Repression of CDK1 and other genes with CDE and CHR promoter elements during DNA damage-induced G(2)/M arrest in human cells.

abstract：:Current amino acid and monosaccharide transport models are based on an assumption which equates the intracellular chemical activity of a solute with its concentration. This assumption was tested for alpha-aminoisobutyric acid and 3-O-methylglucose in a giant cell, the amphibian oocyte, by using recently developed cryo...

journal_title：Molecular and cellular biology

authors： Horowitz SB,Pearson TW

更新日期：1981-09-01 00:00:00

abstract：:Signaling downstream of mechanistic target of rapamycin complexes 1 and 2 (mTORC1 and mTORC2) controls specific and distinct aspects of insulin action and nutrient homeostasis in an interconnected and as yet unclear way. Mice lacking the mTORC1 substrate S6 kinase 1 (S6K1) maintain proper glycemic control with a high-...

journal_title：Molecular and cellular biology

authors： Treins C,Alliouachene S,Hassouna R,Xie Y,Birnbaum MJ,Pende M

更新日期：2012-10-01 00:00:00

abstract：:Copper is an essential cofactor of two mitochondrial enzymes: cytochrome c oxidase (COX) and Cu-Zn superoxide dismutase (Sod1p). Copper incorporation into these enzymes is facilitated by metallochaperone proteins which probably use copper from a mitochondrial matrix-localized pool. Here we describe a novel conserved m...

journal_title：Molecular and cellular biology

authors： Horn D,Al-Ali H,Barrientos A

更新日期：2008-07-01 00:00:00

abstract：:The repair of DNA single-strand breaks in mammalian cells is mediated by poly(ADP-ribose) polymerase 1 (PARP-1), DNA ligase IIIalpha, and XRCC1. Since these proteins are not found in lower eukaryotes, this DNA repair pathway plays a unique role in maintaining genome stability in more complex organisms. XRCC1 not only ...

journal_title：Molecular and cellular biology

authors： Leppard JB,Dong Z,Mackey ZB,Tomkinson AE

更新日期：2003-08-01 00:00:00

abstract：:Inhibition of mitosis by antimitotic drugs is thought to occur by destruction of microtubules, causing cells to arrest through the action of one or more mitotic checkpoints. We have patterned experiments in the yeast Saccharomyces cerevisiae after recent studies in mammalian cells that demonstrate the effectiveness of...

journal_title：Molecular and cellular biology

authors： Wang Y,Burke DJ

更新日期：1995-12-01 00:00:00

abstract：:The let-23 gene encodes a Caenorhabditis elegans homolog of the epidermal growth factor receptor (EGFR) necessary for vulval development. We have characterized a mutation of let-23 that activates the receptor and downstream signal transduction, leading to excess vulval differentiation. This mutation alters a conserved...

journal_title：Molecular and cellular biology

authors： Katz WS,Lesa GM,Yannoukakos D,Clandinin TR,Schlessinger J,Sternberg PW

更新日期：1996-02-01 00:00:00

abstract：:Type I interferons (IFNs) stimulate transcription through a latent heterotrimeric transcription factor composed of tyrosine-phosphorylated STAT1 and STAT2 and the DNA binding partner IRF9, with STAT2 contributing a critical transactivation domain. Human RVB1 and RVB2, which are highly conserved AAA(+) ATP binding prot...

journal_title：Molecular and cellular biology

authors： Gnatovskiy L,Mita P,Levy DE

更新日期：2013-10-01 00:00:00

abstract：:A cDNA encoding a novel Krüppel-type zinc finger protein, FKLF, was cloned from fetal globin-expressing human fetal erythroid cells. The deduced polypeptide sequence composed of 512 amino acids revealed that, like Sp1 and EKLF, FKLF has three contiguous zinc fingers at the near carboxyl-terminal end. A long amino-term...

journal_title：Molecular and cellular biology

authors： Asano H,Li XS,Stamatoyannopoulos G

更新日期：1999-05-01 00:00:00

abstract：:We analyzed sites of macronuclear telomere addition at a single genetic locus in Paramecium tetraurelia. We showed that in homozygous wild-type cells, differential genomic processing during macronuclear development resulted in the A surface antigen gene being located 8, 13, or 26 kilobases upstream from a macronuclear...

journal_title：Molecular and cellular biology

authors： Forney JD,Blackburn EH

更新日期：1988-01-01 00:00:00

abstract：:Here, we demonstrate that elevation of intracellular cyclic AMP (cAMP) in vascular endothelial cells (ECs) by either a direct activator of adenylyl cyclase or endogenous cAMP-mobilizing G protein-coupled receptors inhibited the tyrosine phosphorylation of STAT proteins by an interleukin 6 (IL-6) receptor trans-signali...

