Nuclear envelope localization of an adenovirus tumor antigen maintains the integrity of cellular DNA.

abstract：:macroH2A histone variants have been implicated to function in gene silencing by several studies, including ones showing a preferential association of macroH2A on the inactive X chromosome. To examine macroH2A function in vivo, we knocked out macroH2A1. macroH2A1 knockout mice are viable and fertile. A broad screen of ...

journal_title：Molecular and cellular biology

authors： Changolkar LN,Costanzi C,Leu NA,Chen D,McLaughlin KJ,Pehrson JR

更新日期：2007-04-01 00:00:00

abstract：:Entry into mitosis requires the activity of the Cdc2 kinase. Cdc2 associates with the B-type cyclins, and the Cdc2-cyclin B heterodimer is in turn regulated by phosphorylation. Phosphorylation of threonine 161 is required for the Cdc2-cyclin B complex to be catalytically active, whereas phosphorylation of threonine 14...

journal_title：Molecular and cellular biology

authors： Liu F,Stanton JJ,Wu Z,Piwnica-Worms H

更新日期：1997-02-01 00:00:00

abstract：:Activating mutations in KEAP1-NRF2 are frequently found in tumors of the lung, esophagus, and liver, where they are associated with aggressive growth, resistance to cancer therapies, and low overall survival. Despite the fact that NRF2 is a validated driver of tumorigenesis and chemotherapeutic resistance, there are c...

journal_title：Molecular and cellular biology

authors： Baird L,Suzuki T,Takahashi Y,Hishinuma E,Saigusa D,Yamamoto M

更新日期：2020-10-26 00:00:00

abstract：:Avian leukosis virus (ALV)-induced neoplasias are commonly found associated with integrations of proviral DNA in proximity to the myc gene. However, studies suggest that other genetic events are necessary for the complete neoplastic phenotype. A cell line (HP46) derived from an ALV-induced tumor has been analyzed and ...

journal_title：Molecular and cellular biology

authors： Kabrun N,Bumstead N,Hayman MJ,Enrietto PJ

更新日期：1990-09-01 00:00:00

abstract：:Coordination of skeletal muscle growth and metabolism with nutrient availability is critical for metabolic homeostasis. To establish the role of insulin-like signaling in this process, we used muscle creatine kinase (MCK)-Cre to disrupt expression of insulin receptor substrates Irs1 and Irs2 in mouse skeletal/cardiac ...

journal_title：Molecular and cellular biology

authors： Long YC,Cheng Z,Copps KD,White MF

更新日期：2011-02-01 00:00:00

abstract：:CAP, a protein from Saccharomyces cerevisiae that copurifies with adenylyl cyclase, appears to be required for yeast cells to be fully responsive to RAS proteins. CAP also appears to be required for normal cell morphology and responsiveness to nutrient deprivation and excess. We describe here a molecular and phenotypi...

journal_title：Molecular and cellular biology

authors： Gerst JE,Ferguson K,Vojtek A,Wigler M,Field J

更新日期：1991-03-01 00:00:00

abstract：:cDNAs for the murine lyn protein tyrosine kinase gene were cloned from mouse bone marrow-derived monocytic cells. Comparison of the human and murine genes demonstrated a 94% homology in peptide sequence. Comparable to the human gene, murine lyn was found to be expressed in myeloid and B-lymphoid lineage cells. During ...

journal_title：Molecular and cellular biology

authors： Yi TL,Bolen JB,Ihle JN

更新日期：1991-05-01 00:00:00

abstract：:We investigated the interaction of phospholipase C-gamma (PLC-gamma) with wild-type and mutant forms of the platelet-derived growth factor (PDGF) beta-receptor both in vivo and in vitro. After PDGF treatment of CHO cell lines expressing wild-type or either of two mutant (delta Ki and Y825F) PDGF receptors, PLC-gamma b...

journal_title：Molecular and cellular biology

authors： Morrison DK,Kaplan DR,Rhee SG,Williams LT

更新日期：1990-05-01 00:00:00

abstract：:Regulated transcription of class II genes of the yeast Saccharomyces cerevisiae requires the diverse functions of mediator complex. In particular, MED6 is essential for activated transcription from many class II promoters, suggesting that it functions as a key player in the relay of activator signals to the basal tran...

journal_title：Molecular and cellular biology

authors： Lee YC,Kim YJ

更新日期：1998-09-01 00:00:00

abstract：:Spinal muscular atrophy (SMA) is an inherited motor neuron disease caused by homozygous loss of the Survival Motor Neuron 1 (SMN1) gene. In the absence of SMN1, inefficient inclusion of exon 7 in transcripts from the nearly identical SMN2 gene results in ubiquitous SMN decrease but selective motor neuron degeneration....

journal_title：Molecular and cellular biology

authors： Ruggiu M,McGovern VL,Lotti F,Saieva L,Li DK,Kariya S,Monani UR,Burghes AH,Pellizzoni L

