RBM4 Regulates Neuronal Differentiation of Mesenchymal Stem Cells by Modulating Alternative Splicing of Pyruvate Kinase M.

abstract：:Two adjacent fragments of genomic DNA spanning the gene for CAD, which encodes the first three enzymes of UMP biosynthesis, were cloned from a mutant Syrian hamster cell line containing multiple copies of this gene. The mutant was selected for resistance to N-(phosphonacetyl)-L-aspartate, a potent and specific inhibit...

journal_title：Molecular and cellular biology

authors： Padgett RA,Wahl GM,Stark GR

更新日期：1982-03-01 00:00:00

abstract：:A pool of Candida albicans RsaI fragments cloned onto a vector containing pBR322 sequences and the Candida ADE2 gene was used to transform a Candida ade2 mutant to adenine protrophy. A potential autonomously replicating sequence (ARS) in Candida DNA was identified by two criteria: instability of the selectable marker ...

journal_title：Molecular and cellular biology

authors： Kurtz MB,Cortelyou MW,Miller SM,Lai M,Kirsch DR

更新日期：1987-01-01 00:00:00

abstract：:P bodies are cytoplasmic RNA granules containing the Dcp1-Dcp2 decapping enzymes where mRNA decay can occur. Here, we describe the characterization of P bodies in the fission yeast Schizosaccharomyces pombe. Most information on the property and function of P bodies stems from studies in the distantly related budding y...

journal_title：Molecular and cellular biology

authors： Wang CY,Chen WL,Wang SW

更新日期：2013-03-01 00:00:00

abstract：:Although mitogenic and differentiating factors often activate a number of common signaling pathways, the mechanisms leading to their distinct cellular outcomes have not been elucidated. In a previous report, we demonstrated that mitogen-activated protein (MAP) kinase (ERK) activation by the neurogenic agents fibroblas...

journal_title：Molecular and cellular biology

authors： Corbit KC,Soh JW,Yoshida K,Eves EM,Weinstein IB,Rosner MR

更新日期：2000-08-01 00:00:00

abstract：:The apoptotic process is accompanied by major changes in chromatin structure and gene expression. The apoptotic genetic program is progressively set up with the inhibition of antiapoptotic genes and the activation of proapoptotic ones. Here, we show that the histone deacetylase 3 (HDAC-3), which is a known co-represso...

journal_title：Molecular and cellular biology

authors： Escaffit F,Vaute O,Chevillard-Briet M,Segui B,Takami Y,Nakayama T,Trouche D

更新日期：2007-01-01 00:00:00

abstract：:The c-abl protooncogene is unusual in two respects; it has multiple, widely space N-terminal coding exons transcribed by different promoters, and it is the target of the translocations that form the Philadelphia chromosome found in cells of chronic myelogenous leukemia patients. To understand the organization of the g...

journal_title：Molecular and cellular biology

authors： Bernards A,Rubin CM,Westbrook CA,Paskind M,Baltimore D

更新日期：1987-09-01 00:00:00

abstract：:Okadaic acid-sensitive serine/threonine phosphatases have been shown to regulate interleukin-2 transcription and T-cell activation. Okadaic acid inhibits protein phosphatase 4 (PP4), a novel PP2A-related serine/threonine phosphatase, at a 50% inhibitory concentration (IC(50)) comparable to that for PP2A. This raises t...

journal_title：Molecular and cellular biology

authors： Shui JW,Hu MC,Tan TH

更新日期：2007-01-01 00:00:00

abstract：:The p53 tumor suppressor gene product is a sequence-specific DNA-binding protein that is necessary for the G1 arrest of many cell types. Consistent with its role as a cell cycle checkpoint factor, p53 has been shown to be capable of both transcriptional activation and repression. Here we show a new potential role for ...

journal_title：Molecular and cellular biology

authors： Miller SD,Farmer G,Prives C

更新日期：1995-12-01 00:00:00

abstract：:A significant challenge to our understanding of eukaryotic transcriptional regulation is to determine how multiple signal transduction pathways converge on a single promoter to regulate transcription in divergent fashions. To study this, we have investigated the transcriptional regulation of the Schizosaccharomyces po...

journal_title：Molecular and cellular biology

authors： Neely LA,Hoffman CS

更新日期：2000-09-01 00:00:00

abstract：:The adapter protein 3BP2 is expressed in lymphocytes; binds to Syk/ZAP-70, Vav, and phospholipase C-gamma (PLC-gamma); and is thought to be important for interleukin-2 gene transcription in T cells. To define the role of 3BP2 in lymphocyte development and function, we generated 3BP2-deficient mice. T-cell development,...

