Abstract:
:The Neurospora crassa arg-2 upstream open reading frame (uORF) plays a role in negative arginine-specific translational regulation. Primer extension inhibition analyses of arg-2 uORF-containing RNA translated in a cell-free system in which arginine-specific regulation was retained revealed "toeprints" corresponding to ribosomes positioned at the uORF initiation and termination codons and at the downstream initiation codon. At high arginine concentrations, the toeprint signal corresponding to ribosomes at the uORF termination codon rapidly increased; a new, broad toeprint that represents additional ribosomes stalled on the uORF appeared 21 to 30 nucleotides upstream of this site; and the toeprint signal corresponding to ribosomes at the downstream initiation codon decreased. These data suggest that arginine increases ribosomal stalling and thereby decreases translation from the downstream initiation codon.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Wang Z,Sachs MSdoi
10.1128/mcb.17.9.4904subject
Has Abstractpub_date
1997-09-01 00:00:00pages
4904-13issue
9eissn
0270-7306issn
1098-5549journal_volume
17pub_type
杂志文章abstract::Nuclear microinjection of c-H-ras DNA induced DNA synthesis in reversibly nonproliferating quiescent human cells. The proto-oncogene and oncogene forms were equally effective inducers. In contrast, c-H-ras DNA either alone or in combination with the adenovirus E1A gene did not cause terminally nondividing senescent ce...
journal_title:Molecular and cellular biology
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doi:10.1128/mcb.6.8.2990
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更新日期:1988-01-01 00:00:00
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doi:10.1128/mcb.18.4.2184
更新日期:1998-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/mcb.4.8.1460
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pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00