Abstract:
:Transcriptome profiling studies have recently uncovered a large number of noncoding RNA transcripts (ncRNAs) in eukaryotic organisms, and there is growing interest in their role in the cell. For example, in haploid Saccharomyces cerevisiae cells, the expression of an overlapping antisense ncRNA, referred to here as RME2 (Regulator of Meiosis 2), prevents IME4 expression. In diploid cells, the a1-α2 complex represses the transcription of RME2, allowing IME4 to be induced during meiosis. In this study we show that antisense transcription across the IME4 promoter region does not block transcription factors from binding and is not required for repression. Mutational analyses found that sequences within the IME4 open reading frame (ORF) are required for the repression mediated by RME2 transcription. These results support a model where transcription of RME2 blocks the elongation of the full-length IME4 transcript but not its initiation. We have found that another antisense transcript, called RME3, represses ZIP2 in a cell-type-specific manner. These results suggest that regulated antisense transcription may be a widespread mechanism for the control of gene expression and may account for the roles of some of the previously uncharacterized ncRNAs in yeast.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Gelfand B,Mead J,Bruning A,Apostolopoulos N,Tadigotla V,Nagaraj V,Sengupta AM,Vershon AKdoi
10.1128/MCB.01071-10subject
Has Abstractpub_date
2011-04-01 00:00:00pages
1701-9issue
8eissn
0270-7306issn
1098-5549pii
MCB.01071-10journal_volume
31pub_type
杂志文章abstract::The Polycomb group proteins are involved in maintenance of the silenced state of several developmentally regulated genes. These proteins form large aggregates with different subunit compositions. To explore the nature of these complexes and their function, we used the full-length Eed (embryonic ectoderm development) p...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.18.10.5634
更新日期:1998-10-01 00:00:00
abstract::We delimited sequences necessary for in vivo expression of the Drosophila melanogaster dopa decarboxylase gene Ddc. The expression of in vitro-altered genes was assayed following germ line integration via P-element vectors. Sequences between -209 and -24 were necessary for normally regulated expression, although genes...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.6.12.4548
更新日期:1986-12-01 00:00:00
abstract::Moloney murine sarcoma virus carries an oncogenic sequence (v-mos) which is homologous to a single copy gene (c-mos) present in the normal cells of several vertebrate species. Because of the possible significance of c-mos sequences in normal development and malignant transformation induced by physical or chemical agen...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.2.1.42
更新日期:1982-01-01 00:00:00
abstract::Insulin gene transcription relies on enhancer and promoter elements which are active in pancreatic beta cells. We showed that adenovirus type 5 infection of HIT T-15 cells, a transformed hamster beta cell line, represses insulin gene transcription and mRNA levels. Using expression plasmids transiently introduced into ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.7.3.1164
更新日期:1987-03-01 00:00:00
abstract::A missense mutation within the histone acetyltransferase (HAT) domain of the TATA binding protein-associated factor TAF1 induces ts13 cells to undergo a late G(1) arrest and decreases cyclin D1 transcription. We have found that TAF1 mutants (Delta844-850 and Delta848-850, from which amino acids 844 through 850 and 848...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.25.10.4321-4332.2005
更新日期:2005-05-01 00:00:00
abstract::TSLC1/IGSF4, an immunoglobulin superfamily molecule, is predominantly expressed in the brain, lungs, and testes and plays important roles in epithelial cell adhesion, cancer invasion, and synapse formation. We generated Tslc1/Igsf4-deficient mice by disrupting exon 1 of the gene and found that Tslc1(-/-) mice were bor...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.26.9.3610-3624.2006
更新日期:2006-05-01 00:00:00
abstract::Growth factor independence 1B (GFI1B) coordinates assembly of transcriptional repressor complexes comprised of corepressors and histone-modifying enzymes to control gene expression programs governing lineage allocation in hematopoiesis. Enforced expression of GFI1B in K562 erythroleukemia cells favors erythroid over m...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00020-19
更新日期:2019-06-13 00:00:00
abstract::The transcriptional activator p53 is known to interact with components of the general transcription factor TFIID in vitro. To examine the relevance of these associations to transcriptional activation in vivo, plasmids expressing a p53-GAL4 chimera and Drosophila TATA-binding protein (dTBP) were transfected into Drosop...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.16.8.4295