journal_title：Molecular and cellular biology

authors： Sands WA,Woolson HD,Milne GR,Rutherford C,Palmer TM

更新日期：2006-09-01 00:00:00

abstract：:The cys-3+ gene of Neurospora crassa encodes a bZIP (basic region-leucine zipper) regulatory protein that is essential for sulfur structural gene expression (e.g., ars-1+). Nuclear transcription assays confirmed that cys-3+ was under sulfur-regulated transcriptional control and that cys-3+ transcription was constituti...

journal_title：Molecular and cellular biology

authors： Paietta JV

更新日期：1992-04-01 00:00:00

abstract：:We have examined the in vivo sites of action for topoisomerases II in the 87A7 heat shock locus as a function of gene activity. When the hsp70 genes are induced, there is a dramatic redistribution of topoisomerase II in the locus which parallels many of the observed alterations in chromatin structure. In addition to c...

journal_title：Molecular and cellular biology

authors： Udvardy A,Schedl P

更新日期：1993-12-01 00:00:00

abstract：:We have previously described an isolated kinetoplast system from Crithidia fasciculata capable of ATP-dependent replication of kinetoplast DNA minicircles (L. Birkenmeyer and D.S. Ray, J. Biol. Chem. 261: 2362-2368, 1986). We present here the identification of two new minicircle species observed in short pulse-labelin...

journal_title：Molecular and cellular biology

authors： Sheline C,Melendy T,Ray DS

更新日期：1989-01-01 00:00:00

abstract：:Irradiation of simian virus 40 (SV40)-infected cells with low fluences of UV light (20 to 60 J/m2, inducing one to three pyrimidine dimers per SV40 genome) causes a dramatic inhibition of viral DNA replication. However, treatment of cells with UV radiation (20 J/m2) before infection with SV40 virus enhances the replic...

journal_title：Molecular and cellular biology

authors： Treger JM,Hauser J,Dixon K

更新日期：1988-06-01 00:00:00

abstract：:The Saccharomyces cerevisiae Cdc42p GTPase interacts with multiple regulators and downstream effectors through an approximately 25-amino-acid effector domain. Four effector domain mutations, Y32K, F37A, D38E, and Y40C, were introduced into Cdc42p and characterized for their effects on these interactions. Each mutant p...

journal_title：Molecular and cellular biology

authors： Richman TJ,Johnson DI

更新日期：2000-11-01 00:00:00

abstract：:ING1 is a type II tumor suppressor that affects cell growth, stress signaling, apoptosis, and DNA repair by altering chromatin structure and regulating transcription. Decreased ING1 expression is seen in several human cancers, and mislocalization has been noted in diverse types of cancer cells. Aberrant targeting may,...

journal_title：Molecular and cellular biology

authors： Gong W,Russell M,Suzuki K,Riabowol K

更新日期：2006-04-01 00:00:00

abstract：:The ATM protein has been implicated in pathways controlling cell cycle checkpoints, radiosensitivity, genetic instability, and aging. Expression of ATM fragments containing a leucine zipper motif in a human tumor cell line abrogated the S-phase checkpoint after ionizing irradiation and enhanced radiosensitivity and ch...

journal_title：Molecular and cellular biology

authors： Morgan SE,Lovly C,Pandita TK,Shiloh Y,Kastan MB

更新日期：1997-04-01 00:00:00

abstract：:Sequencing studies have shown that in somatic cells alternating runs of purines and pyrimidines are frequently associated with recombination crossover points. To test whether such sequences actually promote recombination, we have examined the effects of poly[d(pGpT).d(pApC)] and poly[d(pCpG).d(pCpG)] repeats on a homo...

journal_title：Molecular and cellular biology

authors： Bullock P,Miller J,Botchan M

更新日期：1986-11-01 00:00:00

abstract：:Growth factors and cyclic AMP (cAMP) are known to activate distinct intracellular signaling pathways. Fibroblast growth factor (FGF) activates ras-dependent kinase cascades, resulting in the activation of MAP kinases, whereas cAMP activates protein kinase A. In this study, we report that growth factors and cAMP act sy...

journal_title：Molecular and cellular biology

authors： Tan Y,Low KG,Boccia C,Grossman J,Comb MJ

更新日期：1994-11-01 00:00:00

abstract：:Galanin is a neuropeptide implicated in the regulation of feeding, reproduction, cognition, nociception, and seizure susceptibility. There are three known galanin receptor (GALR) subtypes (GALR1, GALR2, and GALR3), which bind to galanin with different affinities and have their own unique distributions, signaling mecha...

journal_title：Molecular and cellular biology

authors： Gottsch ML,Zeng H,Hohmann JG,Weinshenker D,Clifton DK,Steiner RA

更新日期：2005-06-01 00:00:00

abstract：:We have studied leucine transport in several Chinese hamster-human hybrid cell lines obtained by fusion of a temperature-sensitive line of Chinese hamster ovary cells, ts025C1, and normal human leukocytes. A hybrid cell line exhibiting a twofold increase in L-leucine uptake over that in the parental cell line was foun...

