Role for Nhp6, Gcn5, and the Swi/Snf complex in stimulating formation of the TATA-binding protein-TFIIA-DNA complex.

Abstract:

:The TATA-binding protein (TBP), TFIIA, and TFIIB interact with promoter DNA to form a complex required for transcriptional initiation, and many transcriptional regulators function by either stimulating or inhibiting formation of this complex. We have recently identified TBP mutants that are viable in wild-type cells but lethal in the absence of the Nhp6 architectural transcription factor. Here we show that many of these TBP mutants were also lethal in strains with disruptions of either GCN5, encoding the histone acetyltransferase in the SAGA complex, or SWI2, encoding the catalytic subunit of the Swi/Snf chromatin remodeling complex. These synthetic lethalities could be suppressed by overexpression of TOA1 and TOA2, the genes encoding TFIIA. We also used TFIIA mutants that eliminated in vitro interactions with TBP. These viable TFIIA mutants were lethal in strains lacking Gcn5, Swi2, or Nhp6. These lethalities could be suppressed by overexpression of TBP or Nhp6, suggesting that these coactivators stimulate formation of the TBP-TFIIA-DNA complex. In vitro studies have previously shown that TBP binds very poorly to a TATA sequence within a nucleosome but that Swi/Snf stimulates binding of TBP and TFIIA. In vitro binding experiments presented here show that histone acetylation facilitates TBP binding to a nucleosomal binding site and that Nhp6 stimulates formation of a TBP-TFIIA-DNA complex. Consistent with the idea that Nhp6, Gcn5, and Swi/Snf have overlapping functions in vivo, nhp6a nhp6b gcn5 mutants had a severe growth defect, and mutations in both nhp6a nhp6b swi2 and gcn5 swi2 strains were lethal.

journal_name

Mol Cell Biol

authors

Biswas D,Imbalzano AN,Eriksson P,Yu Y,Stillman DJ

doi

10.1128/MCB.24.18.8312-8321.2004

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

8312-21

issue

18

eissn

0270-7306

issn

1098-5549

pii

24/18/8312

journal_volume

24

pub_type

杂志文章
  • The ARID family transcription factor bright is required for both hematopoietic stem cell and B lineage development.

    abstract::Bright/Arid3a has been characterized both as an activator of immunoglobulin heavy-chain transcription and as a proto-oncogene. Although Bright expression is highly B lineage stage restricted in adult mice, its expression in the earliest identifiable hematopoietic stem cell (HSC) population suggests that Bright might h...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01448-10

    authors: Webb CF,Bryant J,Popowski M,Allred L,Kim D,Harriss J,Schmidt C,Miner CA,Rose K,Cheng HL,Griffin C,Tucker PW

    更新日期:2011-03-01 00:00:00

  • Linear distance from the locus control region determines epsilon-globin transcriptional activity.

    abstract::Enhancer elements modulate promoter activity over vast chromosomal distances, and mechanisms that ensure restrictive interactions between promoters and enhancers are critical for proper control of gene expression. The human beta-globin locus control region (LCR) activates expression of five genes in erythroid cells, i...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00602-07

    authors: Shimotsuma M,Matsuzaki H,Tanabe O,Campbell AD,Engel JD,Fukamizu A,Tanimoto K

    更新日期:2007-08-01 00:00:00

  • Snm1-deficient mice exhibit accelerated tumorigenesis and susceptibility to infection.

    abstract::The eukaryotic SNM1 gene family has been implicated in a number of cellular pathways, including repair of DNA interstrand cross-links, involvement in VDJ recombination, repair of DNA double-strand breaks, and participation in cell cycle checkpoint pathways. In particular, mammalian SNM1 has been shown to be required i...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.25.22.10071-10078.2005

    authors: Ahkter S,Richie CT,Zhang N,Behringer RR,Zhu C,Legerski RJ

    更新日期:2005-11-01 00:00:00

  • Identification of a novel isoform of the retinoic acid receptor gamma expressed in the mouse embryo.

    abstract::Retinoic acid is known to have profound effects on developmental processes. It has been implicated as a putative morphogen in the developing chick limb bud and regenerating amphibian limb blastema and has been demonstrated to have powerful teratogenic effects in mammals, including humans. Recently, three specific reti...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.10.5.2335

    authors: Giguère V,Shago M,Zirngibl R,Tate P,Rossant J,Varmuza S

    更新日期:1990-05-01 00:00:00

  • CAPNS1 regulates USP1 stability and maintenance of genome integrity.

