Evidence suggesting that the ARS elements associated with silencers of the yeast mating-type locus HML do not function as chromosomal DNA replication origins.

abstract：:The ribonucleoprotein enzyme telomerase is a specialized reverse transcriptase that synthesizes telomeric DNA by copying a template sequence within the telomerase RNA. Here we analyze the actions of telomerase from Tetrahymena thermophila assembled in vivo with mutated or wild-type telomerase RNA to define further the...

journal_title：Molecular and cellular biology

authors： Gilley D,Blackburn EH

更新日期：1996-01-01 00:00:00

abstract：:Mouse neuroblastoma Neuro-2A cells produce transforming growth factors during exponential growth in a defined hormone-free medium, which, on Bio-Gel columns in 1 M HAc, elute at a molecular size of 15 to 20 kilodaltons (kDa). These neuroblastoma-derived transforming growth factors have strong mitogenic activity, but t...

journal_title：Molecular and cellular biology

authors： van Zoelen EJ,van de Ven WJ,Franssen HJ,van Oostwaard TM,van der Saag PT,Heldin CH,de Laat SW

更新日期：1985-09-01 00:00:00

abstract：:GCN4 encodes a transcriptional activator of amino acid biosynthetic genes in Saccharomyces cerevisiae. The GCN3 product is a positive regulator required for increased synthesis of GCN4 protein in amino acid-starved cells. GCN3 appears to act indirectly by antagonizing GCD-encoded negative regulators of GCN4 expression...

journal_title：Molecular and cellular biology

authors： Hannig EM,Hinnebusch AG

更新日期：1988-11-01 00:00:00

abstract：:We have identified a new immunoglobulin heavy-chain enhancer element, designated pi, between the microE2 and microE3 elements. The pi enhancer element is transcriptionally active primarily during early stages of B-cell development but becomes virtually inactive during B-cell maturation at about the stage of immunoglob...

journal_title：Molecular and cellular biology

authors： Libermann TA,Baltimore D

更新日期：1993-10-01 00:00:00

abstract：:We recently identified Vav, the product of the vav proto-oncogene, as a guanine nucleotide exchange factor (GEF) for Ras. Vav is enzymatically activated by lymphocyte antigen receptor-coupled protein tyrosine kinases or independently by diglycerides. To further evaluate the physiological role of Vav, we assessed its G...

journal_title：Molecular and cellular biology

authors： Gulbins E,Coggeshall KM,Langlet C,Baier G,Bonnefoy-Berard N,Burn P,Wittinghofer A,Katzav S,Altman A

更新日期：1994-02-01 00:00:00

abstract：:Expression of the Cyp 2d-9 (steroid 16 alpha-hydroxylase) gene in mouse liver is male specific in such Mus musculus domesticus strains as FVB/N, whereas the corresponding P450 genes in the wild mouse species Mus spretus are not sex specific in their expression. These parental differences in the gene expressions were i...

journal_title：Molecular and cellular biology

authors： Sueyoshi T,Kobayashi R,Nishio K,Aida K,Moore R,Wada T,Handa H,Negishi M

更新日期：1995-08-01 00:00:00

abstract：:Phosphorylation is one of the key mechanisms that regulate centrosome biogenesis, spindle assembly, and cell cycle progression. However, little is known about centrosome-specific phosphorylation sites and their functional relevance. Here, we identified phosphoproteins of intact Drosophila melanogaster centrosomes and ...

journal_title：Molecular and cellular biology

authors： Habermann K,Mirgorodskaya E,Gobom J,Lehmann V,Müller H,Blümlein K,Deery MJ,Czogiel I,Erdmann C,Ralser M,von Kries JP,Lange BM

更新日期：2012-09-01 00:00:00

abstract：:We report that newly synthesized mRNA poly(A) tails are matured to precise lengths by the Pab1p-dependent poly(A) nuclease (PAN) of Saccharomyces cerevisiae. These results provide evidence for an initial phase of mRNA deadenylation that is required for poly(A) tail length control. In RNA 3'-end processing extracts lac...

journal_title：Molecular and cellular biology

authors： Brown CE,Sachs AB

更新日期：1998-11-01 00:00:00

abstract：:Transfection experiments with constructs containing various 5'-deleted fragments of the human lipoprotein lipase (LPL) promoter and the chloramphenicol acetyltransferase reporter gene revealed an LPL silencer element (LSE) in the region of nucleotides -225 to -81 of the LPL gene that functioned in Chinese hamster ovar...

journal_title：Molecular and cellular biology

authors： Tanuma Y,Nakabayashi H,Esumi M,Endo H

更新日期：1995-01-01 00:00:00

abstract：:The polypyrimidine tract near the 3' splice site is important for pre-mRNA splicing. Using pseudouridine incorporation and in vivo RNA-guided RNA pseudouridylation, we have identified two important uridines in the polypyrimidine tract of adenovirus pre-mRNA. Conversion of either uridine into pseudouridine leads to a s...

