DNA-binding-defective mutants of the Epstein-Barr virus lytic switch activator Zta transactivate with altered specificities.

abstract：:The protein components that direct and activate accurate transcription by rat RNA polymerase I were studied in extracts of Novikoff hepatoma ascites cells. A minimum of at least two components, besides RNA polymerase I, that are necessary for efficient utilization of templates were identified. The first factor, rat SL...

journal_title：Molecular and cellular biology

authors： Smith SD,Oriahi E,Lowe D,Yang-Yen HF,O'Mahony D,Rose K,Chen K,Rothblum LI

更新日期：1990-06-01 00:00:00

abstract：:We have investigated the in vivo and in vitro regulation of the human urokinase-type plasminogen activator (uPA) gene by interleukin-1 (IL-1) and analyzed the transcription factors and signalling pathways involved in the response of the -2.0-kb uPA enhancer to IL-1 induction and to tetradecanoyl phorbol acetate (TPA) ...

journal_title：Molecular and cellular biology

authors： Cirillo G,Casalino L,Vallone D,Caracciolo A,De Cesare D,Verde P

更新日期：1999-09-01 00:00:00

abstract：:The serine/threonine kinase Akt is a component of many receptor signal transduction pathways and can prevent cell death following growth factor withdrawal. Here, we show that Akt inhibition of cell death is not dependent on new protein translation. Instead, Akt inhibition of cell death requires glucose hydrolysis thro...

journal_title：Molecular and cellular biology

authors： Rathmell JC,Fox CJ,Plas DR,Hammerman PS,Cinalli RM,Thompson CB

更新日期：2003-10-01 00:00:00

abstract：:The selective expression of a unique copy gene in several mammalian tissues has been approached by studying the regulatory sequences needed to control expression of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene in transgenic mice. A transgene containing the entire PEPCK gene, including 2.2 kb of the 5'-flanki...

journal_title：Molecular and cellular biology

authors： Eisenberger CL,Nechushtan H,Cohen H,Shani M,Reshef L

更新日期：1992-03-01 00:00:00

abstract：:The Eph receptors are the largest known family of receptor tyrosine kinases and are notable for distinctive expression patterns in the nervous system and in early vertebrate development. However, all were identified as orphan receptors, and only recently have there been descriptions of a corresponding family of ligand...

journal_title：Molecular and cellular biology

authors： Bergemann AD,Cheng HJ,Brambilla R,Klein R,Flanagan JG

更新日期：1995-09-01 00:00:00

abstract：:The human T-cell leukemia virus-encoded oncoprotein Tax is a potent activator of viral transcription. Tax function is strictly dependent upon the cellular transcription factor CREB, and together they bind cAMP response elements within the viral promoter and mediate high-level viral transcription. Signal-dependent CREB...

journal_title：Molecular and cellular biology

authors： Geiger TR,Sharma N,Kim YM,Nyborg JK

更新日期：2008-02-01 00:00:00

abstract：:We set up an alternative splicing system in vitro in which the relative amounts of two spliced RNAs, one containing and the other lacking a particular exon, were directly proportional to the length of an inverted repeat inserted into the flanking introns. We then used the system to measure the effect of intramolecular...

journal_title：Molecular and cellular biology

authors： Solnick D,Lee SI

更新日期：1987-09-01 00:00:00

abstract：:The fucose alpha(1-->2) galactose beta structure is expressed by uterine epithelial cells in the mouse and has been implicated in blastocyst adhesion events thought to be required for murine implantation. Fucalpha(1-->2)Galbeta moieties and cognate fucosyltransferases are also expressed by epithelial cells of the male...

journal_title：Molecular and cellular biology

authors： Domino SE,Zhang L,Gillespie PJ,Saunders TL,Lowe JB

更新日期：2001-12-01 00:00:00

abstract：:Cyclic AMP-responsive element-binding protein 3-like 3, hepatocyte specific (CREBH), is a hepatic transcription factor that functions as a key regulator of energy homeostasis. Here, we defined a regulatory CREBH posttranslational modification process, namely, lysine-specific acetylation, and its functional involvement...

journal_title：Molecular and cellular biology

authors： Kim H,Mendez R,Chen X,Fang D,Zhang K

更新日期：2015-12-01 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (RB) is a negative regulator of the cell cycle that inhibits both G(1) and S-phase progression. While RB-mediated G(1) inhibition has been extensively studied, the mechanism utilized for S-phase inhibition is unknown. To delineate the mechanism through which RB inhibits DNA ...

journal_title：Molecular and cellular biology

authors： Sever-Chroneos Z,Angus SP,Fribourg AF,Wan H,Todorov I,Knudsen KE,Knudsen ES

更新日期：2001-06-01 00:00:00

abstract：:During mammalian vascular development, endothelial cells form a complex array of vessels that differ markedly in structure and function, but the molecular basis for this vascular complexity is poorly understood. Recent insights into endothelial diversity have come from the identification of molecular markers expressed...

