Characterization of a vacuolar pyrophosphatase in Trypanosoma brucei and its localization to acidocalcisomes.

abstract：:A special group of mitochondrial outer membrane proteins spans the membrane once, exposing soluble domains to both sides of the membrane. These proteins are synthesized in the cytosol and then inserted into the membrane by an unknown mechanism. To identify proteins that are involved in the biogenesis of the single-spa...

journal_title：Molecular and cellular biology

authors： Papić D,Elbaz-Alon Y,Koerdt SN,Leopold K,Worm D,Jung M,Schuldiner M,Rapaport D

更新日期：2013-10-01 00:00:00

abstract：:Six4 is a member of the Six family genes, homologues of Drosophila melanogaster sine oculis. The gene is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles. To elucidate t...

journal_title：Molecular and cellular biology

authors： Ozaki H,Watanabe Y,Takahashi K,Kitamura K,Tanaka A,Urase K,Momoi T,Sudo K,Sakagami J,Asano M,Iwakura Y,Kawakami K

更新日期：2001-05-01 00:00:00

abstract：:In Saccharomyces cerevisiae, COX5a and COX5b encode two distinct forms of cytochrome c oxidase subunit V, Va and Vb, respectively. To determine the relative contribution of COX5a and COX5b to cytochrome c oxidase function, we have disrupted each gene. Cytochrome c oxidase activity levels and respiration rates of strai...

journal_title：Molecular and cellular biology

authors： Trueblood CE,Poyton RO

更新日期：1987-10-01 00:00:00

abstract：:TGF-alpha-PE40 is a hybrid protein composed of transforming growth factor-alpha (TGF-alpha) fused to a 40,000-dalton segment of Pseudomonas exotoxin A (PE40). This hybrid protein possesses the receptor-binding activity of TGF-alpha and the cell-killing properties of PE40. These properties enable TGF-alpha-PE40 to bind...

journal_title：Molecular and cellular biology

authors： Edwards GM,DeFeo-Jones D,Tai JY,Vuocolo GA,Patrick DR,Heimbrook DC,Oliff A

更新日期：1989-07-01 00:00:00

abstract：:Focal adhesion kinase (FAK) is a nonreceptor protein tyrosine kinase involved in integrin-mediated control of cell behavior. Following cell adhesion to components of the extracellular matrix, FAK becomes phosphorylated at multiple sites, including tyrosines 397, 576, and 577. Tyr-397 is an autophosphorylation site tha...

journal_title：Molecular and cellular biology

authors： Owen JD,Ruest PJ,Fry DW,Hanks SK

更新日期：1999-07-01 00:00:00

abstract：:Using in vitro replication assays, we compared native with salt-treated simian virus 40 minichromosomes isolated from infected cell nuclei. Minichromosomes from both preparations contain the full complement of nucleosomes, but salt treatment removes histone H1 and a fraction of nonhistone chromatin proteins. Both type...

journal_title：Molecular and cellular biology

authors： Krude T,de Maddalena C,Knippers R

更新日期：1993-02-01 00:00:00

abstract：:We have previously shown that activation of extracellular signal-regulated kinase (Erk) by epidermal growth factor (EGF) treatment was significantly decreased in mouse fibroblast cells expressing a mutant Shp-2 molecule lacking 65 amino acids in the SH2-N domain, Shp-2(Delta46-110). To address the molecular mechanism ...

journal_title：Molecular and cellular biology

authors： Shi ZQ,Yu DH,Park M,Marshall M,Feng GS

更新日期：2000-03-01 00:00:00

abstract：:The fucose alpha(1-->2) galactose beta structure is expressed by uterine epithelial cells in the mouse and has been implicated in blastocyst adhesion events thought to be required for murine implantation. Fucalpha(1-->2)Galbeta moieties and cognate fucosyltransferases are also expressed by epithelial cells of the male...

journal_title：Molecular and cellular biology

authors： Domino SE,Zhang L,Gillespie PJ,Saunders TL,Lowe JB

更新日期：2001-12-01 00:00:00

abstract：:Two Drosophila tumor necrosis factor receptor-associated factors (TRAF), DTRAF1 and DTRAF2, are proposed to have similar functions with their mammalian counterparts as a signal mediator of cell surface receptors. However, their in vivo functions and related signaling pathways are not fully understood yet. Here, we sho...

journal_title：Molecular and cellular biology

authors： Cha GH,Cho KS,Lee JH,Kim M,Kim E,Park J,Lee SB,Chung J

更新日期：2003-11-01 00:00:00

abstract：:Unintended DNA rearrangements in a differentiating lymphocyte can have severe, oncogenic consequences, but the mechanisms for avoiding pathogenic outcomes in V(D)J recombination are not well understood. The first level at which fidelity is instituted is in discrimination by the recombination proteins between authentic...