更新日期:1996-08-01 00:00:00
abstract::The genes that encode the variable regions of immunoglobulin (Ig) heavy chains are encoded by three DNA segments: VH, D, and JH. During B-cell development these segments are brought together by a pair of site-specific DNA rearrangements. The first of these joins a D segment to a JH segment; the second brings a VH segm...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.11.4.2096
更新日期:1991-04-01 00:00:00
abstract::Retinoid X receptors (RXR) have been identified as common subunits in the regulation of multiple hormonal signaling pathways. Using circular permutation and phasing analysis of specific response elements, we present evidence that RXR-retinoic acid receptor and RXR-thyroid hormone receptor heterodimer or RXR-RXR homodi...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.13.10.6509
更新日期:1993-10-01 00:00:00
abstract::A key antioxidant pathway, the Keap1-Nrf2 system, is regulated by p62/Sqstm1 via multiple mechanisms, including gene expression, posttranslational modifications (such as ubiquitination and phosphorylation), and autophagic degradation of p62/Sqstm1 and Keap1. Here we demonstrate a novel mode of regulation of the Keap1-...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00642-17
更新日期:2018-03-15 00:00:00
abstract::Phorbol-12-myristate-13-acetate (PMA), a potent tumor promoter, was shown to have opposite effects on the cellular morphology and steady-state levels of beta-actin mRNA in embryonic chicken muscle fibroblasts and sternal chondrocytes. When fibroblasts were treated with PMA, they formed foci of densely packed cells, ce...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.5.6.1425
更新日期:1985-06-01 00:00:00
abstract::Myocyte enhancer factor 2 (MEF2) transcription factors cooperate with the MyoD family of basic helix-loop-helix (bHLH) transcription factors to drive skeletal muscle development during embryogenesis, but little is known about the potential functions of MEF2 factors in postnatal skeletal muscle. Here we show that skele...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01187-07
更新日期:2007-12-01 00:00:00
abstract::Protein phosphatase 2A (PP2A) is a multimeric serine/threonine phosphatase that carries out multiple functions. Although numerous observations suggest that PP2A plays a major role in downregulation of the mitogen-activated protein (MAP) kinase pathway, the precise mechanisms are unknown. To clarify the role of PP2A in...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.22.7.2375-2387.2002
更新日期:2002-04-01 00:00:00
abstract::Nrdp1 is a RING finger-containing E3 ubiquitin ligase that physically interacts with and regulates steady-state cellular levels of the ErbB3 and ErbB4 receptor tyrosine kinases and has been implicated in the degradation of the inhibitor-of-apoptosis protein BRUCE. Here we demonstrate that the Nrdp1 protein undergoes e...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.24.17.7748-7757.2004
更新日期:2004-09-01 00:00:00
abstract::Cryptococcus neoformans is a fungal pathogen that causes meningitis in patients immunocompromised by AIDS, chemotherapy, organ transplantation, or high-dose steroids. Current antifungal drug therapies are limited and suffer from toxic side effects and drug resistance. Here, we defined the targets and mechanisms of ant...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.19.6.4101
更新日期:1999-06-01 00:00:00
abstract::Exposure of monocytic cells to bacterial lipopolysaccharide (LPS) activates the NF-kappa B/Rel family of proteins and leads to the rapid induction of inflammatory gene products, including tissue factor (TF). TF is the primary cellular initiator of the coagulation protease cascades. Here we report the characterization ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.14.6.3772
更新日期:1994-06-01 00:00:00
abstract::The 5' ends of dihydrofolate reductase (DHFR)-specific transcripts have been mapped in the 5'-flanking region of the amplified DHFR gene of the human methotrexate-resistant cell line 6A3 by primer extension and S1 protection experiments. The main 5' end, at position -71 relative to the first nucleotide of the DHFR rea...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.5.3.493
更新日期:1985-03-01 00:00:00
abstract::In both tissue sections and cell culture, the endothelial nature of a cell is most commonly determined by demonstration of its expression of von Willebrand factor (vWf) protein and/or mRNA. Thus, the mechanism of cell-type-specific transcriptional regulation of the vWf gene is central to studying the basis of endothel...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.14.2.999
