The protein encoded by a growth arrest-specific gene (gas6) is a new member of the vitamin K-dependent proteins related to protein S, a negative coregulator in the blood coagulation cascade.

Abstract:

:A set of growth arrest-specific genes (gas) whose expression is negatively regulated after serum induction has previously been described (C. Schneider, R. M. King, and L. Philipson, Cell 54:787-793, 1988). The detailed analysis of one of them, gas6, is reported here, gas6 mRNA (2.6 kb) is abundantly expressed in serum-starved (48 h in 0.5% fetal calf serum) NIH 3T3 cells but decreases dramatically after fetal calf serum or basic fibroblast growth factor stimulation. The human homolog of gas6 was also cloned and sequenced, revealing a high degree of homology and a similar pattern of expression in IMR90 human fibroblasts. Computer analysis of the protein encoded by murine and human gas6 cDNAs showed significant homology (43 and 44% amino acid identity, respectively) to human protein S, a negative coregulator in the blood coagulation pathway. By using an anti-human Gas6 monospecific affinity-purified antibody, we show that the biosynthetic level of human Gas6 fully reflects mRNA expression in IMR90 human fibroblasts. This finding thus defines a new member of vitamin K-dependent proteins that is expressed in many human and mouse tissues and may be involved in the regulation of a protease cascade relevant in growth regulation.

journal_name

Mol Cell Biol

authors

Manfioletti G,Brancolini C,Avanzi G,Schneider C

doi

10.1128/mcb.13.8.4976

subject

Has Abstract

pub_date

1993-08-01 00:00:00

pages

4976-85

issue

8

eissn

0270-7306

issn

1098-5549

journal_volume

13

pub_type

杂志文章
  • Fibronectin is overproduced by keloid fibroblasts during abnormal wound healing.

    abstract::Wound healing in certain individuals leads to the development of keloid tumors which exhibit abnormal collagen metabolism and an increased abundance of extracellular matrix components. Comparison of fibronectin levels in fibroblasts derived from keloids and normal dermis revealed a relative increase in intracellular a...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.9.4.1642

    authors: Babu M,Diegelmann R,Oliver N

    更新日期:1989-04-01 00:00:00

  • Ikaros and GATA-1 combinatorial effect is required for silencing of human gamma-globin genes.

    abstract::During development and erythropoiesis, globin gene expression is finely modulated through an important network of transcription factors and chromatin modifying activities. In this report we provide in vivo evidence that endogenous Ikaros is recruited to the human beta-globin locus and targets the histone deacetylase H...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01523-08

    authors: Bottardi S,Ross J,Bourgoin V,Fotouhi-Ardakani N,Affar el B,Trudel M,Milot E

    更新日期:2009-03-01 00:00:00

  • Drosophila DBT lacking protein kinase activity produces long-period and arrhythmic circadian behavioral and molecular rhythms.

    abstract::A mutation (K38R) which specifically eliminates kinase activity was created in the Drosophila melanogaster ckI gene (doubletime [dbt]). In vitro, DBT protein carrying the K38R mutation (DBT(K/R)) interacted with Period protein (PER) but lacked kinase activity. In cell culture and in flies, DBT(K/R) antagonized the pho...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00680-07

    authors: Muskus MJ,Preuss F,Fan JY,Bjes ES,Price JL

    更新日期:2007-12-01 00:00:00

  • An electrophoretic karyotype of Neurospora crassa.

    abstract::A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated s...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.8.4.1469

    authors: Orbach MJ,Vollrath D,Davis RW,Yanofsky C

    更新日期:1988-04-01 00:00:00

  • Association and regulation of heat shock transcription factor 4b with both extracellular signal-regulated kinase mitogen-activated protein kinase and dual-specificity tyrosine phosphatase DUSP26.

    abstract::The heat shock transcription factors (Hsfs) activate the stress-inducible expression of heat shock proteins (Hsps) and other molecular chaperones in response to stress and, therefore, play an essential role in protein disaggregation and protein folding. In humans, missense mutation in the hsf4 gene causes cataract, an...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.26.8.3282-3294.2006

    authors: Hu Y,Mivechi NF

    更新日期:2006-04-01 00:00:00

  • Evidence that protein binding specifies sites of DNA demethylation.

    abstract::It has been hypothesized that protein factors may protect CpG islands from methyltransferase during development and that demethylation may involve protein-DNA interactions at demethylated sites. However, direct evidence has been lacking. In this study, demethylation at the EBNA-1 binding sites of the Epstein-Barr viru...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.19.1.46

    authors: Hsieh CL

    更新日期:1999-01-01 00:00:00

  • Hydrogen peroxide triggers nuclear export of telomerase reverse transcriptase via Src kinase family-dependent phosphorylation of tyrosine 707.

