Transformation by v-myb correlates with trans-activation of gene expression.

Abstract:

:The v-myb oncogene of avian myeloblastosis virus causes acute myelomonocytic leukemia in chickens and transforms avian myeloid cells in vitro. Its protein product p48v-myb is a nuclear, sequence-specific, DNA-binding protein which activates gene expression in transient DNA transfection studies. To investigate the relationship between transformation and trans-activation by v-myb, we constructed 15 in-frame linker insertion mutants. The 12 mutants which transformed myeloid cells also trans-activated gene expression, whereas the 3 mutants which did not transform also did not trans-activate. This implies that trans-activation is required for transformation by v-myb. One of the transformation-defective mutants localized to the cell nucleus but failed to bind DNA. The other two transformation-defective mutants localized to the cell nucleus and bound DNA but nevertheless failed to trans-activate. These latter mutants define two distinct domains of p48v-myb which control trans-activation by DNA-bound protein, one within the amino-terminal DNA-binding domain itself and one in a carboxyl-terminal domain which is not required for DNA binding.

journal_name

Mol Cell Biol

authors

Lane T,Ibanez C,Garcia A,Graf T,Lipsick J

doi

10.1128/mcb.10.6.2591

subject

Has Abstract

pub_date

1990-06-01 00:00:00

pages

2591-8

issue

6

eissn

0270-7306

issn

1098-5549

journal_volume

10

pub_type

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