journal_title：Molecular and cellular biology

authors： Xu B,deWaal RM,Mor-Vaknin N,Hibbard C,Markovitz DM,Kahn ML

更新日期：2004-10-01 00:00:00

abstract：:Src homology 2 (SH2) domains provide specificity to intracellular signaling by binding to specific phosphotyrosine (phospho-Tyr)-containing sequences. We recently developed a technique using a degenerate phosphopeptide library to predict the specificity of individual SH2 domains (src family members, Abl, Nck, Sem5, ph...

journal_title：Molecular and cellular biology

authors： Songyang Z,Shoelson SE,McGlade J,Olivier P,Pawson T,Bustelo XR,Barbacid M,Sabe H,Hanafusa H,Yi T

更新日期：1994-04-01 00:00:00

abstract：:Axin is a recently identified protein encoded by the fused locus in mice that is required for normal vertebrate axis formation. We have defined a 25-amino-acid sequence in axin that comprises the glycogen synthase kinase 3beta (GSK-3beta) interaction domain (GID). In contrast to full-length axin, which has been shown ...

journal_title：Molecular and cellular biology

authors： Hedgepeth CM,Deardorff MA,Rankin K,Klein PS

更新日期：1999-10-01 00:00:00

abstract：:We identified Ras guanine-releasing protein 3 (RasGRP3) as a guanine exchange factor expressed in blood vessels via an embryonic stem (ES) cell-based gene trap screen to identify novel vascular genes. RasGRP3 is expressed in embryonic blood vessels, down-regulated in mature adult vessels, and reexpressed in newly form...

journal_title：Molecular and cellular biology

authors： Roberts DM,Anderson AL,Hidaka M,Swetenburg RL,Patterson C,Stanford WL,Bautch VL

更新日期：2004-12-01 00:00:00

abstract：:The cardiac troponin C (cTnC) gene produces identical transcripts in slow-twitch skeletal muscle and in heart muscle (R. Gahlmann, R. Wade, P. Gunning, and L. Kedes, J. Mol. Biol. 201:379-391, 1988). A separate gene encodes the fast-twitch skeletal muscle troponin C and is not expressed in heart muscle. We have used t...

journal_title：Molecular and cellular biology

authors： Christensen TH,Prentice H,Gahlmann R,Kedes L

更新日期：1993-11-01 00:00:00

abstract：:The 5' ends of dihydrofolate reductase (DHFR)-specific transcripts have been mapped in the 5'-flanking region of the amplified DHFR gene of the human methotrexate-resistant cell line 6A3 by primer extension and S1 protection experiments. The main 5' end, at position -71 relative to the first nucleotide of the DHFR rea...

journal_title：Molecular and cellular biology

authors： Masters JN,Attardi G

更新日期：1985-03-01 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcription factor that can be activated by structurally diverse chemicals. To examine the mechanisms responsible for the promiscuity in AhR ligand binding, we determined the effects of mutations within the AhR ligand-binding domain (LBD) on the activity of d...

journal_title：Molecular and cellular biology

authors： Soshilov AA,Denison MS

更新日期：2014-05-01 00:00:00

abstract：:Hepatocyte nuclear factor 4α (HNF4α) is a critical factor for hepatocyte differentiation. HNF4α expression is decreased in hepatocellular carcinoma (HCC), which suggests a role in repression of hepatocyte dedifferentiation. In the present study, hepatic expression of HNF4γ was increased in liver-specific Hnf4a-null mi...

journal_title：Molecular and cellular biology

authors： Sasaki S,Urabe M,Maeda T,Suzuki J,Irie R,Suzuki M,Tomaru Y,Sakaguchi M,Gonzalez FJ,Inoue Y

更新日期：2018-11-28 00:00:00

abstract：:The two beta-tubulin genes of the unicellular green alga Chlamydomonas reinhardtii are expressed coordinately after deflagellation and produce two transcripts of 2.1 and 2.0 kilobases. Full-length cDNA clones corresponding to the transcript of each gene were isolated. DNA sequences were obtained from the cDNA clones a...

journal_title：Molecular and cellular biology

authors： Youngblom J,Schloss JA,Silflow CD

更新日期：1984-12-01 00:00:00

abstract：:Fusions between the TRM1 gene of Saccharomyces cerevisiae and COXIV or DHFR were made to examine the mitochondrial targeting signals of N2,N2-dimethylguanosine-specific tRNA methyltransferase [tRNA (m2(2)G)dimethyltransferase]. This enzyme is responsible for the modification of both mitochondrial and cytoplasmic tRNAs...

