Delineation of a novel pathway that regulates CD154 (CD40 ligand) expression.

abstract：:Major histocompatibility complex class II (MHCII) molecules play a pivotal role in the immune system because they direct the development and activation of CD4(+) T cells. There are three human MHCII isotypes, HLA-DR, HLA-DQ, and HLA-DP. Key transcription factors controlling MHCII genes have been identified by virtue o...

journal_title：Molecular and cellular biology

authors： Peretti M,Villard J,Barras E,Zufferey M,Reith W

更新日期：2001-09-01 00:00:00

abstract：:A 215-base-pair (bp) region of the mouse MOPC 41 kappa light-chain immunoglobulin gene enhancer has been analyzed for specific binding of lymphoid and nonlymphoid nuclear factors. Mobility shift assays with a series of overlapping DNA fragments have mapped DNA-binding sites for three unique factors. The B-cell-specifi...

journal_title：Molecular and cellular biology

authors： Currie RA,Roeder RG

更新日期：1989-10-01 00:00:00

abstract：:Previous studies have identified novel modifications in the core fold domain of histone H2B, but relatively little is known about the function of these putative histone modification sites. We have mutated core modifiable residues that are conserved in Saccharomyces cerevisiae histone H2B and characterized the effects ...

journal_title：Molecular and cellular biology

authors： Kyriss MN,Jin Y,Gallegos IJ,Sanford JA,Wyrick JJ

更新日期：2010-07-01 00:00:00

abstract：:Pax transactivation domain-interacting protein (PTIP, or PAXIP1) is required for mouse development and has been implicated in DNA damage responses and histone modification. However, the physiological roles of PTIP during embryogenesis remain unclear due to early embryonic lethality of null mutants. We describe two N-e...

journal_title：Molecular and cellular biology

authors： Mu W,Wang W,Schimenti JC

更新日期：2008-10-01 00:00:00

abstract：:Iron is an essential micronutrient for all eukaryotic organisms because it participates as a redox-active cofactor in many biological processes, including DNA replication and repair. Eukaryotic ribonucleotide reductases (RNRs) are Fe-dependent enzymes that catalyze deoxyribonucleoside diphosphate (dNDP) synthesis. We ...

journal_title：Molecular and cellular biology

authors： Sanvisens N,Romero AM,An X,Zhang C,de Llanos R,Martínez-Pastor MT,Bañó MC,Huang M,Puig S

更新日期：2014-09-01 00:00:00

abstract：:Both chemotaxis and phagocytosis depend upon actin-driven cell protrusions and cell membrane remodeling. While chemoattractant receptors rely upon canonical G-protein signaling to activate downstream effectors, whether such signaling pathways affect phagocytosis is contentious. Here, we report that Gαi nucleotide exch...

journal_title：Molecular and cellular biology

authors： Huang NN,Becker S,Boularan C,Kamenyeva O,Vural A,Hwang IY,Shi CS,Kehrl JH

更新日期：2014-11-15 00:00:00

abstract：:The gene encoding human hemojuvelin (HJV) is one of the genes that, when mutated, can cause juvenile hemochromatosis, an early-onset inherited disorder associated with iron overload. The 5' untranslated region of the human HJV mRNA has two upstream open reading frames (uORFs), with 28 and 19 codons formed by two upstr...

journal_title：Molecular and cellular biology

authors： Onofre C,Tomé F,Barbosa C,Silva AL,Romão L

更新日期：2015-04-01 00:00:00

abstract：:We investigated various parameters associated with the initial selection of mouse 3T6 cells for resistance to single concentrations of methotrexate and characterized resistant colonies for the presence of additional (amplified) copies of the dihydrofolate reductase gene. Our results indicate that the frequency of occu...

journal_title：Molecular and cellular biology

authors： Brown PC,Tlsty TD,Schimke RT

更新日期：1983-06-01 00:00:00

abstract：:Activation-induced deaminase (AID) is essential for class switch recombination and somatic hypermutation, and it has the ability to deaminate single-stranded DNA at cytidines. Mammalian class switch regions form R-loops upon transcription in the physiological orientation. The displaced DNA strand of an R-loop is force...

journal_title：Molecular and cellular biology

authors： Yu K,Roy D,Bayramyan M,Haworth IS,Lieber MR

更新日期：2005-03-01 00:00:00

abstract：:Recognition of gene promoters by RNA polymerase II is mediated by general transcription factor IID (TFIID), which has been thought to be a static complex and to play a passive role in the regulation of gene expression under the instruction of gene-specific transcription factors. Here we show that transforming growth f...

journal_title：Molecular and cellular biology

authors： Nakagawa T,Hosogane M,Nakagawa M,Morohoshi A,Funayama R,Nakayama K

更新日期：2018-02-12 00:00:00

abstract：:The c-fes locus encodes a 93-kDa non-receptor protein tyrosine kinase (Fes) that regulates the growth and differentiation of hematopoietic and vascular endothelial cells. Unique to Fes is a long N-terminal sequence with two regions of strong homology to coiled-coil oligomerization domains. We introduced leucine-to-pro...

