Abstract:
:Recognition of gene promoters by RNA polymerase II is mediated by general transcription factor IID (TFIID), which has been thought to be a static complex and to play a passive role in the regulation of gene expression under the instruction of gene-specific transcription factors. Here we show that transforming growth factor β (TGF-β) induced degradation of the TFIID subunit TAF7 in cultured mouse mammary epithelial cells and that this effect was required for proliferative arrest in response to TGF-β stimulation. TGF-β stimulated transcription of the gene for the ubiquitin ligase TRIM26, which was shown to ubiquitylate TAF7 and thereby to target it for proteasomal degradation. Sustained exposure of cells to TGF-β resulted in recovery from proliferative arrest in association with amplification of the Myc proto-oncogene, with MYC inhibiting TRIM26 induction by TGF-β. Our data thus show that TFIID is not simply a general mediator of transcription but contributes to the regulation of transcription in response to cell stimulation, playing a key role in the cytostatic function of TGF-β.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Nakagawa T,Hosogane M,Nakagawa M,Morohoshi A,Funayama R,Nakayama Kdoi
10.1128/MCB.00449-17subject
Has Abstractpub_date
2018-02-12 00:00:00issue
5eissn
0270-7306issn
1098-5549pii
MCB.00449-17journal_volume
38pub_type
杂志文章abstract::Acquisition of the ability to produce gamma interferon (IFN-gamma) is a fundamental property of memory T cells and enables one subset (T helper 1 [TH1]) to deliver its effector functions. To examine regulation of IFN-gamma gene expression in a model system which recapitulates TH1 differentiation, we prepared reporter ...
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pub_type: 杂志文章
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pub_type: 杂志文章
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