journal_title：Molecular and cellular biology

authors： Lobaton CD,Moreno A,Oxender DL

更新日期：1984-03-01 00:00:00

abstract：:Chromatin is regulated by cross talk among different histone modifications, which can occur between residues within the same tail or different tails in the nucleosome. The latter is referred to as trans-tail regulation, and the best-characterized example of this is the dependence of H3 methylation on H2B ubiquitylatio...

journal_title：Molecular and cellular biology

authors： Zheng S,Wyrick JJ,Reese JC

更新日期：2010-07-01 00:00:00

abstract：:Human CRK protein is a homolog of the chicken v-crk oncogene product and consists mostly of src homology region 2 (SH2) and SH3, which are shared by many proteins, in particular those involved in signal transduction. SH2 has been shown to bind specifically to phosphotyrosine-containing peptides. We report here that bo...

journal_title：Molecular and cellular biology

authors： Tanaka S,Hattori S,Kurata T,Nagashima K,Fukui Y,Nakamura S,Matsuda M

更新日期：1993-07-01 00:00:00

abstract：:Protein kinase activities were measured in Rous sarcoma virus-infected baby hamster kidney (BHK) cells that express v-src (BHK [v-src]) and compared with those of revertant and control BHK cells. We observed about a fivefold-higher tyrosine phosphorylating activity in BHK (v-src) cell extracts, which was due to src bu...

journal_title：Molecular and cellular biology

authors： Clinton GM,Roskoski R Jr

更新日期：1984-05-01 00:00:00

abstract：:A mutation (K38R) which specifically eliminates kinase activity was created in the Drosophila melanogaster ckI gene (doubletime [dbt]). In vitro, DBT protein carrying the K38R mutation (DBT(K/R)) interacted with Period protein (PER) but lacked kinase activity. In cell culture and in flies, DBT(K/R) antagonized the pho...

journal_title：Molecular and cellular biology

authors： Muskus MJ,Preuss F,Fan JY,Bjes ES,Price JL

更新日期：2007-12-01 00:00:00

abstract：:The CACCC-box binding protein erythroid Krüppel-like factor (EKLF/KLF1) is a master regulator that directs the expression of many important erythroid genes. We have previously shown that EKLF drives transcription of the gene for a second KLF, basic Krüppel-like factor, or KLF3. We have now tested the in vivo role of K...

journal_title：Molecular and cellular biology

authors： Funnell AP,Norton LJ,Mak KS,Burdach J,Artuz CM,Twine NA,Wilkins MR,Power CA,Hung TT,Perdomo J,Koh P,Bell-Anderson KS,Orkin SH,Fraser ST,Perkins AC,Pearson RC,Crossley M

更新日期：2012-08-01 00:00:00

abstract：:Axin is a recently identified protein encoded by the fused locus in mice that is required for normal vertebrate axis formation. We have defined a 25-amino-acid sequence in axin that comprises the glycogen synthase kinase 3beta (GSK-3beta) interaction domain (GID). In contrast to full-length axin, which has been shown ...

journal_title：Molecular and cellular biology

authors： Hedgepeth CM,Deardorff MA,Rankin K,Klein PS

更新日期：1999-10-01 00:00:00

abstract：:GCN5, a putative transcriptional adapter in humans and yeast, possesses histone acetyltransferase (HAT) activity which has been linked to GCN5's role in transcriptional activation in yeast. In this report, we demonstrate a functional interaction between human GCN5 (hGCN5) and the DNA-dependent protein kinase (DNA-PK) ...

journal_title：Molecular and cellular biology

authors： Barlev NA,Poltoratsky V,Owen-Hughes T,Ying C,Liu L,Workman JL,Berger SL

更新日期：1998-03-01 00:00:00

abstract：:Cells respond to environmental stimuli by fine-tuned regulation of gene expression. Here we investigated the dose-dependent modulation of gene expression at high temporal resolution in response to nutrient and stress signals in yeast. The GAL1 activity in cell populations is modulated in a well-defined range of galact...

journal_title：Molecular and cellular biology

authors： Rienzo A,Poveda-Huertes D,Aydin S,Buchler NE,Pascual-Ahuir A,Proft M

更新日期：2015-11-01 00:00:00

abstract：:The effect of atenolol, propranolol, trifluoperazine, and caffeine on the occurrence of meiotic diploid and disomic products in Saccharomyces cerevisiae was investigated. We demonstrated that atenolol, propranolol, and trifluoperazine reduce the occurrence of meiotic diploid products and that propranolol also slightly...

journal_title：Molecular and cellular biology

authors： Sora S,Bianchi M

更新日期：1982-11-01 00:00:00