Abstract:
:The effect of atenolol, propranolol, trifluoperazine, and caffeine on the occurrence of meiotic diploid and disomic products in Saccharomyces cerevisiae was investigated. We demonstrated that atenolol, propranolol, and trifluoperazine reduce the occurrence of meiotic diploid products and that propranolol also slightly decreases the spontaneous frequency of disomics. On the other hand, caffeine appears to be a powerful inducer of diploid meiotic products, but also shows a lesser effect on disomic induction. Since spontaneous or caffeine-induced diploids arise from a failure of the second meiotic division, it appears that the target of these drugs is at the beginning of the second meiotic division. The only common effect of trifluoperazine and propranolol, mainly investigated in mammals, was an inhibition of calmodulin activity via direct interaction. We tend, therefore, to believe that calmodulin activity must be a crucial point for the second meiotic division to begin. The increased induction of diploids, due to caffeine, may be interpreted as a consequence of an increased cyclic AMP level.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Sora S,Bianchi Mdoi
10.1128/mcb.2.11.1299subject
Has Abstractpub_date
1982-11-01 00:00:00pages
1299-303issue
11eissn
0270-7306issn
1098-5549journal_volume
2pub_type
杂志文章abstract::Saccharomyces cerevisiae mating type switching is a gene conversion event that exhibits donor preference. MATa cells choose HMLalpha for recombination, and MATalpha cells choose HMRa. Donor preference is controlled by the recombination enhancer (RE), located between HMLalpha and MATa on the left arm of chromosome III....
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.25.18.7976-7987.2005
更新日期:2005-09-01 00:00:00
abstract::Vascular endothelium (VE), the monolayer of endothelial cells that lines the vascular tree, undergoes damage at the basis of some vascular diseases. Its integrity is maintained by VE-cadherin, an adhesive receptor localized at cell-cell junctions. Here, we show that VE-cadherin is also located at the tip and along fil...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01226-09
更新日期:2010-04-01 00:00:00
abstract::The Moloney murine sarcoma virus long terminal repeat (LTR) harbors two distinct positive activators of transcription, namely, a distal signal and an enhancer. In this report we demonstrate that infection by herpes simplex virus (HSV) can markedly affect the utilization of these two Moloney murine sarcoma virus transc...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.5.8.1959
更新日期:1985-08-01 00:00:00
abstract::The Ras-RAF-mitogen-activated protein kinase (Ras-RAF-MAPK) pathway is overactive in many cancers and in some developmental disorders. In one of those disorders, namely, Noonan syndrome, nine activating C-RAF mutations cluster around Ser(259), a regulatory site for inhibition by 14-3-3 proteins. We show that these mut...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01636-09
更新日期:2010-10-01 00:00:00
abstract::Retinoblastoma (RB) tumors develop when both alleles of a gene (RB1) are mutated and unable to function normally. Recently, Friend et al. [S. H. Friend, R. Bernards, S. Rogelj, R. A. Weinberg, J. M. Rapaport, D. M. Albert, and T. P. Dryja, Nature (London) 32:643-646, 1986] reported the cloning of a gene, 4.7R, with so...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.8.5.2082
更新日期:1988-05-01 00:00:00
abstract::Erythroid Krüppel-like factor (EKLF [KLF1]) is a transcriptional regulator that plays a critical role within a specific subset of hematopoietic cells, particularly in the erythroid lineage and its immediate precursor, the megakaryocyte-erythroid progenitor (MEP). We find that EKLF is posttranslationally modified by su...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00589-07
更新日期:2007-12-01 00:00:00
abstract::SLC5A8 is a putative tumor suppressor that is inactivated in more than 10 different types of cancer, but neither the oncogenic signaling responsible for SLC5A8 inactivation nor the functional relevance of SLC5A8 loss to tumor growth has been elucidated. Here, we identify oncogenic HRAS (HRAS(G12V)) as a potent mediato...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01702-12
更新日期:2013-10-01 00:00:00
abstract::EYA1 is known to be overexpressed in human breast cancer, in which the Myc protein is also accumulated in association with decreased phospho-T58 (pT58) levels. We have recently reported that EYA1 functions as a unique protein phosphatase to dephosphorylate Myc at pT58 to regulate Myc levels. However, it remains unclea...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00499-16