    abstract::The regulation of telomerase reverse transcriptase (TERT) plays an important role in the proliferative capacity and survival of cells. Here, we report that exogenously as well as endogenously induced oxidative stress leads to translocation of endogenous as well as overexpressed human TERT from the nucleus into the cyt...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.23.13.4598-4610.2003

    authors: Haendeler J,Hoffmann J,Brandes RP,Zeiher AM,Dimmeler S

    更新日期:2003-07-01 00:00:00

  • The transcriptional coactivator Cbp regulates self-renewal and differentiation in adult hematopoietic stem cells.

    abstract::The transcriptional coactivator Cbp plays an important role in a wide range of cellular processes, including proliferation, differentiation, and apoptosis. Although studies have shown its requirement for hematopoietic stem cell (HSC) development, its role in adult HSC maintenance, as well as the cellular and molecular...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.05830-11

    authors: Chan WI,Hannah RL,Dawson MA,Pridans C,Foster D,Joshi A,Göttgens B,Van Deursen JM,Huntly BJ

    更新日期:2011-12-01 00:00:00

  • p53 mediates senescence-like arrest induced by chronic replicational stress.

    abstract::Previous studies have shown that exposure of cells to high levels of replicational stress leads to permanent proliferation arrest that does not require p53. We have examined cellular responses to therapeutically relevant low levels of replicational stress that allow limited proliferation. Chronic exposure to low conce...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01316-06

    authors: Marusyk A,Wheeler LJ,Mathews CK,DeGregori J

    更新日期:2007-08-01 00:00:00

  • Cooperative action of multiple cis-acting elements is required for N-myc expression in branchial arches: specific contribution of GATA3.

    abstract::The precise expression of the N-myc proto-oncogene is essential for normal mammalian development, whereas altered N-myc gene regulation is known to be a determinant factor in tumor formation. Using transgenic mouse embryos, we show that N-myc sequences from kb -8.7 to kb +7.2 are sufficient to reproduce the N-myc embr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00353-09

    authors: Potvin E,Beuret L,Cadrin-Girard JF,Carter M,Roy S,Tremblay M,Charron J

    更新日期:2010-11-01 00:00:00

  • Signaling in the yeast pheromone response pathway: specific and high-affinity interaction of the mitogen-activated protein (MAP) kinases Kss1 and Fus3 with the upstream MAP kinase kinase Ste7.

    abstract::Kss1 and Fus3 are mitogen-activated protein kinases (MAPKs or ERKs), and Ste7 is their activating MAPK/ERK kinase (MEK), in the pheromone response pathway of Saccharomyces cerevisiae. To investigate the potential role of specific interactions between these enzymes during signaling, their ability to associate with each...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.16.7.3637

    authors: Bardwell L,Cook JG,Chang EC,Cairns BR,Thorner J

    更新日期:1996-07-01 00:00:00

  • Integration of estrogen and Wnt signaling circuits by the polycomb group protein EZH2 in breast cancer cells.

    abstract::Essential for embryonic development, the polycomb group protein enhancer of zeste homolog 2 (EZH2) is overexpressed in breast and prostate cancers and is implicated in the growth and aggression of the tumors. The tumorigenic mechanism underlying EZH2 overexpression is largely unknown. It is believed that EZH2 exerts i...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00162-07

    authors: Shi B,Liang J,Yang X,Wang Y,Zhao Y,Wu H,Sun L,Zhang Y,Chen Y,Li R,Zhang Y,Hong M,Shang Y

    更新日期:2007-07-01 00:00:00

  • Transcriptional properties of BmX, a moderately repetitive silkworm gene that is an RNA polymerase III template.

    abstract::We analyzed the transcriptional properties of a repetitive sequence element, BmX, that belongs to a large gene family (approximately 2 x 10(4) copies) in the genome of the Bombyx mori silkworm. We discovered BmX elements because of their ability to direct transcription by polymerase III in vitro and used them to test ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.8.2.624

    authors: Wilson ET,Condliffe DP,Sprague KU

    更新日期:1988-02-01 00:00:00

  • A conserved docking motif for CK1 binding controls the nuclear localization of NFAT1.

    abstract::In resting cells, the NFAT1 transcription factor is kept inactive in the cytoplasm by phosphorylation on multiple serine residues. These phosphorylated residues are primarily contained within two types of serine-rich motifs, the SRR-1 and SP motifs, which are conserved within the NFAT family. Several different kinases...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.24.10.4184-4195.2004

    authors: Okamura H,Garcia-Rodriguez C,Martinson H,Qin J,Virshup DM,Rao A

    更新日期:2004-05-01 00:00:00

  • Influence of the RNA-binding protein HuR in pVHL-regulated p53 expression in renal carcinoma cells.

