Identification of a peroxisomal ATP carrier required for medium-chain fatty acid beta-oxidation and normal peroxisome proliferation in Saccharomyces cerevisiae.

abstract：:Our objective was to establish an experimental model of a self-sustained and bistable extracellular signal-regulated kinase 1/2 (ERK1/2) signaling process. A single stimulation of cells with cytokines causes rapid ERK1/2 activation, which returns to baseline in 4 h. Repeated stimulation leads to sustained activation o...

journal_title：Molecular and cellular biology

authors： Liu W,Tundwal K,Liang Q,Goplen N,Rozario S,Quayum N,Gorska M,Wenzel S,Balzar S,Alam R

更新日期：2010-04-01 00:00:00

abstract：:The role of chromatin-remodeling factors in transcription is well established, but the link between chromatin-remodeling complexes and DNA repair remains unexplored. Human Rvb1 and Rvb2 are highly conserved AAA(+) ATP binding proteins that are part of various chromatin-remodeling complexes, such as Ino80, SNF2-related...

journal_title：Molecular and cellular biology

authors： Jha S,Shibata E,Dutta A

更新日期：2008-04-01 00:00:00

abstract：:Signal transduction by antigen receptors and some Fc receptors requires the activation of a family of receptor-associated transmembrane accessory proteins. One common feature of the cytoplasmic domains of these accessory molecules is the presence is at least two YXXA repeats that are potential sites for interaction wi...

journal_title：Molecular and cellular biology

authors： Burkhardt AL,Costa T,Misulovin Z,Stealy B,Bolen JB,Nussenzweig MC

更新日期：1994-02-01 00:00:00

abstract：:The functional importance of the two clusters of positively charged amino acids which flank the hydrophobic membrane-anchoring sequence of polyomavirus middle T (mT) protein has been investigated by using site-directed mutagenesis. A clear asymmetry was apparent. No effect on transformation was seen following multiple...

journal_title：Molecular and cellular biology

authors： Dahl J,Thathamangalam U,Freund R,Benjamin TL

更新日期：1992-11-01 00:00:00

abstract：:To examine a role for focal adhesion kinase (FAK) in cardiac morphogenesis, we generated a line of mice with a conditional deletion of FAK in nkx2-5-expressing cells (herein termed FAKnk mice). FAKnk mice died shortly after birth, likely resulting from a profound subaortic ventricular septal defect and associated mala...

journal_title：Molecular and cellular biology

authors： Hakim ZS,DiMichele LA,Doherty JT,Homeister JW,Beggs HE,Reichardt LF,Schwartz RJ,Brackhan J,Smithies O,Mack CP,Taylor JM

更新日期：2007-08-01 00:00:00

abstract：:Mediator is a general coactivator complex connecting transcription activators and RNA polymerase II. Recent work has shown that the nuclear receptor-interacting MED1/TRAP220 subunit of Mediator is required for peroxisome proliferator-activated receptor gamma (PPARgamma)-stimulated adipogenesis of mouse embryonic fibro...

journal_title：Molecular and cellular biology

authors： Ge K,Cho YW,Guo H,Hong TB,Guermah M,Ito M,Yu H,Kalkum M,Roeder RG

更新日期：2008-02-01 00:00:00

abstract：:We demonstrate that the immunoglobulin heavy-chain enhancer functions as the promoter for I mu sterile transcription. The enhancer itself, when placed 5' to the bacterial cat or neo genes, is able to direct transcription by using heterogeneous start sites that are generally the same as those found with bona fide I mu ...

journal_title：Molecular and cellular biology

authors： Su LK,Kadesch T

更新日期：1990-06-01 00:00:00

abstract：:Heterogeneous nuclear RNA contains double-stranded regions that are not found in mRNA and that may serve as recognition elements for processing enzymes. The double-stranded regions of heterogeneous nuclear RNA prepared from HeLa cells promoted the synthesis of (2',5')oligoadenylate [(2',5')oligo(A) or (2'5')An] when i...

journal_title：Molecular and cellular biology

authors： Nilsen TW,Maroney PA,Robertson HD,Baglioni C

更新日期：1982-02-01 00:00:00

abstract：:Mdm2 and MdmX are structurally related p53-binding proteins that function as critical negative regulators of p53 activity in embryonic and adult tissue. The overexpression of Mdm2 or MdmX inhibits p53 tumor suppressor functions in vitro, and the amplification of Mdm2 or MdmX is observed in human cancers retaining wild...

journal_title：Molecular and cellular biology

authors： Matijasevic Z,Steinman HA,Hoover K,Jones SN

更新日期：2008-02-01 00:00:00

abstract：:The RNA-binding protein (RBP) nucleolin promotes the expression of several proliferative proteins. Nucleolin levels are high in cancer cells, but the mechanisms that control nucleolin expression are unknown. Here, we show that nucleolin abundance is controlled posttranscriptionally via factors that associate with its ...

journal_title：Molecular and cellular biology

authors： Tominaga K,Srikantan S,Lee EK,Subaran SS,Martindale JL,Abdelmohsen K,Gorospe M

