A nuclear factor (NF2d9) that binds to the male-specific P450 (Cyp 2d-9) gene in mouse liver.

abstract：:We analyzed epsilon-globin transcription in erythroid cells and in erythroid extracts to determine the requirements for enhancer-dependent expression of this gene. Mutations that abolished GATA-1 binding at a single position in the promoter prevented interaction with enhancers, whereas elimination of a second more dis...

journal_title：Molecular and cellular biology

authors： Gong Q,Dean A

更新日期：1993-02-01 00:00:00

abstract：:We provide evidence that human SLFN5, an interferon (IFN)-inducible member of the Schlafen (SLFN) family of proteins, exhibits key roles in controlling motility and invasiveness of renal cell carcinoma (RCC) cells. Our studies define the mechanism by which this occurs, demonstrating that SLFN5 negatively controls expr...

journal_title：Molecular and cellular biology

authors： Sassano A,Mavrommatis E,Arslan AD,Kroczynska B,Beauchamp EM,Khuon S,Chew TL,Green KJ,Munshi HG,Verma AK,Platanias LC

更新日期：2015-08-01 00:00:00

abstract：:The ribonucleoprotein enzyme telomerase is a specialized reverse transcriptase that synthesizes telomeric DNA by copying a template sequence within the telomerase RNA. Here we analyze the actions of telomerase from Tetrahymena thermophila assembled in vivo with mutated or wild-type telomerase RNA to define further the...

journal_title：Molecular and cellular biology

authors： Gilley D,Blackburn EH

更新日期：1996-01-01 00:00:00

abstract：:Posttranslational modification of general transcription factors may be an important mechanism for global gene regulation. The general transcription factor IIA (TFIIA) binds to the TATA binding protein (TBP) and is essential for high-level transcription mediated by various activators. Modulation of the TFIIA-TBP intera...

journal_title：Molecular and cellular biology

authors： Solow SP,Lezina L,Lieberman PM

更新日期：1999-04-01 00:00:00

abstract：:Mouse neuroblastoma Neuro-2A cells produce transforming growth factors during exponential growth in a defined hormone-free medium, which, on Bio-Gel columns in 1 M HAc, elute at a molecular size of 15 to 20 kilodaltons (kDa). These neuroblastoma-derived transforming growth factors have strong mitogenic activity, but t...

journal_title：Molecular and cellular biology

authors： van Zoelen EJ,van de Ven WJ,Franssen HJ,van Oostwaard TM,van der Saag PT,Heldin CH,de Laat SW

更新日期：1985-09-01 00:00:00

abstract：:Proteins encoded by the mdm2 gene, which has a pivotal role in the regulation of growth and differentiation, exist principally in human and murine cells as two isoforms that migrate in gels as 75-kDa and 90-kDa proteins. There is limited understanding of the respective biological roles of these isoforms, their molecul...

journal_title：Molecular and cellular biology

authors： Cheng TH,Cohen SN

更新日期：2007-01-01 00:00:00

abstract：:Glutamine is the preferred source of nitrogen of Neurospora crassa. In its presence and that of the gene product of MS5 (nmr-1), the fungus represses the assimilation of less preferred forms of nitrogen, such as nitrate. In the absence of glutamine and the presence of the product of gene nit-2, less preferred forms of...

journal_title：Molecular and cellular biology

authors： Sorger GJ,Brown D,Farzannejad M,Guerra A,Jonathan M,Knight S,Sharda R

更新日期：1989-09-01 00:00:00

abstract：:We have identified stable transcripts from the so-called nontranscribed spacer region (NTS) of the nuclear ribosomal DNA repeat in certain respiration-deficient strains of Saccharomyces cerevisiae. These RNAs, which are transcribed from the same strand as is the 37S rRNA precursor, are 500 to 800 nucleotides long and ...

journal_title：Molecular and cellular biology

authors： Parikh VS,Conrad-Webb H,Docherty R,Butow RA

更新日期：1989-05-01 00:00:00

abstract：:The nuclear receptor steroidogenic factor 1 (SF-1) is essential for adrenal development and steroidogenesis. The atypical orphan nuclear receptor Dax-1 binds to SF-1 and represses SF-1 target genes. Paradoxically, however, loss-of-function mutations of Dax-1 also cause adrenal hypoplasia, suggesting that Dax-1 may fun...

journal_title：Molecular and cellular biology

authors： Xu B,Yang WH,Gerin I,Hu CD,Hammer GD,Koenig RJ

更新日期：2009-04-01 00:00:00

abstract：:The vitamin D receptor (VDR) normally functions as a ligand-dependent transcriptional activator. Here we show that, in the presence of Ets-1, VDR stimulates the prolactin promoter in a ligand-independent manner, behaving as a constitutive activator. Mutations in the AF2 domain abolish vitamin D-dependent transactivati...

journal_title：Molecular and cellular biology

authors： Tolón RM,Castillo AI,Jiménez-Lara AM,Aranda A

更新日期：2000-12-01 00:00:00

abstract：:The polycomb protein Bmi-1 represses the INK4a locus, which encodes the tumor suppressors p16 and p14(ARF). Here we report that Bmi-1 is downregulated when WI-38 human fibroblasts undergo replicative senescence, but not quiescence, and extends replicative life span when overexpressed. Life span extension by Bmi-1 requ...

