Identification of genes that function in the biogenesis and localization of small nucleolar RNAs in Saccharomyces cerevisiae.

abstract：:The GTPase-activating protein (GAP) stimulates the GTPase reaction of p21 by 5 orders of magnitude such that the kcat of the reaction is increased to 19 s-1. Mutations of residues in loop L1 (Gly-12 and Gly-13), in loop L2 (Thr-35 and Asp-38), and in loop L4 (Gln-61 and Glu-63) influence the reaction in different ways...

journal_title：Molecular and cellular biology

authors： Gideon P,John J,Frech M,Lautwein A,Clark R,Scheffler JE,Wittinghofer A

更新日期：1992-05-01 00:00:00

abstract：:Aberrant activation of the Wnt/β-catenin pathway and polo-like kinase 1 (Plk1) overexpression represent two common events in prostate cancer with relevant functional implications. This minireview analyzes their potential therapeutic significance in prostate cancer based on their role as androgen receptor (AR) signalin...

journal_title：Molecular and cellular biology

authors： Cristóbal I,Rojo F,Madoz-Gúrpide J,García-Foncillas J

更新日期：2016-05-31 00:00:00

abstract：:The mitochondrial genome of trypanosomes is composed of thousands of topologically interlocked circular DNA molecules that form the kinetoplast DNA (kDNA). Most genes encoded by the kDNA require a posttranscriptional modification process called RNA editing to form functional mRNAs. Here, we show that alternative editi...

journal_title：Molecular and cellular biology

authors： Ochsenreiter T,Anderson S,Wood ZA,Hajduk SL

更新日期：2008-09-01 00:00:00

abstract：:The RPC31 gene encoding the C31 subunit of Saccharomyces cerevisiae RNA polymerase C (III) has been isolated, starting from a C-terminal fragment cloned on a lambda gt11 library. It is unique on the yeast genome and lies on the left arm of chromosome XIV, very close to a NotI site. Its coding sequence perfectly matche...

journal_title：Molecular and cellular biology

authors： Mosrin C,Riva M,Beltrame M,Cassar E,Sentenac A,Thuriaux P

更新日期：1990-09-01 00:00:00

abstract：:Heteroduplexes were prepared from two plasmids, pRH4-14/TK and pRH5-8/TK, containing different amber mutations in the neomycin resistance gene (Neor). The Neor gene was engineered to be expressed in both bacterial and mammalian cells. A functional Neor gene conferred kanamycin resistance to bacteria and resistance to ...

journal_title：Molecular and cellular biology

authors： Folger KR,Thomas K,Capecchi MR

更新日期：1985-01-01 00:00:00

abstract：:In yeast, the pheromone alpha-factor acts as an antiproliferative factor that induces G1 arrest and cellular differentiation. Previous data have indicated that Far1, a factor dedicated to pheromone-induced cell cycle arrest, is under positive and negative posttranslational regulation. Phosphorylation by the pheromone-...

journal_title：Molecular and cellular biology

authors： Gartner A,Jovanović A,Jeoung DI,Bourlat S,Cross FR,Ammerer G

更新日期：1998-07-01 00:00:00

abstract：:2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other aryl hydrocarbon receptor (AhR) ligands suppress 17beta-estradiol (E)-induced responses in the rodent uterus and mammary tumors and in human breast cancer cells. Treatment of ZR-75, T47D, and MCF-7 human breast cancer cells with TCDD induces proteasome-dependent deg...

journal_title：Molecular and cellular biology

authors： Wormke M,Stoner M,Saville B,Walker K,Abdelrahim M,Burghardt R,Safe S

更新日期：2003-03-01 00:00:00

abstract：:In Saccharomyces cerevisiae, transcriptional activation mediated by the GAL4 regulatory protein is repressed in the absence of galactose by the binding of the GAL80 protein, an interaction that requires the carboxy-terminal 28 amino acids of GAL4. The homolog of GAL4 from Kluyveromyces lactis, LAC9, activates transcri...

journal_title：Molecular and cellular biology

authors： Salmeron JM Jr,Langdon SD,Johnston SA

更新日期：1989-07-01 00:00:00

abstract：:RBM4 promotes differentiation of neuronal progenitor cells and neurite outgrowth of cultured neurons via its role in splicing regulation. In this study, we further explored the role of RBM4 in neuronal differentiation. During neuronal differentiation, energy production shifts from glycolysis to oxidative phosphorylati...

journal_title：Molecular and cellular biology

authors： Su CH,Hung KY,Hung SC,Tarn WY

更新日期：2017-01-19 00:00:00

abstract：:Treatment of cells with tumor-promoting phorbol esters results in the activation but then depletion of phorbol ester-responsive protein kinase C (PKC) isoforms. The ubiquitin-proteasome pathway has been implicated in regulating the levels of many cellular proteins, including those involved in cell cycle control. We re...