journal_title：Molecular and cellular biology

authors： de la Fuente MA,Kumar L,Lu B,Geha RS

更新日期：2006-07-01 00:00:00

abstract：:Using chromatin immunoprecipitation assays, we studied the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-mediated recruitment of the aryl hydrocarbon receptor (AhR) and several co-regulators to the CYP1A1 promoter. AhR displayed a time-dependent recruitment, reaching a peak at 75 min and maintaining promoter occupancy fo...

journal_title：Molecular and cellular biology

authors： Matthews J,Wihlén B,Thomsen J,Gustafsson JA

更新日期：2005-07-01 00:00:00

abstract：:The degradation of many proteins involves the sequential ligation of ubiquitin molecules to the substrate to form a multiubiquitin chain linked through Lys-48 of ubiquitin. To test for the existence of alternate forms of multiubiquitin chains, we examined the effects of individually substituting each of six other Lys ...

journal_title：Molecular and cellular biology

authors： Spence J,Sadis S,Haas AL,Finley D

更新日期：1995-03-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, sporulation occurs in response to nutritional and genetic signals. The process is initiated when nutrient availability limits mitotic growth, but only in MATa/MAT alpha diploid cells. Under these conditions, the cells express an activator of meiosis (IME1), which is required for ...

journal_title：Molecular and cellular biology

authors： Shah JC,Clancy MJ

更新日期：1992-03-01 00:00:00

abstract：:The short form (S1b) of the prolactin receptor (PRLR) silences prolactin-induced activation of gene transcription by the PRLR long form (LF). The functional and structural contributions of two intramolecular disulfide (S-S) bonds within the extracellular subdomain 1 (D1) of S1b to its inhibitory function on the LF wer...

journal_title：Molecular and cellular biology

authors： Xie YL,Hassan SA,Qazi AM,Tsai-Morris CH,Dufau ML

更新日期：2009-05-01 00:00:00

abstract：:Exchange proteins activated by cAMP (cyclic AMP) 2 (Epac2) is a guanine nucleotide exchange factor for Rap1, a small G protein involved in many cellular functions, including cell adhesion, differentiation, and exocytosis. Epac2 interacts with Ras-GTP via a Ras association (RA) domain. Previous studies have suggested t...

journal_title：Molecular and cellular biology

authors： Liu C,Takahashi M,Li Y,Song S,Dillon TJ,Shinde U,Stork PJ

更新日期：2008-12-01 00:00:00

abstract：:SLC5A8 is a putative tumor suppressor that is inactivated in more than 10 different types of cancer, but neither the oncogenic signaling responsible for SLC5A8 inactivation nor the functional relevance of SLC5A8 loss to tumor growth has been elucidated. Here, we identify oncogenic HRAS (HRAS(G12V)) as a potent mediato...

journal_title：Molecular and cellular biology

authors： Elangovan S,Pathania R,Ramachandran S,Ananth S,Padia RN,Srinivas SR,Babu E,Hawthorn L,Schoenlein PV,Boettger T,Smith SB,Prasad PD,Ganapathy V,Thangaraju M

更新日期：2013-10-01 00:00:00

abstract：:Upon the addition of different growth factors and cytokines, the Gab1 docking protein is tyrosine phosphorylated and in turn activates different signaling pathways. On the basis of the large body of evidence concerning cross talk between the signaling pathways activated by growth factors and oxidative stress, we decid...

journal_title：Molecular and cellular biology

authors： Holgado-Madruga M,Wong AJ

更新日期：2003-07-01 00:00:00

abstract：:The int-1 proto-oncogene is a target for insertional activation of transcription by mouse mammary tumor virus in many murine mammary tumors. Whereas no expression of int-1 is seen in normal mammary tissue, int-1 RNA can be detected in normal mice in the neural tubes of midgestation embryos and in postmeiotic spermatoc...

journal_title：Molecular and cellular biology

authors： Papkoff J

更新日期：1989-08-01 00:00:00

abstract：:In Aspergillus nidulans, a fungus that possesses negligible, if any, levels of methylation in its genome, low concentrations of 5-azacytidine (5-AC) convert a high percentage of the cell population to fluffy phenotypic variants through a heritable modification of a single nuclear gene (M. Tamame, F. Antequera, J. R. V...

journal_title：Molecular and cellular biology

authors： Tamame M,Antequera F,Santos E

更新日期：1988-08-01 00:00:00

abstract：:The c-myc proto-oncogene encodes a transcription factor that participates in the regulation of cellular proliferation, differentiation, and apoptosis. Ectopic overexpression of c-Myc has been shown to sensitize cells to apoptosis. We report here that cells lacking c-Myc activity due to disruption of the c-myc gene by ...

journal_title：Molecular and cellular biology

authors： Adachi S,Obaya AJ,Han Z,Ramos-Desimone N,Wyche JH,Sedivy JM

更新日期：2001-08-01 00:00:00

abstract：:Processing from pre-mRNA introns is a widespread mechanism to generate human box C/D and H/ACA snoRNAs. Recent studies revealed that an optimal position relative to the 3' splice site is important for efficient processing of most box C/D snoRNAs and that assembly of box C/D snoRNPs is stimulated by splicing factors li...