更新日期：2011-10-01 00:00:00

abstract：:The interferon (IFN)-induced double-stranded RNA-activated protein kinase PKR mediates inhibition of protein synthesis through phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2alpha) and is also involved in the induction of the IFN gene through the activation of the transcription factor NF-k...

journal_title：Molecular and cellular biology

authors： Bonnet MC,Weil R,Dam E,Hovanessian AG,Meurs EF

更新日期：2000-07-01 00:00:00

abstract：:DHTKD1, a part of 2-ketoadipic acid dehydrogenase complex, is involved in lysine and tryptophan catabolism. Mutations in DHTKD1 block the metabolic pathway and cause 2-aminoadipic and 2-oxoadipic aciduria (AMOXAD), an autosomal recessive inborn metabolic disorder. In addition, a nonsense mutation in DHTKD1 that we ide...

journal_title：Molecular and cellular biology

authors： Xu WY,Zhu H,Shen Y,Wan YH,Tu XD,Wu WT,Tang L,Zhang HX,Lu SY,Jin XL,Fei J,Wang ZG

更新日期：2018-06-14 00:00:00

abstract：:The cytosolic heat shock cognate 70-kDa protein (hsc70) is required for efficient import of ornithine transcarbamylase precursor (pOTC) into rat liver mitochondria (K. Terada, K. Ohtsuka, N. Imamoto, Y. Yoneda, and M. Mori, Mol. Cell. Biol. 15:3708-3713, 1995). The requirement of hsc70 for mitochondrial import of vari...

journal_title：Molecular and cellular biology

authors： Terada K,Ueda I,Ohtsuka K,Oda T,Ichiyama A,Mori M

更新日期：1996-11-01 00:00:00

abstract：:R-loops have been described in vivo at the immunoglobulin class switch sequences and at prokaryotic and mitochondrial origins of replication. However, the biochemical mechanism and determinants of R-loop formation are unclear. We find that R-loop formation is nearly eliminated when RNase T(1) is added during transcrip...

journal_title：Molecular and cellular biology

authors： Roy D,Yu K,Lieber MR

更新日期：2008-01-01 00:00:00

abstract：:Even though endothelin is recognized as an important vasoregulatory molecule, the roles of endothelin receptors in specific cell types are not yet fully understood. Mice with a null mutation in endothelin A receptor gene (ET(A)) or in the gene of its ligand (endothelin 1) die neonatally due to craniofacial and cardiac...

journal_title：Molecular and cellular biology

authors： Kedzierski RM,Grayburn PA,Kisanuki YY,Williams CS,Hammer RE,Richardson JA,Schneider MD,Yanagisawa M

更新日期：2003-11-01 00:00:00

abstract：:The c-ABL tyrosine kinase is activated following either the loss or mutation of its Src homology domain 3 (SH3), resulting in both increased autophosphorylation and phosphorylation of cellular substrates and cellular transformation. This suggests that the SH3 domain negatively regulates c-ABL kinase activity. For seve...

journal_title：Molecular and cellular biology

authors： Zhu J,Shore SK

更新日期：1996-12-01 00:00:00

abstract：:Normal cells do not divide indefinitely due to a process known as replicative senescence. Human cells arrest growth with a senescent phenotype when they acquire one or more critically short telomeres as a consequence of cell division. Recent evidence suggests that certain types of DNA damage, chromatin remodeling, and...

journal_title：Molecular and cellular biology

authors： Dimri GP,Itahana K,Acosta M,Campisi J

更新日期：2000-01-01 00:00:00

abstract：:COUP-TF, an orphan member of the nuclear receptor superfamily, has been proposed to play a key role in regulating organogenesis, neurogenesis, and cellular differentiation during embryonic development. Since heterodimierization is a common theme within the nuclear receptor superfamily and has been demonstrated to modu...

journal_title：Molecular and cellular biology

authors： Leng X,Cooney AJ,Tsai SY,Tsai MJ

更新日期：1996-05-01 00:00:00

abstract：:Two alpha-tubulin genes from the budding yeast Saccharomyces cerevisiae were identified and cloned by cross-species DNA homology. Nucleotide sequencing studies revealed that the two genes, named TUB1 and TUB3, encoded gene products of 447 and 445 amino acids, respectively, that are highly homologous to alpha-tubulins ...

journal_title：Molecular and cellular biology

authors： Schatz PJ,Pillus L,Grisafi P,Solomon F,Botstein D

更新日期：1986-11-01 00:00:00

abstract：:Genetic and biochemical evidence implicates chromatin structure in the silencing of the two quiescent mating-type loci near the telomeres of chromosome III in yeast. With high-resolution micrococcal nuclease mapping, we show that the HMRa locus has 12 precisely positioned nucleosomes spanning the distance between the ...

