Characterization of factors that direct transcription of rat ribosomal DNA.

abstract：:Posttranscriptional and posttranslational modification of macromolecules is known to fine-tune their functions. Trm112 is unique, acting as an activator of both tRNA and protein methyltransferases. Here we report that in Saccharomyces cerevisiae, Trm112 is required for efficient ribosome synthesis and progression thro...

journal_title：Molecular and cellular biology

authors： Figaro S,Wacheul L,Schillewaert S,Graille M,Huvelle E,Mongeard R,Zorbas C,Lafontaine DL,Heurgué-Hamard V

更新日期：2012-06-01 00:00:00

abstract：:We have developed a high-frequency cotransformation system which is useful in introducing nonreplicating circular DNA plasmids into the fission yeast Schizosaccharomyces pombe. This system depends on two factors: the ability of the ural-complementing helper plasmids pFYM2 and pFYM225 to propagate autonomously in S. po...

journal_title：Molecular and cellular biology

authors： Sakai K,Sakaguchi J,Yamamoto M

更新日期：1984-04-01 00:00:00

abstract：:Cell polarity plays a critical role in the development of all metazoans; however, the mechanisms of cell polarity and the specific role of cell polarity pathways in mammalian organisms are still poorly understood. Lethal giant larvae (Lgl) is an apical-basal polarity gene identified in Drosophila, where it functions a...

journal_title：Molecular and cellular biology

authors： Sripathy S,Lee M,Vasioukhin V

更新日期：2011-07-01 00:00:00

abstract：:Barsoum and Varshavsky (Proc. Natl. Acad. Sci. U.S.A. 80:5330-5334, 1983) suggest that polypeptide mitogens and the mitogenic tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulate gene amplification by related pathways. I demonstrated that TPA and the polypeptide mitogen fibroblast growth factor (FGF) bo...

journal_title：Molecular and cellular biology

authors： Herschman HR

更新日期：1985-05-01 00:00:00

abstract：:In this report, we explored the mechanisms underlying keratinocyte-specific and differentiation-specific gene expression in the skin. We have identified five keratinocyte-specific, open chromatin regions that exist within the 6 kb of 5' upstream regulatory sequence known to faithfully recapitulate the strong endogenou...

journal_title：Molecular and cellular biology

authors： Kaufman CK,Sinha S,Bolotin D,Fan J,Fuchs E

更新日期：2002-06-01 00:00:00

abstract：:We analyzed the binding site(s) for Grb2 on the epidermal growth factor (EGF) receptor (EGFR), using cell lines overexpressing EGFRs containing various point and deletion mutations in the carboxy-terminal tail. Results of co-immunoprecipitation experiments suggest that phosphotyrosines Y-1068 and Y-1173 mediate the bi...

journal_title：Molecular and cellular biology

authors： Batzer AG,Rotin D,Ureña JM,Skolnik EY,Schlessinger J

更新日期：1994-08-01 00:00:00

abstract：:The nuclear factor of activated T cells (NFAT) regulates cytokine gene expression in T cells through cis-acting elements located in the promoters of cytokine genes. Here, we report the cDNA cloning, chromosomal localization, and initial characterization of a transcription factor related to NFATp and NFATc. The novel m...

journal_title：Molecular and cellular biology

authors： Masuda ES,Naito Y,Tokumitsu H,Campbell D,Saito F,Hannum C,Arai K,Arai N

更新日期：1995-05-01 00:00:00

abstract：:Hepatitis C virus (HCV) NS5B protein is a membrane-associated phosphoprotein that possesses an RNA-dependent RNA polymerase activity. We recently reported that NS5A protein interacts with TRAF2 and modulates tumor necrosis factor alpha (TNF-alpha)-induced NF-kappaB and Jun N-terminal protein kinase (JNK). Since NS5A a...

journal_title：Molecular and cellular biology

authors： Choi SH,Park KJ,Ahn BY,Jung G,Lai MM,Hwang SB

更新日期：2006-04-01 00:00:00

abstract：:We previously purified and characterized functionally the Escherichia coli-expressed product of the human subgroup C adenovirus E1A 13S mRNA (B. Ferguson, N. Jones, J. Richter, and M. Rosenberg, Science 224:1343-1346, 1984; B. Krippl, B. Ferguson, M. Rosenberg, and H. Westphal, Proc. Natl. Acad. Sci. USA 81:6988-6992,...

journal_title：Molecular and cellular biology

authors： Ferguson B,Krippl B,Andrisani O,Jones N,Westphal H,Rosenberg M

更新日期：1985-10-01 00:00:00

abstract：:The HRX gene (also called MLL, ALL-1, and Htrx) at chromosome band 11q23 is associated with specific subsets of acute leukemias through translocations that result in its fusion with a variety of heterologous partners. Two of these partners, ENL and AF9, code for proteins that are highly similar to each other and as fu...