journal_title：Molecular and cellular biology

authors： Agard EA,Lewis SM

更新日期：2000-07-01 00:00:00

abstract：:"Neutropenia" refers to deficient numbers of neutrophils, the most abundant type of white blood cell. Two main forms of inherited neutropenia are cyclic neutropenia, in which neutrophil counts oscillate with a 21-day frequency, and severe congenital neutropenia, in which static neutropenia may evolve at times into leu...

journal_title：Molecular and cellular biology

authors： Salipante SJ,Rojas ME,Korkmaz B,Duan Z,Wechsler J,Benson KF,Person RE,Grimes HL,Horwitz MS

更新日期：2009-08-01 00:00:00

abstract：:Using the method of chromosomal walking, we have isolated a contiguous region of the Drosophila melanogaster X chromosome which corresponds to salivary gland chromosome bands 3C12 to 3D4. This five-band region contains approximately 100 kilobases of DNA, including those sequences comprising dunce, a gene which functio...

journal_title：Molecular and cellular biology

authors： Davis RL,Davidson N

更新日期：1984-02-01 00:00:00

abstract：:We find that immediately following transcript initiation, RNA polymerase II pauses at several locations even in the presence of relatively high (200 microM) levels of nucleoside triphosphates. Strong pauses with half-lives of >30 s were observed at +7, +18/19, and about +25 on the template used in these experiments. W...

journal_title：Molecular and cellular biology

authors： Pal M,Luse DS

更新日期：2002-01-01 00:00:00

abstract：:Yeast cells containing the recessive mas3 mutation display temperature-sensitive defects in both mitochondrial protein import and the cell division cycle. The import defect is characterized by two pools of mitochondrial precursors and a dramatically slower rate of posttranslational import. The effect of mas3 on cell c...

journal_title：Molecular and cellular biology

authors： Smith BJ,Yaffe MP

更新日期：1991-05-01 00:00:00

abstract：:SEC63 encodes a protein required for secretory protein translocation into the endoplasmic reticulum (ER) of Saccharomyces cerevisiae (J. A. Rothblatt, R. J. Deshaies, S. L. Sanders, G. Daum, and R. Schekman, J. Cell Biol. 109:2641-2652, 1989). Antibody directed against a recombinant form of the protein detects a 73-kD...

journal_title：Molecular and cellular biology

authors： Feldheim D,Rothblatt J,Schekman R

更新日期：1992-07-01 00:00:00

abstract：:The reciprocal expression of GATA-1 and GATA-2 during hematopoiesis is an important determinant of red blood cell development. Whereas Gata2 is preferentially transcribed early in hematopoiesis, elevated GATA-1 levels result in GATA-1 occupancy at sites upstream of the Gata2 locus and transcriptional repression. GATA-...

journal_title：Molecular and cellular biology

authors： Grass JA,Jing H,Kim SI,Martowicz ML,Pal S,Blobel GA,Bresnick EH

更新日期：2006-10-01 00:00:00

abstract：:B-lymphocyte development requires the basic helix-loop-helix proteins encoded by the E2A gene. In this study, the control mechanism of E2A was further explored by disruption of the E2A-related genes, E2-2 and HEB. In contrast to E2A, E2-2 and HEB are not essential for the establishment of the B-cell lineage. However, ...

journal_title：Molecular and cellular biology

authors： Zhuang Y,Cheng P,Weintraub H

更新日期：1996-06-01 00:00:00

abstract：:JUN transcription factors bind DNA as part of the AP-1 complex, regulate many cellular processes, and play a key role in oncogenesis. The three JUN proteins (c-JUN, JUNB, and JUND) can have both redundant and unique functions depending on the biological phenotype and cell type assayed. Mechanisms that allow this dynam...

journal_title：Molecular and cellular biology

authors： Selvaraj N,Budka JA,Ferris MW,Plotnik JP,Hollenhorst PC

更新日期：2015-01-01 00:00:00

abstract：:In cardiac ischemia-reperfusion injury, reactive oxygen species (ROS) generation and upregulation of the hypoxia-inducible protein BNIP3 result in mitochondrial permeabilization, but impairment in autophagic removal of damaged mitochondria provokes programmed cardiomyocyte death. BNIP3 expression and ROS generation re...

journal_title：Molecular and cellular biology

authors： Ma X,Liu H,Murphy JT,Foyil SR,Godar RJ,Abuirqeba H,Weinheimer CJ,Barger PM,Diwan A

更新日期：2015-03-01 00:00:00

abstract：:In the accompanying report (C. F. Webb, C. Das, S. Eaton, K. Calame, and P. Tucker, Mol. Cell. Biol. 11:5197-5205, 1991), we characterize B-cell-specific protein-DNA interactions at -500 and -200 bp upstream of the mu immunoglobulin heavy chain promoter whose abundances were increased by interleukin-5 plus antigen. Be...

