B-lymphocyte development is regulated by the combined dosage of three basic helix-loop-helix genes, E2A, E2-2, and HEB.

abstract：:Signal transducer and activator of transcription 6 (Stat6) and NF-kappaB are widely distributed transcription factors which are induced by different stimuli and bind to distinct DNA sequence motifs. Interleukin-4 (IL-4), which activates Stat6, synergizes with activators of NF-kappaB to induce IL-4-responsive genes, bu...

journal_title：Molecular and cellular biology

authors： Shen CH,Stavnezer J

更新日期：1998-06-01 00:00:00

abstract：:SCF complexes are E3 ubiquitin-protein ligases that mediate degradation of regulatory and signaling proteins and control G1/S cell cycle progression by degradation of G1 cyclins and the cyclin-dependent kinase inhibitor, Sic1. Interchangeable F-box proteins bind the core SCF components; each recruits a specific subset...

journal_title：Molecular and cellular biology

authors： Ivantsiv Y,Kaplun L,Tzirkin-Goldin R,Shabek N,Raveh D

更新日期：2006-03-01 00:00:00

abstract：:We examined the effects of 18 h of incubation of Chinese hamster ovary (CHO K1) cells with cycloheximide, hydroxyurea, and aphidicolin. Treatment of cells with cycloheximide alone at a concentration adequate to inhibit DNA synthesis to less than 10% of control was significantly less cytotoxic and clastogenic than trea...

journal_title：Molecular and cellular biology

authors： Sherwood SW,Schumacher RI,Schimke RT

更新日期：1988-07-01 00:00:00

abstract：:SR proteins are essential for pre-mRNA splicing in vitro, act early in the splicing pathway, and can influence alternative splice site choice. Here we describe the isolation of both dominant and loss-of-function alleles of B52, the gene for a Drosophila SR protein. The allele B52ED was identified as a dominant second-...

journal_title：Molecular and cellular biology

authors： Peng X,Mount SM

更新日期：1995-11-01 00:00:00

abstract：:TFIID is a multiprotein complex consisting of the TATA box binding protein and multiple tightly associated proteins (TAFIIs) that are required for transcription by selected activators. We previously reported cloning and partial characterization of human TAFII130 (hTAFII130). The central domain of hTAFII130 contains fo...

journal_title：Molecular and cellular biology

authors： Saluja D,Vassallo MF,Tanese N

更新日期：1998-10-01 00:00:00

abstract：:Eukaryotic translation initiation factor 4GI (eIF4GI) is an essential protein that is the target for translational regulation in many cellular processes and viral systems. It has been shown to function in both cap-dependent and cap-independent translation initiation by recruiting the 40S ribosomal subunit to the mRNA ...

journal_title：Molecular and cellular biology

authors： Byrd MP,Zamora M,Lloyd RE

更新日期：2002-07-01 00:00:00

abstract：:Various stresses activate the c-Jun N-terminal kinase (JNK), which is involved in the regulation of many aspects of cellular physiology, including apoptosis. Here we demonstrate that in contrast to UV irradiation, heat shock causes little or no stimulation of the JNK-activating kinase SEK1, while knocking out the SEK1...

journal_title：Molecular and cellular biology

authors： Meriin AB,Yaglom JA,Gabai VL,Zon L,Ganiatsas S,Mosser DD,Zon L,Sherman MY

更新日期：1999-04-01 00:00:00

abstract：:Each of two severely defective mouse kappa-chain genes has acquired a different intracisternal A particle (IAP) element within one of its introns. One IAP element generated 6-base-pair direct repeats upon insertion. In contrast, the other IAP element was not flanked by direct repeats and was missing a single nucleotid...

journal_title：Molecular and cellular biology

authors： Hawley RG,Shulman MJ,Hozumi N

更新日期：1984-12-01 00:00:00

abstract：:It is well accepted that for transcriptional silencing in budding yeast, the evolutionarily conserved lysine deacetylase Sir2, in concert with its partner proteins Sir3 and Sir4, establishes a chromatin structure that prevents RNA polymerase II (Pol II) transcription. However, the mechanism of repression remains contr...

journal_title：Molecular and cellular biology

authors： Gao L,Gross DS

更新日期：2008-06-01 00:00:00

abstract：:The RPC31 gene encoding the C31 subunit of Saccharomyces cerevisiae RNA polymerase C (III) has been isolated, starting from a C-terminal fragment cloned on a lambda gt11 library. It is unique on the yeast genome and lies on the left arm of chromosome XIV, very close to a NotI site. Its coding sequence perfectly matche...

