Nuclease-deficient FEN-1 blocks Rad51/BRCA1-mediated repair and causes trinucleotide repeat instability.

abstract：:The human splicing factor 2, also called human alternative splicing factor (hASF), is the prototype of the highly conserved SR protein family involved in constitutive and regulated splicing of metazoan mRNA precursors. Here we report that the Drosophila homologue of hASF (dASF) lacks eight repeating arginine-serine di...

journal_title：Molecular and cellular biology

authors： Allemand E,Gattoni R,Bourbon HM,Stevenin J,Cáceres JF,Soret J,Tazi J

更新日期：2001-02-01 00:00:00

abstract：:The degradation of many proteins involves the sequential ligation of ubiquitin molecules to the substrate to form a multiubiquitin chain linked through Lys-48 of ubiquitin. To test for the existence of alternate forms of multiubiquitin chains, we examined the effects of individually substituting each of six other Lys ...

journal_title：Molecular and cellular biology

authors： Spence J,Sadis S,Haas AL,Finley D

更新日期：1995-03-01 00:00:00

abstract：:Nucleolin is a ubiquitous multifunctional protein involved in preribosome assembly and associated with both nucleolar chromatin in interphase and nucleolar organizer regions on metaphasic chromosomes in mitosis. Extensive nucleolin phosphorylation by a casein kinase (CKII) occurs on serine in growing cells. Here we re...

journal_title：Molecular and cellular biology

authors： Belenguer P,Caizergues-Ferrer M,Labbé JC,Dorée M,Amalric F

更新日期：1990-07-01 00:00:00

abstract：:CD8+ T-cell apoptosis is essential for the contraction phase of the immune response, yet the initiating signals and precise pathways involved are unresolved. The ST3Gal-I sialyltransferase is a candidate mechanistic component and catalyzes sialic acid addition to core 1 O-glycans during protein O glycosylation. ST3Gal...

journal_title：Molecular and cellular biology

authors： Van Dyken SJ,Green RS,Marth JD

更新日期：2007-02-01 00:00:00

abstract：:The observation that transcriptionally active genes generally replicate early in S phase and observations of the interaction between transcription factors and replication proteins support the thesis that promoter elements may have a role in DNA replication. To test the relationship between transcription and replicatio...

journal_title：Molecular and cellular biology

authors： Ghosh M,Liu G,Randall G,Bevington J,Leffak M

更新日期：2004-12-01 00:00:00

abstract：:The yeast protein Spa2p localizes to growth sites and is important for polarized morphogenesis during budding, mating, and pseudohyphal growth. To better understand the role of Spa2p in polarized growth, we analyzed regions of the protein important for its function and proteins that interact with Spa2p. Spa2p interact...

journal_title：Molecular and cellular biology

authors： Sheu YJ,Santos B,Fortin N,Costigan C,Snyder M

更新日期：1998-07-01 00:00:00

abstract：:The Reelin signaling cascade plays a crucial role in the correct positioning of neurons during embryonic brain development. Reelin binding to apolipoprotein E receptor 2 (ApoER2) and very-low-density-lipoprotein receptor (VLDLR) leads to phosphorylation of disabled 1 (Dab1), an adaptor protein which associates with th...

journal_title：Molecular and cellular biology

authors： Strasser V,Fasching D,Hauser C,Mayer H,Bock HH,Hiesberger T,Herz J,Weeber EJ,Sweatt JD,Pramatarova A,Howell B,Schneider WJ,Nimpf J

更新日期：2004-02-01 00:00:00

abstract：:We previously established that the phage phiC31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors. We show here that phage attP sites inserted at various locations in human and mouse chromosomes serve...

journal_title：Molecular and cellular biology

authors： Thyagarajan B,Olivares EC,Hollis RP,Ginsburg DS,Calos MP

更新日期：2001-06-01 00:00:00

abstract：:The carcinoembryonic antigen (CEA) family consists of a large group of evolutionarily and structurally divergent glycoproteins. The murine CEACAM9 and CEACAM11-related proteins as well as the pregnancy-specific glycoproteins (PSG) are secreted members of the CEA family which are differentially expressed in fetal troph...

journal_title：Molecular and cellular biology

authors： Finkenzeller D,Fischer B,Lutz S,Schrewe H,Shimizu T,Zimmermann W

更新日期：2003-01-01 00:00:00

abstract：:Changes in chromatin composition are often a prerequisite for gene induction. Nonallelic histone variants have recently emerged as key players in transcriptional control and chromatin modulation. While the changes in chromatin accessibility and histone posttranslational modification (PTM) distribution that accompany g...

