The Drosophila poly(A) binding protein-interacting protein, dPaip2, is a novel effector of cell growth.

abstract：:Expression of the reaper gene (rpr) correlates with the initiation of apoptosis in Drosophila melanogaster. Transient expression of rpr in the lepidopteran SF-21 cell line induced apoptosis displaying nuclear condensation and fragmentation, oligonucleosomal ladder formation, cell surface blebbing, and apoptotic body f...

journal_title：Molecular and cellular biology

authors： Vucic D,Seshagiri S,Miller LK

更新日期：1997-02-01 00:00:00

abstract：:We have isolated new histone H3 mutants in Saccharomyces cerevisiae that confer phenotypes indicative of transcriptional defects. Here we describe the characterization of one such mutant, encoded by the hht2-11 allele, which contains the single amino acid change L61W in the globular domain of H3. Whole-genome expressi...

journal_title：Molecular and cellular biology

authors： Duina AA,Winston F

更新日期：2004-01-01 00:00:00

abstract：:Acidic fibroblast growth factor (FGF) and related family members regulate differentiation in organisms as diverse as Xenopus laevis and mammals. We utilized a well-characterized model of myogenic development to directly assess the importance of endogenously produced FGF in controlling differentiation. A role for endog...

journal_title：Molecular and cellular biology

authors： Fox JC,Hsu AY,Swain JL

更新日期：1994-06-01 00:00:00

abstract：:DHCR24/seladin-1, a crucial enzyme in sterol synthesis, is of lower abundance in brain areas affected by Alzheimer's disease. While high levels of DHCR24/seladin-1 exert antiapoptotic function by conferring resistance against oxidative stress, the molecular mechanism for this protective effect is not fully understood....

journal_title：Molecular and cellular biology

authors： Kuehnle K,Crameri A,Kälin RE,Luciani P,Benvenuti S,Peri A,Ratti F,Rodolfo M,Kulic L,Heppner FL,Nitsch RM,Mohajeri MH

更新日期：2008-01-01 00:00:00

abstract：:Hybridization-complementation studies indicated that mutations in multiple genes can render Chinese hamster cells resistant to the alkaloid translation inhibitor emetine. Two of the genes, emtA and emtB, recognized in Chinese hamster lung and ovary cell lines, respectively, are known to affect the ribosomes of the cel...

journal_title：Molecular and cellular biology

authors： Nielsen-Smith K,McGill S,Frahm M,Roufa DJ

更新日期：1983-02-01 00:00:00

abstract：:We have previously shown that activation of extracellular signal-regulated kinase (Erk) by epidermal growth factor (EGF) treatment was significantly decreased in mouse fibroblast cells expressing a mutant Shp-2 molecule lacking 65 amino acids in the SH2-N domain, Shp-2(Delta46-110). To address the molecular mechanism ...

journal_title：Molecular and cellular biology

authors： Shi ZQ,Yu DH,Park M,Marshall M,Feng GS

更新日期：2000-03-01 00:00:00

abstract：:The homeo box gene even-skipped (eve) encodes a 376-amino-acid protein that binds with high affinity to sequences located near the 5' termini of the eve and en genes. The 5' en sites are A + T rich and contain copies of the 10-base-pair (bp) consensus sequence T-C-A-A-T-T-A-A-A-T. In contrast, the 5' eve sites are G +...

journal_title：Molecular and cellular biology

authors： Hoey T,Warrior R,Manak J,Levine M

更新日期：1988-11-01 00:00:00

abstract：:Gene expression programs required for differentiation depend on both DNA-bound transcription factors and surrounding histone modifications. Expression of the basic helix-loop-helix (bHLH) protein NeuroD1 is restricted to endocrine cells in the gastrointestinal (GI) tract, where it is important for endocrine differenti...

journal_title：Molecular and cellular biology

authors： Ray SK,Li HJ,Metzger E,Schüle R,Leiter AB

更新日期：2014-06-01 00:00:00

abstract：:The Drosophila melanogaster Myb-MuvB/dREAM complex (MMB/dREAM) participates in both the activation and repression of developmentally regulated genes and origins of DNA replication. Mutants in MMB subunits exhibit diverse phenotypes, including lethality, eye defects, reduced fecundity, and sterility. Here, we used P-el...

journal_title：Molecular and cellular biology

authors： Lewis PW,Sahoo D,Geng C,Bell M,Lipsick JS,Botchan MR

更新日期：2012-08-01 00:00:00

abstract：:The gene encoding human hemojuvelin (HJV) is one of the genes that, when mutated, can cause juvenile hemochromatosis, an early-onset inherited disorder associated with iron overload. The 5' untranslated region of the human HJV mRNA has two upstream open reading frames (uORFs), with 28 and 19 codons formed by two upstr...

