Transforming ras proteins accelerate hormone-induced maturation and stimulate cyclic AMP phosphodiesterase in Xenopus oocytes.

abstract：:Temperature-sensitive mutants of TFIIB that are defective for essential interactions were isolated. One mutation (G204D) results in disruption of a protein-protein contact between TFIIB and TATA binding protein (TBP), while the other (K272I) disrupts an interaction between TFIIB and DNA. The TBP gene was mutagenized, ...

journal_title：Molecular and cellular biology

authors： Buratowski RM,Downs J,Buratowski S

更新日期：2002-12-01 00:00:00

abstract：:The Csk tyrosine kinase negatively regulates the Src family kinases Lck and Fyn in T cells. Engagement of the T-cell antigen receptor results in a removal of Csk from the lipid raft-associated transmembrane protein PAG/Cbp. Instead, Csk becomes associated with an approximately 72-kDa tyrosine-phosphorylated protein, w...

journal_title：Molecular and cellular biology

authors： Rahmouni S,Vang T,Alonso A,Williams S,van Stipdonk M,Soncini C,Moutschen M,Schoenberger SP,Mustelin T

更新日期：2005-03-01 00:00:00

abstract：:Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding ...

journal_title：Molecular and cellular biology

authors： Zerby D,Chen CJ,Poon E,Lee D,Shiekhattar R,Lieberman PM

更新日期：1999-03-01 00:00:00

abstract：:The first processing event that mouse pre-rRNA undergoes occurs within the external transcribed spacer and is efficiently reproduced in vitro. Analysis with nondenaturing polyacrylamide gels revealed the formation of heparin-resistant complexes of retarded electrophoretic mobility on the substrate rRNA. The specificit...

journal_title：Molecular and cellular biology

authors： Kass S,Sollner-Webb B

更新日期：1990-09-01 00:00:00

abstract：:The biosynthesis of influenza virus hemagglutinin (HA) and its translocation across microsomal membranes were studied in a mammalian cell-free system. All forms of HA could be cotranslationally translocated with high efficiency. However, only truncated forms of HA were translocated after protein synthesis has been ter...

journal_title：Molecular and cellular biology

authors： Chao CC,Bird P,Gething MJ,Sambrook J

更新日期：1987-10-01 00:00:00

abstract：:The immunoglobulin superfamily recognition molecule L1 plays important functional roles in the developing and adult nervous system. Metalloprotease-mediated cleavage of this adhesion molecule has been shown to stimulate cellular migration and neurite outgrowth. We demonstrate here that L1 cleavage is mediated by two d...

journal_title：Molecular and cellular biology

authors： Maretzky T,Schulte M,Ludwig A,Rose-John S,Blobel C,Hartmann D,Altevogt P,Saftig P,Reiss K

更新日期：2005-10-01 00:00:00

abstract：:The dynamic SCF (Skp1-cullin1-F-box protein) assembly is controlled by cycles of cullin neddylation/deneddylation based on the deneddylation activity of the COP9 signalosome (CSN) and global sequestration of cullins by CAND1. However, acceptance of this prediction was hampered by the results of recent studies, and the...

journal_title：Molecular and cellular biology

authors： Liu Q,Zhou Y,Tang R,Wang X,Hu Q,Wang Y,He Q

更新日期：2017-11-13 00:00:00

abstract：:The inducible transcriptional complex AP-1, composed of c-Fos and c-Jun proteins, is crucial for cell adaptation to many environmental changes. While its mechanisms of activation have been extensively studied, how its activity is restrained is poorly understood. We report here that lysine 265 of c-Fos is conjugated by...

journal_title：Molecular and cellular biology

authors： Bossis G,Malnou CE,Farras R,Andermarcher E,Hipskind R,Rodriguez M,Schmidt D,Muller S,Jariel-Encontre I,Piechaczyk M

更新日期：2005-08-01 00:00:00

abstract：:The fgr proto-oncogene encodes a nonreceptor protein-tyrosine kinase, designated p55c-fgr. In this study, we have isolated human fgr cDNA molecules from normal monocyte mRNA templates. Nucleotide sequence analysis of the longest fgr cDNA revealed a 5' untranslated region of 927 bp which included two Alu-like repeats a...

journal_title：Molecular and cellular biology

authors： Gutkind JS,Link DC,Katamine S,Lacal P,Miki T,Ley TJ,Robbins KC

更新日期：1991-03-01 00:00:00

abstract：:We have used extrachromosomal substrates carrying immunoglobulin heavy-chain S mu and S gamma 3 switch region sequences to study activation and targeting of recombination by a transcriptional enhancer element. Substrates are transiently introduced into activated primary murine B cells, in which recombination involving...

