Abstract:
:Recently, we demonstrated that the microRNA 424(322)/503 [miR-424(322)/503] cluster is transcriptionally controlled by transforming growth factor β (TGF-β) in the mammary epithelium. Induction of this microRNA cluster impacts mammary epithelium fate by regulating apoptosis and insulin-like growth factor 1 (IGF1) signaling. Here, we expanded our finding to demonstrate that miR-424(322)/503 is an integral component of the cell cycle arrest mediated by TGF-β. Mechanistically, we showed that after TGF-β exposure, increased levels of miR-424(322)/503 reduce the expression of the cell cycle regulator CDC25A. miR-424(322)/503-dependent posttranscriptional downregulation of CDC25A cooperates with previously described transcriptional repression of the CDC25A promoter and proteasome-mediated degradation to reduce the levels of CDC25A expression and to induce cell cycle arrest. We also provide evidence that the TGF-β/miR-424(322)/503 axis is part of the mechanism that regulates the proliferation of hormone receptor-positive (HR(+)) mammary epithelial cells in vivo.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Llobet-Navas D,Rodriguez-Barrueco R,de la Iglesia-Vicente J,Olivan M,Castro V,Saucedo-Cuevas L,Marshall N,Putcha P,Castillo-Martin M,Bardot E,Ezhkova E,Iavarone A,Cordon-Cardo C,Silva JMdoi
10.1128/MCB.00611-14subject
Has Abstractpub_date
2014-12-01 00:00:00pages
4216-31issue
23eissn
0270-7306issn
1098-5549pii
MCB.00611-14journal_volume
34pub_type
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