journal_title：Molecular and cellular biology

authors： Ravindra A,Weiss K,Simpson RT

更新日期：1999-12-01 00:00:00

abstract：:The chimeric oncoprotein E2A-HLF, generated by the t(17;19) chromosomal translocation in pro-B-cell acute lymphoblastic leukemia, incorporates the transactivation domains of E2A and the basic leucine zipper (bZIP) DNA-binding and protein dimerization domain of HLF (hepatic leukemic factor). The ability of E2A-HLF to p...

journal_title：Molecular and cellular biology

authors： Inukai T,Inaba T,Ikushima S,Look AT

更新日期：1998-10-01 00:00:00

abstract：:Nonhistone chromosomal proteins HMG-14 and HMG-17 are closely related nucleosomal binding proteins that unfold the higher-order chromatin structure, thereby enhancing the transcription and replication potential of chromatin. Here we report that PCAF, a transcription coactivator with intrinsic histone acetyltransferase...

journal_title：Molecular and cellular biology

authors： Herrera JE,Sakaguchi K,Bergel M,Trieschmann L,Nakatani Y,Bustin M

更新日期：1999-05-01 00:00:00

abstract：:Telomere maintenance is required for chromosome stability, and telomeres are typically replicated by the action of the reverse transcriptase telomerase. In both tumor and yeast cells that lack telomerase, telomeres are maintained by an alternative recombination mechanism. Genetic studies have led to the identification...

journal_title：Molecular and cellular biology

authors： Tsai YL,Tseng SF,Chang SH,Lin CC,Teng SC

更新日期：2002-08-01 00:00:00

abstract：:The let-23 gene encodes a Caenorhabditis elegans homolog of the epidermal growth factor receptor (EGFR) necessary for vulval development. We have characterized a mutation of let-23 that activates the receptor and downstream signal transduction, leading to excess vulval differentiation. This mutation alters a conserved...

journal_title：Molecular and cellular biology

authors： Katz WS,Lesa GM,Yannoukakos D,Clandinin TR,Schlessinger J,Sternberg PW

更新日期：1996-02-01 00:00:00

abstract：:Budding yeast Rad53 is an essential protein kinase that is phosphorylated and activated in a MEC1- and TEL1-dependent manner in response to DNA damage. We studied the role of Rad53 phosphorylation through mutation of consensus phosphorylation sites for upstream kinases Mec1 and Tel1. Alanine substitution of the Rad53 ...

journal_title：Molecular and cellular biology

authors： Lee SJ,Schwartz MF,Duong JK,Stern DF

更新日期：2003-09-01 00:00:00

abstract：:Ewing's family tumor (EFT) is a rare pediatric tumor of unclear origin that occurs in bone and soft tissue. Specific chromosomal translocations found in EFT cause EWS to fuse to a subset of ets transcription factor genes (ETS), generating chimeric EWS/ETS proteins. These proteins are believed to play a crucial role in...

journal_title：Molecular and cellular biology

authors： Miyagawa Y,Okita H,Nakaijima H,Horiuchi Y,Sato B,Taguchi T,Toyoda M,Katagiri YU,Fujimoto J,Hata J,Umezawa A,Kiyokawa N

更新日期：2008-04-01 00:00:00

abstract：:We have constructed a genomic DNA library from a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400) in the cosmid vector pHC79. By utilizing a murine dihydrofolate reductase (DHFR) cDNA clone, we have identified 66 DHFR+ clones among the 11,000 colonies screened by colony hybridization. To isolate a rec...

journal_title：Molecular and cellular biology

authors： Milbrandt JD,Azizkhan JC,Greisen KS,Hamlin JL

更新日期：1983-07-01 00:00:00

abstract：:We have investigated the in vivo and in vitro regulation of the human urokinase-type plasminogen activator (uPA) gene by interleukin-1 (IL-1) and analyzed the transcription factors and signalling pathways involved in the response of the -2.0-kb uPA enhancer to IL-1 induction and to tetradecanoyl phorbol acetate (TPA) ...

journal_title：Molecular and cellular biology

authors： Cirillo G,Casalino L,Vallone D,Caracciolo A,De Cesare D,Verde P

更新日期：1999-09-01 00:00:00

abstract：:Interleukin 1 (IL-1) induces the synthesis of kappa immunoglobulin light chains and the expression of surface immunoglobulin in the murine pre-B-cell line 70Z/3 (J. G. Giri, P. W. Kincade, and S. B. Mizel, J. Immunol. 132:223-228, 1984). In the present study, we found that these effects of IL-1 are mimicked by cyclic ...

journal_title：Molecular and cellular biology

authors： Shirakawa F,Chedid M,Suttles J,Pollok BA,Mizel SB

更新日期：1989-03-01 00:00:00

abstract：:An interferon-alpha-like sequence was isolated from a human genomic library by hybridization with a 15-base oligonucleotide. The sequence also showed homology to alpha-interferon and was most closely related to the leukocyte interferon-M gene fragment. The original isolate cross-hybridized to a family of sequences, 10...

journal_title：Molecular and cellular biology

authors： Feinstein SI,Mory Y,Chernajovsky Y,Maroteaux L,Nir U,Lavie V,Revel M

更新日期：1985-03-01 00:00:00