The SET2-RPB1 interaction domain of human RECQ5 is important for transcription-associated genome stability.

abstract：:Enamel mineralization is accompanied by the release of protons into the extracellular matrix, which is buffered to regulate the pH value in the local microenvironment. The present study aimed to investigate the role of microRNA 224 (miR-224) as a regulator of SLC4A4 and CFTR, encoding the key buffering ion transporter...

journal_title：Molecular and cellular biology

authors： Fan Y,Zhou Y,Zhou X,Sun F,Gao B,Wan M,Zhou X,Sun J,Xu X,Cheng L,Crane J,Zheng L

更新日期：2015-08-01 00:00:00

abstract：:The c-fes locus encodes a 93-kDa non-receptor protein tyrosine kinase (Fes) that regulates the growth and differentiation of hematopoietic and vascular endothelial cells. Unique to Fes is a long N-terminal sequence with two regions of strong homology to coiled-coil oligomerization domains. We introduced leucine-to-pro...

journal_title：Molecular and cellular biology

authors： Cheng HY,Schiavone AP,Smithgall TE

更新日期：2001-09-01 00:00:00

abstract：:Repair of chromosomal breaks is essential for cellular viability, but misrepair generates mutations and gross chromosomal rearrangements. We investigated the interrelationship between two homologous-repair pathways, i.e., mutagenic single-strand annealing (SSA) and precise homology-directed repair (HDR). For this, we ...

journal_title：Molecular and cellular biology

authors： Stark JM,Pierce AJ,Oh J,Pastink A,Jasin M

更新日期：2004-11-01 00:00:00

abstract：:It is well established that steroid receptor function requires interaction with coactivators. However, the mechanisms through which steroid receptors elicit precise assembly of coactivator complexes and the way the steroid activation signal is transduced remain elusive. Using a T47D cell line stably integrated with a ...

journal_title：Molecular and cellular biology

authors： Li X,Wong J,Tsai SY,Tsai MJ,O'Malley BW

更新日期：2003-06-01 00:00:00

abstract：:In mast cells, antigen-mediated aggregation of the high-affinity receptor for immunoglobulin E, Fc epsilon RI, stimulates tyrosine phosphorylation and activation of multiple signaling pathways leading to the release of several classes of mediators of the allergic response. Early events induced upon cross-linking of Fc...

journal_title：Molecular and cellular biology

authors： Rivera VM,Brugge JS

更新日期：1995-03-01 00:00:00

abstract：:The single-copy gene encoding the alpha subunit of glycoprotein hormones is expressed in the pituitaries of all mammals and in the placentas of only primates and horses. We have systematically analyzed the promoter-regulatory elements of the human and bovine alpha-subunit genes to elucidate the molecular mechanisms un...

journal_title：Molecular and cellular biology

authors： Bokar JA,Keri RA,Farmerie TA,Fenstermaker RA,Andersen B,Hamernik DL,Yun J,Wagner T,Nilson JH

更新日期：1989-11-01 00:00:00

abstract：:Nucleolar morphogenesis is a poorly defined process. Here we report that the Saccharomyces cerevisiae nucleolar trimethyl guanosine synthase I (Tgs1p), which specifically selects the m(7)G cap structure of snRNAs and snoRNAs for m(2,2,7)G conversion, is required not only for efficient pre-mRNA splicing but also for pr...

journal_title：Molecular and cellular biology

authors： Colau G,Thiry M,Leduc V,Bordonné R,Lafontaine DL

更新日期：2004-09-01 00:00:00

abstract：:Tumor necrosis factor alpha (TNF alpha) is a key regulatory cytokine whose expression is controlled by a complex set of stimuli in a variety of cell types. Previously, we found that the monocyte/macrophage-enriched nuclear transcription factor C/EBPbeta played an important role in the regulation of the TNF alpha gene ...

journal_title：Molecular and cellular biology

authors： Zagariya A,Mungre S,Lovis R,Birrer M,Ness S,Thimmapaya B,Pope R

更新日期：1998-05-01 00:00:00

abstract：:The simian virus 40 large T antigen (T antigen) inactivates tumor suppressor proteins and therefore has been used in numerous studies to probe the mechanisms that control cellular growth and to generate immortalized cell lines. Binding of T antigen to the Rb family of growth-regulatory proteins is necessary but not su...

journal_title：Molecular and cellular biology

authors： Sullivan CS,Cantalupo P,Pipas JM

更新日期：2000-09-01 00:00:00

abstract：:A set of genes is rapidly inducible when quiescent fibroblasts are stimulated by growth factors or by the activation of temperature-sensitive retroviral protein-tyrosine kinases. Most of these so-called immediate-early genes were cloned by differential cDNA hybridization. DNA sequence analysis identified many of them ...