journal_title：Molecular and cellular biology

authors： Webb CF,Das C,Eneff KL,Tucker PW

更新日期：1991-10-01 00:00:00

abstract：:Heterokaryons of Saccharomyces cerevisiae have been constructed utilizing the kar1-1 mutation, which prevents nuclear fusion during conjugation (J. Conde and G. Fink, Proc. Natl. Acad. Sci. U.S.A. 73:3651-3655, 1976). Each heterokaryon contained two haploid nuclei that were marked on several chromosomes. They segregat...

journal_title：Molecular and cellular biology

authors： Dutcher SK

更新日期：1981-03-01 00:00:00

abstract：:Wound healing in certain individuals leads to the development of keloid tumors which exhibit abnormal collagen metabolism and an increased abundance of extracellular matrix components. Comparison of fibronectin levels in fibroblasts derived from keloids and normal dermis revealed a relative increase in intracellular a...

journal_title：Molecular and cellular biology

authors： Babu M,Diegelmann R,Oliver N

更新日期：1989-04-01 00:00:00

abstract：:We have shown previously that transcription of the Drosophila homeotic gene Antennapedia results in four major RNA species which differ in long 5'- and 3'-untranslated sequences. The protein-coding portion of these transcripts, however, is located in exons common to all. Using RNase protection assays and further cDNA ...

journal_title：Molecular and cellular biology

authors： Stroeher VL,Gaiser JC,Garber RL

更新日期：1988-10-01 00:00:00

abstract：:The expression of c-ets2 is rapidly induced in a variety of myelomonocytic cell lines as they differentiate into macrophages. We find that constitutive expression of c-ets2 in the M1D+ myeloblast leukemic cell line (M1ets2) is sufficient to push these cells to a more differentiated state. The expression of several dif...

journal_title：Molecular and cellular biology

authors： Aperlo C,Pognonec P,Stanley ER,Boulukos KE

更新日期：1996-12-01 00:00:00

abstract：:We have analyzed the specific interaction of murine p53 with the consensus DNA-binding sequence 5'-AGACATGCCT-AGACATGCCT-3'. We used segments of p53 lacking the C-terminal, nonspecific DNA-binding domain because the presence of an autonomous nonspecific DNA-binding domain in wild-type p53 would complicate analysis of ...

journal_title：Molecular and cellular biology

authors： Wang Y,Schwedes JF,Parks D,Mann K,Tegtmeyer P

更新日期：1995-04-01 00:00:00

abstract：:Nucleoporins mediate transport of macromolecules across the nuclear pore complex, yet the function of many individual nucleoporins is largely unresolved. To address this question, we depleted cells of the cytoplasmic nucleoporins Nup214/CAN and Nup358/RanBP2 by RNA interference. Depletion of Nup214 resulted in codeple...

journal_title：Molecular and cellular biology

authors： Hutten S,Kehlenbach RH

更新日期：2006-09-01 00:00:00

abstract：:The eukaryotic Spt4-Spt5 heterodimer forms a higher-order complex with RNA polymerase II (and I) to regulate transcription elongation. Extensive genetic and functional data have revealed diverse roles of Spt4-Spt5 in coupling elongation with chromatin modification and RNA-processing pathways. A mechanistic understandi...

journal_title：Molecular and cellular biology

authors： Meyer PA,Li S,Zhang M,Yamada K,Takagi Y,Hartzog GA,Fu J

更新日期：2015-10-01 00:00:00

abstract：:We have determined the effects of a number of mutations in the small-t antigen mRNA intron on the alternative splicing pattern of the simian virus 40 early transcript. Expansion of the distance separating the small-t pre-mRNA lariat branch point and the shared large T-small t 3' splice site from 18 to 29 nucleotides (...

journal_title：Molecular and cellular biology

authors： Fu XY,Colgan JD,Manley JL

更新日期：1988-09-01 00:00:00

abstract：:B52, an essential SR protein of Drosophila melanogaster, stimulates pre-mRNA splicing in splicing-deficient mammalian S100 extracts. Surprisingly, mutant larvae depleted of B52 were found to be capable of splicing at least several pre-mRNAs tested (H. Z. Ring and J. T. Lis, Mol. Cell. Biol. 14:7499-7506, 1994). In a h...

journal_title：Molecular and cellular biology

authors： Hoffman BE,Lis JT

更新日期：2000-01-01 00:00:00

abstract：:The autonomously replicating rRNA genes (rDNA) in the somatic nucleus of Tetrahymena thermophila are maintained at a copy number of approximately 10(4) per nucleus. A mutant in which the replication properties of this molecule were altered was isolated and characterized. This mutation of inbred strain C3, named rmm4, ...

journal_title：Molecular and cellular biology

authors： Yaeger PC,Orias E,Shaiu WL,Larson DD,Blackburn EH

更新日期：1989-02-01 00:00:00