Targeting SQSTM1/p62 induces cargo loading failure and converts autophagy to apoptosis via NBK/Bik.

abstract：:Multiple organ systems require epithelial barriers for normal function, and barrier loss is a hallmark of diseases ranging from inflammation to epithelial cancers. However, the molecular processes regulating epithelial barrier maturation are not fully elucidated. After contact, epithelial cells undergo size-reductive ...

journal_title：Molecular and cellular biology

authors： Bolinger MT,Ramshekar A,Waldschmidt HV,Larsen SD,Bewley MC,Flanagan JM,Antonetti DA

更新日期：2016-07-14 00:00:00

abstract：:Yju2 is an essential splicing factor required for the first catalytic step after the action of Prp2. We dissected the structure of Yju2 and found that the amino (Yju2-N) and carboxyl (Yju2-C) halves of the protein can be separated and reconstituted for Yju2 function both in vivo and in vitro. Yju2-N has a weak affinit...

journal_title：Molecular and cellular biology

authors： Chiang TW,Cheng SC

更新日期：2013-05-01 00:00:00

abstract：:Activation of canonical Wnt signaling inhibits brown adipogenesis of cultured cells by impeding induction of PPARgamma and C/EBPalpha. Although enforced expression of these adipogenic transcription factors restores lipid accumulation and expression of FABP4 in Wnt-expressing cells, additional expression of PGC-1alpha ...

journal_title：Molecular and cellular biology

authors： Kang S,Bajnok L,Longo KA,Petersen RK,Hansen JB,Kristiansen K,MacDougald OA

更新日期：2005-02-01 00:00:00

abstract：:Swi4 and Swi6 form a complex which is required for Start-dependent activation of HO and for high-level expression of G1 cyclin genes CLN1 and CLN2. To identify other regulators of this pathway, we screened for dominant, recessive, conditional, and allele-specific suppressors of swi4 mutants. We isolated 16 recessive s...

journal_title：Molecular and cellular biology

authors： Lycan D,Mikesell G,Bunger M,Breeden L

更新日期：1994-11-01 00:00:00

abstract：:The Id subfamily of helix-loop-helix (HLH) proteins plays a fundamental role in the regulation of cellular proliferation and differentiation. The major mechanism by which Id proteins are thought to inhibit differentiation is through interaction with other HLH proteins and inhibition of their DNA-binding activity. Howe...

journal_title：Molecular and cellular biology

authors： Roberts EC,Deed RW,Inoue T,Norton JD,Sharrocks AD

更新日期：2001-01-01 00:00:00

abstract：:Growth, patterning, and apoptosis are mutually interactive during development. For example, cells that select an abnormal fate in a developing field are frequently removed by apoptosis. An important issue in this process that needs to be resolved is the mechanism used by cells to discern their correct fate from an abn...

journal_title：Molecular and cellular biology

authors： Adachi-Yamada T,Harumoto T,Sakurai K,Ueda R,Saigo K,O'Connor MB,Nakato H

更新日期：2005-04-01 00:00:00

abstract：:Transfection of C3H/10T1/2 cells with a c-myc gene resulted in the acquisition of responsiveness to transforming growth factor type beta. Cells transfected with an activated H-ras gene or an H-ras and c-myc gene, however, exhibited a transformed morphology and spontaneous soft-agar growth, a phenotype induced reversib...

journal_title：Molecular and cellular biology

authors： Leof EB,Proper JA,Moses HL

更新日期：1987-07-01 00:00:00

abstract：:The cellular response to DNA damage is an intricate mechanism that involves the interplay among several pathways. In this study, we provide evidence of the roles of the polyadenylation factor cleavage stimulation factor 50 (CstF-50) and the ubiquitin (Ub) escort factor p97 as cofactors of BRCA1/BARD1 E3 Ub ligase, fac...

journal_title：Molecular and cellular biology

authors： Fonseca D,Baquero J,Murphy MR,Aruggoda G,Varriano S,Sapienza C,Mashadova O,Rahman S,Kleiman FE

更新日期：2018-01-29 00:00:00

abstract：:The nuclear receptor peroxisome proliferator-activated receptor gamma regulates adipose differentiation and systemic insulin signaling via ligand-dependent transcriptional activation of target genes. However, the identities of the biologically relevant target genes are largely unknown. Here we describe the isolation a...

journal_title：Molecular and cellular biology

authors： Yoon JC,Chickering TW,Rosen ED,Dussault B,Qin Y,Soukas A,Friedman JM,Holmes WE,Spiegelman BM

更新日期：2000-07-01 00:00:00

abstract：:A cDNA library enriched with Myc-responsive cDNAs but depleted of myc cDNAs was used in a functional screen for growth enhancement in c-myc-null cells. A cDNA clone for mitochondrial serine hydroxymethyltransferase (mSHMT) that was capable of partial complementation of the growth defects of c-myc-null cells was identi...