journal_title：Molecular and cellular biology

authors： Jähner D,Hunter T

更新日期：1991-07-01 00:00:00

abstract：:Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks (DSBs) catalyzed by the Spo11 protein. DSBs are not randomly distributed along chromosomes. To better understand factors that control the distribution of DSBs in budding yeast, we have examined the genome-wide binding and cleava...

journal_title：Molecular and cellular biology

authors： Robine N,Uematsu N,Amiot F,Gidrol X,Barillot E,Nicolas A,Borde V

更新日期：2007-03-01 00:00:00

abstract：:This paper presents a general means of eliminating the function of a single protein without relying on genetic alterations in its structure or level of synthesis. The strategy is based on the inducible cellular expression of neutralizing antibody to inactivate the protein selectively. The feasibility of this approach ...

journal_title：Molecular and cellular biology

authors： Carlson JR

更新日期：1988-06-01 00:00:00

abstract：:The SMRT corepressor complex participates in transcriptional repression by a diverse array of vertebrate transcription factors. The ability to recruit SMRT appears to play a crucial role in leukemogenesis by the PML-retinoic acid receptor alpha (RARalpha) oncoprotein, an aberrant nuclear hormone receptor implicated in...

journal_title：Molecular and cellular biology

authors： Hong SH,Yang Z,Privalsky ML

更新日期：2001-11-01 00:00:00

abstract：:The first intron of the human collagen alpha 1(I) gene contains several positively and negatively acting elements. We have studied the transcription of collagen-human growth hormone fusion genes, containing deletions and rearrangements of collagen intronic sequences, by transient transfection of chick tendon fibroblas...

journal_title：Molecular and cellular biology

authors： Bornstein P,McKay J,Liska DJ,Apone S,Devarayalu S

更新日期：1988-11-01 00:00:00

abstract：:PC12 cells treated with nerve growth factor (NGF) or infected with Rous sarcoma virus differentiate into sympathetic, neuronlike cells. To compare the differentiation programs induced by NGF and v-src, we have established a PC12 cell line expressing a temperature-sensitive v-src protein. The v-src-expressing PC12 cell...

journal_title：Molecular and cellular biology

authors： Thomas SM,Hayes M,D'Arcangelo G,Armstrong RC,Meyer BE,Zilberstein A,Brugge JS,Halegoua S

更新日期：1991-09-01 00:00:00

abstract：:The MyoD gene can orchestrate the expression of the skeletal muscle differentiation program. We have identified the regions of the gene necessary to reproduce transcription specific to skeletal myoblasts and myotubes. A proximal regulatory region (PRR) contains a conserved TATA box, a CCAAT box, and a GC-rich region t...

journal_title：Molecular and cellular biology

authors： Tapscott SJ,Lassar AB,Weintraub H

更新日期：1992-11-01 00:00:00

abstract：:Receptor tyrosine kinases (RTKs) play distinct roles in multiple biological systems. Many RTKs transmit similar signals, raising questions about how specificity is achieved. One potential mechanism for RTK specificity is control of the magnitude and kinetics of activation of downstream pathways. We have found that the...

journal_title：Molecular and cellular biology

authors： Zhang SQ,Tsiaras WG,Araki T,Wen G,Minichiello L,Klein R,Neel BG

更新日期：2002-06-01 00:00:00

abstract：:In many types of apoptosis, the proapoptotic protein Bax undergoes a change in conformation at the level of the mitochondria. This event always precedes the release of mitochondrial cytochrome c, which, in the cytosol, activates caspases through binding to Apaf-1. The mechanisms by which Bax triggers cytochrome c rele...

journal_title：Molecular and cellular biology

authors： Eskes R,Desagher S,Antonsson B,Martinou JC

更新日期：2000-02-01 00:00:00

abstract：:The C proteins (C1 and C2) of HeLa 40S heterogeneous nuclear ribonucleoprotein particles copurify under native conditions as a stable complex with a fixed molar protein ratio (S.F. Barnett, W.M. LeStourgeon, and D.L. Friedman, J. Biochem. Biophys. Methods 16:87-97, 1988). Gel filtration chromatography and velocity sed...

journal_title：Molecular and cellular biology

authors： Barnett SF,Friedman DL,LeStourgeon WM

更新日期：1989-02-01 00:00:00

abstract：:The enhancer of yellow 1 gene, e(y)1, of Drosophila melanogaster has been cloned and demonstrated to encode the TAFII40 protein. The e(y)1 gene is expressed in females much more strongly than in males due to the accumulation of e(y)1 mRNA in the ovaries. Two different e(y)1 mutations have been obtained. The e(y)1(ul) ...

