The human Myt1 kinase preferentially phosphorylates Cdc2 on threonine 14 and localizes to the endoplasmic reticulum and Golgi complex.

abstract：:Recognition of gene promoters by RNA polymerase II is mediated by general transcription factor IID (TFIID), which has been thought to be a static complex and to play a passive role in the regulation of gene expression under the instruction of gene-specific transcription factors. Here we show that transforming growth f...

journal_title：Molecular and cellular biology

authors： Nakagawa T,Hosogane M,Nakagawa M,Morohoshi A,Funayama R,Nakayama K

更新日期：2018-02-12 00:00:00

abstract：:Interferon A (IFN-A) genes are differentially expressed after virus induction. The differential expression of individual IFN-A genes is modulated by the specific transcription activators IFN regulatory factor 3 (IRF3) and IRF-7 and the homeoprotein transcription repressor Pitx1. We now show that repression by Pitx1 do...

journal_title：Molecular and cellular biology

authors： Island ML,Mesplede T,Darracq N,Bandu MT,Christeff N,Djian P,Drouin J,Navarro S

更新日期：2002-10-01 00:00:00

abstract：:Inactivation of the tumor suppressor p53 by degradation is a mechanism utilized by cells to adapt to endoplasmic reticulum (ER) stress. However, the mechanisms of p53 destabilization by ER stress are not known. We demonstrate here that the E3 ubiquitin-ligase Hdm2 is essential for the nucleocytoplasmic transport and p...

journal_title：Molecular and cellular biology

authors： Pluquet O,Qu LK,Baltzis D,Koromilas AE

更新日期：2005-11-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the SWI-SNF complex has been proposed to antagonize the repressive effects of chromatin by disrupting nucleosomes. The SIN genes were identified as suppressors of defects in the SWI-SNF complex, and the SIN1 gene encodes an HMG1-like protein that has been proposed to be a compone...

journal_title：Molecular and cellular biology

authors： Pérez-Martín J,Johnson AD

更新日期：1998-07-01 00:00:00

abstract：:A fragment of the human gene for c-erb-B was used to map homologous sequences in mice. Analysis of somatic cell hybrids and recombinant inbred and congenic mouse strains indicated that this gene, designated Erbb, is closely linked to the gene for alpha-globin on mouse chromosome 11. Several genes controlling hematopoi...

journal_title：Molecular and cellular biology

authors： Silver J,Whitney JB 3rd,Kozak C,Hollis G,Kirsch I

更新日期：1985-07-01 00:00:00

abstract：:Costimulation of G protein-coupled receptors (GPCRs) may result in cross talk interactions between their downstream signaling pathways. Stimulation of GPCRs may also lead to cross talk regulation of receptor tyrosine kinase signaling and thereby to activation of mitogen-activated protein kinase (MAPK). In COS-7 cells,...

journal_title：Molecular and cellular biology

authors： Hanke S,Nürnberg B,Groll DH,Liebmann C

更新日期：2001-12-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a potent angiogenic inducer that stimulates the expression of tissue factor (TF), the major cellular initiator of blood coagulation. Here we show that signaling triggered by VEGF induced DNA-binding and transcriptional activities of nuclear factor of activated T cells (NFAT...

journal_title：Molecular and cellular biology

authors： Armesilla AL,Lorenzo E,Gómez del Arco P,Martínez-Martínez S,Alfranca A,Redondo JM

更新日期：1999-03-01 00:00:00

abstract：:The tissue-specific expression of immunoglobulin genes can be partially explained by a requirement for activating factors found only in B lymphocytes and their derivatives. However, loss of immunoglobulin expression upon fusion of an immunoglobulin-producing myeloma cell with a T lymphoma cell (BW5147) or fibroblast (...

journal_title：Molecular and cellular biology

authors： Zaller DM,Yu H,Eckhardt LA

更新日期：1988-05-01 00:00:00

abstract：:Control of RNA turnover is a major, but poorly understood, aspect of gene regulation. In multicellular organisms, progress toward dissecting RNA turnover pathways has been made by defining some cis-acting sequences that function as either regulatory or cleavage targets (J. G. Belasco and G. Brawerman, Control of Messe...

journal_title：Molecular and cellular biology

authors： Bai C,Tolias PP

更新日期：1996-12-01 00:00:00

abstract：:Fragments of 5'-flanking and noncoding exon I sequences of the human gastrin gene were analyzed in transient expression assays after transfection of a variety of cell lines with the pSVCAT vector system. In the presence of the simian virus 40 (SV40) enhancer, the gastrin gene fragment from nucleotides -250 to +57, rel...