    abstract::Calpains regulate a wide spectrum of biological functions, including migration, adhesion, apoptosis, secretion, and autophagy, through the modulating cleavage of specific substrates. Ubiquitous microcalpain (μ-calpain) and millicalpain (m-calpain) are heterodimers composed of catalytic subunits encoded, respectively, ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01406-12

    authors: Cataldo F,Peche LY,Klaric E,Brancolini C,Myers MP,Demarchi F,Schneider C

    更新日期:2013-06-01 00:00:00

  • Nuclear envelope localization of an adenovirus tumor antigen maintains the integrity of cellular DNA.

    abstract::The adenovirus early-region 1B 19,000-molecular-weight tumor antigen is required for oncogenic transformation of cells by adenovirus. We have demonstrated that this tumor antigen is located in the nuclear envelope of infected and transformed cells and that a fraction of the protein within the nuclear envelope is assoc...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.4.12.2865

    authors: White E,Blose SH,Stillman BW

    更新日期:1984-12-01 00:00:00

  • Negative regulation of transcription in vitro by a glucocorticoid response element is mediated by a trans-acting factor.

    abstract::In vitro experiments with cell extracts prepared from a mouse mammary epithelial cell line demonstrated that a cis-acting glucocorticoid response element (GRE) of the mouse mammary tumor virus represses transcription from its homologous promoter. Competition transcription experiments, in which a molar excess of a rest...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.8.9.3872

    authors: Langer SJ,Ostrowski MC

    更新日期:1988-09-01 00:00:00

  • Peroxisome proliferator-activated receptor alpha regulates a microRNA-mediated signaling cascade responsible for hepatocellular proliferation.

    abstract::Activation of peroxisome proliferator-activated receptor alpha (PPARalpha) leads to hepatocellular proliferation and liver carcinomas. The early events mediating these effects are unknown. A novel mechanism by which PPARalpha regulates gene expression and hepatocellular proliferation was uncovered. MicroRNA (miRNA) ex...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00317-07

    authors: Shah YM,Morimura K,Yang Q,Tanabe T,Takagi M,Gonzalez FJ

    更新日期:2007-06-01 00:00:00

  • I-SceI-induced gene replacement at a natural locus in embryonic stem cells.

    abstract::Gene targeting is a very powerful tool for studying mammalian development and physiology and for creating models of human diseases. In many instances, however, it is desirable to study different modifications of a target gene, but this is limited by the generally low frequency of homologous recombination in mammalian ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.18.3.1444

    authors: Cohen-Tannoudji M,Robine S,Choulika A,Pinto D,El Marjou F,Babinet C,Louvard D,Jaisser F

    更新日期:1998-03-01 00:00:00

  • Polycomb repressive complex 2 and H3K27me3 cooperate with H3K9 methylation to maintain heterochromatin protein 1α at chromatin.

    abstract::Methylation of histone H3 on lysine 9 or 27 is crucial for heterochromatin formation. Previously considered hallmarks of, respectively, constitutive and facultative heterochromatin, recent evidence has accumulated in favor of coexistence of these two marks and their cooperation in gene silencing maintenance. H3K9me2/3...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00205-14

    authors: Boros J,Arnoult N,Stroobant V,Collet JF,Decottignies A

    更新日期:2014-10-01 00:00:00

  • The T-cell oncogenic protein HOX11 activates Aldh1 expression in NIH 3T3 cells but represses its expression in mouse spleen development.

    abstract::Hox11 is a homeobox gene essential for spleen formation in mice, since atrophy of the anlage of a developing spleen occurs in early embryonic development in Hox11 null mice. HOX11 is also expressed in a subset of T-cell acute leukemias after specific chromosomal translocations. Since the protein has a homeodomain and ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.18.12.7030

    authors: Greene WK,Bahn S,Masson N,Rabbitts TH

    更新日期:1998-12-01 00:00:00

  • Function and control of RNA polymerase II C-terminal domain phosphorylation in vertebrate transcription and RNA processing.

    abstract::The C-terminal domain of the RNA polymerase II largest subunit (the Rpb1 CTD) is composed of tandem heptad repeats of the consensus sequence Y(1)S(2)P(3)T(4)S(5)P(6)S(7). We reported previously that Thr 4 is phosphorylated and functions in histone mRNA 3'-end formation in chicken DT40 cells. Here, we have extended our...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00181-14

    authors: Hsin JP,Xiang K,Manley JL

    更新日期:2014-07-01 00:00:00

  • Activation of an enhancer on the syndecan-1 gene is restricted to fibroblast growth factor family members in mesenchymal cells.

    abstract::Fibroblast growth factors (FGFs) induce a variety of biological effects on different cell types. They activate a number of genes, including immediate-early genes, such as the transcription factors Fos and Jun, which are also common targets for other tyrosine kinase receptor-activating growth factors. Here we describe ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.17.6.3210

    authors: Jaakkola P,Vihinen T,Määttä A,Jalkanen M

    更新日期:1997-06-01 00:00:00

  • Loss of YY1 impacts the heterochromatic state and meiotic double-strand breaks during mouse spermatogenesis.