journal_title：Molecular and cellular biology

authors： Soldatov A,Nabirochkina E,Georgieva S,Belenkaja T,Georgiev P

更新日期：1999-05-01 00:00:00

abstract：:We have previously shown by affinity chromatography that RAP30 and RAP74 are the mammalian proteins that have the highest affinity for RNA polymerase II. Here we show that RAP30 binds to RAP74 and that the RAP30-RAP74 complex (RAP30/74) is required for accurate initiation by RNA polymerase II. RAP30/74 is required for...

journal_title：Molecular and cellular biology

authors： Burton ZF,Killeen M,Sopta M,Ortolan LG,Greenblatt J

更新日期：1988-04-01 00:00:00

abstract：:LEU3 of Saccharomyces cerevisiae encodes an 886-amino-acid polypeptide that regulates transcription of a group of genes involved in leucine biosynthesis and has been shown to bind specifically to a 114-base-pair DNA fragment of the LEU2 upstream region (P. Friden and P. Schimmel, Mol. Cell. Biol. 7:2707-2717, 1987). W...

journal_title：Molecular and cellular biology

authors： Friden P,Schimmel P

更新日期：1988-07-01 00:00:00

abstract：:The LAC9 protein of Kluyveromyces lactis is a transcriptional regulator of genes in the lactose-galactose regulon. To regulate transcription, LAC9 must bind to 17-bp upstream activator sequences (UASs) located in front of each target gene. LAC9 is homologous to the GAL4 protein of Saccharomyces cerevisiae, and the two...

journal_title：Molecular and cellular biology

authors： Halvorsen YD,Nandabalan K,Dickson RC

更新日期：1991-04-01 00:00:00

abstract：:The small subunit of ribonucleotide reductase in Saccharomyces cerevisiae (RNR2) was induced 3- to 20-fold by a variety of DNA-damaging agents. Induction of the RNR2 transcript by at least one of these agents, methyl methanesulfonate, did not require protein synthesis. To identify sequences involved in the regulation ...

journal_title：Molecular and cellular biology

authors： Hurd HK,Roberts JW

更新日期：1989-12-01 00:00:00

abstract：:Inactivation of DNA mismatch repair by mutation or by transcriptional silencing of the MLH1 gene results in genome instability and cancer predisposition. We recently found (P. V. Shcherbakova and T. A. Kunkel, Mol. Cell. Biol. 19:3177-3183, 1999) that an elevated spontaneous mutation rate can also result from increase...

journal_title：Molecular and cellular biology

authors： Shcherbakova PV,Hall MC,Lewis MS,Bennett SE,Martin KJ,Bushel PR,Afshari CA,Kunkel TA

更新日期：2001-02-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, yeast RAS proteins are potent activators of adenylate cyclase. In the present work we measured the activity of adenylate cyclase in membranes from Saccharomyces cerevisiae which overexpress this enzyme. The response of the enzyme to added RAS2 proteins bound with various guanine ...

journal_title：Molecular and cellular biology

authors： Field J,Broek D,Kataoka T,Wigler M

更新日期：1987-06-01 00:00:00

abstract：:Methyl-accepting assays and a sensitive method for labeling specific CpG sites have been used to show that the DNA of F9 embryonal carcinoma cells decreases in 5-methylcytosine content by ca. 9% during retinoic acid-induced differentiation, whereas the DNA of dimethyl sulfoxide-induced Friend murine erythroleukemia (M...

journal_title：Molecular and cellular biology

authors： Bestor TH,Hellewell SB,Ingram VM

更新日期：1984-09-01 00:00:00

abstract：:Three DNA polymerases, polymerases α, δ, and ε (Pol α, Pol δ, and Pol ε), are responsible for eukaryotic genome duplication. When DNA replication stress is encountered, DNA synthesis stalls until the stress is ameliorated. However, it is not known whether there is a difference in the association of each polymerase wit...

journal_title：Molecular and cellular biology

authors： Yu C,Gan H,Zhang Z

更新日期：2017-10-13 00:00:00

abstract：:During investigations on the regulation of the Pax-6 gene, we characterized a cDNA from quail neuroretina showing a 5' untranslated region distinct from that previously described and initiated from an internal promoter. Using RNase protection and primer extension mapping, we localized this second quail Pax-6 promoter,...

journal_title：Molecular and cellular biology

authors： Plaza S,Dozier C,Turque N,Saule S

更新日期：1995-06-01 00:00:00

abstract：:The mitogen-activated protein (MAP) kinases represent one of the most important classes of signaling cascades that are used by eukaryotic cells to sense extracellular signals. One of the major responses to these cascades is a change in cellular gene expression profiles mediated through the direct targeting of transcri...

journal_title：Molecular and cellular biology

authors： Zhang L,Yang SH,Sharrocks AD

更新日期：2007-04-01 00:00:00