Abstract:
:SUMO conjugation has emerged as a dynamic process in regulating protein function. Here we identify estrogen receptor β (ERβ) to be a new target of SUMO-1. ERβ SUMO-1 modification occurs on a unique nonconsensus sumoylation motif which becomes fully competent upon phosphorylation of its contained serine residue, which provides the essential negative charge for sumoylation. This process is further regulated by phosphorylation of additional adjacent serine residues by glycogen synthase kinase 3β (GSK3β), which maximizes ERβ sumoylation in response to hormone. SUMO-1 attachment prevents ERβ degradation by competing with ubiquitin at the same acceptor site and dictates ERβ transcriptional inhibition by altering estrogen-responsive target promoter occupancy and gene expression in breast cancer cells. These findings uncovered a novel phosphorylated sumoylation motif (pSuM), which consists of the sequence ψKXS (where ψ represents a large hydrophobic residue) and which is connected to a GSK3-activated extension that functions as a SUMO enhancer. This extended pSuM offers a valuable signature to predict SUMO substrates under protein kinase regulation.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Picard N,Caron V,Bilodeau S,Sanchez M,Mascle X,Aubry M,Tremblay Adoi
10.1128/MCB.06624-11subject
Has Abstractpub_date
2012-07-01 00:00:00pages
2709-21issue
14eissn
0270-7306issn
1098-5549pii
MCB.06624-11journal_volume
32pub_type
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