Abstract:
:Sequence blocks within the core region were swapped among RNA polymerase II promoters to explore effects on transcription in vitro. The pair of blocks flanking TATA strongly influenced general transcription, with an additional effect on promoter activation. These flanking elements induced a change in the ratio of activated to basal transcription, whereas swapping TATA and initiator sequences only altered general transcription levels. Swapping the flanking blocks influenced binding by general transcription factors TBP and TFIIB. The results suggest that the architecture of the extended core sequence is important in determining promoter-specific effects on both general transcription levels and the tightness of regulation.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Wolner BS,Gralla JDdoi
10.1128/mcb.20.10.3608-3615.2000subject
Has Abstractpub_date
2000-05-01 00:00:00pages
3608-15issue
10eissn
0270-7306issn
1098-5549journal_volume
20pub_type
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