更新日期:1994-02-01 00:00:00
abstract::The ADP/ATP translocator, a transmembrane protein of the mitochondrial inner membrane, is coded in Saccharomyces cerevisiae by the nuclear gene PET9. DNA sequence analysis of the PET9 gene showed that it encoded a protein of 309 amino acids which exhibited a high degree of homology with mitochondrial translocator prot...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.6.2.626
更新日期:1986-02-01 00:00:00
abstract::Both cyclin D1 and the transcription factor C/EBPβ are required for mammary epithelial cell differentiation; however, the pathway in which they operate is uncertain. Previous analyses of the patterns of gene expression in human tumors suggested a connection between cyclin D1 overexpression and C/EBPβ, but whether this...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00039-14
更新日期:2014-08-01 00:00:00
abstract::The chimeric oncoprotein E2A-HLF, generated by the t(17;19) chromosomal translocation in pro-B-cell acute lymphoblastic leukemia, incorporates the transactivation domains of E2A and the basic leucine zipper (bZIP) DNA-binding and protein dimerization domain of HLF (hepatic leukemic factor). The ability of E2A-HLF to p...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.18.10.6035
更新日期:1998-10-01 00:00:00
abstract::Considering the link between plasma high-density lipoprotein (HDL) cholesterol levels and a protective effect against coronary artery disease as well as the suggested beneficial effects of retinoids on the production of the major HDL apolipoprotein (apo), apo A-I, the goal of this study was to analyze the influence of...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.16.7.3350
更新日期:1996-07-01 00:00:00
abstract::Imprinted genes are expressed in a monoallelic, parent-of-origin-specific manner. Clusters of imprinted genes are regulated by imprinting control regions (ICRs) characterized by DNA methylation of one allele. This methylation is critical for imprinting; a reduction in the DNA methyltransferase DNMT1 causes a widesprea...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01278-09
更新日期:2010-08-01 00:00:00
abstract::GPR103 is a G-protein-coupled receptor with reported expression in brain, heart, kidney, adrenal gland, retina, and testis. It encodes a 455-amino-acid protein homologous to neuropeptide FF2, neuropeptide Y2, and galanin GalR1 receptors. Its natural ligand was recently identified as 26RFa, a novel human RF-amide-relat...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.26.2.709-717.2006
更新日期:2006-01-01 00:00:00
abstract::Syncollin is a small protein that is abundantly expressed in pancreatic acinar cells and that is tightly associated with the lumenal side of the zymogen granule membrane. To shed light on the hitherto unknown function of syncollin, we have generated syncollin-deficient mice. The mice are viable and show a normal pancr...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.22.5.1545-1554.2002
更新日期:2002-03-01 00:00:00
abstract::The hepatocyte nuclear factor 3alpha (HNF-3alpha) and 3beta proteins have homology in the winged helix/fork head DNA binding domain and regulate cell-specific transcription in hepatocytes and in respiratory and intestinal epithelia. In this study, we describe two novel isoforms of the winged helix transcription factor...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.17.3.1626
更新日期:1997-03-01 00:00:00
abstract::The apoptotic process is accompanied by major changes in chromatin structure and gene expression. The apoptotic genetic program is progressively set up with the inhibition of antiapoptotic genes and the activation of proapoptotic ones. Here, we show that the histone deacetylase 3 (HDAC-3), which is a known co-represso...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00869-06
更新日期:2007-01-01 00:00:00
abstract::Inactivation of the tumor suppressor p53 by degradation is a mechanism utilized by cells to adapt to endoplasmic reticulum (ER) stress. However, the mechanisms of p53 destabilization by ER stress are not known. We demonstrate here that the E3 ubiquitin-ligase Hdm2 is essential for the nucleocytoplasmic transport and p...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.25.21.9392-9405.2005
更新日期:2005-11-01 00:00:00
abstract::We examined the effects of 18 h of incubation of Chinese hamster ovary (CHO K1) cells with cycloheximide, hydroxyurea, and aphidicolin. Treatment of cells with cycloheximide alone at a concentration adequate to inhibit DNA synthesis to less than 10% of control was significantly less cytotoxic and clastogenic than trea...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.8.7.2822
更新日期:1988-07-01 00:00:00