    abstract::A recent analysis of gene expression in renal cell carcinoma cells led to the identification of mRNAs whose translation was dependent on the presence of the von Hippel-Lindau (VHL) tumor suppressor gene product, pVHL. Here, we investigate the finding that pVHL-expressing RCC cells (VHL(+)) exhibited elevated levels of...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.23.20.7083-7095.2003

    authors: Galbán S,Martindale JL,Mazan-Mamczarz K,López de Silanes I,Fan J,Wang W,Decker J,Gorospe M

    更新日期:2003-10-01 00:00:00

  • Conditional deletion of focal adhesion kinase leads to defects in ventricular septation and outflow tract alignment.

    abstract::To examine a role for focal adhesion kinase (FAK) in cardiac morphogenesis, we generated a line of mice with a conditional deletion of FAK in nkx2-5-expressing cells (herein termed FAKnk mice). FAKnk mice died shortly after birth, likely resulting from a profound subaortic ventricular septal defect and associated mala...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00068-07

    authors: Hakim ZS,DiMichele LA,Doherty JT,Homeister JW,Beggs HE,Reichardt LF,Schwartz RJ,Brackhan J,Smithies O,Mack CP,Taylor JM

    更新日期:2007-08-01 00:00:00

  • The first pre-rRNA-processing event occurs in a large complex: analysis by gel retardation, sedimentation, and UV cross-linking.

    abstract::The first processing event that mouse pre-rRNA undergoes occurs within the external transcribed spacer and is efficiently reproduced in vitro. Analysis with nondenaturing polyacrylamide gels revealed the formation of heparin-resistant complexes of retarded electrophoretic mobility on the substrate rRNA. The specificit...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.10.9.4920

    authors: Kass S,Sollner-Webb B

    更新日期:1990-09-01 00:00:00

  • CRM1 mediates the export of ADAR1 through a nuclear export signal within the Z-DNA binding domain.

    abstract::RNA editing of specific residues by adenosine deamination is a nuclear process catalyzed by adenosine deaminases acting on RNA (ADAR). Different promoters in the ADAR1 gene give rise to two forms of the protein: a constitutive promoter expresses a transcript encoding (c)ADAR1, and an interferon-induced promoter expres...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.21.22.7862-7871.2001

    authors: Poulsen H,Nilsson J,Damgaard CK,Egebjerg J,Kjems J

    更新日期:2001-11-01 00:00:00

  • Transcription factor Nkx-2.5 induces sodium/iodide symporter gene expression and participates in retinoic acid- and lactation-induced transcription in mammary cells.

    abstract::The sodium/iodide symporter (NIS) is a plasma membrane protein that mediates active iodide transport in thyroid and mammary cells. It is a prerequisite for radioiodide treatment of thyroid cancer and a promising diagnostic and therapeutic tool for breast cancer. We investigated the molecular mechanisms governing NIS e...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.24.18.7863-7877.2004

    authors: Dentice M,Luongo C,Elefante A,Romino R,Ambrosio R,Vitale M,Rossi G,Fenzi G,Salvatore D

    更新日期:2004-09-01 00:00:00

  • In vivo dynamics of Swi6 in yeast: evidence for a stochastic model of heterochromatin.

    abstract::The mechanism for transcriptional silencing of pericentric heterochromatin is conserved from fission yeast to mammals. Silenced genome regions are marked by epigenetic methylation of histone H3, which serves as a binding site for structural heterochromatin proteins. In the fission yeast Schizosaccharomyces pombe, the ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.24.8.3157-3167.2004

    authors: Cheutin T,Gorski SA,May KM,Singh PB,Misteli T

    更新日期:2004-04-01 00:00:00

  • Identification of positive-acting domains in GCN2 protein kinase required for translational activation of GCN4 expression.

    abstract::GCN4 is a transcriptional activator of amino acid-biosynthetic genes in the yeast Saccharomyces cerevisiae. GCN2, a translational activator of GCN4 expression, contains a domain homologous to the catalytic subunit of eucaryotic protein kinases. Substitution of a highly conserved lysine residue in the kinase domain abo...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.10.6.2820

    authors: Wek RC,Ramirez M,Jackson BM,Hinnebusch AG

    更新日期:1990-06-01 00:00:00

  • Nuclear pre-mRNA decapping and 5' degradation in yeast require the Lsm2-8p complex.