journal_title：Molecular and cellular biology

authors： Lu Z,Liu D,Hornia A,Devonish W,Pagano M,Foster DA

更新日期：1998-02-01 00:00:00

abstract：:Budding yeast polo kinase Cdc5p localizes to the spindle pole body (SPB) and to the bud-neck and plays multiple roles during M-phase progression. To dissect localization-specific mitotic functions of Cdc5p, we tethered a localization-defective N-terminal kinase domain of Cdc5p (Cdc5pDeltaC) to the SPB or to the bud-ne...

journal_title：Molecular and cellular biology

authors： Park JE,Park CJ,Sakchaisri K,Karpova T,Asano S,McNally J,Sunwoo Y,Leem SH,Lee KS

更新日期：2004-11-01 00:00:00

abstract：:Cotranslational insertion of type I collagen chains into the lumen of the endoplasmic reticulum (ER) and their subsequent folding into a heterotrimeric helix is a complex process which requires coordinated action of the translation machinery, components of translocons, molecular chaperones, and modifying enzymes. Here...

journal_title：Molecular and cellular biology

authors： Stefanovic B,Stefanovic L,Schnabl B,Bataller R,Brenner DA

更新日期：2004-02-01 00:00:00

abstract：:Mouse mammary epithelial cells can be transformed in primary cultures to preneoplastic and neoplastic states when treated with N-methyl-N-nitrosourea (MNU). Mammary carcinomas arising from MNU-induced hyperplastic alveolar nodules (a type of mouse mammary preneoplastic lesion) contained transforming c-Ki-ras genes whe...

journal_title：Molecular and cellular biology

authors： Miyamoto S,Sukumar S,Guzman RC,Osborn RC,Nandi S

更新日期：1990-04-01 00:00:00

abstract：:The Moloney murine sarcoma virus long terminal repeat (LTR) harbors two distinct positive activators of transcription, namely, a distal signal and an enhancer. In this report we demonstrate that infection by herpes simplex virus (HSV) can markedly affect the utilization of these two Moloney murine sarcoma virus transc...

journal_title：Molecular and cellular biology

authors： Graves BJ,Eisenberg SP,Coen DM,McKnight SL

更新日期：1985-08-01 00:00:00

abstract：:Entry into mitosis requires the activity of the Cdc2 kinase. Cdc2 associates with the B-type cyclins, and the Cdc2-cyclin B heterodimer is in turn regulated by phosphorylation. Phosphorylation of threonine 161 is required for the Cdc2-cyclin B complex to be catalytically active, whereas phosphorylation of threonine 14...

journal_title：Molecular and cellular biology

authors： Liu F,Stanton JJ,Wu Z,Piwnica-Worms H

更新日期：1997-02-01 00:00:00

abstract：:Gamma interferon (IFN-γ) is an inflammatory cytokine that has complex effects on myogenesis. Here, we show that the IFN-γ-induced inhibition of myogenesis is mediated by the major histocompatibility complex (MHC) class II transactivator, CIITA, which binds to myogenin and inhibits its activity. In IFN-γ-treated myobla...

journal_title：Molecular and cellular biology

authors： Londhe P,Davie JK

更新日期：2011-07-01 00:00:00

abstract：:Telomere maintenance is required for chromosome stability, and telomeres are typically replicated by the action of the reverse transcriptase telomerase. In both tumor and yeast cells that lack telomerase, telomeres are maintained by an alternative recombination mechanism. Genetic studies have led to the identification...

journal_title：Molecular and cellular biology

authors： Tsai YL,Tseng SF,Chang SH,Lin CC,Teng SC

更新日期：2002-08-01 00:00:00

abstract：:To define the sequence elements involved in initiation of DNA synthesis at the simian virus 40 origin of replication, we determined the relative replication efficiencies in vitro and in vivo of templates containing a variety of mutations within the origin region. Replication of the mutants in vitro was assayed by the ...

journal_title：Molecular and cellular biology

authors： Li JJ,Peden KW,Dixon RA,Kelly T

更新日期：1986-04-01 00:00:00

abstract：:Notch signaling commences with two ligand-mediated proteolysis events that release the Notch intracellular domain, NICD, from the plasma membrane. NICD then translocates into the nucleus and interacts with the DNA binding protein CSL to activate transcription. We found that NICD expression also potentiates activity of...

journal_title：Molecular and cellular biology

authors： Ross DA,Kadesch T

更新日期：2001-11-01 00:00:00

abstract：:A gene encoding a novel CACCC box-binding protein that binds to the promoter region of the human T-cell receptor (TCR) V beta 8.1 gene and the mouse TCR alpha gene silencer has been cloned. This gene, termed ht beta, contains four zinc fingers of the class Cys2-X12-His2 that may be responsible for DNA binding and a hi...