journal_title：Molecular and cellular biology

authors： Slany RK,Lavau C,Cleary ML

更新日期：1998-01-01 00:00:00

abstract：:Cell numbers are regulated by a balance among proliferation, growth arrest, and programmed cell death. A profound example of cell homeostasis, controlled throughout life, is the complex process of blood cell development, yet little is understood about the intracellular mechanisms that regulate blood cell growth arrest...

journal_title：Molecular and cellular biology

authors： Selvakumaran M,Lin HK,Sjin RT,Reed JC,Liebermann DA,Hoffman B

更新日期：1994-04-01 00:00:00

abstract：:Proteins encoded by the mdm2 gene, which has a pivotal role in the regulation of growth and differentiation, exist principally in human and murine cells as two isoforms that migrate in gels as 75-kDa and 90-kDa proteins. There is limited understanding of the respective biological roles of these isoforms, their molecul...

journal_title：Molecular and cellular biology

authors： Cheng TH,Cohen SN

更新日期：2007-01-01 00:00:00

abstract：:The human fetal G gamma-globin and adult beta-globin genes are expressed in a tissue- and developmental stage-specific pattern in transgenic mice: the G gamma gene in embryonic cells and the beta gene in fetal and adult erythroid cells. Several of the cis-acting DNA sequences thought to be responsible for these patter...

journal_title：Molecular and cellular biology

authors： Perez-Stable C,Costantini F

更新日期：1990-03-01 00:00:00

abstract：:Previous studies have identified novel modifications in the core fold domain of histone H2B, but relatively little is known about the function of these putative histone modification sites. We have mutated core modifiable residues that are conserved in Saccharomyces cerevisiae histone H2B and characterized the effects ...

journal_title：Molecular and cellular biology

authors： Kyriss MN,Jin Y,Gallegos IJ,Sanford JA,Wyrick JJ

更新日期：2010-07-01 00:00:00

abstract：:The Rb protein is known to exert its activity at decision points in the G1 phase of the cell cycle. To investigate whether it may also play some role(s) at later points in the cell cycle, we used a system of rapid inducible gene amplification to conditionally overexpress Rb protein during G2 phase. A cell line express...

journal_title：Molecular and cellular biology

authors： Karantza V,Maroo A,Fay D,Sedivy JM

更新日期：1993-11-01 00:00:00

abstract：:Glucocorticoid production in the adrenal cortex is activated in response to an increase in cyclic AMP (cAMP) signaling. The nuclear protein p54(nrb)/NONO belongs to the Drosophila behavior/human splicing (DBHS) family and has been implicated in several nuclear processes, including transcription, splicing, and RNA expo...

journal_title：Molecular and cellular biology

authors： Lu JY,Sewer MB

更新日期：2015-04-01 00:00:00

abstract：:The LAC9 protein of Kluyveromyces lactis is a transcriptional regulator of genes in the lactose-galactose regulon. To regulate transcription, LAC9 must bind to 17-bp upstream activator sequences (UASs) located in front of each target gene. LAC9 is homologous to the GAL4 protein of Saccharomyces cerevisiae, and the two...

journal_title：Molecular and cellular biology

authors： Halvorsen YD,Nandabalan K,Dickson RC

更新日期：1991-04-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the CDC25 gene product activates adenylate cyclase through RAS1 and RAS2 gene products. We have recently described the cloning of a DNA fragment which suppresses the cdc25 mutation but not ras1, ras2, or cdc35 mutations. This fragment contains a 5'-truncated open reading frame wh...

journal_title：Molecular and cellular biology

authors： Damak F,Boy-Marcotte E,Le-Roscouet D,Guilbaud R,Jacquet M

更新日期：1991-01-01 00:00:00

abstract：:The metabolic lifetime of mRNA can be specified by specific cis-acting elements within mRNA. One type of element is an adenylate- and uridylate-rich element (ARE) found in the 3' untranslated region of many highly unstable mRNAs for mammalian early-response genes (ERGs). Among the better-characterized members of the E...

journal_title：Molecular and cellular biology

authors： Chen CY,Shyu AB

更新日期：1994-12-01 00:00:00

abstract：:The immunoglobulin M heavy-chain locus contains two poly(A) sites which are alternatively expressed during B-cell differentiation. Despite its promoter proximal location, the secretory poly(A) site is not expressed in undifferentiated cells. Crucial to the activation of the secretory poly(A) site during B-cell differe...

