Abstract:
:The Saccharomyces cerevisiae PGK (phosphoglycerate kinase) gene encodes one of the most abundant mRNA and protein species in the cell. To identify the promoter sequences required for the efficient expression of PGK, we undertook a detailed internal deletion analysis of the 5' noncoding region of the gene. Our analysis revealed that PGK has an upstream activation sequence (UASPGK) located between 402 and 479 nucleotides upstream from the initiating ATG sequence which is required for full transcriptional activity. Deletion of this sequence caused a marked reduction in the levels of PGK transcription. We showed that PGK has no requirement for TATA sequences; deletion of one or both potential TATA sequences had no effect on either the levels of PGK expression or the accuracy of transcription initiation. We also showed that the UASPGK functions as efficiently when in the inverted orientation and that it can enhance transcription when placed upstream of a TRP1-IFN fusion gene comprising the promoter of TRP1 fused to the coding region of human interferon alpha-2.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Ogden JE,Stanway C,Kim S,Mellor J,Kingsman AJ,Kingsman SMdoi
10.1128/mcb.6.12.4335subject
Has Abstractpub_date
1986-12-01 00:00:00pages
4335-43issue
12eissn
0270-7306issn
1098-5549journal_volume
6pub_type
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