journal_title：Molecular and cellular biology

authors： Theill LE,Wiborg O,Vuust J

更新日期：1987-12-01 00:00:00

abstract：:The fibroblast growth factor (FGF) family consists of seven members whose activities are thought to be mediated by multiple receptors. Here we describe the cDNA cloning, expression, and characterization of a cysteine-rich FGF receptor (CFR) that is distinct from previously identified FGF receptors. The deduced amino a...

journal_title：Molecular and cellular biology

authors： Burrus LW,Zuber ME,Lueddecke BA,Olwin BB

更新日期：1992-12-01 00:00:00

abstract：:Little is known about the regions of RNA polymerase II (RNAPII) that are involved in the process of transcript elongation and interaction with elongation factors. One elongation factor, TFIIS, stimulates transcript elongation by binding to RNAPII and facilitating its passage through intrinsic pausing sites in vitro. I...

journal_title：Molecular and cellular biology

authors： Archambault J,Lacroute F,Ruet A,Friesen JD

更新日期：1992-09-01 00:00:00

abstract：:The biosynthesis, phosphorylation, and degradation of the epidermal growth factor (EGF) receptor were examined in normal human fibroblasts. The receptor was initially synthesized as an Mr = 160,000 immature form which matured to an Mr = 170,000 form in a monensin-sensitive manner. Tunicamycin treatment led to the accu...

journal_title：Molecular and cellular biology

authors： Decker SJ

更新日期：1984-09-01 00:00:00

abstract：:The synthesis of 60S ribosomal subunits in Saccharomyces cerevisiae requires Tif6p, the yeast homologue of mammalian eukaryotic translation initiation factor 6 (eIF6). In the present work, we have isolated a protein kinase from rabbit reticulocyte lysates on the basis of its ability to phosphorylate recombinant human ...

journal_title：Molecular and cellular biology

authors： Basu U,Si K,Deng H,Maitra U

更新日期：2003-09-01 00:00:00

abstract：:Compromised immunoregulation contributes to obesity and complications in metabolic pathogenesis. Here, we demonstrate that the nuclear factor of activated T cell (NFAT) group of transcription factors contributes to glucose and insulin homeostasis. Expression of two members of the NFAT family (NFATc2 and NFATc4) is ind...

journal_title：Molecular and cellular biology

authors： Yang TT,Suk HY,Yang X,Olabisi O,Yu RY,Durand J,Jelicks LA,Kim JY,Scherer PE,Wang Y,Feng Y,Rossetti L,Graef IA,Crabtree GR,Chow CW

更新日期：2006-10-01 00:00:00

abstract：:Growth factor independence 1B (GFI1B) coordinates assembly of transcriptional repressor complexes comprised of corepressors and histone-modifying enzymes to control gene expression programs governing lineage allocation in hematopoiesis. Enforced expression of GFI1B in K562 erythroleukemia cells favors erythroid over m...

journal_title：Molecular and cellular biology

authors： McClellan D,Casey MJ,Bareyan D,Lucente H,Ours C,Velinder M,Singer J,Lone MD,Sun W,Coria Y,Mason CC,Engel ME

更新日期：2019-06-13 00:00:00

abstract：:Homologous recombination is now routinely used in mammalian cells to replace endogenous chromosomal sequences with transferred DNA. Vectors for this purpose are traditionally constructed so that the replacement segment is flanked on both sides by DNA sequences which are identical to sequences in the chromosomal target...

journal_title：Molecular and cellular biology

authors： Berinstein N,Pennell N,Ottaway CA,Shulman MJ

更新日期：1992-01-01 00:00:00

abstract：:A late region deletion mutant of simian virus 40 (dl5) was previously shown to be deficient in the transport of nuclear RNA. This is a splice junction deletion that has lost the 3' end of an RNA leader, an intervening sequence, and the 5' end of the splice acceptor site on the body of the mRNA. In this report, we anal...

journal_title：Molecular and cellular biology

authors： Villarreal LP,White RT

更新日期：1983-08-01 00:00:00

abstract：:TRPV5 and TRPV6 are the most Ca2+-selective members of the transient receptor potential (TRP) family of cation channels and play a pivotal role in the maintenance of Ca2+ balance in the body. However, little is known about the mechanisms controlling the plasma membrane abundance of these channels to regulate epithelia...

journal_title：Molecular and cellular biology

authors： van de Graaf SF,Chang Q,Mensenkamp AR,Hoenderop JG,Bindels RJ

更新日期：2006-01-01 00:00:00

abstract：:Expression of the Saccharomyces cerevisiae CYC1 gene produces mRNA with more than 20 different 5' ends. A derivative of the CYC1 gene (CYC1-157) was constructed with a deletion of a portion of the CYC1 5'-noncoding region, which includes the sites at which many of the CYC1 mRNAs 5' ends map. A 54-mer double-stranded o...

