Abstract:
:Heteroduplexes formed between DNA strands derived from different homologous chromosomes are an intermediate in meiotic crossing over in the yeast Saccharomyces cerevisiae and other eucaryotes. A heteroduplex formed between wild-type and mutant genes will contain a base pair mismatch; failure to repair this mismatch will lead to postmeiotic segregation (PMS). By analyzing the frequency of PMS for various mutant alleles in the yeast HIS4 gene, we showed that C/C mismatches were inefficiently repaired relative to all other point mismatches. These other mismatches (G/G, G/A, T/T, A/A, T/C, C/A, A/A, and T/G) were repaired with approximately the same efficiency. We found that in spores with unrepaired mismatches in heteroduplexes, the nontranscribed strand of the HIS4 gene was more frequently donated than the transcribed strand. In addition, the direction of repair for certain mismatches was nonrandom.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Detloff P,Sieber J,Petes TDdoi
10.1128/mcb.11.2.737subject
Has Abstractpub_date
1991-02-01 00:00:00pages
737-45issue
2eissn
0270-7306issn
1098-5549journal_volume
11pub_type
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