Abstract:
:Notch signaling commences with two ligand-mediated proteolysis events that release the Notch intracellular domain, NICD, from the plasma membrane. NICD then translocates into the nucleus and interacts with the DNA binding protein CSL to activate transcription. We found that NICD expression also potentiates activity of the transcription factor LEF-1. NICD stimulation of LEF-1 activity was context dependent and occurred on a subset of promoters distinct from those activated by beta-catenin. Importantly, the effect of NICD does not appear to be mediated through canonical components of the Wnt signaling pathway or downstream components of the Notch pathway. In vitro assays show a weak association between the C-terminal transactivation domain of NICD and the high-mobility group domain of LEF-1, suggesting that the two proteins interact in vivo. Our data therefore describe a new nuclear target of Notch signaling and a new coactivator for LEF-1.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Ross DA,Kadesch Tdoi
10.1128/MCB.21.22.7537-7544.2001subject
Has Abstractpub_date
2001-11-01 00:00:00pages
7537-44issue
22eissn
0270-7306issn
1098-5549journal_volume
21pub_type
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