journal_title：Molecular and cellular biology

authors： Cheng HY,Schiavone AP,Smithgall TE

更新日期：2001-09-01 00:00:00

abstract：:Cwc25 has previously been identified to associate with pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (nineteen complex, or NTC) involved in spliceosome activation. We show here that Cwc25 is neither tightly associated with NTC nor required for spliceosome activation but is required f...

journal_title：Molecular and cellular biology

authors： Chiu YF,Liu YC,Chiang TW,Yeh TC,Tseng CK,Wu NY,Cheng SC

更新日期：2009-11-01 00:00:00

abstract：:The human tumor necrosis factor alpha (TNF-alpha) gene is rapidly activated in response to multiple signals of stress and inflammation. We have identified transcription factors present in the TNF-alpha enhancer complex in vivo following ionophore stimulation (ATF-2/Jun and NFAT) and virus infection (ATF-2/Jun, NFAT, a...

journal_title：Molecular and cellular biology

authors： Falvo JV,Uglialoro AM,Brinkman BM,Merika M,Parekh BS,Tsai EY,King HC,Morielli AD,Peralta EG,Maniatis T,Thanos D,Goldfeld AE

更新日期：2000-03-01 00:00:00

abstract：:We have studied the abilities of different transactivation domains to stimulate the initiation and elongation (postinitiation) steps of RNA polymerase II transcription in vivo. Nuclear run-on and RNase protection analyses revealed three classes of activation domains: Sp1 and CTF stimulated initiation (type I); human i...

journal_title：Molecular and cellular biology

authors： Blau J,Xiao H,McCracken S,O'Hare P,Greenblatt J,Bentley D

更新日期：1996-05-01 00:00:00

abstract：:Chromosomal rearrangements associated with one Ty1 element in the iso-1-cytochrome c (CYC1) region of Saccharomyces cerevisiae yeast cells were examined. Most of the rearrangements were deletions of the three linked genes, CYC1, OSM1, and RAD7, and resulted from recombination involving the single Ty1 element and a sol...

journal_title：Molecular and cellular biology

authors： Downs KM,Brennan G,Liebman SW

更新日期：1985-12-01 00:00:00

abstract：:In eukaryotic cells, calcium entry across the cell surface activates nuclear gene expression, a process critically important for cell growth and differentiation, learning, and memory and immune cell functions. In immune cells, calcium entry occurs through store-operated Ca(2+) release-activated Ca(2+) (CRAC) channels,...

journal_title：Molecular and cellular biology

authors： Yeh YC,Parekh AB

更新日期：2015-04-01 00:00:00

abstract：:The Ets family transcription factor PU.1 is crucial for the regulation of hematopoietic development. Pu.1 is activated in hematopoietic stem cells and is expressed in mast cells, B cells, granulocytes, and macrophages but is switched off in T cells. Many of the transcription factors regulating Pu.1 have been identifie...

journal_title：Molecular and cellular biology

authors： Hoogenkamp M,Krysinska H,Ingram R,Huang G,Barlow R,Clarke D,Ebralidze A,Zhang P,Tagoh H,Cockerill PN,Tenen DG,Bonifer C

更新日期：2007-11-01 00:00:00

abstract：:We studied the process by which whd, a P-element insertion allele of the Drosophila melanogaster white locus, is replaced by its homolog in the presence of transposase. These events are interpreted as the result of double-strand gap repair following excision of the P transposon in whd. We used a series of alleles deri...

journal_title：Molecular and cellular biology

authors： Johnson-Schlitz DM,Engels WR

更新日期：1993-11-01 00:00:00

abstract：:We have previously reported that antibodies to phosphotyrosine recognize the phosphorylated forms of platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) receptors (Zippel et al., Biochim. Biophys. Acta 881:54-61, 1986, and Sturani et al., Biochem. Biophys. Res. Commun. 137:343-350, 1986). In this r...

journal_title：Molecular and cellular biology

authors： Sturani E,Zippel R,Toschi L,Morello L,Comoglio PM,Alberghina L

更新日期：1988-03-01 00:00:00

abstract：:Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts. Transcription factor Oct3/4 is an indispensable factor in the self-renewal of ES cells. In this study, we searched for a protein that would interact with Oct3/4 in ES cells and identified an orphan nuclear hormone receptor...

journal_title：Molecular and cellular biology

authors： Sun C,Nakatake Y,Akagi T,Ura H,Matsuda T,Nishiyama A,Koide H,Ko MS,Niwa H,Yokota T

