Molecular mechanisms of COUP-TF-mediated transcriptional repression: evidence for transrepression and active repression.

abstract：:E6-AP is a 100-kDa cellular protein that mediates the interaction of the human papillomavirus type 16 and 18 E6 proteins with p53. The association of p53 with E6 and E6-AP promotes the specific ubiquitination and subsequent proteolytic degradation of p53 in vitro. We recently isolated a cDNA encoding E6-AP and have no...

journal_title：Molecular and cellular biology

authors： Huibregtse JM,Scheffner M,Howley PM

更新日期：1993-08-01 00:00:00

abstract：:In addition to the highly conserved AATAAA sequence, there is a requirement for specific sequences downstream of polyadenylic acid [poly(A)] cleavage sites to generate correct mRNA 3' termini. Previous experiments demonstrated that 35 nucleotides downstream of the E2A poly(A) site were sufficient but 20 nucleotides we...

journal_title：Molecular and cellular biology

authors： Hart RP,McDevitt MA,Ali H,Nevins JR

更新日期：1985-11-01 00:00:00

abstract：:The active beta-globin genes in chicken erythrocytes, like all active genes, reside in large chromatin domains which are preferentially sensitive to digestion by DNase I. We have recently proposed that the special structure of chromatin in active domains is maintained by torsional stress in the DNA (Villeponteau et al...

journal_title：Molecular and cellular biology

authors： Villeponteau B,Martinson HG

更新日期：1987-05-01 00:00:00

abstract：:Despite the identification of PBC proteins as cofactors that provide DNA affinity and binding specificity for the HOX homeodomain proteins, HOX proteins do not demonstrate robust activity in transient-transcription assays and few authentic downstream targets have been identified for these putative transcription factor...

journal_title：Molecular and cellular biology

authors： Shen WF,Krishnan K,Lawrence HJ,Largman C

更新日期：2001-11-01 00:00:00

abstract：:Tau, a major class of microtubule-associated proteins, consists of a family of proteins that are heterogeneous in molecular weight. The presence of internal deletions in previously described cDNA clones for murine and bovine tau suggested that alternative splicing of transcripts could account for the protein size hete...

journal_title：Molecular and cellular biology

authors： Himmler A

更新日期：1989-04-01 00:00:00

abstract：:Misregulation of NF-kappaB signaling leads to infectious, inflammatory, or autoimmune disorders. IkappaB kinase beta (IKKbeta) is an essential activator of NF-kappaB and is known to phosphorylate the NF-kappaB inhibitor, IkappaBalpha, allowing it to undergo ubiquitin-mediated proteasomal degradation. However, beyond I...

journal_title：Molecular and cellular biology

authors： Hutti JE,Turk BE,Asara JM,Ma A,Cantley LC,Abbott DW

更新日期：2007-11-01 00:00:00

abstract：:Yeast cells containing the recessive mas3 mutation display temperature-sensitive defects in both mitochondrial protein import and the cell division cycle. The import defect is characterized by two pools of mitochondrial precursors and a dramatically slower rate of posttranslational import. The effect of mas3 on cell c...

journal_title：Molecular and cellular biology

authors： Smith BJ,Yaffe MP

更新日期：1991-05-01 00:00:00

abstract：:Ligand-induced dimerization and transphosphorylation are thought to be important events by which receptor tyrosine kinases generate cellular signals. We have investigated the ability of signalling-defective, truncated fibroblast growth factor (FGF) receptors (FGFR-1 and FGFR-2) to block the FGF response in cells that ...

journal_title：Molecular and cellular biology

authors： Li Y,Basilico C,Mansukhani A

更新日期：1994-11-01 00:00:00

abstract：:How early- and late-firing origins are selected on eukaryotic chromosomes is largely unknown. Here, we show that Mrc1, a conserved factor required for stabilization of stalled replication forks, selectively binds to the early-firing origins in a manner independent of Cdc45 and Hsk1 kinase in the fission yeast Schizosa...

journal_title：Molecular and cellular biology

authors： Hayano M,Kanoh Y,Matsumoto S,Masai H

更新日期：2011-06-01 00:00:00

abstract：:Two cDNAs derived from Xenopus laevis calmodulin mRNA have been cloned. Both cDNAs contain the complete protein-coding region and various lengths of untranslated segments. The two cDNAs encode an identical protein but differ from each other by 5% nucleotide substitutions. The 5' and 3' untranslated regions, to the ext...

journal_title：Molecular and cellular biology

authors： Chien YH,Dawid IB

更新日期：1984-03-01 00:00:00

abstract：:Current amino acid and monosaccharide transport models are based on an assumption which equates the intracellular chemical activity of a solute with its concentration. This assumption was tested for alpha-aminoisobutyric acid and 3-O-methylglucose in a giant cell, the amphibian oocyte, by using recently developed cryo...

