Two conserved amino acid motifs mediate protein targeting to the micronemes of the apicomplexan parasite Toxoplasma gondii.

abstract：:O(6)-Methylguanine (m6G) is formed by the action of alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on DNA. m6G is a highly mutagenic and carcinogenic lesion, and it presents a block to synthesis by DNA polymerases. Here, we provide genetic and biochemical evidence for the involvement of yeast an...

journal_title：Molecular and cellular biology

authors： Haracska L,Prakash S,Prakash L

更新日期：2000-11-01 00:00:00

abstract：:We report here the isolation of a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, ESE-1 (for epithelium-specific Ets), which has features distinct from those of any other ets-related factor. ESE-1 contains two putative DNA binding domains: an ETS domain, which is unique in that ...

journal_title：Molecular and cellular biology

authors： Oettgen P,Alani RM,Barcinski MA,Brown L,Akbarali Y,Boltax J,Kunsch C,Munger K,Libermann TA

更新日期：1997-08-01 00:00:00

abstract：:2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other aryl hydrocarbon receptor (AhR) ligands suppress 17beta-estradiol (E)-induced responses in the rodent uterus and mammary tumors and in human breast cancer cells. Treatment of ZR-75, T47D, and MCF-7 human breast cancer cells with TCDD induces proteasome-dependent deg...

journal_title：Molecular and cellular biology

authors： Wormke M,Stoner M,Saville B,Walker K,Abdelrahim M,Burghardt R,Safe S

更新日期：2003-03-01 00:00:00

abstract：:Gene targeting is a very powerful tool for studying mammalian development and physiology and for creating models of human diseases. In many instances, however, it is desirable to study different modifications of a target gene, but this is limited by the generally low frequency of homologous recombination in mammalian ...

journal_title：Molecular and cellular biology

authors： Cohen-Tannoudji M,Robine S,Choulika A,Pinto D,El Marjou F,Babinet C,Louvard D,Jaisser F

更新日期：1998-03-01 00:00:00

abstract：:LEU3 of Saccharomyces cerevisiae encodes an 886-amino-acid polypeptide that regulates transcription of a group of genes involved in leucine biosynthesis and has been shown to bind specifically to a 114-base-pair DNA fragment of the LEU2 upstream region (P. Friden and P. Schimmel, Mol. Cell. Biol. 7:2707-2717, 1987). W...

journal_title：Molecular and cellular biology

authors： Friden P,Schimmel P

更新日期：1988-07-01 00:00:00

abstract：:DET1 (de-etiolated 1) is an essential negative regulator of plant light responses, and it is a component of the Arabidopsis thaliana CDD complex containing DDB1 and COP10 ubiquitin E2 variant. Human DET1 has recently been isolated as one of the DDB1- and Cul4A-associated factors, along with an array of WD40-containing...

journal_title：Molecular and cellular biology

authors： Pick E,Lau OS,Tsuge T,Menon S,Tong Y,Dohmae N,Plafker SM,Deng XW,Wei N

更新日期：2007-07-01 00:00:00

abstract：:DNA isolated from Saccharomyces cerevisiae strains carrying temperature-sensitive mutations in the CDC2 gene after incubation at the restrictive temperature contains multiple stably denatured regions 200 to 700 base pairs long. These regions are probably stabilized by a DNA-binding protein. They are found in both repl...

journal_title：Molecular and cellular biology

authors： Conrad MN,Newlon CS

更新日期：1983-09-01 00:00:00

abstract：:We have previously proposed that IQGAP1, an effector of Rac1 and Cdc42, negatively regulates cadherin-mediated cell-cell adhesion by interacting with beta-catenin and by causing the dissociation of alpha-catenin from cadherin-beta-catenin-alpha-catenin complexes and that activated Rac1 and Cdc42 positively regulate ca...

journal_title：Molecular and cellular biology

authors： Fukata M,Nakagawa M,Itoh N,Kawajiri A,Yamaga M,Kuroda S,Kaibuchi K

更新日期：2001-03-01 00:00:00

abstract：:We previously identified a small basic leucine zipper (bZIP) protein, Jun dimerization protein 2 (JDP-2), that acts as a coregulator of the N-terminal transcriptional activation domain of progesterone receptor (PR). We show here that JDP-2, through interaction with the DNA binding domain (DBD), induces or stabilizes s...

journal_title：Molecular and cellular biology

authors： Wardell SE,Kwok SC,Sherman L,Hodges RS,Edwards DP

更新日期：2005-10-01 00:00:00

abstract：:We examined the relationship between pre-mRNA splicing and the nuclear matrix by using an in vivo system that we have developed. Plasmids containing the inducible herpesvirus tk gene promoter linked to an intron-containing segment of the rabbit beta-globin gene were transfected into HeLa cells, and then the promoter w...