journal_title：Molecular and cellular biology

authors： Sutcliffe EL,Parish IA,He YQ,Juelich T,Tierney ML,Rangasamy D,Milburn PJ,Parish CR,Tremethick DJ,Rao S

更新日期：2009-04-01 00:00:00

abstract：:Cells of Candida albicans WO-1 spontaneously switch between a white and opaque CFU, and this phase transition involves a dramatic change in cellular phenotype. By using a differential hybridization screen, an opaque-specific cDNA, Op1a, which represents the transcript of a gene regulated by switching, has been isolate...

journal_title：Molecular and cellular biology

authors： Morrow B,Srikantha T,Soll DR

更新日期：1992-07-01 00:00:00

abstract：:An in silico screen for myogenic long noncoding RNAs (lncRNAs) revealed nine lncRNAs that are upregulated more than 10-fold in myotubes versus levels in myoblasts. One of these lncRNAs, MyoD upstream noncoding (MUNC, also known as DRR(eRNA)), is encoded 5 kb upstream of the transcription start site of MyoD, a myogenic...

journal_title：Molecular and cellular biology

authors： Mueller AC,Cichewicz MA,Dey BK,Layer R,Reon BJ,Gagan JR,Dutta A

更新日期：2015-02-01 00:00:00

abstract：:Two-dimensional protein gels were used to systematically assess changes in gene expression in Friend erythroleukemia cells after exposure to inducers of differentiation. A rapid decrease in expression of the stress protein HSP70 was observed after exposure to inducers. The kinetics of this change suggest that it may b...

journal_title：Molecular and cellular biology

authors： Hensold JO,Housman DE

更新日期：1988-05-01 00:00:00

abstract：:Cyclooxygenase (COX) catalyzes the first step in prostanoid biosynthesis and exists as two isoforms. COX-1 is a constitutive enzyme involved in physiological processes, whereas COX-2 is induced by a variety of stimuli. MicroRNAs (miRNAs) are noncoding RNAs that function as key posttranscriptional regulators of gene ex...

journal_title：Molecular and cellular biology

authors： Motiño O,Francés DE,Mayoral R,Castro-Sánchez L,Fernández-Velasco M,Boscá L,García-Monzón C,Brea R,Casado M,Agra N,Martín-Sanz P

更新日期：2015-07-01 00:00:00

abstract：:ADAR2 is a double-stranded-RNA-specific adenosine deaminase involved in the editing of mammalian RNAs by the site-selective conversion of adenosine to inosine. Previous studies from our laboratory have demonstrated that ADAR2 can modify its own pre-mRNA to create a proximal 3' splice site containing a noncanonical ade...

journal_title：Molecular and cellular biology

authors： Feng Y,Sansam CL,Singh M,Emeson RB

更新日期：2006-01-01 00:00:00

abstract：:S-Tubercidinylhomocysteine (STH) is a structural analog of S-adenosylhomocysteine and a potent inhibitor of S-adenosylmethionine-dependent methyltransferase reactions. We investigated the effects of STH on HeLa cell mRNA metabolism. Dual labeling studies reveal that STH dramatically inhibits the methylation of HeLa mR...

journal_title：Molecular and cellular biology

authors： Camper SA,Albers RJ,Coward JK,Rottman FM

更新日期：1984-03-01 00:00:00

abstract：:It is well established that cell survival signals stimulated by growth factors, cytokines, and oncoproteins are initiated by phosphoinositide 3-kinase (PI3K)- and Akt-dependent signal transduction pathways. Oncogenic Ras, an upstream activator of Akt, requires NF-kappaB to initiate transformation, at least partially t...

journal_title：Molecular and cellular biology

authors： Madrid LV,Wang CY,Guttridge DC,Schottelius AJ,Baldwin AS Jr,Mayo MW

更新日期：2000-03-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a potent angiogenic inducer that stimulates the expression of tissue factor (TF), the major cellular initiator of blood coagulation. Here we show that signaling triggered by VEGF induced DNA-binding and transcriptional activities of nuclear factor of activated T cells (NFAT...

journal_title：Molecular and cellular biology

authors： Armesilla AL,Lorenzo E,Gómez del Arco P,Martínez-Martínez S,Alfranca A,Redondo JM

更新日期：1999-03-01 00:00:00

abstract：:Fibroblast growth factors (FGFs) induce a variety of biological effects on different cell types. They activate a number of genes, including immediate-early genes, such as the transcription factors Fos and Jun, which are also common targets for other tyrosine kinase receptor-activating growth factors. Here we describe ...

journal_title：Molecular and cellular biology

authors： Jaakkola P,Vihinen T,Määttä A,Jalkanen M

更新日期：1997-06-01 00:00:00

abstract：:SH2 (src homology region 2) domains are implicated in protein-protein interactions involved in signal transduction pathways. Isolated SH2 domains bind proteins that are tyrosine phosphorylated. A novel, phosphotyrosine-independent binding interaction between BCR, the Philadelphia chromosome breakpoint cluster region g...

