Abstract:
:Various stresses activate the c-Jun N-terminal kinase (JNK), which is involved in the regulation of many aspects of cellular physiology, including apoptosis. Here we demonstrate that in contrast to UV irradiation, heat shock causes little or no stimulation of the JNK-activating kinase SEK1, while knocking out the SEK1 gene completely blocks heat-induced JNK activation. Therefore, we tested whether heat shock activates JNK via inhibition of JNK dephosphorylation. The rate of JNK dephosphorylation in unstimulated cells was high, and exposure to UV irradiation, osmotic shock, interleukin-1, or anisomycin did not affect this process. Conversely, exposure of cells to heat shock and other protein-damaging conditions, including ethanol, arsenite, and oxidative stress, strongly reduced the rate of JNK dephosphorylation. Under these conditions, we did not observe any effects on dephosphorylation of the homologous p38 kinase, suggesting that suppression of dephosphorylation is specific to JNK. Together, these data indicate that activation of JNK by protein-damaging treatments is mediated primarily by inhibition of a JNK phosphatase(s). Elevation of cellular levels of the major heat shock protein Hsp72 inhibited a repression of JNK dephosphorylation by these stressful treatments, which explains recent reports of the suppression of JNK activation by Hsp72.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Meriin AB,Yaglom JA,Gabai VL,Zon L,Ganiatsas S,Mosser DD,Zon L,Sherman MYdoi
10.1128/mcb.19.4.2547subject
Has Abstractpub_date
1999-04-01 00:00:00pages
2547-55issue
4eissn
0270-7306issn
1098-5549journal_volume
19pub_type
杂志文章abstract::We have cloned and characterized the gene encoding the late histone H1-beta subtype from the sea urchin Strongylocentrotus purpuratus. The gene contains all of the upstream sequence homologies previously seen in late H1-gamma genes. The expression of H1-beta mRNA is coordinated with that of H1-gamma mRNA, and like H1-...
journal_title:Molecular and cellular biology
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journal_title:Molecular and cellular biology
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更新日期:1997-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
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更新日期:2010-09-01 00:00:00
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更新日期:1984-12-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.25.13.5648-5663.2005
更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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更新日期:2000-02-01 00:00:00
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pub_type: 杂志文章,收录出版
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更新日期:2016-11-28 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2007-11-01 00:00:00
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更新日期:2015-06-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1988-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1995-04-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1990-12-01 00:00:00