Abstract:
:We previously established that the phage phiC31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors. We show here that phage attP sites inserted at various locations in human and mouse chromosomes serve as efficient targets for precise site-specific integration. Moreover, we characterize native "pseudo" attP sites in the human and mouse genomes that also mediate efficient integrase-mediated integration. These sites have partial sequence identity to attP. Such sites form naturally occurring targets for integration. This phage integrase-mediated reaction represents an effective site-specific integration system for higher cells and may be of value in gene therapy and other chromosome engineering strategies.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Thyagarajan B,Olivares EC,Hollis RP,Ginsburg DS,Calos MPdoi
10.1128/MCB.21.12.3926-3934.2001subject
Has Abstractpub_date
2001-06-01 00:00:00pages
3926-34issue
12eissn
0270-7306issn
1098-5549journal_volume
21pub_type
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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journal_title:Molecular and cellular biology
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2001-10-01 00:00:00
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doi:10.1128/mcb.23.19.6798-6808.2003
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.23.24.9303-9317.2003
更新日期:2003-12-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.13.7.4157
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pub_type: 杂志文章
doi:10.1128/mcb.12.4.1674
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00