journal_title：Molecular and cellular biology

authors： Muller AJ,Pendergast AM,Havlik MH,Puil L,Pawson T,Witte ON

更新日期：1992-11-01 00:00:00

abstract：:The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at th...

journal_title：Molecular and cellular biology

authors： Hsu JM,Huang J,Meluh PB,Laurent BC

更新日期：2003-05-01 00:00:00

abstract：:Although largely involved in innate and adaptive immunity, NF-kappa B plays an important role in vertebrate development. In chicks, the inactivation of the NF-kappa B pathway induces functional alterations of the apical ectodermal ridge, which mediates limb outgrowth. In mice, the complete absence of NF-kappa B activi...

journal_title：Molecular and cellular biology

authors： Correa RG,Tergaonkar V,Ng JK,Dubova I,Izpisua-Belmonte JC,Verma IM

更新日期：2004-06-01 00:00:00

abstract：:Using competition mobility shift, methylation interference, and proteolytic clipping DNA binding assays, we demonstrate that the protein binding the major histocompatibility complex A beta CCAAT box is indistinguishable from the protein previously named NF-Y, which binds the major histocompatibility complex E alpha CC...

journal_title：Molecular and cellular biology

authors： Kern MJ,Woodward JG

更新日期：1991-01-01 00:00:00

abstract：:Minichromosome maintenance protein 1 (Mcm1) is required for efficient replication of autonomously replicating sequence (ARS)-containing plasmids in yeast cells. Reduced DNA binding activity in the Mcm1-1 mutant protein (P97L) results in selective initiation of a subset of replication origins and causes instability of ...

journal_title：Molecular and cellular biology

authors： Chang VK,Donato JJ,Chan CS,Tye BK

更新日期：2004-07-01 00:00:00

abstract：:TIN2 is a negative regulator of telomere elongation that interacts with telomeric DNA repeat binding factor 1 (TRF1) and affects telomere length by a telomerase-dependent mechanism. Here we show that inactivation of the mouse TRF1-interacting protein 2 (TIN2) gene results in early embryonic lethality. We further obser...

journal_title：Molecular and cellular biology

authors： Chiang YJ,Kim SH,Tessarollo L,Campisi J,Hodes RJ

更新日期：2004-08-01 00:00:00

abstract：:DNA from the centromere region of linkage group (LG) VII of Neurospora crassa was cloned previously from a yeast artificial chromosome library and was found to be atypical of Neurospora DNA in both composition (AT rich) and complexity (repetitive). We have determined the DNA sequence of a small portion (approximately ...

journal_title：Molecular and cellular biology

authors： Cambareri EB,Aisner R,Carbon J

更新日期：1998-09-01 00:00:00

abstract：:The regulation of telomerase reverse transcriptase (TERT) plays an important role in the proliferative capacity and survival of cells. Here, we report that exogenously as well as endogenously induced oxidative stress leads to translocation of endogenous as well as overexpressed human TERT from the nucleus into the cyt...

journal_title：Molecular and cellular biology

authors： Haendeler J,Hoffmann J,Brandes RP,Zeiher AM,Dimmeler S

更新日期：2003-07-01 00:00:00

abstract：:Insulin stimulates glucose transport into fat and muscle cells by increasing the exocytic trafficking rate of the GLUT4 facilitative glucose transporter from intracellular stores to the plasma membrane. Delivery of GLUT4 to the plasma membrane is mediated by formation of functional SNARE complexes containing syntaxin4...

journal_title：Molecular and cellular biology

authors： Kioumourtzoglou D,Gould GW,Bryant NJ

更新日期：2014-04-01 00:00:00

abstract：:The hexanucleotide AAUAAA has been demonstrated to be part of the signal for cleavage and polyadenylation at appropriate sites on eucaryotic mRNA precursors. Since this sequence is not unique to polyadenylation sites, it cannot be the entire signal for the cleavage event. We have extended the definition of the polyade...

journal_title：Molecular and cellular biology

authors： Sadofsky M,Alwine JC

更新日期：1984-08-01 00:00:00

abstract：:A transcriptional enhancer has been mapped to a region 5.5 kilobases 3' of the C beta 2 gene in the human T-cell receptor (TCR) beta-chain locus. Transient transfections allowed localization of enhancer activity to a 480-base-pair HincII-XbaI restriction enzyme fragment. The TCR beta enhancer was active on both the mi...

journal_title：Molecular and cellular biology

authors： Gottschalk LR,Leiden JM

更新日期：1990-10-01 00:00:00