journal_title：Molecular and cellular biology

authors： Itahana K,Zou Y,Itahana Y,Martinez JL,Beausejour C,Jacobs JJ,Van Lohuizen M,Band V,Campisi J,Dimri GP

更新日期：2003-01-01 00:00:00

abstract：:Unique single-stranded regions of simian virus 40 DNA, phage M13 virion DNA, and several homopolymers were used as templates for the synthesis of (p)ppRNA-DNA chains by CV-1 cell DNA primase-DNA polymerase alpha. Intact RNA primers, specifically labeled with an RNA capping enzyme, were typically 6 to 8 ribonucleotides...

journal_title：Molecular and cellular biology

authors： Yamaguchi M,Hendrickson EA,DePamphilis ML

更新日期：1985-05-01 00:00:00

abstract：:We have characterized the role of YPR128cp, the orthologue of human PMP34, in fatty acid metabolism and peroxisomal proliferation in Saccharomyces cerevisiae. YPR128cp belongs to the mitochondrial carrier family (MCF) of solute transporters and is localized in the peroxisomal membrane. Disruption of the YPR128c gene r...

journal_title：Molecular and cellular biology

authors： van Roermund CW,Drissen R,van Den Berg M,Ijlst L,Hettema EH,Tabak HF,Waterham HR,Wanders RJ

更新日期：2001-07-01 00:00:00

abstract：:Determining the functions of novel genes implicated in cell survival is directly relevant to our understanding of mammalian development and carcinogenesis. ARS2 is an evolutionarily conserved gene that confers arsenite resistance on arsenite-sensitive Chinese hamster ovary cells. Little is known regarding the function...

journal_title：Molecular and cellular biology

authors： Wilson MD,Wang D,Wagner R,Breyssens H,Gertsenstein M,Lobe C,Lu X,Nagy A,Burke RD,Koop BF,Howard PL

更新日期：2008-03-01 00:00:00

abstract：:The autonomously replicating rRNA genes (rDNA) in the somatic nucleus of Tetrahymena thermophila are maintained at a copy number of approximately 10(4) per nucleus. A mutant in which the replication properties of this molecule were altered was isolated and characterized. This mutation of inbred strain C3, named rmm4, ...

journal_title：Molecular and cellular biology

authors： Yaeger PC,Orias E,Shaiu WL,Larson DD,Blackburn EH

更新日期：1989-02-01 00:00:00

abstract：:Numb proteins are evolutionarily conserved signaling molecules that make the daughter cells different after asymmetric divisions by segregating to only one daughter. They contain distinct binding motifs for alpha-adaptin (alpha-Ada) and proteins with Eps15 homology (EH) domains, which regulate endocytosis, and for E3 ...

journal_title：Molecular and cellular biology

authors： Tang H,Rompani SB,Atkins JB,Zhou Y,Osterwalder T,Zhong W

更新日期：2005-04-01 00:00:00

abstract：:The DNA-binding transcriptional activator Gal4 and its regulators Gal80 and Gal3 constitute a galactose-responsive switch for the GAL genes of Saccharomyces cerevisiae. Gal4 binds to GAL gene UASGAL (upstream activation sequence in GAL gene promoter) sites as a dimer via its N-terminal domain and activates transcripti...

journal_title：Molecular and cellular biology

authors： Egriboz O,Goswami S,Tao X,Dotts K,Schaeffer C,Pilauri V,Hopper JE

更新日期：2013-09-01 00:00:00

abstract：:We have exploited the Escherichia coli lac operator/repressor system as a means to regulate the expression of a mammalian tRNA gene in vivo and in vitro. An oligonucleotide containing a lac operator (lacO) site was cloned immediately upstream of a human serine amber suppressor (Su+) tRNA gene. Insertion of a single la...

journal_title：Molecular and cellular biology

authors： Syroid DE,Tapping RI,Capone JP

更新日期：1992-10-01 00:00:00

abstract：:There are five members of the RFX family of transcription factors in mammals. While RFX5 plays a well-defined role in the immune system, the functions of RFX1 to RFX4 remain largely unknown. We have generated mice with a deletion of the Rfx3 gene. RFX3-deficient mice exhibit frequent left-right (LR) asymmetry defects ...

journal_title：Molecular and cellular biology

authors： Bonnafe E,Touka M,AitLounis A,Baas D,Barras E,Ucla C,Moreau A,Flamant F,Dubruille R,Couble P,Collignon J,Durand B,Reith W

更新日期：2004-05-01 00:00:00

abstract：:We have designed and constructed integration-defective retroviral vectors to explore their potential for gene targeting in mammalian cells. Two nonoverlapping deletion mutants of the bacterial neomycin resistance (neo) gene were used to detect homologous recombination events between viral and chromosomal sequences. St...