journal_title：Molecular and cellular biology

authors： Chen C,Zhao X,Kierzek R,Yu YT

更新日期：2010-09-01 00:00:00

abstract：:The oncogene product of the avian sarcoma virus CT10, P47gag-crk, contains the SH2, SH2', and SH3 domains and binds proteins in a phosphotyrosine (ptyr)-dependent manner. In this study, we have determined the region of P47gag-crk essential for binding to ptyr-containing proteins. Mutant P47gag-crk proteins expressed i...

journal_title：Molecular and cellular biology

authors： Matsuda M,Mayer BJ,Hanafusa H

更新日期：1991-03-01 00:00:00

abstract：:The phorbol ester receptor protein kinase C (PKC) gene family encodes essential mediators of eukaryotic cellular signals. Molecular dissection of their mechanisms of action has been limited in part by the lack of random mutagenesis approaches and by the complexity of signaling pathways in mammalian cells which involve...

journal_title：Molecular and cellular biology

authors： Riedel H,Su L,Hansen H

更新日期：1993-08-01 00:00:00

abstract：:In Drosophila and several other metazoan organisms, there are two genes that encode related but distinct homologs of ADA2-type transcriptional adaptors. Here we describe mutations of the two Ada2 genes of Drosophila melanogaster. By using mutant Drosophila lines, which allow the functional study of individual ADA2s, w...

journal_title：Molecular and cellular biology

authors： Pankotai T,Komonyi O,Bodai L,Ujfaludi Z,Muratoglu S,Ciurciu A,Tora L,Szabad J,Boros I

更新日期：2005-09-01 00:00:00

abstract：:The Saccharomyces cerevisiae cell cycle is arrested in G1 phase by the mating factor pathway. Genetic evidence has suggested that the G1 cyclins Cln1, Cln2, and Cln3 are targets of this pathway whose inhibition results in G1 arrest. Inhibition of Cln1- and Cln2-associated kinase activity by the mating factor pathway a...

journal_title：Molecular and cellular biology

authors： Jeoung DI,Oehlen LJ,Cross FR

更新日期：1998-01-01 00:00:00

abstract：:Expression of the anaphase-promoting complex/cyclosome (APC/C) inhibitor Emi1 is required for the accumulation of APC/C substrates crucial for DNA synthesis and mitotic entry. We show that in vivo Emi1 expression correlates with the proliferative status of the cellular compartment and that cells lacking Emi1 undergo c...

journal_title：Molecular and cellular biology

authors： Verschuren EW,Ban KH,Masek MA,Lehman NL,Jackson PK

更新日期：2007-11-01 00:00:00

abstract：:Mutations in the Saccharomyces cerevisiae alpha-factor receptor that lead to improved response to Saccharomyces kluyveri alpha-factor were identified and sequenced. Mutants were isolated from cells bearing randomly mutagenized receptor gene (STE2) plasmids by an in vivo screen. Five mutations lead to substitutions in ...

journal_title：Molecular and cellular biology

authors： Marsh L

更新日期：1992-09-01 00:00:00

abstract：:The promyelocytic leukemia zinc finger (PLZF) protein is a transcription factor disrupted in patients with t(11;17)(q23;q21)-associated acute promyelocytic leukemia. PLZF contains an N-terminal BTB/POZ domain which is required for dimerization, transcriptional repression, formation of high-molecular-weight DNA-protein...

journal_title：Molecular and cellular biology

authors： Melnick A,Ahmad KF,Arai S,Polinger A,Ball H,Borden KL,Carlile GW,Prive GG,Licht JD

更新日期：2000-09-01 00:00:00

abstract：:Although the primary function of U1 snRNA is to define the 5' donor site of an intron, it can also block the accumulation of a specific RNA transcript when it binds to a donor sequence within its terminal exon. This work was initiated to investigate if this property of U1 snRNA could be exploited as an effective metho...

journal_title：Molecular and cellular biology

authors： Beckley SA,Liu P,Stover ML,Gunderson SI,Lichtler AC,Rowe DW

更新日期：2001-04-01 00:00:00

abstract：:We have characterized a pathway for nuclear export of the glucocorticoid receptor (GR) in mammalian cells. This pathway involves the Ca2+ -binding protein calreticulin (CRT), which directly contacts the DNA binding domain (DBD) of GR and facilitates its delivery from the nucleus to the cytoplasm. In the present study,...

journal_title：Molecular and cellular biology

authors： Holaska JM,Black BE,Rastinejad F,Paschal BM

更新日期：2002-09-01 00:00:00

abstract：:Tax1, a potent activator of human T-cell lymphotropic virus type 1 (HTLV-1) transcription, has been shown to modulate expression of many cellular genes. Tax1 does not bind DNA directly but regulates transcription through protein-protein interactions with sequence-specific transcription factors. Using the yeast two-hyb...