journal_title：Molecular and cellular biology

authors： Leung H,Maizels N

更新日期：1994-02-01 00:00:00

abstract：:A late region deletion mutant of simian virus 40 (dl5) was previously shown to be deficient in the transport of nuclear RNA. This is a splice junction deletion that has lost the 3' end of an RNA leader, an intervening sequence, and the 5' end of the splice acceptor site on the body of the mRNA. In this report, we anal...

journal_title：Molecular and cellular biology

authors： Villarreal LP,White RT

更新日期：1983-08-01 00:00:00

abstract：:Plasmids carrying the herpes simplex virus thymidine kinase (tk) gene were used to transfect thymidine kinase-deficient cells of the mouse fibroblast cell line LM(tk-). Individual cell clones were cultivated in selective hypoxanthine-aminopterin-thymidine medium to determine the number of integrated plasmid copies whi...

journal_title：Molecular and cellular biology

authors： Pülm W,Knippers R

更新日期：1985-02-01 00:00:00

abstract：:Precursor RNA containing the adenovirus L3 polyadenylation site is assembled into a 50S complex upon incubation with HeLa nuclear extract at 30 degrees C. The cofactor and sequence requirements for 50S complex formation are similar to those of the in vitro polyadenylation reaction. Assembly of this complex requires AT...

journal_title：Molecular and cellular biology

authors： Moore CL,Skolnik-David H,Sharp PA

更新日期：1988-01-01 00:00:00

abstract：:We have characterized two P-element-induced, lethal mutations in Drosophila melanogaster which affect the larval hemocytes, mediators of the insect immune response. Each mutant displays larval melanotic tumors characteristic of mutations affecting the insect cellular immune system, and the moribund animals develop gro...

journal_title：Molecular and cellular biology

authors： Stewart MJ,Denell R

更新日期：1993-04-01 00:00:00

abstract：:In selective autophagy, the adaptor protein SQSTM1/p62 plays a critical role in recognizing/loading cargo (e.g., malfolded proteins) into autophagosomes for lysosomal degradation. Here we report that whereas SQSTM1/p62 levels fluctuated in a time-dependent manner during autophagy, inhibition or knockdown of Cdk9/cycli...

journal_title：Molecular and cellular biology

authors： Chen S,Zhou L,Zhang Y,Leng Y,Pei XY,Lin H,Jones R,Orlowski RZ,Dai Y,Grant S

更新日期：2014-09-15 00:00:00

abstract：:DHCR24/seladin-1, a crucial enzyme in sterol synthesis, is of lower abundance in brain areas affected by Alzheimer's disease. While high levels of DHCR24/seladin-1 exert antiapoptotic function by conferring resistance against oxidative stress, the molecular mechanism for this protective effect is not fully understood....

journal_title：Molecular and cellular biology

authors： Kuehnle K,Crameri A,Kälin RE,Luciani P,Benvenuti S,Peri A,Ratti F,Rodolfo M,Kulic L,Heppner FL,Nitsch RM,Mohajeri MH

更新日期：2008-01-01 00:00:00

abstract：:To understand the function of SPT13, a gene encoding a trans-acting factor involved in regulation of Ty-mediated gene expression, we have cloned and sequenced the gene. Our analysis revealed that SPT13 is the same gene as GAL11, a gene in which mutations cause reduced expression of some GAL4-regulated genes. Further a...

journal_title：Molecular and cellular biology

authors： Fassler JS,Winston F

更新日期：1989-12-01 00:00:00

abstract：:We have studied the patterns of expression of four B-type cyclins (Clbs), Clb1, Clb2, Clb3, and Clb4, and their ability to activate p34cdc28 during the mitotic and meiotic cell cycles of Saccharomyces cerevisiae. During the mitotic cell cycle, Clb3 and Clb4 were expressed and induced a kinase activity in association w...

journal_title：Molecular and cellular biology

authors： Grandin N,Reed SI

更新日期：1993-04-01 00:00:00

abstract：:The Saccharomyces cerevisiae PGK (phosphoglycerate kinase) gene encodes one of the most abundant mRNA and protein species in the cell. To identify the promoter sequences required for the efficient expression of PGK, we undertook a detailed internal deletion analysis of the 5' noncoding region of the gene. Our analysis...

journal_title：Molecular and cellular biology

authors： Ogden JE,Stanway C,Kim S,Mellor J,Kingsman AJ,Kingsman SM

更新日期：1986-12-01 00:00:00

abstract：:We previously purified and characterized functionally the Escherichia coli-expressed product of the human subgroup C adenovirus E1A 13S mRNA (B. Ferguson, N. Jones, J. Richter, and M. Rosenberg, Science 224:1343-1346, 1984; B. Krippl, B. Ferguson, M. Rosenberg, and H. Westphal, Proc. Natl. Acad. Sci. USA 81:6988-6992,...