journal_title：Molecular and cellular biology

authors： Zeitlin S,Parent A,Silverstein S,Efstratiadis A

更新日期：1987-01-01 00:00:00

abstract：:It is well accepted that for transcriptional silencing in budding yeast, the evolutionarily conserved lysine deacetylase Sir2, in concert with its partner proteins Sir3 and Sir4, establishes a chromatin structure that prevents RNA polymerase II (Pol II) transcription. However, the mechanism of repression remains contr...

journal_title：Molecular and cellular biology

authors： Gao L,Gross DS

更新日期：2008-06-01 00:00:00

abstract：:Gamma interferon (IFN-γ) is an inflammatory cytokine that has complex effects on myogenesis. Here, we show that the IFN-γ-induced inhibition of myogenesis is mediated by the major histocompatibility complex (MHC) class II transactivator, CIITA, which binds to myogenin and inhibits its activity. In IFN-γ-treated myobla...

journal_title：Molecular and cellular biology

authors： Londhe P,Davie JK

更新日期：2011-07-01 00:00:00

abstract：:Phosphoinositides (PIPs) are present in very small amounts but are essential for cell signaling, morphogenesis, and polarity. By mass spectrometry, we demonstrated that some PIPs with stearic acyl chains were strongly disturbed in a psi1Δ Saccharomyces cerevisiae yeast strain deficient in the specific incorporation of...

journal_title：Molecular and cellular biology

authors： Doignon F,Laquel P,Testet E,Tuphile K,Fouillen L,Bessoule JJ

更新日期：2016-02-16 00:00:00

abstract：:SCIP is a POU domain transcription factor expressed by glial progenitor cells in the peripheral and central nervous systems (dividing Schwann cells and O-2A cells, respectively), where it appears to act as a repressor of myelin-specific genes. We have isolated genomic clones encoding the rat SCIP gene. Comparison of t...

journal_title：Molecular and cellular biology

authors： Kuhn R,Monuki ES,Lemke G

更新日期：1991-09-01 00:00:00

abstract：:A special group of mitochondrial outer membrane proteins spans the membrane once, exposing soluble domains to both sides of the membrane. These proteins are synthesized in the cytosol and then inserted into the membrane by an unknown mechanism. To identify proteins that are involved in the biogenesis of the single-spa...

journal_title：Molecular and cellular biology

authors： Papić D,Elbaz-Alon Y,Koerdt SN,Leopold K,Worm D,Jung M,Schuldiner M,Rapaport D

更新日期：2013-10-01 00:00:00

abstract：:Quantitative immunoelectron microscopy and subcellular fractionation established the site of endoplasmic reticulum (ER)-Golgi transport arrest induced by the phosphatase inhibitor okadaic acid (OA). OA induced the disappearance of transitional element tubules and accumulation of the anterograde-transported Chandipura ...

journal_title：Molecular and cellular biology

authors： Pryde JG,Farmaki T,Lucocq JM

更新日期：1998-02-01 00:00:00

abstract：:Akt is activated on the plasma membrane and its substrates are distributed throughout various cellular compartments. To phosphorylate its substrates, Akt needs to be recruited to specific intracellular compartments. Thus, regulation of Akt cellular compartmentalization constitutes an important mechanism to specify Akt...

journal_title：Molecular and cellular biology

authors： Ding J,Du K

更新日期：2009-03-01 00:00:00

abstract：:As shown by Southern blot analysis, the metallothionein-1 (MT-1) genes in rats comprise a multigene family. We present the sequence of the MT-1 structural gene and compare its features with other metallothionein genes. Three MT-1 pseudogenes which we sequenced apparently arose by reverse transcription of processed mRN...

journal_title：Molecular and cellular biology

authors： Andersen RD,Birren BW,Taplitz SJ,Herschman HR

更新日期：1986-01-01 00:00:00

abstract：:The induction of the granulocytic differentiation of leukemic cells by all-trans retinoic acid (RA) has been a major breakthrough in terms of survival for acute promyelocytic leukemia (APL) patients. Here we highlight the synergism and the underlying novel mechanism between RA and the granulocyte colony-stimulating fa...

journal_title：Molecular and cellular biology

authors： Cassinat B,Zassadowski F,Ferry C,Llopis L,Bruck N,Lainey E,Duong V,Cras A,Despouy G,Chourbagi O,Beinse G,Fenaux P,Rochette Egly C,Chomienne C

更新日期：2011-04-01 00:00:00

abstract：:Histone acetylation and deacetylation have been implicated in the regulation of gene expression. Molecular studies have shown that histone deacetylases (HDACs) function as transcriptional repressors. However, very little is known about their roles during development in multicellular organisms. We previously demonstrat...

