Abstract:
:Transgenic mice containing a sheep metallothionein 1a-sheep growth hormone fusion gene exhibited low, tissue-specific basal levels of transgene mRNA expression, resulting in slightly elevated levels of circulating growth hormone that did not lead to a detectable increase in growth. After zinc stimulation, high levels of transgene mRNA expression were induced in a number of tissues; these levels correlated with increased levels of circulating growth hormone, resulting in growth increases of up to 1.5 times the levels of controls and unstimulated transgenic mice. After removal of the zinc stimulus, transgene expression and circulating growth hormone concentrations returned to basal levels. Additional evidence from the pattern of developmental expression of the transgene suggests that zinc is the main regulator of this promoter in mice. The demonstrated regulation and low basal level of expression of the sheep metallothionein 1a promoter make it a candidate for use in other mouse transgenic studies and for use in transgenic livestock, in which regulation of expression is essential.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Shanahan CM,Rigby NW,Murray JD,Marshall JT,Townrow CA,Nancarrow CD,Ward KAdoi
10.1128/mcb.9.12.5473subject
Has Abstractpub_date
1989-12-01 00:00:00pages
5473-9issue
12eissn
0270-7306issn
1098-5549journal_volume
9pub_type
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