journal_title：Molecular and cellular biology

authors： Richard P,Kiss AM,Darzacq X,Kiss T

更新日期：2006-04-01 00:00:00

abstract：:Tissue-specific gene expression requires modulation of nucleosomes, allowing transcription factors to occupy cis elements that are accessible only in selected tissues. Master transcription factors control cell-specific genes and define cellular identities, but it is unclear if they possess special abilities to regulat...

journal_title：Molecular and cellular biology

authors： Verzi MP,Shin H,San Roman AK,Liu XS,Shivdasani RA

更新日期：2013-01-01 00:00:00

abstract：:The Capn5 gene was inactivated by homologous recombination in ES cells that subsequently colonized the germ line of mice. The targeted mutation integrated a lacZ expression cassette into the Capn5 gene, allowing the expression of Capn5 mRNA to be examined in detail in heterozygous animals. Expression was observed in e...

journal_title：Molecular and cellular biology

authors： Franz T,Winckler L,Boehm T,Dear TN

更新日期：2004-02-01 00:00:00

abstract：:To interrogate endogenous p21(WAF1/CIP1) (p21) promoter activity under basal conditions and in response to various forms of stress, knock-in imaging reporter mice in which expression of firefly luciferase (FLuc) was placed under the control of the endogenous p21 promoter within the Cdkn1a gene locus were generated. Bi...

journal_title：Molecular and cellular biology

authors： Tinkum KL,Marpegan L,White LS,Sun J,Herzog ED,Piwnica-Worms D,Piwnica-Worms H

更新日期：2011-09-01 00:00:00

abstract：:Recently, we demonstrated that the microRNA 424(322)/503 [miR-424(322)/503] cluster is transcriptionally controlled by transforming growth factor β (TGF-β) in the mammary epithelium. Induction of this microRNA cluster impacts mammary epithelium fate by regulating apoptosis and insulin-like growth factor 1 (IGF1) signa...

journal_title：Molecular and cellular biology

authors： Llobet-Navas D,Rodriguez-Barrueco R,de la Iglesia-Vicente J,Olivan M,Castro V,Saucedo-Cuevas L,Marshall N,Putcha P,Castillo-Martin M,Bardot E,Ezhkova E,Iavarone A,Cordon-Cardo C,Silva JM

更新日期：2014-12-01 00:00:00

abstract：:Fred Sherman was a prominent yeast geneticist and my mentor in graduate school. Fred passed away in September 2013 at the age of 81. In this minireview, I describe what it was like to know Fred and be in his lab from 1977 to 1982, the extraordinarily exciting time when the recombinant DNA revolution hit yeast genetics...

journal_title：Molecular and cellular biology

authors： Zaret KS

更新日期：2014-03-01 00:00:00

abstract：:The extensive array of basic helix-loop-helix (bHLH) transcription factors and their combinations as dimers underpin the diversity of molecular function required for cell type specification during embryogenesis. The bHLH factor TWIST1 plays pleiotropic roles during development. However, which combinations of TWIST1 di...

journal_title：Molecular and cellular biology

authors： Fan X,Waardenberg AJ,Demuth M,Osteil P,Sun JQJ,Loebel DAF,Graham M,Tam PPL,Fossat N

更新日期：2020-05-14 00:00:00

abstract：:The yeast nuclear gene YME1 was one of six genes recently identified in a screen for mutations that elevate the rate at which DNA escapes from mitochondria and migrates to the nucleus. yme1 mutations, including a deletion, cause four known recessive phenotypes: an elevation in the rate at which copies of TRP1 and ARS1...

journal_title：Molecular and cellular biology

authors： Thorsness PE,White KH,Fox TD

更新日期：1993-09-01 00:00:00

abstract：:We have isolated a yeast conditional mutant which rapidly ceases synthesis of mRNA when subjected to the nonpermissive temperature. This mutant (rpb1-1) was constructed by replacing the wild-type chromosomal copy of the gene encoding the largest subunit of RNA polymerase II with one mutagenized in vitro. The rapid ces...

journal_title：Molecular and cellular biology

authors： Nonet M,Scafe C,Sexton J,Young R

更新日期：1987-05-01 00:00:00

abstract：:Using a Saccharomyces cerevisiae strain containing an integrated copy of an H2A-lacZ fusion gene, we screened for mutants which overexpressed beta-galactosidase as a way to identify genes which regulate transcription of the histone genes. Five recessive mutants with this phenotype were shown to contain altered regulat...

journal_title：Molecular and cellular biology

authors： Osley MA,Lycan D

更新日期：1987-12-01 00:00:00