journal_title：Molecular and cellular biology

authors： Onofre C,Tomé F,Barbosa C,Silva AL,Romão L

更新日期：2015-04-01 00:00:00

abstract：:Mutations in polycystin 2 (PC2), a Ca(2+)-permeable cation channel, cause autosomal dominant polycystic kidney disease. Whether PC2 functions in the endoplasmic reticulum (ER) or in the plasma membrane has been controversial. Here we generated and characterized a polyclonal antibody against PC2, determined the subcell...

journal_title：Molecular and cellular biology

authors： Luo Y,Vassilev PM,Li X,Kawanabe Y,Zhou J

更新日期：2003-04-01 00:00:00

abstract：:The accumulation of misfolded proteins stresses the endoplasmic reticulum (ER) and triggers cell death through activation of the multidomain proapoptotic BCL-2 proteins BAX and BAK at the outer mitochondrial membrane. The signaling events that connect ER stress with the mitochondrial apoptotic machinery remain unclear...

journal_title：Molecular and cellular biology

authors： Upton JP,Austgen K,Nishino M,Coakley KM,Hagen A,Han D,Papa FR,Oakes SA

更新日期：2008-06-01 00:00:00

abstract：:The genetic basis of pancreatic ductal adenocarcinoma, which constitutes the most common type of pancreatic malignancy, involves the sequential activation of oncogenes and inactivation of tumor suppressor genes. Among the pivotal genetic alterations are Ki-RAS oncogene activation and p53 tumor suppressor gene inactiva...

journal_title：Molecular and cellular biology

authors： Deramaudt TB,Takaoka M,Upadhyay R,Bowser MJ,Porter J,Lee A,Rhoades B,Johnstone CN,Weissleder R,Hingorani SR,Mahmood U,Rustgi AK

更新日期：2006-06-01 00:00:00

abstract：:Giardia lamblia trophozoites demonstrate variable expression of a repertoire of cysteine-rich surface antigens in vitro and in vivo. The size of the repertoire has been estimated at 20 to 184, and specific variants can be detected after approximately 12 generations of in vitro growth for the WB isolate. In earlier stu...

journal_title：Molecular and cellular biology

authors： Adam RD,Yang YM,Nash TE

更新日期：1992-03-01 00:00:00

abstract：:A transgenic gpt+ Chinese hamster cell line (G12) was found to be susceptible to carcinogenic nickel-induced inactivation of gpt expression without mutagenesis or deletion of the transgene. Many nickel-induced 6-thioguanine-resistant variants spontaneously reverted to actively express gpt, as indicated by both reversi...

journal_title：Molecular and cellular biology

authors： Lee YW,Klein CB,Kargacin B,Salnikow K,Kitahara J,Dowjat K,Zhitkovich A,Christie NT,Costa M

更新日期：1995-05-01 00:00:00

abstract：:Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two heat-sensitive and two cold-sensitive isolates were characterized. The growth of the four mutants is inhibited at their ...

journal_title：Molecular and cellular biology

authors： Moritz M,Pulaski BA,Woolford JL Jr

更新日期：1991-11-01 00:00:00

abstract：:We measured the content and metabolism of histone mRNA in mouse 3T6 fibroblasts during a serum-induced transition from the resting to growing state. The content of several histone H3 and H2b mRNAs was measured by an S1 nuclease procedure. All of these increase in parallel by a factor of about 50 during S phase. Howeve...

journal_title：Molecular and cellular biology

authors： DeLisle AJ,Graves RA,Marzluff WF,Johnson LF

更新日期：1983-11-01 00:00:00

abstract：:The steroid hormone progesterone acts via high-affinity nuclear receptors that interact with specific DNA sequences located near the promoter of the hormone-responsive gene. Recent studies suggested that the hormone-occupied progesterone receptor (PR) mediates gene activation by recruiting a cellular coregulatory fact...

journal_title：Molecular and cellular biology

authors： Xu Y,Klein-Hitpass L,Bagchi MK

更新日期：2000-03-01 00:00:00

abstract：:The E2F transcription factors are key downstream targets of the retinoblastoma protein (pRB) tumor suppressor. We have previously shown that E2F3 plays a critical role in mediating the mitogen-induced activation of E2F-responsive genes and contributes to both the inappropriate proliferation and the p53-dependent apopt...

journal_title：Molecular and cellular biology

authors： Ziebold U,Lee EY,Bronson RT,Lees JA

更新日期：2003-09-01 00:00:00

abstract：:In the sea urchin embryo, late histone genes are transcribed at low levels during cleavage and blastula formation and at substantially higher levels in later stages of embryogenesis. To investigate the molecular basis of the stage-specific expression of a late H2B histone gene, we injected mutant genes lacking portion...