更新日期：2012-01-01 00:00:00

abstract：:Translation initiation plays an important role in cell growth, proliferation, and survival. The translation initiation factor eIF4B (eukaryotic initiation factor 4B) stimulates the RNA helicase activity of eIF4A in unwinding secondary structures in the 5' untranslated region (5'UTR) of the mRNA in vitro. Here, we stud...

journal_title：Molecular and cellular biology

authors： Shahbazian D,Parsyan A,Petroulakis E,Topisirovic I,Martineau Y,Gibbs BF,Svitkin Y,Sonenberg N

更新日期：2010-03-01 00:00:00

abstract：:CHIP is a cochaperone of Hsp70 that inhibits Hsp70-dependent refolding in vitro. However, the effect of altered expression of CHIP on the fate of unfolded proteins in mammalian cells has not been determined. Surprisingly, we found that overexpression of CHIP in fibroblasts increased the refolding of proteins after the...

journal_title：Molecular and cellular biology

authors： Kampinga HH,Kanon B,Salomons FA,Kabakov AE,Patterson C

更新日期：2003-07-01 00:00:00

abstract：:An electrophoretic mobility shift assay was used to characterize interactions of nuclear proteins with a DNA segment in the enhancer element of the leukemogenic murine retrovirus SL3-3. Mutation of a DNA sequence of the 5'-TGTGG-3' type decreased transcription in vivo specifically in T-lymphocyte cell lines. Extracts ...

journal_title：Molecular and cellular biology

authors： Thornell A,Hallberg B,Grundström T

更新日期：1988-04-01 00:00:00

abstract：:Multiple organ systems require epithelial barriers for normal function, and barrier loss is a hallmark of diseases ranging from inflammation to epithelial cancers. However, the molecular processes regulating epithelial barrier maturation are not fully elucidated. After contact, epithelial cells undergo size-reductive ...

journal_title：Molecular and cellular biology

authors： Bolinger MT,Ramshekar A,Waldschmidt HV,Larsen SD,Bewley MC,Flanagan JM,Antonetti DA

更新日期：2016-07-14 00:00:00

abstract：:Ligand-induced dimerization and transphosphorylation are thought to be important events by which receptor tyrosine kinases generate cellular signals. We have investigated the ability of signalling-defective, truncated fibroblast growth factor (FGF) receptors (FGFR-1 and FGFR-2) to block the FGF response in cells that ...

journal_title：Molecular and cellular biology

authors： Li Y,Basilico C,Mansukhani A

更新日期：1994-11-01 00:00:00

abstract：:We set up an alternative splicing system in vitro in which the relative amounts of two spliced RNAs, one containing and the other lacking a particular exon, were directly proportional to the length of an inverted repeat inserted into the flanking introns. We then used the system to measure the effect of intramolecular...

journal_title：Molecular and cellular biology

authors： Solnick D,Lee SI

更新日期：1987-09-01 00:00:00

abstract：:The gene encoding the SNF5/Ini1 core subunit of the SWI/SNF chromatin remodeling complex is a tumor suppressor in humans and mice, with an essential role in early embryonic development. To investigate further the function of this gene, we have generated a Cre/lox-conditional mouse line. We demonstrate that Snf5 deleti...

journal_title：Molecular and cellular biology

authors： Klochendler-Yeivin A,Picarsky E,Yaniv M

更新日期：2006-04-01 00:00:00

abstract：:We find that immediately following transcript initiation, RNA polymerase II pauses at several locations even in the presence of relatively high (200 microM) levels of nucleoside triphosphates. Strong pauses with half-lives of >30 s were observed at +7, +18/19, and about +25 on the template used in these experiments. W...

journal_title：Molecular and cellular biology

authors： Pal M,Luse DS

更新日期：2002-01-01 00:00:00

abstract：:We investigated two phenotypically distinct types of diphtheria toxin-resistant mutants of Chinese hamster cells and compared their resistance with that of naturally resistant mouse cells. All are resistant due to a defect in the process of internalization and delivery of toxin to its target in the cytosol, elongation...

journal_title：Molecular and cellular biology

authors： Didsbury JR,Moehring JM,Moehring TJ

更新日期：1983-07-01 00:00:00

abstract：:The 12p13 ETV6 (TEL) gene is frequently targeted by chromosomal translocations in human malignancies, resulting in the formation of oncogenic ETV6 gene fusions. Many of the known partner genes encode protein tyrosine kinases (PTKs), generating fusion proteins that function as chimeric PTKs. ETV6-NTRK3 (EN), comprised ...

journal_title：Molecular and cellular biology

authors： Tognon CE,Mackereth CD,Somasiri AM,McIntosh LP,Sorensen PH

更新日期：2004-06-01 00:00:00

abstract：:We have developed a system to study mutations that affect xanthine-guanine phosphoribosyltransferase gene (gpt) expression in hypoxanthine-guanine phosphoribosyltransferase-deficient CHO cells that have been transformed by the plasmid vector pSV2gpt. One isolated transformant, designated AS52, carries a single copy of...

