Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins.

abstract：:The effect of atenolol, propranolol, trifluoperazine, and caffeine on the occurrence of meiotic diploid and disomic products in Saccharomyces cerevisiae was investigated. We demonstrated that atenolol, propranolol, and trifluoperazine reduce the occurrence of meiotic diploid products and that propranolol also slightly...

journal_title：Molecular and cellular biology

authors： Sora S,Bianchi M

更新日期：1982-11-01 00:00:00

abstract：:Human neuroblastoma cells often carry amplified DNA encompassing the gene N-myc. Amplified N-myc has been found localized in "double minutes" in direct tumor cell preparations. In contrast, later passages carried amplified N-myc almost exclusively within a single homogeneously staining chromosomal region located at a ...

journal_title：Molecular and cellular biology

authors： Amler LC,Schwab M

更新日期：1989-11-01 00:00:00

abstract：:We have examined the possible role of adenosine 3',5'-phosphate (cAMP) in functions associated with the plasma membranes of Saccharomyces cerevisiae. Purified membranes from this source contained an adenylate cyclase which was insensitive to activation by fluoride or guanine nucleotides, only weakly responsive to chan...

journal_title：Molecular and cellular biology

authors： Jaynes PK,McDonough JP,Mahler HR

更新日期：1982-12-01 00:00:00

abstract：:All inbred strains of mice carry the Ren-1 structural gene, which encodes the renin-1 isozyme, the classical renin activity found in kidneys. In addition, some strains carry a second renin structural gene, Ren-2, which encodes the predominantly expressed submaxillary gland renin isozyme, renin-2. Ren-1 and Ren-2 exhib...

journal_title：Molecular and cellular biology

authors： Field LJ,Philbrick WM,Howles PN,Dickinson DP,McGowan RA,Gross KW

更新日期：1984-11-01 00:00:00

abstract：:Generally, histone deacetylase (HDAC) inhibitor-induced p21(Waf1/Cip1) expression is thought to be p53 independent. Here we found that an inhibitor of HDAC, depsipeptide (FR901228), but not trichostatin A (TSA), induces p21(Waf1/Cip1) expression through both p53 and Sp1/Sp3 pathways in A549 cells (which retain wild-ty...

journal_title：Molecular and cellular biology

authors： Zhao Y,Lu S,Wu L,Chai G,Wang H,Chen Y,Sun J,Yu Y,Zhou W,Zheng Q,Wu M,Otterson GA,Zhu WG

更新日期：2006-04-01 00:00:00

abstract：:Miz1 is a member of the POZ domain/zinc finger transcription factor family. In vivo, Miz1 forms a complex with the Myc oncoprotein and recruits Myc to core promoter elements. Myc represses transcription through Miz1 binding sites. We now show that the Miz1 gene is ubiquitously expressed during mouse embryogenesis. In ...

journal_title：Molecular and cellular biology

authors： Adhikary S,Peukert K,Karsunky H,Beuger V,Lutz W,Elsässer HP,Möröy T,Eilers M

更新日期：2003-11-01 00:00:00

abstract：:Proteins encoded by the mdm2 gene, which has a pivotal role in the regulation of growth and differentiation, exist principally in human and murine cells as two isoforms that migrate in gels as 75-kDa and 90-kDa proteins. There is limited understanding of the respective biological roles of these isoforms, their molecul...

journal_title：Molecular and cellular biology

authors： Cheng TH,Cohen SN

更新日期：2007-01-01 00:00:00

abstract：:While the normal human erbB-2 gene is potently transforming when overexpressed in NIH 3T3 cells, its rat homolog, the neu gene, seems to acquire transforming properties only upon alteration of its coding sequence. In this study, we compared the effects of different levels of expression of normal erbB-2 and neu in NIH ...

journal_title：Molecular and cellular biology

authors： Di Marco E,Pierce JH,Knicley CL,Di Fiore PP

更新日期：1990-06-01 00:00:00

abstract：:Cyclin B interacts with Cdc2 kinase to induce cell cycle events, particularly those of mitosis. The existence of cyclin B subtypes in several species has been known for some time, leading to speculation that key events of mitosis may be carried out by distinct functional classes of Cdc2/cyclin B. We report the discove...

journal_title：Molecular and cellular biology

authors： Bueno A,Russell P

更新日期：1993-04-01 00:00:00

abstract：:The mouse urokinase-type plasminogen activator (uPA) gene was used as a model macrophage colony-stimulating factor 1 (CSF-1)-inducible gene to investigate CSF-1 signalling pathways. Nuclear run-on analysis showed that induction of uPA mRNA by CSF-1 and phorbol myristate acetate (PMA) was at the transcriptional level i...

journal_title：Molecular and cellular biology

authors： Stacey KJ,Fowles LF,Colman MS,Ostrowski MC,Hume DA

更新日期：1995-06-01 00:00:00

abstract：:Liu and Mertz (Genes Dev. 9:1766-1780, 1995) previously identified a 119-nt pre-mRNA processing enhancer (PPE) element within the herpes simplex virus type 1 thymidine kinase gene that enables intron-independent gene expression in higher eukaryotes by binding heterogeneous nuclear ribonucleoprotein L (hnRNP L). Here, ...