journal_title：Molecular and cellular biology

authors： Nikiforov MA,Chandriani S,O'Connell B,Petrenko O,Kotenko I,Beavis A,Sedivy JM,Cole MD

更新日期：2002-08-01 00:00:00

abstract：:Cotranslational insertion of type I collagen chains into the lumen of the endoplasmic reticulum (ER) and their subsequent folding into a heterotrimeric helix is a complex process which requires coordinated action of the translation machinery, components of translocons, molecular chaperones, and modifying enzymes. Here...

journal_title：Molecular and cellular biology

authors： Stefanovic B,Stefanovic L,Schnabl B,Bataller R,Brenner DA

更新日期：2004-02-01 00:00:00

abstract：:Hepatocyte nuclear factor 4 (HNF-4), a highly conserved member of the steroid hormone receptor superfamily critical for development and liver-specific gene expression, is very similar to another superfamily member, retinoid X receptor alpha (RXR alpha), in overall amino acid sequence and DNA binding specificity. Since...

journal_title：Molecular and cellular biology

authors： Jiang G,Nepomuceno L,Hopkins K,Sladek FM

更新日期：1995-09-01 00:00:00

abstract：:Inactivation of the tumor suppressor p53 by degradation is a mechanism utilized by cells to adapt to endoplasmic reticulum (ER) stress. However, the mechanisms of p53 destabilization by ER stress are not known. We demonstrate here that the E3 ubiquitin-ligase Hdm2 is essential for the nucleocytoplasmic transport and p...

journal_title：Molecular and cellular biology

authors： Pluquet O,Qu LK,Baltzis D,Koromilas AE

更新日期：2005-11-01 00:00:00

abstract：:Cell lines established from the lepidopteran insect Spodoptera frugiperda (fall armyworm; Sf9) are used routinely as hosts for the expression of foreign proteins by recombinant baculovirus vectors. We have examined the pathway of protein glycosylation and secretion in these cells, using human tissue plasminogen activa...

journal_title：Molecular and cellular biology

authors： Jarvis DL,Summers MD

更新日期：1989-01-01 00:00:00

abstract：:Transfection of a functional cloned p53 gene into an L12 p53 nonproducer cell line efficiently reconstituted p53 expression. The p53 protein synthesized in these clones was indistinguishable from that occurring naturally in tumor cells. When a p53 cDNA clone was used instead, we observed that the L12-derived clones ex...

journal_title：Molecular and cellular biology

authors： Arai N,Nomura D,Yokota K,Wolf D,Brill E,Shohat O,Rotter V

更新日期：1986-09-01 00:00:00

abstract：:The signaling routes linking G-protein-coupled receptors to mitogen-activated protein kinase (MAPK) may involve tyrosine kinases, phosphoinositide 3-kinase gamma (PI3Kgamma), and protein kinase C (PKC). To characterize the mitogenic pathway of bradykinin (BK), COS-7 cells were transiently cotransfected with the human ...

journal_title：Molecular and cellular biology

authors： Adomeit A,Graness A,Gross S,Seedorf K,Wetzker R,Liebmann C

更新日期：1999-08-01 00:00:00

abstract：:The human vitamin D-binding protein (hDBP) gene exists in a cluster of four liver-expressed genes. A minimal hDBP transgene, containing a defined set of liver-specific DNase I hypersensitive sites (HSs), is robustly expressed in mouse liver in a copy-number-dependent manner. Here we evaluate these HSs for function. De...

journal_title：Molecular and cellular biology

authors： Hiroki T,Liebhaber SA,Cooke NE

更新日期：2007-11-01 00:00:00

abstract：:The Ran binding protein RanBP1 is localized to the cytosol of interphase cells. A leucine-rich nuclear export signal (NES) near the C terminus of RanBP1 is essential to maintain this distribution. We now show that RanBP1 accumulates in nuclei of cells treated with the export inhibitor, leptomycin B, and collapse of th...

journal_title：Molecular and cellular biology

authors： Plafker K,Macara IG

更新日期：2000-05-01 00:00:00

abstract：:A truncated H-2Ld gene was constructed by deleting the transmembrane and cytoplasmic exons. The truncated H-2Ld gene was introduced into mouse L cells using the thymidine kinase gene as a selectable marker. Transformants were isolated and screened for the presence of truncated H-2Ld antigen. The truncated H-2Ld gene p...

journal_title：Molecular and cellular biology

authors： Murre C,Parker KC,Reiss CS,Driller JP,Wiley DC,Burakoff SJ,Seidman JG

更新日期：1986-04-01 00:00:00

abstract：:Physiological cell deaths occur ubiquitously throughout biology and have common attributes, including apoptotic morphology with mitosis-like chromatin condensation and prelytic genome digestion. The fundamental question is whether a common mechanism of dying underlies these common hallmarks of death. Here we describe ...

