Abstract:
:We delimited sequences necessary for in vivo expression of the Drosophila melanogaster dopa decarboxylase gene Ddc. The expression of in vitro-altered genes was assayed following germ line integration via P-element vectors. Sequences between -209 and -24 were necessary for normally regulated expression, although genes lacking these sequences could be expressed at 10 to 50% of wild-type levels at specific developmental times. These genes showed components of normal developmental expression, which suggests that they retain some regulatory elements. All Ddc genes lacking the normal immediate 5'-flanking sequences were grossly deficient in larval central nervous system expression. Thus, this upstream region must contain at least one element necessary for this expression. A mutated Ddc gene without a normal TATA boxlike sequence used the normal RNA start points, indicating that this sequences is not required for start point specificity.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Hirsh J,Morgan BA,Scholnick SBdoi
10.1128/mcb.6.12.4548subject
Has Abstractpub_date
1986-12-01 00:00:00pages
4548-57issue
12eissn
0270-7306issn
1098-5549journal_volume
6pub_type
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更新日期:1999-11-01 00:00:00
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更新日期:1998-08-01 00:00:00
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