Abstract:
:In the yeast Saccharomyces cerevisiae, yeast RAS proteins are potent activators of adenylate cyclase. In the present work we measured the activity of adenylate cyclase in membranes from Saccharomyces cerevisiae which overexpress this enzyme. The response of the enzyme to added RAS2 proteins bound with various guanine nucleotides and their analogs suggests that RAS2 proteins are active in their GTP-bound form and are virtually inactive in their GDP-bound form. Also, active RAS2 protein is not inhibited by inactive RAS2, suggesting that the inactive form does not compete with the active form in binding to its effector.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Field J,Broek D,Kataoka T,Wigler Mdoi
10.1128/mcb.7.6.2128subject
Has Abstractpub_date
1987-06-01 00:00:00pages
2128-33issue
6eissn
0270-7306issn
1098-5549journal_volume
7pub_type
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