journal_title：Molecular and cellular biology

authors： Ferguson B,Krippl B,Andrisani O,Jones N,Westphal H,Rosenberg M

更新日期：1985-10-01 00:00:00

abstract：:Numb proteins are evolutionarily conserved signaling molecules that make the daughter cells different after asymmetric divisions by segregating to only one daughter. They contain distinct binding motifs for alpha-adaptin (alpha-Ada) and proteins with Eps15 homology (EH) domains, which regulate endocytosis, and for E3 ...

journal_title：Molecular and cellular biology

authors： Tang H,Rompani SB,Atkins JB,Zhou Y,Osterwalder T,Zhong W

更新日期：2005-04-01 00:00:00

abstract：:Very little is known about the mechanisms mediating longevities of mRNAs. As a means of identifying potential cis- and trans-acting elements which stabilize an individual mRNA, naturally occurring mutations that decreased stability of the normally long-lived globin mRNA were analyzed. Our previous studies demonstrated...

journal_title：Molecular and cellular biology

authors： Weiss IM,Liebhaber SA

更新日期：1995-05-01 00:00:00

abstract：:To overcome the difficulty of assessing oncogene action in human epithelial cell types, such as thyroid, which have limited proliferative potential in culture, we have explored the use of temperature-sensitive (ts) mutants of simian virus 40 (SV40) early region to create conditionally immortalized epithelial cell line...

journal_title：Molecular and cellular biology

authors： Wynford-Thomas D,Bond JA,Wyllie FS,Burns JS,Williams ED,Jones T,Sheer D,Lemoine NR

更新日期：1990-10-01 00:00:00

abstract：:Small nucleolar RNAs (snoRNAs) orchestrate the modification and cleavage of pre-rRNA and are essential for ribosome biogenesis. Recent data suggest that after nucleoplasmic synthesis, snoRNAs transiently localize to the Cajal body (in plant and animal cells) or the homologous nucleolar body (in budding yeast) for matu...

journal_title：Molecular and cellular biology

authors： Qiu H,Eifert J,Wacheul L,Thiry M,Berger AC,Jakovljevic J,Woolford JL Jr,Corbett AH,Lafontaine DL,Terns RM,Terns MP

更新日期：2008-06-01 00:00:00

abstract：:An 85,000-molecular-weight polypeptide (85K polypeptide) has previously been identified as a common substrate for tyrosine phosphorylation upon polyomavirus middle T transformation or upon platelet-derived growth factor stimulation of 3T3 cells. In each case, pp85 has an associated phosphatidylinositol kinase activity...

journal_title：Molecular and cellular biology

authors： Cohen B,Liu YX,Druker B,Roberts TM,Schaffhausen BS

更新日期：1990-06-01 00:00:00

abstract：:Repeat-induced point mutation (RIP) is a process that efficiently detects DNA duplications prior to meiosis in Neurospora crassa and peppers them with G:C to A:T mutations. Cytosine methylation is typically associated with sequences affected by RIP, and methylated cytosines are not limited to CpG dinucleotides. We gen...

journal_title：Molecular and cellular biology

authors： Singer MJ,Marcotte BA,Selker EU

更新日期：1995-10-01 00:00:00

abstract：:Homologous recombination is now routinely used in mammalian cells to replace endogenous chromosomal sequences with transferred DNA. Vectors for this purpose are traditionally constructed so that the replacement segment is flanked on both sides by DNA sequences which are identical to sequences in the chromosomal target...

journal_title：Molecular and cellular biology

authors： Berinstein N,Pennell N,Ottaway CA,Shulman MJ

更新日期：1992-01-01 00:00:00

abstract：:Multiple members of the A, B, and C clusters of Hox genes are expressed in hematopoietic cells. Several of these Hox genes have been found to display distinctive expression patterns, with genes located at the 3' side of the clusters being expressed at their highest levels in the most primitive subpopulation of human C...

journal_title：Molecular and cellular biology

authors： Thorsteinsdottir U,Sauvageau G,Hough MR,Dragowska W,Lansdorp PM,Lawrence HJ,Largman C,Humphries RK

更新日期：1997-01-01 00:00:00

abstract：:Podocytes are highly differentiated and polarized epithelial cells located on the visceral side of the glomerulus. They form an indispensable component of the glomerular filter, the slit diaphragm, formed by several transmembrane proteins and adaptor molecules. Disruption of the slit diaphragm can lead to massive prot...

journal_title：Molecular and cellular biology

authors： Tossidou I,Niedenthal R,Klaus M,Teng B,Worthmann K,King BL,Peterson KJ,Haller H,Schiffer M

更新日期：2012-03-01 00:00:00

abstract：:Three ras homologs have been identified in Drosophila melanogaster. Here we describe the tissue distribution of their transcripts as analyzed by in situ hybridization. The RNAs of the three genes show a similar distribution at every developmental stage examined. In embryos, the transcripts are uniformly distributed. I...

journal_title：Molecular and cellular biology

authors： Segal D,Shilo BZ

更新日期：1986-06-01 00:00:00