journal_title：Molecular and cellular biology

authors： Ellis J,Bernstein A

更新日期：1989-04-01 00:00:00

abstract：:Bright/Arid3a has been characterized both as an activator of immunoglobulin heavy-chain transcription and as a proto-oncogene. Although Bright expression is highly B lineage stage restricted in adult mice, its expression in the earliest identifiable hematopoietic stem cell (HSC) population suggests that Bright might h...

journal_title：Molecular and cellular biology

authors： Webb CF,Bryant J,Popowski M,Allred L,Kim D,Harriss J,Schmidt C,Miner CA,Rose K,Cheng HL,Griffin C,Tucker PW

更新日期：2011-03-01 00:00:00

abstract：:Budding yeast polo kinase Cdc5p localizes to the spindle pole body (SPB) and to the bud-neck and plays multiple roles during M-phase progression. To dissect localization-specific mitotic functions of Cdc5p, we tethered a localization-defective N-terminal kinase domain of Cdc5p (Cdc5pDeltaC) to the SPB or to the bud-ne...

journal_title：Molecular and cellular biology

authors： Park JE,Park CJ,Sakchaisri K,Karpova T,Asano S,McNally J,Sunwoo Y,Leem SH,Lee KS

更新日期：2004-11-01 00:00:00

abstract：:Selenoprotein mRNAs are potential targets for degradation via nonsense-mediated decay due to the presence of in-frame UGA codons that can be decoded as either selenocysteine or termination codons. When UGA decoding is inefficient, as occurs when selenium is limiting, termination occurs at these positions. Based on the...

journal_title：Molecular and cellular biology

authors： Squires JE,Stoytchev I,Forry EP,Berry MJ

更新日期：2007-11-01 00:00:00

abstract：:Brain disturbances, like injuries or aberrant protein deposits, evoke nucleotide release or leakage from cells, leading to microglial chemotaxis and ingestion. Recent studies have identified P2Y12 purinergic receptors as triggers for microglial chemotaxis and P2Y6 receptors as mediators for phagocytosis. However, pino...

journal_title：Molecular and cellular biology

authors： Li HQ,Chen C,Dou Y,Wu HJ,Liu YJ,Lou HF,Zhang JM,Li XM,Wang H,Duan S

更新日期：2013-11-01 00:00:00

abstract：:During development and erythropoiesis, globin gene expression is finely modulated through an important network of transcription factors and chromatin modifying activities. In this report we provide in vivo evidence that endogenous Ikaros is recruited to the human beta-globin locus and targets the histone deacetylase H...

journal_title：Molecular and cellular biology

authors： Bottardi S,Ross J,Bourgoin V,Fotouhi-Ardakani N,Affar el B,Trudel M,Milot E

更新日期：2009-03-01 00:00:00

abstract：:We introduced a human beta-globin gene into murine erythroleukemia (MEL) cells by infection with recombinant retroviruses containing the complete genomic globin sequence. The beta-globin gene was correctly regulated during differentiation, steady-state mRNA levels being induced 5- to 30-fold after treatment of the cel...

journal_title：Molecular and cellular biology

authors： Cone RD,Weber-Benarous A,Baorto D,Mulligan RC

更新日期：1987-02-01 00:00:00

abstract：:We have developed a high-frequency cotransformation system which is useful in introducing nonreplicating circular DNA plasmids into the fission yeast Schizosaccharomyces pombe. This system depends on two factors: the ability of the ural-complementing helper plasmids pFYM2 and pFYM225 to propagate autonomously in S. po...

journal_title：Molecular and cellular biology

authors： Sakai K,Sakaguchi J,Yamamoto M

更新日期：1984-04-01 00:00:00

abstract：:Mdm2, a regulator of the tumor suppressor p53, is frequently overexpressed in human malignancies. Mdm2 also has unresolved, p53-independent functions that contribute to tumorigenesis. Here, we show that increased Mdm2 expression induced chromosome/chromatid breaks and delayed DNA double-strand break repair in cells la...

journal_title：Molecular and cellular biology

authors： Bouska A,Lushnikova T,Plaza S,Eischen CM

更新日期：2008-08-01 00:00:00

abstract：:Imprinted genes are expressed in a monoallelic, parent-of-origin-specific manner. Clusters of imprinted genes are regulated by imprinting control regions (ICRs) characterized by DNA methylation of one allele. This methylation is critical for imprinting; a reduction in the DNA methyltransferase DNMT1 causes a widesprea...

journal_title：Molecular and cellular biology

authors： Weaver JR,Sarkisian G,Krapp C,Mager J,Mann MR,Bartolomei MS

更新日期：2010-08-01 00:00:00

abstract：:Ret/ptc2 is a constitutively active, oncogenic form of the c-Ret receptor tyrosine kinase. Like the other papillary thyroid carcinoma forms of Ret, Ret/ptc2 is activated through fusion of the Ret tyrosine kinase domain to the dimerization domain of another protein. Investigation of requirements for Ret/ptc2 mitogenic ...

journal_title：Molecular and cellular biology

authors： Durick K,Gill GN,Taylor SS

更新日期：1998-04-01 00:00:00