更新日期:2016-12-19 00:00:00
abstract::Previous characterization of GLI, a gene found to be amplified and expressed in a subset of human brain tumors, revealed the presence of five tandem zinc fingers related to those of Krüppel (Kr), a Drosophila segmentation gene of the gap class. We have used the GLI cDNA as a molecular probe to isolate related sequence...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.8.8.3104
更新日期:1988-08-01 00:00:00
abstract::The UvsW protein of bacteriophage T4 is involved in many aspects of phage DNA metabolism, including repair, recombination, and recombination-dependent replication. UvsW has also been implicated in the repression of origin-dependent replication at late times of infection, when UvsW is normally synthesized. Two well-cha...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.21.8.2706-2715.2001
更新日期:2001-04-01 00:00:00
abstract::The human alpha-tropomyosin gene hTMnm has two mutually exclusive versions of exon 5 (NM and SK), one of which is expressed specifically in skeletal muscle (exon SK). A minigene construct expresses only the nonmuscle (NM) isoform when transfected into COS-1 cells and both forms when transfected into myoblasts. Twenty-...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.12.9.3872
更新日期:1992-09-01 00:00:00
abstract::As a subunit of a ubiquitin ligase, Skp2 is implicated in facilitating cell cycle progression via degradation of various protein targets. We report here that Skp2 is rapidly degraded following cellular stimulation by the cytokine transforming growth factor beta (TGF-beta) and that this degradation stabilizes the cell ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01830-06
更新日期:2007-04-01 00:00:00
abstract::The positively acting regulatory gene amdR of Aspergillus nidulans coordinately regulates the expression of five structural genes involved in the catabolism of certain amides (amdS), omega amino acids (gatA and gabA), and lactams (lamA and lamB) in the presence of omega amino acid inducers. Analysis of the amdR gene s...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.10.6.3194
更新日期:1990-06-01 00:00:00
abstract::Nucleolar morphogenesis is a poorly defined process. Here we report that the Saccharomyces cerevisiae nucleolar trimethyl guanosine synthase I (Tgs1p), which specifically selects the m(7)G cap structure of snRNAs and snoRNAs for m(2,2,7)G conversion, is required not only for efficient pre-mRNA splicing but also for pr...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.24.18.7976-7986.2004
更新日期:2004-09-01 00:00:00
abstract::A murine monoclonal antibody was identified by its ability to induce a reversible antiproliferative effect on a human lymphoma cell line. Immunoprecipitation studies revealed that the antibody reacted with a 26-kilodalton cell surface protein (TAPA-1). A diverse group of human cell lines, including hematolymphoid, neu...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.10.8.4007
更新日期:1990-08-01 00:00:00
abstract::We have characterized the role of YPR128cp, the orthologue of human PMP34, in fatty acid metabolism and peroxisomal proliferation in Saccharomyces cerevisiae. YPR128cp belongs to the mitochondrial carrier family (MCF) of solute transporters and is localized in the peroxisomal membrane. Disruption of the YPR128c gene r...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.21.13.4321-4329.2001
更新日期:2001-07-01 00:00:00
abstract::Defects in mismatch repair (MMR) genes result in a mutator phenotype by inducing microsatellite instability (MI), a characteristic of hereditary nonpolyposis colorectal cancers (HNPCC) and a subset of sporadic colon tumors. Present models describing the mechanism by which germ line mutations in MMR genes predispose ki...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.18.3.1635
更新日期:1998-03-01 00:00:00
abstract::We have used extrachromosomal substrates carrying immunoglobulin heavy-chain S mu and S gamma 3 switch region sequences to study activation and targeting of recombination by a transcriptional enhancer element. Substrates are transiently introduced into activated primary murine B cells, in which recombination involving...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.14.2.1450
更新日期:1994-02-01 00:00:00
abstract::GIPC is a PDZ protein located on peripheral endosomes that binds to the juxtamembrane region of the TrkA nerve growth factor (NGF) receptor and has been implicated in NGF signaling. We establish here that endogenous GIPC binds to the C terminus of APPL, a Rab5 binding protein, which is a marker for signaling endosomes...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00305-06
更新日期:2006-12-01 00:00:00
