Abstract:
:A truncated H-2Ld gene was constructed by deleting the transmembrane and cytoplasmic exons. The truncated H-2Ld gene was introduced into mouse L cells using the thymidine kinase gene as a selectable marker. Transformants were isolated and screened for the presence of truncated H-2Ld antigen. The truncated H-2Ld gene product was present in both the cytoplasm and culture medium, but not on the cell surface. The truncated H-2Ld antigen was stable in culture medium for at least 9 h and was secreted into the medium at a rate similar to the kinetics with which complete H-2 antigens reach the cell surface. Transformants expressing the truncated H-2Ld molecule were not recognized by cytotoxic T lymphocytes specific for the H-2Ld antigen.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Murre C,Parker KC,Reiss CS,Driller JP,Wiley DC,Burakoff SJ,Seidman JGdoi
10.1128/mcb.6.4.1315subject
Has Abstractpub_date
1986-04-01 00:00:00pages
1315-9issue
4eissn
0270-7306issn
1098-5549journal_volume
6pub_type
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