journal_title：Molecular and cellular biology

authors： Horowitz SB,Pearson TW

更新日期：1981-09-01 00:00:00

abstract：:PHAX (phosphorylated adaptor for RNA export) is the key regulator of U snRNA nuclear export in metazoa. Our previous work revealed that PHAX is phosphorylated in the nucleus and is exported as a component of the U snRNA export complex to the cytoplasm, where it is dephosphorylated (M. Ohno, A. Segref, A. Bachi, M. Wil...

journal_title：Molecular and cellular biology

authors： Kitao S,Segref A,Kast J,Wilm M,Mattaj IW,Ohno M

更新日期：2008-01-01 00:00:00

abstract：:The tumor suppressor p21 acts as a cell cycle inhibitor and has also been shown to regulate gene expression by functioning as a transcription corepressor. Here, we identified p21-regulated microRNAs (miRNAs) by sequencing small RNAs from isogenic p21(+/+) and p21(-/-) cells. Three abundant miRNA clusters, miR-200b-200...

journal_title：Molecular and cellular biology

authors： Li XL,Hara T,Choi Y,Subramanian M,Francis P,Bilke S,Walker RL,Pineda M,Zhu Y,Yang Y,Luo J,Wakefield LM,Brabletz T,Park BH,Sharma S,Chowdhury D,Meltzer PS,Lal A

更新日期：2014-02-01 00:00:00

abstract：:Spinal muscular atrophy (SMA) is an inherited motor neuron disease caused by homozygous loss of the Survival Motor Neuron 1 (SMN1) gene. In the absence of SMN1, inefficient inclusion of exon 7 in transcripts from the nearly identical SMN2 gene results in ubiquitous SMN decrease but selective motor neuron degeneration....

journal_title：Molecular and cellular biology

authors： Ruggiu M,McGovern VL,Lotti F,Saieva L,Li DK,Kariya S,Monani UR,Burghes AH,Pellizzoni L

更新日期：2012-01-01 00:00:00

abstract：:We report in vivo analysis of histone and RNA polymerase II (pol II) occupancy at the 601 element, which functions as a strong in vitro nucleosome-positioning element and transcriptional pause site. Surprisingly, nucleosomes were not strongly positioned over the 601 element inserted either within a yeast chromosomal o...

journal_title：Molecular and cellular biology

authors： Perales R,Zhang L,Bentley D

更新日期：2011-08-01 00:00:00

abstract：:Genetics and in vitro studies have shown that the direct interaction between Gal3p and Gal80p plays a central role in galactose-dependent Gal4p-mediated GAL gene expression in the yeast Saccharomyces cerevisiae. Precisely how Gal3p-Gal80p interaction effects induction is not clear. It has been assumed that Gal3p inter...

journal_title：Molecular and cellular biology

authors： Peng G,Hopper JE

更新日期：2000-07-01 00:00:00

abstract：:Inactivation of DNA mismatch repair by mutation or by transcriptional silencing of the MLH1 gene results in genome instability and cancer predisposition. We recently found (P. V. Shcherbakova and T. A. Kunkel, Mol. Cell. Biol. 19:3177-3183, 1999) that an elevated spontaneous mutation rate can also result from increase...

journal_title：Molecular and cellular biology

authors： Shcherbakova PV,Hall MC,Lewis MS,Bennett SE,Martin KJ,Bushel PR,Afshari CA,Kunkel TA

更新日期：2001-02-01 00:00:00

abstract：:Rad9 is required for the activation of DNA damage checkpoint pathways in budding yeast. Rad9 is phosphorylated after DNA damage in a Mec1- and Tel1-dependent manner and subsequently interacts with Rad53. This Rad9-Rad53 interaction has been suggested to trigger the activation and phosphorylation of Rad53. Here we show...

journal_title：Molecular and cellular biology

authors： Naiki T,Wakayama T,Nakada D,Matsumoto K,Sugimoto K

更新日期：2004-04-01 00:00:00

abstract：:The mammalian signal recognition particle (SRP) is a small cytoplasmic ribonucleoprotein required for the cotranslational targeting of secretory proteins to the endoplasmic reticulum membrane. The heterodimeric protein subunit SRP9/14 was previously shown to be essential for SRP to cause pausing in the elongation of s...

journal_title：Molecular and cellular biology

authors： Strub K,Moss J,Walter P

更新日期：1991-08-01 00:00:00

abstract：:RNA editing of specific residues by adenosine deamination is a nuclear process catalyzed by adenosine deaminases acting on RNA (ADAR). Different promoters in the ADAR1 gene give rise to two forms of the protein: a constitutive promoter expresses a transcript encoding (c)ADAR1, and an interferon-induced promoter expres...

journal_title：Molecular and cellular biology

authors： Poulsen H,Nilsson J,Damgaard CK,Egebjerg J,Kjems J

更新日期：2001-11-01 00:00:00

abstract：:Quantitative immunoelectron microscopy and subcellular fractionation established the site of endoplasmic reticulum (ER)-Golgi transport arrest induced by the phosphatase inhibitor okadaic acid (OA). OA induced the disappearance of transitional element tubules and accumulation of the anterograde-transported Chandipura ...