abstract::Integrin-mediated interactions of cells with components of the extracellular matrix regulate cell survival, cell proliferation, cell differentiation, and cell migration. Some of these physiological responses are regulated via activation of transcription factors such as activator protein 1 (AP-1). Integrin-linked kinas...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.19.11.7420
更新日期:1999-11-01 00:00:00
abstract::All inbred strains of mice carry the Ren-1 structural gene, which encodes the renin-1 isozyme, the classical renin activity found in kidneys. In addition, some strains carry a second renin structural gene, Ren-2, which encodes the predominantly expressed submaxillary gland renin isozyme, renin-2. Ren-1 and Ren-2 exhib...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.4.11.2321
更新日期:1984-11-01 00:00:00
abstract::Following a proliferative phase of variable duration, most normal somatic cells enter a growth arrest state known as replicative senescence. In addition to telomere shortening, a variety of environmental insults and signaling imbalances can elicit phenotypes closely resembling senescence. We used p53(-/-) and p21(-/-)...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.21.20.6748-6757.2001
更新日期:2001-10-01 00:00:00
abstract::Viruses have developed numerous strategies to counteract the host cell defense. Kaposi's sarcoma-associated herpesvirus (KSHV) is a DNA tumor virus linked to the development of Kaposi's sarcoma, Castleman's disease, and primary effusion lymphoma (PEL). The virus-encoded viral interferon regulatory factor 3 (vIRF-3) ge...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01011-13
更新日期:2014-02-01 00:00:00
abstract::We examined the mechanisms that control the downregulation of the c-myc mRNA during differentiation of HL60 cells. On treatment with dimethyl sulfoxide, HL60 cells downmodulated their steady-state c-myc message levels, ceased to proliferate, and underwent terminal differentiation. In nuclear run-on assays in which dis...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.8.2.867
更新日期:1988-02-01 00:00:00
abstract::Drosophila melanogaster telomeres have two DNA domains: a terminal array of retrotransposons and a subterminal repetitive telomere-associated sequence (TAS), a source of telomere position effect (TPE). We reported previously that deletion of the 2L TAS array leads to dominant suppression of TPE by stimulating in trans...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00515-07
更新日期:2007-07-01 00:00:00
abstract::Neuronal differentiation is tightly regulated by a variety of factors. In a search for neuron-specific genes, we identified a highly conserved novel zinc finger protein, ZNF536. We observed that ZNF536 is most abundant in the brain and, in particular, is expressed in the developing central nervous system and dorsal ro...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00362-09
更新日期:2009-07-01 00:00:00
abstract::In Drosophila and several other metazoan organisms, there are two genes that encode related but distinct homologs of ADA2-type transcriptional adaptors. Here we describe mutations of the two Ada2 genes of Drosophila melanogaster. By using mutant Drosophila lines, which allow the functional study of individual ADA2s, w...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.25.18.8215-8227.2005
更新日期:2005-09-01 00:00:00
abstract::Low-molecular-weight RNA exhibiting transforming potential was identified in chemically induced lymphoma cells by the transformation of mink lung cells after transfection. The RNA was sequenced by the direct chemical method and was shown to be a small nuclear RNA, U5. The transforming potential of the RNA was further ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.9.10.4345
更新日期:1989-10-01 00:00:00
abstract::Although largely involved in innate and adaptive immunity, NF-kappa B plays an important role in vertebrate development. In chicks, the inactivation of the NF-kappa B pathway induces functional alterations of the apical ectodermal ridge, which mediates limb outgrowth. In mice, the complete absence of NF-kappa B activi...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.24.12.5257-5268.2004
更新日期:2004-06-01 00:00:00
abstract::The tyrosinase family comprises three members, tyrosinase (Tyr), tyrosinase-related protein 1 (Tyrp1), and dopachrome tautomerase (Dct). Null mutations and deletions at the Tyr and Tyrp1 loci are known and phenotypically affect coat color due to the absence of enzyme or intracellular mislocalization. At the Dct locus,...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.24.8.3396-3403.2004
更新日期:2004-04-01 00:00:00