更新日期：2009-08-01 00:00:00

abstract：:The intracellular signaling mechanisms underlying the pathogenesis of cardiac diseases are not fully understood. We report here that selective deletion of Shp2, an SH2-containing cytoplasmic tyrosine phosphatase, in striated muscle results in severe dilated cardiomyopathy in mice, leading to heart failure and prematur...

journal_title：Molecular and cellular biology

authors： Princen F,Bard E,Sheikh F,Zhang SS,Wang J,Zago WM,Wu D,Trelles RD,Bailly-Maitre B,Kahn CR,Chen Y,Reed JC,Tong GG,Mercola M,Chen J,Feng GS

更新日期：2009-01-01 00:00:00

abstract：:GCN4 encodes a transcriptional activator of amino acid biosynthetic genes in Saccharomyces cerevisiae. The GCN3 product is a positive regulator required for increased synthesis of GCN4 protein in amino acid-starved cells. GCN3 appears to act indirectly by antagonizing GCD-encoded negative regulators of GCN4 expression...

journal_title：Molecular and cellular biology

authors： Hannig EM,Hinnebusch AG

更新日期：1988-11-01 00:00:00

abstract：:We introduced a human beta-globin gene into murine erythroleukemia (MEL) cells by infection with recombinant retroviruses containing the complete genomic globin sequence. The beta-globin gene was correctly regulated during differentiation, steady-state mRNA levels being induced 5- to 30-fold after treatment of the cel...

journal_title：Molecular and cellular biology

authors： Cone RD,Weber-Benarous A,Baorto D,Mulligan RC

更新日期：1987-02-01 00:00:00

abstract：:The removal of oxidative damage from Saccharomyces cerevisiae DNA is thought to be conducted primarily through the base excision repair pathway. The Escherichia coli endonuclease III homologs Ntg1p and Ntg2p are S. cerevisiae N-glycosylase-associated apurinic/apyrimidinic (AP) lyases that recognize a wide variety of d...

journal_title：Molecular and cellular biology

authors： Swanson RL,Morey NJ,Doetsch PW,Jinks-Robertson S

更新日期：1999-04-01 00:00:00

abstract：:Tetraspanins are clustered in specific microdomains (named tetraspanin-enriched microdomains, or TERM) in the plasma membrane and regulate the functions of associated transmembrane receptors, including integrins and receptor tyrosine kinases. We have identified syntenin-1, a PDZ domain-containing protein, as a new com...

journal_title：Molecular and cellular biology

authors： Latysheva N,Muratov G,Rajesh S,Padgett M,Hotchin NA,Overduin M,Berditchevski F

更新日期：2006-10-01 00:00:00

abstract：:Serum induction of c-jun expression in HeLa cells requires a MEF2 site at -59 in the c-jun promoter. MEF2 sites, found in many muscle-specific enhancers, are bound by a family of transcription factors, MEF2A through -D, which are related to serum response factor in their DNA binding domains. We have found that MEF2D i...

journal_title：Molecular and cellular biology

authors： Han TH,Prywes R

更新日期：1995-06-01 00:00:00

abstract：:Xrn1p of Saccharomyces cerevisiae is a major cytoplasmic RNA turnover exonuclease which is evolutionarily conserved from yeasts to mammals. Deletion of the XRN1 gene causes pleiotropic phenotypes, which have been interpreted as indirect consequences of the RNA turnover defect. By sequence comparisons, we have identifi...

journal_title：Molecular and cellular biology

authors： Solinger JA,Pascolini D,Heyer WD

更新日期：1999-09-01 00:00:00

abstract：:Two members of the heat shock transcription factor (HSF) family, HSF1 and HSF2, both function as transcriptional activators of heat shock gene expression. However, the inducible DNA-binding activities of these two factors are regulated by distinct pathways. HSF1 is activated by heat shock and other forms of stress, wh...

journal_title：Molecular and cellular biology

authors： Sistonen L,Sarge KD,Morimoto RI

更新日期：1994-03-01 00:00:00

abstract：:Histone acetylation provides a switch between transcriptionally repressive and permissive chromatin. By regulating the chromatin structure at specific promoters, histone acetyltransferases (HATs) carry out important functions during differentiation and development of higher eukaryotes. HAT complexes are present in org...

journal_title：Molecular and cellular biology

authors： Guelman S,Suganuma T,Florens L,Weake V,Swanson SK,Washburn MP,Abmayr SM,Workman JL

更新日期：2006-10-01 00:00:00

abstract：:Substantial evidence supports a critical role for the activation of the Raf-1/MEK/mitogen-activated protein kinase pathway in oncogenic Ras-mediated transformation. For example, dominant negative mutants of Raf-1, MEK, and mitogen-activated protein kinase all inhibit Ras transformation. Furthermore, the observation th...

journal_title：Molecular and cellular biology

authors： Khosravi-Far R,White MA,Westwick JK,Solski PA,Chrzanowska-Wodnicka M,Van Aelst L,Wigler MH,Der CJ

更新日期：1996-07-01 00:00:00