    abstract::The progression of spermatogenesis involves global changes in chromatin structure and conformation. However, our understanding of the regulation of chromatin changes in germ cells remains limited. Here we describe both in vivo RNA interference and genetic mouse knockout studies that identify a critical role for Yin Ya...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00679-09

    authors: Wu S,Hu YC,Liu H,Shi Y

    更新日期:2009-12-01 00:00:00

  • A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein.

    abstract::Human La protein has been shown to serve as a transcription factor for RNA polymerase III (pol III) by facilitating transcription termination and recycling of transcription complexes. In addition, La binds to the 3' oligo(U) ends common to all nascent pol III transcripts, and in the case of B1-Alu RNA, protects it fro...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.17.10.5823

    authors: Goodier JL,Fan H,Maraia RJ

    更新日期:1997-10-01 00:00:00

  • Involvement of a NF-kappa B-like transcription factor in the activation of the interleukin-6 gene by inflammatory lymphokines.

    abstract::Interleukin-6 (IL-6) is one of the major mediators of inflammation, and its expression is inducible by the other inflammatory lymphokines, interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-alpha). We demonstrate that a common IL-6 promoter element, termed inflammatory lymphokine-responsive element (ILRE), is i...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.10.2.561

    authors: Shimizu H,Mitomo K,Watanabe T,Okamoto S,Yamamoto K

    更新日期:1990-02-01 00:00:00

  • An upstream open reading frame and the context of the two AUG codons affect the abundance of mitochondrial and nuclear RNase H1.

    abstract::RNase H1 in mammalian cells is present in nuclei and mitochondria. Its absence in mitochondria results in embryonic lethality due to the failure to amplify mitochondrial DNA (mtDNA). Dual localization to mitochondria and nuclei results from differential translation initiation at two in-frame AUGs (M1 and M27) of a sin...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00619-10

    authors: Suzuki Y,Holmes JB,Cerritelli SM,Sakhuja K,Minczuk M,Holt IJ,Crouch RJ

    更新日期:2010-11-01 00:00:00

  • Novel mRNA targets for tristetraprolin (TTP) identified by global analysis of stabilized transcripts in TTP-deficient fibroblasts.

    abstract::Tristetraprolin (TTP) is a tandem CCCH zinc finger protein that was identified through its rapid induction by mitogens in fibroblasts. Studies of TTP-deficient mice and cells derived from them showed that TTP could bind to certain AU-rich elements in mRNAs, leading to increases in the rates of mRNA deadenylation and d...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00945-06

    authors: Lai WS,Parker JS,Grissom SF,Stumpo DJ,Blackshear PJ

    更新日期:2006-12-01 00:00:00

  • Mitochondrial DNA instability and peri-implantation lethality associated with targeted disruption of nuclear respiratory factor 1 in mice.

    abstract::In vitro studies have implicated nuclear respiratory factor 1 (NRF-1) in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. We investigated here the in vivo function of NRF-1 in mammals by disrupting the gene in mice. A...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.21.2.644-654.2001

    authors: Huo L,Scarpulla RC

    更新日期:2001-01-01 00:00:00

  • Telomere maintenance through spatial control of telomeric proteins.

    abstract::The six human telomeric proteins TRF1, TRF2, RAP1, TIN2, POT1, and TPP1 can form a complex called the telosome/shelterin, which is required for telomere protection and length control. TPP1 has been shown to regulate both POT1 telomere localization and telosome assembly through its binding to TIN2. It remains to be det...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00603-07

    authors: Chen LY,Liu D,Songyang Z

    更新日期:2007-08-01 00:00:00

  • Surface expression of influenza virus neuraminidase, an amino-terminally anchored viral membrane glycoprotein, in polarized epithelial cells.

    abstract::We have investigated the site of surface expression of the neuraminidase (NA) glycoprotein of influenza A virus, which, in contrast to the hemagglutinin, is bound to membranes by hydrophobic residues near the NH2-terminus. Madin-Darby canine kidney or primary African green monkey kidney cells infected with influenza A...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.5.9.2181

    authors: Jones LV,Compans RW,Davis AR,Bos TJ,Nayak DP

    更新日期:1985-09-01 00:00:00

  • Interleukin-5 (IL-5) and IL-6 define two molecularly distinct pathways of B-cell differentiation.