journal_title：Molecular and cellular biology

authors： Guang S,Felthauser AM,Mertz JE

更新日期：2005-08-01 00:00:00

abstract：:The gene responsible for multidrug resistance (mdr), which encodes the P-glycoprotein, is a member of a multigene family. We have identified distinct mdr gene transcripts encoded by three separate mdr genes in the mouse. Expression levels of each mdr gene are dramatically different in various mouse tissues. Specific m...

journal_title：Molecular and cellular biology

authors： Croop JM,Raymond M,Haber D,Devault A,Arceci RJ,Gros P,Housman DE

更新日期：1989-03-01 00:00:00

abstract：:It has previously been demonstrated that the simian virus 40 large T antigen and adenovirus E1A proteins can form complexes with the retinoblastoma susceptibility gene product (RB). We studied the ability of these proteins to bind to mutant RB proteins in vitro. A region of RB spanning residues 379 to 792 was found to...

journal_title：Molecular and cellular biology

authors： Kaelin WG Jr,Ewen ME,Livingston DM

更新日期：1990-07-01 00:00:00

abstract：:Protein ubiquitination plays a key role in the regulation of a variety of DNA repair mechanisms. Protein ubiquitination is controlled by the coordinate activity of ubiquitin ligases and deubiquitinating enzymes (DUBs). The deubiquitinating enzyme USP1 regulates DNA repair and the Fanconi anemia pathway through its ass...

journal_title：Molecular and cellular biology

authors： Murai J,Yang K,Dejsuphong D,Hirota K,Takeda S,D'Andrea AD

更新日期：2011-06-01 00:00:00

abstract：:The c-myc proto-oncogene encodes a transcription factor that participates in the regulation of cellular proliferation, differentiation, and apoptosis. Ectopic overexpression of c-Myc has been shown to sensitize cells to apoptosis. We report here that cells lacking c-Myc activity due to disruption of the c-myc gene by ...

journal_title：Molecular and cellular biology

authors： Adachi S,Obaya AJ,Han Z,Ramos-Desimone N,Wyche JH,Sedivy JM

更新日期：2001-08-01 00:00:00

abstract：:The establishment of epigenetic marks, such as methylation on histone tails, is mechanistically linked to RNA polymerase II within active genes. To explore the interplay between these modifications in transcribed noncoding as well as coding sequences, we analyzed epigenetic modification and chromatin structure at high...

journal_title：Molecular and cellular biology

authors： Kim A,Kiefer CM,Dean A

更新日期：2007-02-01 00:00:00

abstract：:Telomerase is a ribonucleoprotein reverse transcriptase responsible for the maintenance of one strand of the telomere terminal repeats. The catalytic protein subunit of the telomerase complex, known as TERT, possesses a reverse transcriptase (RT) domain that mediates nucleotide addition. The RT domain of TERT is disti...

journal_title：Molecular and cellular biology

authors： Lue NF,Lin YC,Mian IS

更新日期：2003-12-01 00:00:00

abstract：:Using a hybridoma cell line which secretes hapten-specific immunoglobulin M (IgM), we have isolated a variety of mutants which produce abnormal immunoglobulin. Immunoglobulin was tested for the size and composition of the component heavy and light chains and for variable and constant region related functional and sero...

journal_title：Molecular and cellular biology

authors： Shulman MJ,Heusser C,Filkin C,Köhler G

更新日期：1982-09-01 00:00:00

abstract：:As part of an effort to characterize the spatial and functional relationships among genetic elements within the amplified dihydrofolate reductase (DHFR) domain in Chinese hamster cells, we have used a variation of the differential hybridization approach to identify cDNA clones whose genes are coamplified with DHFR in ...

journal_title：Molecular and cellular biology

authors： Foreman PK,Hamlin JL

更新日期：1989-03-01 00:00:00

abstract：:The translocation (6;9)(p23;q34) in acute nonlymphocytic leukemia results in the formation of a highly consistent dek-can fusion gene. Translocation breakpoints invariably occur in single introns of dek and can, which were named icb-6 and icb-9, respectively. In a case of acute undifferentiated leukemia, a breakpoint ...

journal_title：Molecular and cellular biology

authors： von Lindern M,van Baal S,Wiegant J,Raap A,Hagemeijer A,Grosveld G

更新日期：1992-08-01 00:00:00

abstract：:Trabecular myocardium accounts for the majority of the ventricles during early cardiogenesis, but compact myocardium is the primary component at later developmental stages. Elucidation of the genes regulating compact myocardium development is essential to increase our understanding of left ventricular noncompaction (L...

journal_title：Molecular and cellular biology

authors： Kosaka Y,Cieslik KA,Li L,Lezin G,Maguire CT,Saijoh Y,Toyo-oka K,Gambello MJ,Vatta M,Wynshaw-Boris A,Baldini A,Yost HJ,Brunelli L