journal_title：Molecular and cellular biology

authors： Dufourcq P,Victor M,Gay F,Calvo D,Hodgkin J,Shi Y

更新日期：2002-05-01 00:00:00

abstract：:The baker's yeast Saccharomyces cerevisiae possesses a single gene encoding heat shock transcription factor (HSF), which is required for the activation of genes that participate in stress protection as well as normal growth and viability. Yeast HSF (yHSF) contains two distinct transcriptional activation regions locate...

journal_title：Molecular and cellular biology

authors： Santoro N,Johansson N,Thiele DJ

更新日期：1998-11-01 00:00:00

abstract：:Pseudo-exons are intronic sequences that are flanked by apparent consensus splice sites but that are not observed in spliced mRNAs. Pseudo-exons are often difficult to activate by mutation and have typically been viewed as a conceptual challenge to our understanding of how the spliceosome discriminates between authent...

journal_title：Molecular and cellular biology

authors： Grellscheid SN,Smith CW

更新日期：2006-03-01 00:00:00

abstract：:Nucleolin is a ubiquitous multifunctional protein involved in preribosome assembly and associated with both nucleolar chromatin in interphase and nucleolar organizer regions on metaphasic chromosomes in mitosis. Extensive nucleolin phosphorylation by a casein kinase (CKII) occurs on serine in growing cells. Here we re...

journal_title：Molecular and cellular biology

authors： Belenguer P,Caizergues-Ferrer M,Labbé JC,Dorée M,Amalric F

更新日期：1990-07-01 00:00:00

abstract：:Protein tyrosine kinase Pyk2 is activated by a variety of G-protein-coupled receptors and by extracellular signals that elevate intracellular Ca2+ concentration. We have identified a new Pyk2 binding protein designated Pap. Pap is a multidomain protein composed of an N-terminal alpha-helical region with a coiled-coil ...

journal_title：Molecular and cellular biology

authors： Andreev J,Simon JP,Sabatini DD,Kam J,Plowman G,Randazzo PA,Schlessinger J

更新日期：1999-03-01 00:00:00

abstract：:The Caenorhabditis elegans run gene encodes a Runt domain factor. Runx1, Runx2, and Runx3 are the three known mammalian homologs of run. Runx1, which plays an essential role in hematopoiesis, has been identified at the breakpoint of chromosome translocations that are responsible for human leukemia. Runx2 plays an esse...

journal_title：Molecular and cellular biology

authors： Nam S,Jin YH,Li QL,Lee KY,Jeong GB,Ito Y,Lee J,Bae SC

更新日期：2002-01-01 00:00:00

abstract：:Phosphatidylinositol (PI) 3-kinase is a cytoplasmic signaling molecule recruited to the membrane by activated growth factor receptors. The p85 subunit of PI 3-kinase links the catalytic p110 subunit to activated growth factor receptors and is required for enzymatic activity of p110. In this report, we describe the eff...

journal_title：Molecular and cellular biology

authors： Klippel A,Reinhard C,Kavanaugh WM,Apell G,Escobedo MA,Williams LT

更新日期：1996-08-01 00:00:00

abstract：:In an effort to identify widely active positive regulatory elements, we have examined the action of the cytomegalovirus enhancer-promoter in transgenic mice. These elements activated expression in 24 of 28 tissues tested. The greatest expression was observed in the heart, kidney, brain, and testis. Maximum expression ...

journal_title：Molecular and cellular biology

authors： Schmidt EV,Christoph G,Zeller R,Leder P

更新日期：1990-08-01 00:00:00

abstract：:Ret/ptc2 is a constitutively active, oncogenic form of the c-Ret receptor tyrosine kinase. Like the other papillary thyroid carcinoma forms of Ret, Ret/ptc2 is activated through fusion of the Ret tyrosine kinase domain to the dimerization domain of another protein. Investigation of requirements for Ret/ptc2 mitogenic ...

journal_title：Molecular and cellular biology

authors： Durick K,Gill GN,Taylor SS

更新日期：1998-04-01 00:00:00

abstract：:The cytotoxic serine protease B (CSP-B) gene is activated during cytotoxic T-lymphocyte maturation. In this report, we demonstrate that the PEER T-cell line (bearing gamma/delta T-cell receptors) accumulates CSP-B mRNA following exposure to 12-O-tetradecanoylphorbol-13-acetate (TPA) and N6-2'-O-dibutyryladenosine 3',5...

journal_title：Molecular and cellular biology

authors： Hanson RD,Ley TJ

更新日期：1990-11-01 00:00:00

abstract：:The death domain-containing receptor superfamily and their respective downstream mediators control whether or not cells initiate apoptosis or activate NF-kappaB, events critical for proper immune system function. A screen for upstream activators of NF-kappaB identified a novel serine-threonine kinase capable of activa...

journal_title：Molecular and cellular biology

authors： Pazdernik NJ,Donner DB,Goebl MG,Harrington MA

更新日期：1999-10-01 00:00:00