journal_title：Molecular and cellular biology

authors： McNeil JB,Smith M

更新日期：1985-12-01 00:00:00

abstract：:Posttranslational modification of general transcription factors may be an important mechanism for global gene regulation. The general transcription factor IIA (TFIIA) binds to the TATA binding protein (TBP) and is essential for high-level transcription mediated by various activators. Modulation of the TFIIA-TBP intera...

journal_title：Molecular and cellular biology

authors： Solow SP,Lezina L,Lieberman PM

更新日期：1999-04-01 00:00:00

abstract：:A strong block to the elongation of nascent RNA transcripts by RNA polymerase II occurs in the 5' part of the mammalian c-fos proto-oncogene. In addition to the control of initiation, this mechanism contributes to transcriptional regulation of the gene. In vitro transcription experiments using nuclear extracts and pur...

journal_title：Molecular and cellular biology

authors： Mechti N,Piechaczyk M,Blanchard JM,Jeanteur P,Lebleu B

更新日期：1991-05-01 00:00:00

abstract：:Giardia lamblia trophozoites demonstrate variable expression of a repertoire of cysteine-rich surface antigens in vitro and in vivo. The size of the repertoire has been estimated at 20 to 184, and specific variants can be detected after approximately 12 generations of in vitro growth for the WB isolate. In earlier stu...

journal_title：Molecular and cellular biology

authors： Adam RD,Yang YM,Nash TE

更新日期：1992-03-01 00:00:00

abstract：:TRAF2 is an adaptor protein that regulates the activation of the c-Jun N-terminal kinase (JNK) and IkappaB kinase (IKK) signaling cascades in response to tumor necrosis factor alpha (TNF-alpha) stimulation. Although the downstream events in TNF-alpha signaling are better understood, the membrane-proximal events are st...

journal_title：Molecular and cellular biology

authors： Blackwell K,Zhang L,Thomas GS,Sun S,Nakano H,Habelhah H

更新日期：2009-01-01 00:00:00

abstract：:We reported the identification of a new family of DNA-binding proteins from our characterization of the dead ringer (dri) gene of Drosophila melanogaster. We show that dri encodes a nuclear protein that contains a sequence-specific DNA-binding domain that bears no similarity to known DNA-binding domains. A number of p...

journal_title：Molecular and cellular biology

authors： Gregory SL,Kortschak RD,Kalionis B,Saint R

更新日期：1996-03-01 00:00:00

abstract：:Mating-type genes resident in the silent cassette HML at the left arm of chromosome III are repressed by the action of four SIR gene products, most likely mediated through two cis-acting sites located on opposite sides of the locus. We showed that deletion of either of these two cis-acting sites from the chromosome di...

journal_title：Molecular and cellular biology

authors： Mahoney DJ,Broach JR

更新日期：1989-11-01 00:00:00

abstract：:The kidney's vital filtration function depends on the structural integrity of the glomerulus, the proximal portion of the nephron. Within the glomerulus, the architecturally complex podocyte forms the final cellular barrier to filtration. Injury to the podocyte results in a morphological change called foot process eff...

journal_title：Molecular and cellular biology

authors： Yu H,Suleiman H,Kim AH,Miner JH,Dani A,Shaw AS,Akilesh S

更新日期：2013-12-01 00:00:00

abstract：:The Saccharomyces cerevisiae Cdc42p GTPase interacts with multiple regulators and downstream effectors through an approximately 25-amino-acid effector domain. Four effector domain mutations, Y32K, F37A, D38E, and Y40C, were introduced into Cdc42p and characterized for their effects on these interactions. Each mutant p...

journal_title：Molecular and cellular biology

authors： Richman TJ,Johnson DI

更新日期：2000-11-01 00:00:00

abstract：:The short form (S1b) of the prolactin receptor (PRLR) silences prolactin-induced activation of gene transcription by the PRLR long form (LF). The functional and structural contributions of two intramolecular disulfide (S-S) bonds within the extracellular subdomain 1 (D1) of S1b to its inhibitory function on the LF wer...

journal_title：Molecular and cellular biology

authors： Xie YL,Hassan SA,Qazi AM,Tsai-Morris CH,Dufau ML

更新日期：2009-05-01 00:00:00

abstract：:We report in vivo analysis of histone and RNA polymerase II (pol II) occupancy at the 601 element, which functions as a strong in vitro nucleosome-positioning element and transcriptional pause site. Surprisingly, nucleosomes were not strongly positioned over the 601 element inserted either within a yeast chromosomal o...

journal_title：Molecular and cellular biology

authors： Perales R,Zhang L,Bentley D

更新日期：2011-08-01 00:00:00