journal_title：Molecular and cellular biology

authors： Tindall KR,Stankowski LF Jr,Machanoff R,Hsie AW

更新日期：1984-07-01 00:00:00

abstract：:Nuclear hormone receptor coregulator (NRC) is a 2,063-amino-acid coregulator of nuclear hormone receptors and other transcription factors (e.g., c-Fos, c-Jun, and NF-kappaB). We and others have generated C57BL/6-129S6 hybrid (C57/129) NRC(+/-) mice that appear outwardly normal and grow and reproduce. In contrast, homo...

journal_title：Molecular and cellular biology

authors： Mahajan MA,Das S,Zhu H,Tomic-Canic M,Samuels HH

更新日期：2004-06-01 00:00:00

abstract：:Wnt signaling plays an important role in the regulation of bone formation and bone mass. The mechanisms that regulate canonical Wnt signaling in osteoblasts are not fully understood. We show here a novel mechanism by which the adhesion molecule N-cadherin interacts with the Wnt coreceptor LRP5 and regulates canonical ...

journal_title：Molecular and cellular biology

authors： Haÿ E,Laplantine E,Geoffroy V,Frain M,Kohler T,Müller R,Marie PJ

更新日期：2009-02-01 00:00:00

abstract：:Multiple biological functions have been ascribed to the Ras-related G protein R-Ras. These include the ability to transform NIH 3T3 fibroblasts, the promotion of cell adhesion, and the regulation of apoptotic responses in hematopoietic cells. To investigate the signaling mechanisms responsible for these biological phe...

journal_title：Molecular and cellular biology

authors： Osada M,Tolkacheva T,Li W,Chan TO,Tsichlis PN,Saez R,Kimmelman AC,Chan AM

更新日期：1999-09-01 00:00:00

abstract：:TEL1 is important in Saccharomyces cerevisiae telomere maintenance, and its kinase activity is required. Tel1p associates with telomeres in vivo, is enriched at short telomeres, and enhances the binding of telomerase components to short telomeres. However, it is unclear how the kinase activity and telomere association...

journal_title：Molecular and cellular biology

authors： Ma Y,Greider CW

更新日期：2009-09-01 00:00:00

abstract：:The protein kinase Akt plays a critical role in heart function and is activated by phosphorylation of threonine 308 (T308) and serine 473 (S473). While phosphoinositide-dependent kinase 1 (PDK1) is responsible for Akt T308 phosphorylation, the identities of the kinases for Akt S473 phosphorylation in the heart remain ...

journal_title：Molecular and cellular biology

authors： Zhao X,Lu S,Nie J,Hu X,Luo W,Wu X,Liu H,Feng Q,Chang Z,Liu Y,Cao Y,Sun H,Li X,Hu Y,Yang Z

更新日期：2014-06-01 00:00:00

abstract：:In the mouse mammary tumor virus promoter, a tandem of octamer motifs, recognized by ubiquitous and tissue-restricted Oct transcription factors, is located upstream of the TATA box and next to a binding site for the transcription factor nuclear factor I (NF-I). Their function was investigated with mutant long terminal...

journal_title：Molecular and cellular biology

authors： Buetti E

更新日期：1994-02-01 00:00:00

abstract：:We delimited sequences necessary for in vivo expression of the Drosophila melanogaster dopa decarboxylase gene Ddc. The expression of in vitro-altered genes was assayed following germ line integration via P-element vectors. Sequences between -209 and -24 were necessary for normally regulated expression, although genes...

journal_title：Molecular and cellular biology

authors： Hirsh J,Morgan BA,Scholnick SB

更新日期：1986-12-01 00:00:00

abstract：:We investigated the role of RNA polymerase II (pol II) carboxy-terminal domain (CTD) phosphorylation in pre-mRNA processing coupled and uncoupled from transcription in Xenopus oocytes. Inhibition of CTD phosphorylation by the kinase inhibitors 5,6-dichloro-1beta-D-ribofuranosyl-benzimidazole and H8 blocked transcripti...

journal_title：Molecular and cellular biology

authors： Bird G,Zorio DA,Bentley DL

更新日期：2004-10-01 00:00:00

abstract：:Different models have been proposed explaining how eukaryotic gene transcription is terminated. Recently, Nsi1, a factor involved in silencing of ribosomal DNA (rDNA), was shown to be required for efficient termination of rDNA transcription by RNA polymerase I (Pol I) in the yeast Saccharomyces cerevisiae. Nsi1 contai...

journal_title：Molecular and cellular biology

authors： Merkl P,Perez-Fernandez J,Pilsl M,Reiter A,Williams L,Gerber J,Böhm M,Deutzmann R,Griesenbeck J,Milkereit P,Tschochner H

更新日期：2014-10-01 00:00:00