Abstract:
:Prostaglandins, pleiotropic immune modulators that induce protein kinase A (PKA), inhibit gamma interferon induction of class II major histocompatibility complex (MHC) genes. We show that phosphorylation of CIITA by PKA accounts for this inhibition. Treatment with prostaglandin E or 8-bromo-cyclic AMP or transfection with PKA inhibits the activity of CIITA in both mouse and human monocytic cell lines. This inhibition is independent of other transcription factors for the class II MHC promoter. These same treatments also greatly reduced the induction of class II MHC mRNA by CIITA. PKA phosphorylation sites were identified using site-directed mutagenesis and phosphoamino acid analysis. Phosphorylation at CIITA serines 834 and 1050 accounts for the inhibitory effects of PKA on CIITA-driven class II MHC transcription. This is the first demonstration that the posttranslational modification of CIITA mediates inhibition of class II MHC transcription.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Li G,Harton JA,Zhu X,Ting JPdoi
10.1128/MCB.21.14.4626-4635.2001subject
Has Abstractpub_date
2001-07-01 00:00:00pages
4626-35issue
14eissn
0270-7306issn
1098-5549journal_volume
21pub_type
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pub_type: 杂志文章
doi:10.1128/MCB.00948-06
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pub_type: 杂志文章
doi:10.1128/mcb.8.10.4174
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doi:10.1128/mcb.12.3.962
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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