01 00:00:00

abstract：:Fragments of the TAR DNA-binding protein 43 (TDP43) are major components of intracellular aggregates associated with amyotrophic lateral sclerosis and frontotemporal dementia. A variety of C-terminal fragments (CTFs) exist, with distinct N termini; however, little is known regarding their differences in metabolism and...

journal_title：Molecular and cellular biology

authors： Kasu YAT,Alemu S,Lamari A,Loew N,Brower CS

更新日期：2018-09-14 00:00:00

abstract：:Transcription of the Drosophila homologs of the abl and src cellular oncogenes, termed Dash and Dsrc , respectively, was studied. Despite the fact that Dash and Dsrc share sequence homology, they have distinct patterns of transcription during development. The Dash transcript, 6.2 kilobases long, is found in maternal R...

journal_title：Molecular and cellular biology

authors： Lev Z,Leibovitz N,Segev O,Shilo BZ

更新日期：1984-05-01 00:00:00

abstract：:Our analysis of rotund (rn) null mutations in Drosophila melanogaster revealed that deletion of the rn locus affects both spermatid and retinal differentiation. In the male reproductive system, the absence of RnRacGAP induced small testes, empty seminal vesicles, short testicular cysts, reduced amounts of interspermat...

journal_title：Molecular and cellular biology

authors： Bergeret E,Pignot-Paintrand I,Guichard A,Raymond K,Fauvarque MO,Cazemajor M,Griffin-Shea R

更新日期：2001-09-01 00:00:00

abstract：:Compromised immunoregulation contributes to obesity and complications in metabolic pathogenesis. Here, we demonstrate that the nuclear factor of activated T cell (NFAT) group of transcription factors contributes to glucose and insulin homeostasis. Expression of two members of the NFAT family (NFATc2 and NFATc4) is ind...

journal_title：Molecular and cellular biology

authors： Yang TT,Suk HY,Yang X,Olabisi O,Yu RY,Durand J,Jelicks LA,Kim JY,Scherer PE,Wang Y,Feng Y,Rossetti L,Graef IA,Crabtree GR,Chow CW

更新日期：2006-10-01 00:00:00

abstract：:In Saccharomyces cerevisiae, pseudouridine formation in the middle position of the tRNA(Tyr) anticodon (psi 35) is dependent on the presence of the intron in the tRNA(Tyr) gene (Johnson and Abelson, Nature 302:681-687, 1983). Drosophila melanogaster tRNA(Tyr) genes contain introns of three size classes: 20 or 21 base ...

journal_title：Molecular and cellular biology

authors： Choffat Y,Suter B,Behra R,Kubli E

更新日期：1988-08-01 00:00:00

abstract：:The carcinoembryonic antigen (CEA) family consists of a large group of evolutionarily and structurally divergent glycoproteins. The murine CEACAM9 and CEACAM11-related proteins as well as the pregnancy-specific glycoproteins (PSG) are secreted members of the CEA family which are differentially expressed in fetal troph...

journal_title：Molecular and cellular biology

authors： Finkenzeller D,Fischer B,Lutz S,Schrewe H,Shimizu T,Zimmermann W

更新日期：2003-01-01 00:00:00

abstract：:CD40, a tumor necrosis factor (TNF) receptor (TNFR) family member, conveys signals regulating diverse cellular responses, ranging from proliferation and differentiation to growth suppression and cell death. The ability of CD40 to mediate apoptosis in carcinoma cells is intriguing given the fact that the CD40 cytoplasm...

journal_title：Molecular and cellular biology

authors： Eliopoulos AG,Davies C,Knox PG,Gallagher NJ,Afford SC,Adams DH,Young LS

更新日期：2000-08-01 00:00:00

abstract：:The class II transactivator (CIITA) is induced by gamma interferon (IFN-gamma) and activates major histocompatibility complex class II; however, this report shows it suppresses other genes. An N-terminal 36 amino acids of CIITA mediates suppression of the collagen alpha(2)(I) promoter via binding to CREB-binding prote...

journal_title：Molecular and cellular biology

authors： Zhu XS,Ting JP

更新日期：2001-10-01 00:00:00

abstract：:USF is a family of basic helix-loop transcriptional factors that recognizes DNA-binding sites similar to those of the Myc oncoproteins. Here, various functional domains in the mouse USF2 protein were identified and characterized. Indirect immunofluorescence studies with transiently transfected cells revealed that both...

journal_title：Molecular and cellular biology

authors： Luo X,Sawadogo M

更新日期：1996-04-01 00:00:00

abstract：:To understand the mechanism of retinoid resistance, we studied the subcellular localization and function of retinoid receptors in human breast cancer cell lines. Retinoid X receptor alpha (RXR alpha) localized throughout the nucleoplasm in retinoid-sensitive normal human mammary epithelial cells and in retinoid-respon...

journal_title：Molecular and cellular biology

authors： Tanaka T,Dancheck BL,Trifiletti LC,Birnkrant RE,Taylor BJ,Garfield SH,Thorgeirsson U,De Luca LM

更新日期：2004-05-01 00:00:00

abstract：:We find that immediately following transcript initiation, RNA polymerase II pauses at several locations even in the presence of relatively high (200 microM) levels of nucleoside triphosphates. Strong pauses with half-lives of >30 s were observed at +7, +18/19, and about +25 on the template used in these experiments. W...

journal_title：Molecular and cellular biology

authors： Pal M,Luse DS

更新日期：2002-01-01 00:00:00

abstract：:Transthyretin (TTR) and alpha 1-antitrypsin (alpha 1-AT) are expressed at high levels in the liver and also in at least one other cell type. We report here a detailed analysis of the proximal regulatory region of the TTR gene, which has uncovered two new DNA-binding factors that are present mainly (or only) in hepatoc...

journal_title：Molecular and cellular biology

authors： Costa RH,Grayson DR,Darnell JE Jr

更新日期：1989-04-01 00:00:00

abstract：:Smads 1, 5, and 8 are the intracellular mediators for the bone morphogenetic proteins (BMPs), which play crucial roles during mammalian development. Previous research has shown that Smad1 is important in the formation of the allantois, while Smad5 has been shown to be critical in the process of angiogenesis. To furthe...

journal_title：Molecular and cellular biology

authors： Hester M,Thompson JC,Mills J,Liu Y,El-Hodiri HM,Weinstein M

更新日期：2005-06-01 00:00:00

abstract：:The GGAs (Golgi-localized, gamma-ear-containing, ARF-binding proteins) are a family of multidomain adaptor proteins involved in protein sorting at the trans-Golgi network of eukaryotic cells. Here we present results from a functional characterization of the two Saccharomyces cerevisiae GGAs, Gga1p and Gga2p. We show t...

journal_title：Molecular and cellular biology

authors： Mullins C,Bonifacino JS

更新日期：2001-12-01 00:00:00