更新日期：2012-12-01 00:00:00

abstract：:Transgenic mice containing a sheep metallothionein 1a-sheep growth hormone fusion gene exhibited low, tissue-specific basal levels of transgene mRNA expression, resulting in slightly elevated levels of circulating growth hormone that did not lead to a detectable increase in growth. After zinc stimulation, high levels ...

journal_title：Molecular and cellular biology

authors： Shanahan CM,Rigby NW,Murray JD,Marshall JT,Townrow CA,Nancarrow CD,Ward KA

更新日期：1989-12-01 00:00:00

abstract：:By using a model system for cell transformation mediated by the cooperation of the activated H-ras oncogene and the inactivated p53 tumor suppressor gene, rCop-1 was identified by mRNA differential display as a gene whose expression became lost after cell transformation. Homology analysis indicates that rCop-1 belongs...

journal_title：Molecular and cellular biology

authors： Zhang R,Averboukh L,Zhu W,Zhang H,Jo H,Dempsey PJ,Coffey RJ,Pardee AB,Liang P

更新日期：1998-10-01 00:00:00

abstract：:Transposons have contributed protein coding sequences to a unexpectedly large number of human genes. Except for the V(D)J recombinase and telomerase, all remain of unknown function. Here we investigate the activity of the human SETMAR protein, a highly expressed fusion between a histone H3 methylase and a mariner fami...

journal_title：Molecular and cellular biology

authors： Liu D,Bischerour J,Siddique A,Buisine N,Bigot Y,Chalmers R

更新日期：2007-02-01 00:00:00

abstract：:To investigate the role of insulin receptor substrate 1 (IRS-1) and IRS-2, the two ubiquitously expressed IRS proteins, in adipocyte differentiation, we established embryonic fibroblast cells with four different genotypes, i.e., wild-type, IRS-1 deficient (IRS-1(-/-)), IRS-2 deficient (IRS-2(-/-)), and IRS-1 IRS-2 dou...

journal_title：Molecular and cellular biology

authors： Miki H,Yamauchi T,Suzuki R,Komeda K,Tsuchida A,Kubota N,Terauchi Y,Kamon J,Kaburagi Y,Matsui J,Akanuma Y,Nagai R,Kimura S,Tobe K,Kadowaki T

更新日期：2001-04-01 00:00:00

abstract：:Entry into mitosis is controlled by the cyclin-dependent kinase CDK1 and can be delayed in response to DNA damage. In some systems, such G(2)/M arrest has been shown to reflect the stabilization of inhibitory phosphorylation sites on CDK1. In human cells, full G(2) arrest appears to involve additional mechanisms. We d...

journal_title：Molecular and cellular biology

authors： Badie C,Itzhaki JE,Sullivan MJ,Carpenter AJ,Porter AC

更新日期：2000-04-01 00:00:00

abstract：:The accumulation of assembled holoenzymes composed of regulatory D-type cyclins and their catalytic partner, cyclin-dependent kinase 4 (cdk4), is rate limiting for progression through the G1 phase of the cell cycle in mammalian fibroblasts. Both the synthesis and assembly of D-type cyclins and cdk4 depend upon serum s...

journal_title：Molecular and cellular biology

authors： Kato JY,Matsuoka M,Strom DK,Sherr CJ

更新日期：1994-04-01 00:00:00

abstract：:The silent mating-type loci of Saccharomyces cerevisiae, HML and HMR, are flanked by transcriptional silencers that have ARS activity (i.e., they function as replication origins when in plasmids). To test whether these ARS elements are chromosomal origins, we mapped origins near HML (close to the left telomere of chro...

journal_title：Molecular and cellular biology

authors： Dubey DD,Davis LR,Greenfeder SA,Ong LY,Zhu JG,Broach JR,Newlon CS,Huberman JA

更新日期：1991-10-01 00:00:00

abstract：:Cancer cells often heavily depend on the ubiquitin-proteasome system (UPS) for their growth and survival. Irrespective of their strong dependence on the proteasome activity, cancer cells, except for multiple myeloma, are mostly resistant to proteasome inhibitors. A major cause of this resistance is the proteasome boun...

journal_title：Molecular and cellular biology

authors： Sekine H,Okazaki K,Kato K,Alam MM,Shima H,Katsuoka F,Tsujita T,Suzuki N,Kobayashi A,Igarashi K,Yamamoto M,Motohashi H

更新日期：2018-08-15 00:00:00

abstract：:rRNA transcription in Xenopus laevis terminates near a 7-base-pair (bp) conserved sequence (T3 box) located 200 bp upstream of the site of transcription initiation for the adjacent gene promoter. We present evidence here that a 12-bp element containing the T3 box is an essential part of the terminator. Using an oocyte...

journal_title：Molecular and cellular biology

authors： Labhart P,Reeder RH

更新日期：1987-05-01 00:00:00