    abstract::Previous analyses have identified related cytoplasmic Lsm1-7p and nuclear Lsm2-8p complexes. Here we report that mature heat shock and MET mRNAs that are trapped in the nucleus due to a block in mRNA export were strongly stabilized in strains lacking Lsm6p or the nucleus-specific Lsm8p protein but not by the absence o...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.24.21.9646-9657.2004

    authors: Kufel J,Bousquet-Antonelli C,Beggs JD,Tollervey D

    更新日期:2004-11-01 00:00:00

  • The second catalytic domain of protein tyrosine phosphatase delta (PTP delta) binds to and inhibits the first catalytic domain of PTP sigma.

    abstract::The LAR family protein tyrosine phosphatases (PTPs), including LAR, PTP delta, and PTP sigma, are transmembrane proteins composed of a cell adhesion molecule-like ectodomain and two cytoplasmic catalytic domains: active D1 and inactive D2. We performed a yeast two-hybrid screen with the first catalytic domain of PTP s...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.18.5.2608

    authors: Wallace MJ,Fladd C,Batt J,Rotin D

    更新日期:1998-05-01 00:00:00

  • 2-Aminopurine selectively inhibits splicing of tumor necrosis factor alpha mRNA.

    abstract::2-Aminopurine (2-AP) inhibits specific kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2. One of these, PKR, is also involved in signal transduction. We show here that 2-AP selectively inhibits expression of tumor necrosis factor alpha (TNF-alpha) mRNA in primary human lymphoid...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.16.6.2814

    authors: Jarrous N,Osman F,Kaempfer R

    更新日期:1996-06-01 00:00:00

  • Lysine Acetylation of CREBH Regulates Fasting-Induced Hepatic Lipid Metabolism.

    abstract::Cyclic AMP-responsive element-binding protein 3-like 3, hepatocyte specific (CREBH), is a hepatic transcription factor that functions as a key regulator of energy homeostasis. Here, we defined a regulatory CREBH posttranslational modification process, namely, lysine-specific acetylation, and its functional involvement...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00665-15

    authors: Kim H,Mendez R,Chen X,Fang D,Zhang K

    更新日期:2015-12-01 00:00:00

  • Novel role for cyclophilin A in regulation of chondrogenic commitment and endochondral ossification.

    abstract::Recent studies showed that cyclophilin A (CypA) promotes NF-κB/p65 nuclear translocation, resulting in enhanced NF-κB activity and altered expression of its target genes, such as the Sox9 transcriptional factor, which plays a critical role in chondrogenic differentiation and endochondral ossification. In this report, ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01414-14

    authors: Guo M,Shen J,Kwak JH,Choi B,Lee M,Hu S,Zhang X,Ting K,Soo CB,Chiu RH

    更新日期:2015-06-01 00:00:00

  • Roles of fetal G gamma-globin promoter elements and the adult beta-globin 3' enhancer in the stage-specific expression of globin genes.

    abstract::The human fetal G gamma-globin and adult beta-globin genes are expressed in a tissue- and developmental stage-specific pattern in transgenic mice: the G gamma gene in embryonic cells and the beta gene in fetal and adult erythroid cells. Several of the cis-acting DNA sequences thought to be responsible for these patter...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.10.3.1116

    authors: Perez-Stable C,Costantini F

    更新日期:1990-03-01 00:00:00

  • Adenovirus origin of DNA replication: sequence requirements for replication in vitro.

    abstract::The initiation of adenovirus DNA takes place at the termini of the viral genome and requires the presence of specific nucleotide sequence elements. To define the sequence organization of the viral origin, we tested a large number of deletion, insertion, and base substitution mutants for their ability to support initia...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.7.2.864

    authors: Wides RJ,Challberg MD,Rawlins DR,Kelly TJ

    更新日期:1987-02-01 00:00:00

  • Estrogen receptors recruit SMRT and N-CoR corepressors through newly recognized contacts between the corepressor N terminus and the receptor DNA binding domain.

    abstract::Estrogen receptors (ERs) are hormone-regulated transcription factors that regulate key aspects of reproduction and development. ERs are unusual in that they do not typically repress transcription in the absence of hormone but instead possess otherwise cryptic repressive functions that are revealed upon binding to cert...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01002-09

    authors: Varlakhanova N,Snyder C,Jose S,Hahm JB,Privalsky ML

    更新日期:2010-03-01 00:00:00

  • Genetic interaction between transcription elongation factor TFIIS and RNA polymerase II.

    abstract::Little is known about the regions of RNA polymerase II (RNAPII) that are involved in the process of transcript elongation and interaction with elongation factors. One elongation factor, TFIIS, stimulates transcript elongation by binding to RNAPII and facilitating its passage through intrinsic pausing sites in vitro. I...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.12.9.4142

    authors: Archambault J,Lacroute F,Ruet A,Friesen JD

    更新日期:1992-09-01 00:00:00