journal_title：Molecular and cellular biology

authors： Pryde JG,Farmaki T,Lucocq JM

更新日期：1998-02-01 00:00:00

abstract：:Mutations in the spin gene are characterized by an extraordinarily strong rejection behavior of female flies in response to male courtship. They are also accompanied by decreases in the viability, adult life span, and oviposition rate of the flies. In spin mutants, some oocytes and adult neural cells undergo degenerat...

journal_title：Molecular and cellular biology

authors： Nakano Y,Fujitani K,Kurihara J,Ragan J,Usui-Aoki K,Shimoda L,Lukacsovich T,Suzuki K,Sezaki M,Sano Y,Ueda R,Awano W,Kaneda M,Umeda M,Yamamoto D

更新日期：2001-06-01 00:00:00

abstract：:Poly(A) signals located at the 3' end of eukaryotic genes drive cleavage and polyadenylation at the same end of pre-mRNA. Although these sequences are expected only at the 3' end of genes, we found that strong poly(A) signals are also predicted within the 5' untranslated regions (UTRs) of many Drosophila melanogaster ...

journal_title：Molecular and cellular biology

authors： Guo J,Garrett M,Micklem G,Brogna S

更新日期：2011-02-01 00:00:00

abstract：:The protein kinase Akt plays a critical role in heart function and is activated by phosphorylation of threonine 308 (T308) and serine 473 (S473). While phosphoinositide-dependent kinase 1 (PDK1) is responsible for Akt T308 phosphorylation, the identities of the kinases for Akt S473 phosphorylation in the heart remain ...

journal_title：Molecular and cellular biology

authors： Zhao X,Lu S,Nie J,Hu X,Luo W,Wu X,Liu H,Feng Q,Chang Z,Liu Y,Cao Y,Sun H,Li X,Hu Y,Yang Z

更新日期：2014-06-01 00:00:00

abstract：:Sequence blocks within the core region were swapped among RNA polymerase II promoters to explore effects on transcription in vitro. The pair of blocks flanking TATA strongly influenced general transcription, with an additional effect on promoter activation. These flanking elements induced a change in the ratio of acti...

journal_title：Molecular and cellular biology

authors： Wolner BS,Gralla JD

更新日期：2000-05-01 00:00:00

abstract：:In Drosophila melanogaster, achaete (ac) and m8 are model basic helix-loop-helix activator (bHLH A) and repressor genes, respectively, that have the opposite cell expression pattern in proneural clusters during Notch signaling. Previous studies have shown that activation of m8 transcription in specific cells within pr...

journal_title：Molecular and cellular biology

authors： Cave JW,Xia L,Caudy M

更新日期：2011-01-01 00:00:00

abstract：:Chronic myelogenous leukemia (CML) is a clonal myeloproliferative disorder resulting from the neoplastic transformation of a hematopoietic stem cell. The majority of cases of CML are associated with the (9;22) chromosome translocation that generates the bcr-abl chimeric gene. Alpha interferon (IFN-alpha) treatment ind...

journal_title：Molecular and cellular biology

authors： Hao SX,Ren R

更新日期：2000-02-01 00:00:00

abstract：:Bach2 is a B-cell- and neuron-specific transcription repressor that forms heterodimers with the Maf-related oncoproteins. We show here that Bach2 activates transcription by interacting with its novel partner MAZR. MAZR was isolated by the yeast two-hybrid screen using the BTB/POZ domain of Bach2 as bait. Besides the B...

journal_title：Molecular and cellular biology

authors： Kobayashi A,Yamagiwa H,Hoshino H,Muto A,Sato K,Morita M,Hayashi N,Yamamoto M,Igarashi K

更新日期：2000-03-01 00:00:00

abstract：:The E2F1, -2, and -3 transcription factors are key downstream targets of the retinoblastoma protein (pRB) tumor suppressor that drive expression of proliferation-associated genes. Here we use mutant mouse strains to investigate E2F3's role in vivo. We show that E2F3 is essential for embryonic viability in the pure 129...

journal_title：Molecular and cellular biology

authors： Cloud JE,Rogers C,Reza TL,Ziebold U,Stone JR,Picard MH,Caron AM,Bronson RT,Lees JA

更新日期：2002-04-01 00:00:00

abstract：:A cDNA encoding a novel Krüppel-type zinc finger protein, FKLF, was cloned from fetal globin-expressing human fetal erythroid cells. The deduced polypeptide sequence composed of 512 amino acids revealed that, like Sp1 and EKLF, FKLF has three contiguous zinc fingers at the near carboxyl-terminal end. A long amino-term...

journal_title：Molecular and cellular biology

authors： Asano H,Li XS,Stamatoyannopoulos G

更新日期：1999-05-01 00:00:00