journal_title：Molecular and cellular biology

authors： Harvey KJ,Blomquist JF,Ucker DS

更新日期：1998-05-01 00:00:00

abstract：:We have functionally tested derivatives of a muscle-specific Drosophila melanogaster actin gene in which 5'-flanking sequences have been deleted or rearranged. From our results we conclude that approximately 1,000 nucleotides of 5'-flanking sequence are required for wild-type levels of mRNA accumulation during flight ...

journal_title：Molecular and cellular biology

authors： Geyer PK,Fyrberg EA

更新日期：1986-10-01 00:00:00

abstract：:Ligand activation of Notch receptors leads to release of the intracellular receptor domain (Notch IC), which translocates to the nucleus and interacts with the DNA-binding protein RBP-Jkappa to control expression of specific target genes. A number of proteins have been shown to interact with Notch ICs and to modulate ...

journal_title：Molecular and cellular biology

authors： Wallberg AE,Pedersen K,Lendahl U,Roeder RG

更新日期：2002-11-01 00:00:00

abstract：:RNA polymerase III (pol III) transcripts of the highly repeated mouse B2 gene family are increased in many oncogenically transformed murine cell lines. In cells transformed by simian virus 40, the small, cytoplasmic B2 RNAs are present at 20-fold-higher levels than in normal cells (M. R. D. Scott, K. Westphal, and P. ...

journal_title：Molecular and cellular biology

authors： Carey MF,Singh K,Botchan M,Cozzarelli NR

更新日期：1986-09-01 00:00:00

abstract：:Fragments of African green monkey (Cercopithecus aethiops) DNA (3.5 to 18.0 kilobases) were inserted downstream from the thymidine kinase (TK, tk) coding region in pTK206/SV010, a gene construct which lacks both copies of the hexanucleotide 5'-AATAAA-3' and contains a simian virus 40 origin of replication, allowing it...

journal_title：Molecular and cellular biology

authors： Santangelo GM,Cole CN

更新日期：1983-04-01 00:00:00

abstract：:Ribosomal (r) proteins encoded by polyadenylated RNA were specifically precipitated in vitro from polysomes by using antibodies raised against characterized Drosophila melanogaster r proteins. The immuno-purified mRNA in the polysome complex was used to prepare cDNA with which to probe a D. melanogaster genomic librar...

journal_title：Molecular and cellular biology

authors： Burns DK,Stark BC,Macklin MD,Chooi WY

更新日期：1984-12-01 00:00:00

abstract：:A late region deletion mutant of simian virus 40 (dl5) was previously shown to be deficient in the transport of nuclear RNA. This is a splice junction deletion that has lost the 3' end of an RNA leader, an intervening sequence, and the 5' end of the splice acceptor site on the body of the mRNA. In this report, we anal...

journal_title：Molecular and cellular biology

authors： Villarreal LP,White RT

更新日期：1983-08-01 00:00:00

abstract：:In Saccharomyces cerevisiae, 3-amino-1,2,4-triazole (aminotriazole) competitively inhibits the activity of imidazoleglycerolphosphate dehydratase, the product of the HIS3 gene. Wild-type strains are able to grow in the presence of 10 mM aminotriazole because they induce the level of imidazoleglycerolphosphate dehydrat...

journal_title：Molecular and cellular biology

authors： Kanazawa S,Driscoll M,Struhl K

更新日期：1988-02-01 00:00:00

abstract：:Regulated transcription of class II genes of the yeast Saccharomyces cerevisiae requires the diverse functions of mediator complex. In particular, MED6 is essential for activated transcription from many class II promoters, suggesting that it functions as a key player in the relay of activator signals to the basal tran...

journal_title：Molecular and cellular biology

authors： Lee YC,Kim YJ

更新日期：1998-09-01 00:00:00

abstract：:The activity of p21ras is required for the proliferative response to colony-stimulating factor 1 (CSF-1), and signals transduced by both the CSF-1 receptor (CSF-1R) and p21ras stimulate transcription from promoter elements containing overlapping binding sites for Fos/Jun- and Ets-related proteins. A sequence encoding ...

journal_title：Molecular and cellular biology

authors： Langer SJ,Bortner DM,Roussel MF,Sherr CJ,Ostrowski MC

更新日期：1992-12-01 00:00:00

abstract：:The E1a gene of adenovirus encodes two proteins, 289 and 243 amino acids long, which have positive (transactivator) and negative (enhancer repressor) RNA polymerase II transcriptional regulatory properties and cell transformation activities including cooperation with an activated ras gene. The E1a transforming functio...

journal_title：Molecular and cellular biology

authors： Velcich A,Ziff E

更新日期：1988-05-01 00:00:00