    abstract::Interleukin-5 (IL-5) and IL-6 have both been reported to act as B-cell differentiation factors by stimulating activated B cells to secrete antibody. However, it has not been possible to directly compare the effects of these two lymphokines because of the lack of a suitable B-cell line capable of responding to both. We...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.13.7.3929

    authors: Randall TD,Lund FE,Brewer JW,Aldridge C,Wall R,Corley RB

    更新日期:1993-07-01 00:00:00

  • The Saccharomyces cerevisiae SPT13/GAL11 gene has both positive and negative regulatory roles in transcription.

    abstract::To understand the function of SPT13, a gene encoding a trans-acting factor involved in regulation of Ty-mediated gene expression, we have cloned and sequenced the gene. Our analysis revealed that SPT13 is the same gene as GAL11, a gene in which mutations cause reduced expression of some GAL4-regulated genes. Further a...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.9.12.5602

    authors: Fassler JS,Winston F

    更新日期:1989-12-01 00:00:00

  • Tenomodulin is necessary for tenocyte proliferation and tendon maturation.

    abstract::Tenomodulin (Tnmd) is a member of a new family of type II transmembrane glycoproteins. It is predominantly expressed in tendons, ligaments, and eyes, whereas the only other family member, chondromodulin I (ChM-I), is highly expressed in cartilage and at lower levels in the eye and thymus. The C-terminal extracellular ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.25.2.699-705.2005

    authors: Docheva D,Hunziker EB,Fässler R,Brandau O

    更新日期:2005-01-01 00:00:00

  • Okadaic acid induces selective arrest of protein transport in the rough endoplasmic reticulum and prevents export into COPII-coated structures.

    abstract::Quantitative immunoelectron microscopy and subcellular fractionation established the site of endoplasmic reticulum (ER)-Golgi transport arrest induced by the phosphatase inhibitor okadaic acid (OA). OA induced the disappearance of transitional element tubules and accumulation of the anterograde-transported Chandipura ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.18.2.1125

    authors: Pryde JG,Farmaki T,Lucocq JM

    更新日期:1998-02-01 00:00:00

  • Endothelial-cell-specific regulation of von Willebrand factor gene expression.

    abstract::In both tissue sections and cell culture, the endothelial nature of a cell is most commonly determined by demonstration of its expression of von Willebrand factor (vWf) protein and/or mRNA. Thus, the mechanism of cell-type-specific transcriptional regulation of the vWf gene is central to studying the basis of endothel...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.14.2.999

    authors: Jahroudi N,Lynch DC

    更新日期:1994-02-01 00:00:00

  • Homeodomain-interacting protein kinase 1 modulates Daxx localization, phosphorylation, and transcriptional activity.

    abstract::We describe an interaction between homeodomain-interacting protein kinase 1 (HIPK1) and Daxx, two transcriptional regulators important in transducing growth-regulatory signals. We demonstrate that HIPK1 is ubiquitously expressed in mice and humans and localizes predominantly to the nucleus. Daxx normally resides withi...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.23.3.950-960.2003

    authors: Ecsedy JA,Michaelson JS,Leder P

    更新日期:2003-02-01 00:00:00

  • Association of Rad9 with double-strand breaks through a Mec1-dependent mechanism.

    abstract::Rad9 is required for the activation of DNA damage checkpoint pathways in budding yeast. Rad9 is phosphorylated after DNA damage in a Mec1- and Tel1-dependent manner and subsequently interacts with Rad53. This Rad9-Rad53 interaction has been suggested to trigger the activation and phosphorylation of Rad53. Here we show...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.24.8.3277-3285.2004

    authors: Naiki T,Wakayama T,Nakada D,Matsumoto K,Sugimoto K

    更新日期:2004-04-01 00:00:00

  • The Caenorhabditis elegans EGL-15 signaling pathway implicates a DOS-like multisubstrate adaptor protein in fibroblast growth factor signal transduction.

    abstract::EGL-15 is a fibroblast growth factor receptor in the nematode Caenorhabditis elegans. Components that mediate EGL-15 signaling have been identified via mutations that confer a Clear (Clr) phenotype, indicative of hyperactivity of this pathway, or a suppressor-of-Clr (Soc) phenotype, indicative of reduced pathway activ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.21.23.8104-8116.2001

    authors: Schutzman JL,Borland CZ,Newman JC,Robinson MK,Kokel M,Stern MJ

    更新日期:2001-12-01 00:00:00

  • The capable ABL: what is its biological function?

    abstract::The mammalian ABL1 gene encodes the ubiquitously expressed nonreceptor tyrosine kinase ABL. In response to growth factors, cytokines, cell adhesion, DNA damage, oxidative stress, and other signals, ABL is activated to stimulate cell proliferation or differentiation, survival or death, retraction, or migration. ABL als...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章,评审

    doi:10.1128/MCB.01454-13

    authors: Wang JY

    更新日期:2014-04-01 00:00:00