journal_title：Molecular and cellular biology

authors： Mosrin C,Riva M,Beltrame M,Cassar E,Sentenac A,Thuriaux P

更新日期：1990-09-01 00:00:00

abstract：:We have previously described an isolated kinetoplast system from Crithidia fasciculata capable of ATP-dependent replication of kinetoplast DNA minicircles (L. Birkenmeyer and D.S. Ray, J. Biol. Chem. 261: 2362-2368, 1986). We present here the identification of two new minicircle species observed in short pulse-labelin...

journal_title：Molecular and cellular biology

authors： Sheline C,Melendy T,Ray DS

更新日期：1989-01-01 00:00:00

abstract：:The Ras-RAF-mitogen-activated protein kinase (Ras-RAF-MAPK) pathway is overactive in many cancers and in some developmental disorders. In one of those disorders, namely, Noonan syndrome, nine activating C-RAF mutations cluster around Ser(259), a regulatory site for inhibition by 14-3-3 proteins. We show that these mut...

journal_title：Molecular and cellular biology

authors： Molzan M,Schumacher B,Ottmann C,Baljuls A,Polzien L,Weyand M,Thiel P,Rose R,Rose M,Kuhenne P,Kaiser M,Rapp UR,Kuhlmann J,Ottmann C

更新日期：2010-10-01 00:00:00

abstract：:The expressed variant cell surface glycoprotein (VSG) gene of Trypanosoma brucei is located at the 3' end of a large, telomeric, polycistronic transcription unit or expression site. We show that the region 45 kb upstream of the VSG gene, in the expression site on a 1.5-Mb chromosome, contains at least two promoters th...

journal_title：Molecular and cellular biology

authors： Gottesdiener K,Chung HM,Brown SD,Lee MG,Van der Ploeg LH

更新日期：1991-05-01 00:00:00

abstract：:The precise expression of the N-myc proto-oncogene is essential for normal mammalian development, whereas altered N-myc gene regulation is known to be a determinant factor in tumor formation. Using transgenic mouse embryos, we show that N-myc sequences from kb -8.7 to kb +7.2 are sufficient to reproduce the N-myc embr...

journal_title：Molecular and cellular biology

authors： Potvin E,Beuret L,Cadrin-Girard JF,Carter M,Roy S,Tremblay M,Charron J

更新日期：2010-11-01 00:00:00

abstract：:The 3' noncoding region element (AUUUA)n specifically targets many short-lived mRNAs for degradation. Although the mechanism by which this sequence functions is not yet understood, a potential link between facilitated mRNA turnover and translation has been implied by the stabilization of cellular mRNAs in the presence...

journal_title：Molecular and cellular biology

authors： Aharon T,Schneider RJ

更新日期：1993-03-01 00:00:00

abstract：:A strong block to the elongation of nascent RNA transcripts by RNA polymerase II occurs in the 5' part of the mammalian c-fos proto-oncogene. In addition to the control of initiation, this mechanism contributes to transcriptional regulation of the gene. In vitro transcription experiments using nuclear extracts and pur...

journal_title：Molecular and cellular biology

authors： Mechti N,Piechaczyk M,Blanchard JM,Jeanteur P,Lebleu B

更新日期：1991-05-01 00:00:00

abstract：:Despite detailed knowledge of the components of the spindle assembly checkpoint, a molecular explanation of how cells die after prolonged spindle checkpoint activation, and thus how microtubule inhibitors and other antimitotic drugs ultimately elicit their lethal effects, has yet to emerge. Mitotically arrested cells ...

journal_title：Molecular and cellular biology

authors： Terrano DT,Upreti M,Chambers TC

更新日期：2010-02-01 00:00:00

abstract：:G(1)-specific transcription in the budding yeast Saccharomyces cerevisiae depends upon SBF and MBF. Whereas inactivation of SBF-regulated genes during the G(1)/S transition depends upon mitotic B-type cyclins, inactivation of MBF has been reported to involve multiple regulators, Nrm1 and Stb1. Nrm1 is a transcriptiona...

journal_title：Molecular and cellular biology

authors： de Bruin RA,Kalashnikova TI,Wittenberg C

更新日期：2008-11-01 00:00:00

abstract：:Tenomodulin (Tnmd) is a member of a new family of type II transmembrane glycoproteins. It is predominantly expressed in tendons, ligaments, and eyes, whereas the only other family member, chondromodulin I (ChM-I), is highly expressed in cartilage and at lower levels in the eye and thymus. The C-terminal extracellular ...

journal_title：Molecular and cellular biology

authors： Docheva D,Hunziker EB,Fässler R,Brandau O

更新日期：2005-01-01 00:00:00

abstract：:Heterokaryons of Saccharomyces cerevisiae have been constructed utilizing the kar1-1 mutation, which prevents nuclear fusion during conjugation (J. Conde and G. Fink, Proc. Natl. Acad. Sci. U.S.A. 73:3651-3655, 1976). Each heterokaryon contained two haploid nuclei that were marked on several chromosomes. They segregat...