journal_title：Molecular and cellular biology

authors： Ellis SR,Hopper AK,Martin NC

更新日期：1989-04-01 00:00:00

abstract：:Deltex1, Deltex2, and Deltex4 form a family of related proteins that are the mammalian homologues of Drosophila Deltex, a known regulator of Notch signals. Deltex1 is highly induced by Notch signaling in thymocytes, and overexpression of Deltex1 in T-cell progenitors can block Notch signals, suggesting that Deltex1 ma...

journal_title：Molecular and cellular biology

authors： Lehar SM,Bevan MJ

更新日期：2006-10-01 00:00:00

abstract：:Entry into mitosis depends upon activation of the dual-specificity phosphatase Cdc25C, which dephosphorylates and activates the cyclin B-Cdc2 complex. Previous work has shown that the Xenopus polo-like kinase Plx1 can phosphorylate and activate Cdc25C in vitro. In the work presented here, we demonstrate that Plx1 is a...

journal_title：Molecular and cellular biology

authors： Qian YW,Erikson E,Li C,Maller JL

更新日期：1998-07-01 00:00:00

abstract：:The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B). In most cell contexts, hPR-B functions as a transcriptional activator of progesterone-responsive genes, whereas hPR-A functions as a transcriptional inhibitor of all steroid hormone receptors...

journal_title：Molecular and cellular biology

authors： Wen DX,Xu YF,Mais DE,Goldman ME,McDonnell DP

更新日期：1994-12-01 00:00:00

abstract：:Eucaryotic initiation factor 4A (eIF-4A) is a member of a family of proteins believed to be involved in the ATP-dependent melting of RNA secondary structure. These proteins contain a derivative of the consensus ATP-binding site AXXGXGKT. To assess the importance of the consensus amino acid sequence in eIF-4A for ATP b...

journal_title：Molecular and cellular biology

authors： Rozen F,Pelletier J,Trachsel H,Sonenberg N

更新日期：1989-09-01 00:00:00

abstract：:Mutant strain d48 of Paramecium tetraurelia lacks the gene for antigen A in the macronucleus, whereas this gene is present in the micronucleus. Transfer of macronucleoplasm from the wild type to strain d48 caused d48 to revert to the wild type after autogamy. Transfer of cytoplasm was not as effective as transfer of m...

journal_title：Molecular and cellular biology

authors： Harumoto T

更新日期：1986-10-01 00:00:00

abstract：:Chinese hamster-human interspecific hybrid cells, which contain human chromosome 5 and express four genes linked on that chromosome, were subjected to selective conditions requiring them to retain one of the four linked genes, leuS (encoding leucyl-tRNA synthetase), but lose another, either emtB (encoding ribosomal pr...

journal_title：Molecular and cellular biology

authors： Dana S,Wasmuth JJ

更新日期：1982-10-01 00:00:00

abstract：:Determining the functions of novel genes implicated in cell survival is directly relevant to our understanding of mammalian development and carcinogenesis. ARS2 is an evolutionarily conserved gene that confers arsenite resistance on arsenite-sensitive Chinese hamster ovary cells. Little is known regarding the function...

journal_title：Molecular and cellular biology

authors： Wilson MD,Wang D,Wagner R,Breyssens H,Gertsenstein M,Lobe C,Lu X,Nagy A,Burke RD,Koop BF,Howard PL

更新日期：2008-03-01 00:00:00

abstract：:Krüppel-like factors 3 and 8 (KLF3 and KLF8) are highly related transcriptional regulators that bind to similar sequences of DNA. We have previously shown that in erythroid cells there is a regulatory hierarchy within the KLF family, whereby KLF1 drives the expression of both the Klf3 and Klf8 genes and KLF3 in turn r...

journal_title：Molecular and cellular biology

authors： Funnell AP,Mak KS,Twine NA,Pelka GJ,Norton LJ,Radziewic T,Power M,Wilkins MR,Bell-Anderson KS,Fraser ST,Perkins AC,Tam PP,Pearson RC,Crossley M

更新日期：2013-08-01 00:00:00

abstract：:Aurora A is a mitotic kinase that localizes to centrosomes. Expression of this protein is normally limited to the mitotic stage (G(2)-M) of the cell cycle, whereas human cancer cells frequently exhibit overexpression of Aurora A protein regardless of the cell cycle stage. In the present study, Aurora A transgenic mous...

journal_title：Molecular and cellular biology

authors： Fukuda T,Mishina Y,Walker MP,DiAugustine RP

更新日期：2005-06-01 00:00:00

abstract：:To characterize further the regulatory mechanism modulating the expression of the Saccharomyces cerevisiae ARG3 gene, i.e., the specific repression by arginine and the general amino acid control, we analyzed by deletion the region upstream of that gene, determined the nucleotide sequence of operator-constitutive-like ...

journal_title：Molecular and cellular biology

authors： Crabeel M,Huygen R,Verschueren K,Messenguy F,Tinel K,Cunin R,Glansdorff N

更新日期：1985-11-01 00:00:00