journal_title：Molecular and cellular biology

authors： Pise-Masison CA,Dittmer J,Clemens KE,Brady JN

更新日期：1997-03-01 00:00:00

abstract：:We have previously shown that Saccharomyces cerevisiae Isw2 complex slides nucleosomes to remodel chromatin in vivo. Our data suggested a model in which Isw2 complex binds the histone octamer and DNA separately to generate the force necessary for nucleosome movement. Here we find that the histone H4 "basic patch" is t...

journal_title：Molecular and cellular biology

authors： Fazzio TG,Gelbart ME,Tsukiyama T

更新日期：2005-11-01 00:00:00

abstract：:Multidrug resistance (MDR) to different cytotoxic compounds in the yeast Saccharomyces cerevisiae can arise from overexpression of the Pdr5 (Sts1, Ydr1, or Lem1) ATP-binding cassette (ABC) multidrug transporter. We have raised polyclonal antibodies recognizing the yeast Pdr5 ABC transporter to study its biogenesis and...

journal_title：Molecular and cellular biology

authors： Egner R,Mahé Y,Pandjaitan R,Kuchler K

更新日期：1995-11-01 00:00:00

abstract：:The rapid nuclear translocation of signaling proteins upon stimulation is important for the regulation of de novo gene expression. We have studied the stimulated nuclear shuttling of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinases (MAPKs) and found that they translocate into the nucleus in a Ra...

journal_title：Molecular and cellular biology

authors： Zehorai E,Seger R

更新日期：2014-01-01 00:00:00

abstract：:Proteins of the Ras superfamily, Ras, Rac, Rho, and Cdc42, control the remodelling of the cortical actin cytoskeleton following growth factor stimulation. A major regulator of Ras, Ras-GAP, contains several structural motifs, including an SH3 domain and two SH2 domains, and there is evidence that they harbor a signall...

journal_title：Molecular and cellular biology

authors： Leblanc V,Tocque B,Delumeau I

更新日期：1998-09-01 00:00:00

abstract：:As a subunit of a ubiquitin ligase, Skp2 is implicated in facilitating cell cycle progression via degradation of various protein targets. We report here that Skp2 is rapidly degraded following cellular stimulation by the cytokine transforming growth factor beta (TGF-beta) and that this degradation stabilizes the cell ...

journal_title：Molecular and cellular biology

authors： Liu W,Wu G,Li W,Lobur D,Wan Y

更新日期：2007-04-01 00:00:00

abstract：:G proteins are molecular switches that control a wide variety of physiological functions, including neurotransmission, transcriptional activation, cell migration, cell growth. and proliferation. The ability of GTPases to participate in signaling events is determined by the ratio of GTP-bound to GDP-bound forms in the ...

journal_title：Molecular and cellular biology

authors： Xia C,Ma W,Stafford LJ,Liu C,Gong L,Martin JF,Liu M

更新日期：2003-04-01 00:00:00

abstract：:The Saccharomyces cerevisiae Cdc42p GTPase interacts with multiple regulators and downstream effectors through an approximately 25-amino-acid effector domain. Four effector domain mutations, Y32K, F37A, D38E, and Y40C, were introduced into Cdc42p and characterized for their effects on these interactions. Each mutant p...

journal_title：Molecular and cellular biology

authors： Richman TJ,Johnson DI

更新日期：2000-11-01 00:00:00

abstract：:We have previously proposed that the upstream regions of the human cardiac actin gene contain sequences that interact with muscle-specific factors with direct high-level transcription of this gene in differentiated muscle cells. In this study we showed that these factors already accumulate in the dividing myoblasts of...

journal_title：Molecular and cellular biology

authors： Minty A,Blau H,Kedes L

更新日期：1986-06-01 00:00:00

abstract：:Histone methylation is an important posttranslational modification that contributes to chromatin-based processes including transcriptional regulation, DNA repair, and epigenetic inheritance. In the budding yeast Saccharomyces cerevisiae, histone lysine methylation occurs on histone H3 lysines 4, 36, and 79, and its de...

journal_title：Molecular and cellular biology

authors： Klose RJ,Gardner KE,Liang G,Erdjument-Bromage H,Tempst P,Zhang Y

更新日期：2007-06-01 00:00:00

abstract：:The mitochondrial genome of trypanosomes is composed of thousands of topologically interlocked circular DNA molecules that form the kinetoplast DNA (kDNA). Most genes encoded by the kDNA require a posttranscriptional modification process called RNA editing to form functional mRNAs. Here, we show that alternative editi...

journal_title：Molecular and cellular biology

authors： Ochsenreiter T,Anderson S,Wood ZA,Hajduk SL

更新日期：2008-09-01 00:00:00