journal_title：Molecular and cellular biology

authors： Wang Y,Kobori JA,Hood L

更新日期：1993-09-01 00:00:00

abstract：:The tyrosinase family comprises three members, tyrosinase (Tyr), tyrosinase-related protein 1 (Tyrp1), and dopachrome tautomerase (Dct). Null mutations and deletions at the Tyr and Tyrp1 loci are known and phenotypically affect coat color due to the absence of enzyme or intracellular mislocalization. At the Dct locus,...

journal_title：Molecular and cellular biology

authors： Guyonneau L,Murisier F,Rossier A,Moulin A,Beermann F

更新日期：2004-04-01 00:00:00

abstract：:We have previously characterized a cloned cDNA coding for a developmentally regulated mRNA in Drosophila melanogaster whose expression is selectively regulated at the translational level during oogenesis and embryogenesis. In this report we show that this translationally regulated mRNA (rpA1) codes for an acidic ribos...

journal_title：Molecular and cellular biology

authors： Kay MA,Jacobs-Lorena M

更新日期：1985-12-01 00:00:00

abstract：:Smad6 and Smad7 are inhibitory Smads induced by transforming growth factor beta-Smad signal transduction pathways in a negative-feedback mechanism. Previously it has been thought that inhibitory Smads bind to the type I receptor and block the phosphorylation of receptor-activated Smads, thereby inhibiting the initiati...

journal_title：Molecular and cellular biology

authors： Lin X,Liang YY,Sun B,Liang M,Shi Y,Brunicardi FC,Shi Y,Feng XH

更新日期：2003-12-01 00:00:00

abstract：:Nitric oxide (NO) has been thought to regulate the immune system through S nitrosylation of the transcriptional factor NF-kappaB. However, regulatory effects of NO on innate immune responses are unclear. Here, we report that NO has a capability to control Toll-like receptor-mediated signaling through S nitrosylation. ...

journal_title：Molecular and cellular biology

authors： Into T,Inomata M,Nakashima M,Shibata K,Häcker H,Matsushita K

更新日期：2008-02-01 00:00:00

abstract：:Neutrophils are first responders rapidly mobilized to inflammatory sites by a tightly regulated, nonredundant hierarchy of chemoattractants. These chemoattractants engage neutrophil cell surface receptors triggering heterotrimeric G-protein Gα(i) subunits to exchange GDP for GTP. By limiting the duration that Gα(i) su...

journal_title：Molecular and cellular biology

authors： Cho H,Kamenyeva O,Yung S,Gao JL,Hwang IY,Park C,Murphy PM,Neubig RR,Kehrl JH

更新日期：2012-11-01 00:00:00

abstract：:Cooperation between STAT3 and c-Jun in driving transcription during transfection of reporter constructs is well established, and both proteins are present on some interleukin-6 (IL-6) STAT3-dependent promoters on chromosomal loci. We report that small interfering RNA knockdown of c-Jun or c-Fos diminishes IL-6 inducti...

journal_title：Molecular and cellular biology

authors： Ginsberg M,Czeko E,Müller P,Ren Z,Chen X,Darnell JE Jr

更新日期：2007-09-01 00:00:00

abstract：:Histone acetylation plays an important role in regulating chromatin structure and thus gene expression. Here we describe the functional characterization of HDAC4, a human histone deacetylase whose C-terminal part displays significant sequence similarity to the deacetylase domain of yeast HDA1. HDAC4 is expressed in va...

journal_title：Molecular and cellular biology

authors： Wang AH,Bertos NR,Vezmar M,Pelletier N,Crosato M,Heng HH,Th'ng J,Han J,Yang XJ

更新日期：1999-11-01 00:00:00

abstract：:Mitogen-activated protein kinases (MAPKs) play key roles in differentiation, growth, proliferation, and apoptosis. Although MAPKs have been extensively studied, the precise function, specific substrates, and target genes of each MAPK are not known. These issues could be addressed by sole activation of a given MAPK, e....

journal_title：Molecular and cellular biology

authors： Yaakov G,Bell M,Hohmann S,Engelberg D

更新日期：2003-07-01 00:00:00

abstract：:Paramecium tetraurelia contains two types of nuclei, a diploid germinal micronucleus and a large transcriptionally active macronucleus. The macronuclear genome is formed from the micronuclear DNA during sexual reproduction. Previous studies have shown that the processing of the A-type variable surface protein gene dur...

journal_title：Molecular and cellular biology

authors： Scott JM,Mikami K,Leeck CL,Forney JD

更新日期：1994-04-01 00:00:00

abstract：:The transcription factor NF-kappaB is overexpressed or constitutively activated in many cancer cells, where it induces expression of antiapoptotic genes correlating with resistance to anticancer therapies. Small molecules that inhibit the NF-kappaB signaling pathway could therefore be used to induce apoptosis in NF-ka...

journal_title：Molecular and cellular biology

authors： Bayon Y,Ortiz MA,Lopez-Hernandez FJ,Gao F,Karin M,Pfahl M,Piedrafita FJ

更新日期：2003-02-01 00:00:00