journal_title：Molecular and cellular biology

authors： Phillips C,Pachikara N,Gunderson SI

更新日期：2004-07-01 00:00:00

abstract：:We have analyzed the fine structure of DNA repair in Chinese hamster ovary (CHO) cells within the G1 and G2 phases of the cell cycle. Repair of inactive regions of the genome has been suggested to increase in the G2 phase of the cell cycle compared with other phases. However, detailed studies of DNA repair in the G2 p...

journal_title：Molecular and cellular biology

authors： Petersen LN,Orren DK,Bohr VA

更新日期：1995-07-01 00:00:00

abstract：:The stem-loop in the 5' untranslated region (UTR) of collagen α1(I) and α2(I) mRNAs (5'SL) is the key element regulating their stability and translation. Stabilization of collagen mRNAs is the predominant mechanism for high collagen expression in fibrosis. LARP6 binds the 5'SL of α1(I) and α2(I) mRNAs with high affini...

journal_title：Molecular and cellular biology

authors： Challa AA,Stefanovic B

更新日期：2011-09-01 00:00:00

abstract：:DNA from the centromere region of linkage group (LG) VII of Neurospora crassa was cloned previously from a yeast artificial chromosome library and was found to be atypical of Neurospora DNA in both composition (AT rich) and complexity (repetitive). We have determined the DNA sequence of a small portion (approximately ...

journal_title：Molecular and cellular biology

authors： Cambareri EB,Aisner R,Carbon J

更新日期：1998-09-01 00:00:00

abstract：:Expression of the acute-phase response genes, such as that for alpha-1 acid glycoprotein (AGP), involves both positive and negative transcription factors. A positive transcription factor, AGP/EBP, and a negative transcription factor, factor B, have been identified as the two most important factors responsible for the ...

journal_title：Molecular and cellular biology

authors： Yang TH,Tsai WH,Lee YM,Lei HY,Lai MY,Chen DS,Yeh NH,Lee SC

更新日期：1994-09-01 00:00:00

abstract：:In both tissue sections and cell culture, the endothelial nature of a cell is most commonly determined by demonstration of its expression of von Willebrand factor (vWf) protein and/or mRNA. Thus, the mechanism of cell-type-specific transcriptional regulation of the vWf gene is central to studying the basis of endothel...

journal_title：Molecular and cellular biology

authors： Jahroudi N,Lynch DC

更新日期：1994-02-01 00:00:00

abstract：:Processing from pre-mRNA introns is a widespread mechanism to generate human box C/D and H/ACA snoRNAs. Recent studies revealed that an optimal position relative to the 3' splice site is important for efficient processing of most box C/D snoRNAs and that assembly of box C/D snoRNPs is stimulated by splicing factors li...

journal_title：Molecular and cellular biology

authors： Richard P,Kiss AM,Darzacq X,Kiss T

更新日期：2006-04-01 00:00:00

abstract：:Etoposide, a nonintercalating antitumor drug, is a potent inhibitor of topoisomerase II activity. When Trypanosoma equiperdum is treated with etoposide, cleavable complexes are stabilized between topoisomerase II and kinetoplast DNA minicircles, a component of trypanosome mitochondrial DNA (T. A. Shapiro, V. A. Klein,...

journal_title：Molecular and cellular biology

authors： Shapiro TA

更新日期：1994-06-01 00:00:00

abstract：:In vertebrates, the basic helix-loop-helix (bHLH) protein Twist may be involved in the negative regulation of cellular determination and in the differentiation of several lineages, including myogenesis, osteogenesis, and neurogenesis. Although it has been shown that mouse twist (M-Twist) (i) sequesters E proteins, thu...

journal_title：Molecular and cellular biology

authors： Hamamori Y,Wu HY,Sartorelli V,Kedes L

更新日期：1997-11-01 00:00:00

abstract：:CD133, a widely known marker of cancer stem cells, was recently found in extracellular vesicles. However, the mechanisms underlying CD133 translocation to the extracellular space remain largely unknown. Here we report that CD133 is monoubiquitinated. Ubiquitination occurs primarily on complex glycosylated CD133. The l...

journal_title：Molecular and cellular biology

authors： Yang F,Xing Y,Li Y,Chen X,Jiang J,Ai Z,Wei Y

更新日期：2018-07-16 00:00:00

abstract：:Retinoblastoma (RB) tumors develop when both alleles of a gene (RB1) are mutated and unable to function normally. Recently, Friend et al. [S. H. Friend, R. Bernards, S. Rogelj, R. A. Weinberg, J. M. Rapaport, D. M. Albert, and T. P. Dryja, Nature (London) 32:643-646, 1986] reported the cloning of a gene, 4.7R, with so...

journal_title：Molecular and cellular biology

authors： Goddard AD,Balakier H,Canton M,Dunn J,Squire J,Reyes E,Becker A,Phillips RA,Gallie BL

更新日期：1988-05-01 00:00:00