journal_title：Molecular and cellular biology

authors： Dutcher SK

更新日期：1981-03-01 00:00:00

abstract：:The CDC8 gene, whose product is required for DNA replication in Saccharomyces cerevisiae, has been isolated on recombinant plasmids. The yeast vector YCp50 bearing the yeast ARS1, CEN4, and URA3 sequences, to provide for replication, stability, and selection, respectively, was used to prepare a recombinant plasmid poo...

journal_title：Molecular and cellular biology

authors： Kuo CL,Campbell JL

更新日期：1983-10-01 00:00:00

abstract：:Gap junctions are membrane channels that permit the interchange of ions and other low-molecular-weight molecules between adjacent cells. Rous sarcoma virus (RSV)-induced transformation is marked by an early and profound disruption of gap-junctional communication, suggesting that these membrane structures may serve as ...

journal_title：Molecular and cellular biology

authors： Crow DS,Beyer EC,Paul DL,Kobe SS,Lau AF

更新日期：1990-04-01 00:00:00

abstract：:The TAR RNA binding Protein, TRBP, inhibits the activity of the interferon-induced protein kinase R (PKR), whereas the PKR activator, PACT, activates its function. TRBP and PACT also bind to each other through their double-stranded RNA binding domains (dsRBDs) and their Medipal domains, which may influence their activ...

journal_title：Molecular and cellular biology

authors： Daher A,Laraki G,Singh M,Melendez-Peña CE,Bannwarth S,Peters AH,Meurs EF,Braun RE,Patel RC,Gatignol A

更新日期：2009-01-01 00:00:00

abstract：:Previously, we described a retrovirus vector that contained two genes: a 5' gene under transcriptional control of the viral long terminal repeat and a 3' gene under transcriptional control of the herpes simplex virus thymidine kinase promoter. By using a biological assay, we found that expression of the 5' gene is sup...

journal_title：Molecular and cellular biology

authors： Emerman M,Temin HM

更新日期：1986-03-01 00:00:00

abstract：:Transcription initiation factor TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. To further understand the role of the 85-kDa TFIID subunit (p85), we have cloned the corresponding cDNA with a probe based on an amino acid sequence of t...

journal_title：Molecular and cellular biology

authors： Kokubo T,Gong DW,Yamashita S,Takada R,Roeder RG,Horikoshi M,Nakatani Y

更新日期：1993-12-01 00:00:00

abstract：:Expression of the reaper gene (rpr) correlates with the initiation of apoptosis in Drosophila melanogaster. Transient expression of rpr in the lepidopteran SF-21 cell line induced apoptosis displaying nuclear condensation and fragmentation, oligonucleosomal ladder formation, cell surface blebbing, and apoptotic body f...

journal_title：Molecular and cellular biology

authors： Vucic D,Seshagiri S,Miller LK

更新日期：1997-02-01 00:00:00

abstract：:The active beta-globin genes in chicken erythrocytes, like all active genes, reside in large chromatin domains which are preferentially sensitive to digestion by DNase I. We have recently proposed that the special structure of chromatin in active domains is maintained by torsional stress in the DNA (Villeponteau et al...

journal_title：Molecular and cellular biology

authors： Villeponteau B,Martinson HG

更新日期：1987-05-01 00:00:00

abstract：:We have previously described a 160-bp enhancer (BCE-1) in the bovine beta-casein gene that is activated in the presence of prolactin and extracellular matrix (ECM). Here we report the characterization of the enhancer by deletion and site-directed mutagenesis, electrophoretic mobility shift analysis, and in vivo footpr...

journal_title：Molecular and cellular biology

authors： Myers CA,Schmidhauser C,Mellentin-Michelotti J,Fragoso G,Roskelley CD,Casperson G,Mossi R,Pujuguet P,Hager G,Bissell MJ

更新日期：1998-04-01 00:00:00

abstract：:DNA in the polyploid macronucleus of the ciliated protozoan Tetrahymena thermophila contains the modified base N6-methyladenine. We identified two GATC sites which are methylated in most or all of the 45 copies of the macronuclear genome. One site is 2 kilobases 5' to the histone H4-I gene, and the other is 5 kilobase...

journal_title：Molecular and cellular biology

authors： Harrison GS,Findly RC,Karrer KM

更新日期：1986-07-01 00:00:00

abstract：:We have analyzed the effects of transformation by Rous sarcoma virus on expression of types I and II collagen and fibronectin genes in vertebral chondrocytes and compared them with expression of these genes in skin fibroblasts. Transformed chondrocytes display a dramatically decreased amount of type II collagen RNA, w...

journal_title：Molecular and cellular biology

authors： Allebach ES,Boettiger D,Pacifici M,Adams SL

更新日期：1985-05-01 00:00:00