journal_title：Molecular and cellular biology

authors： Zhao AZ,Colin AM,Bell J,Baker M,Char BR,Maxson R

更新日期：1990-12-01 00:00:00

abstract：:USF is a family of basic helix-loop transcriptional factors that recognizes DNA-binding sites similar to those of the Myc oncoproteins. Here, various functional domains in the mouse USF2 protein were identified and characterized. Indirect immunofluorescence studies with transiently transfected cells revealed that both...

journal_title：Molecular and cellular biology

authors： Luo X,Sawadogo M

更新日期：1996-04-01 00:00:00

abstract：:T cell antigen receptor (TCR) activation triggers profound changes in the actin cytoskeleton. In addition to controlling cellular shape and polarity, this process regulates vital T cell responses, such as T cell adhesion, motility, and proliferation. These depend on the recruitment of the signaling proteins Nck and Wi...

journal_title：Molecular and cellular biology

authors： Pauker MH,Reicher B,Fried S,Perl O,Barda-Saad M

更新日期：2011-07-01 00:00:00

abstract：:The interaction of Prep1 and Pbx homeodomain transcription factors regulates their activity, nuclear localization, and likely, function in development. To understand the in vivo role of Prep1, we have analyzed an embryonic lethal hypomorphic mutant mouse (Prep1(i/i)). Prep1(i/i) embryos die at embryonic day 17.5 (E17....

journal_title：Molecular and cellular biology

authors： Ferretti E,Villaescusa JC,Di Rosa P,Fernandez-Diaz LC,Longobardi E,Mazzieri R,Miccio A,Micali N,Selleri L,Ferrari G,Blasi F

更新日期：2006-08-01 00:00:00

abstract：:We have studied the abilities of different transactivation domains to stimulate the initiation and elongation (postinitiation) steps of RNA polymerase II transcription in vivo. Nuclear run-on and RNase protection analyses revealed three classes of activation domains: Sp1 and CTF stimulated initiation (type I); human i...

journal_title：Molecular and cellular biology

authors： Blau J,Xiao H,McCracken S,O'Hare P,Greenblatt J,Bentley D

更新日期：1996-05-01 00:00:00

abstract：:The Reelin signaling cascade plays a crucial role in the correct positioning of neurons during embryonic brain development. Reelin binding to apolipoprotein E receptor 2 (ApoER2) and very-low-density-lipoprotein receptor (VLDLR) leads to phosphorylation of disabled 1 (Dab1), an adaptor protein which associates with th...

journal_title：Molecular and cellular biology

authors： Strasser V,Fasching D,Hauser C,Mayer H,Bock HH,Hiesberger T,Herz J,Weeber EJ,Sweatt JD,Pramatarova A,Howell B,Schneider WJ,Nimpf J

更新日期：2004-02-01 00:00:00

abstract：:We report that newly synthesized mRNA poly(A) tails are matured to precise lengths by the Pab1p-dependent poly(A) nuclease (PAN) of Saccharomyces cerevisiae. These results provide evidence for an initial phase of mRNA deadenylation that is required for poly(A) tail length control. In RNA 3'-end processing extracts lac...

journal_title：Molecular and cellular biology

authors： Brown CE,Sachs AB

更新日期：1998-11-01 00:00:00

abstract：:Insulin gene transcription relies on enhancer and promoter elements which are active in pancreatic beta cells. We showed that adenovirus type 5 infection of HIT T-15 cells, a transformed hamster beta cell line, represses insulin gene transcription and mRNA levels. Using expression plasmids transiently introduced into ...

journal_title：Molecular and cellular biology

authors： Stein RW,Ziff EB

更新日期：1987-03-01 00:00:00

abstract：:Two members of the heat shock transcription factor (HSF) family, HSF1 and HSF2, both function as transcriptional activators of heat shock gene expression. However, the inducible DNA-binding activities of these two factors are regulated by distinct pathways. HSF1 is activated by heat shock and other forms of stress, wh...

journal_title：Molecular and cellular biology

authors： Sistonen L,Sarge KD,Morimoto RI

更新日期：1994-03-01 00:00:00

abstract：:The recruitment of transcriptional coactivators, including histone modifying enzymes, is an important step in transcription regulation. A typical activator is thought to interact with several cofactors, presumably in a sequential manner. The common use of several cofactors raises the question of how activators achieve...

journal_title：Molecular and cellular biology

authors： Gamper AM,Roeder RG

更新日期：2008-04-01 00:00:00

abstract：:Repeat-induced point mutation (RIP) is a process that efficiently detects DNA duplications prior to meiosis in Neurospora crassa and peppers them with G:C to A:T mutations. Cytosine methylation is typically associated with sequences affected by RIP, and methylated cytosines are not limited to CpG dinucleotides. We gen...

journal_title：Molecular and cellular biology

authors： Singer MJ,Marcotte BA,Selker EU

更新日期：1995-10-01 00:00:00