Abstract:
:Phosphoinositides (PIPs) are present in very small amounts but are essential for cell signaling, morphogenesis, and polarity. By mass spectrometry, we demonstrated that some PIPs with stearic acyl chains were strongly disturbed in a psi1Δ Saccharomyces cerevisiae yeast strain deficient in the specific incorporation of a stearoyl chain at the sn-1 position of phosphatidylinositol. The absence of PIPs containing stearic acid induced disturbances in intracellular trafficking, although the total amount of PIPs was not diminished. Changes in PIPs also induced alterations in the budding pattern and defects in actin cytoskeleton organization (cables and patches). Moreover, when the PSI1 gene was impaired, a high proportion of cells with bipolar cortical actin patches that occurred concomitantly with the bipolar localization of Cdc42p was specifically found among diploid cells. This bipolar cortical actin phenotype, never previously described, was also detected in a bud9Δ/bud9Δ strain. Very interestingly, overexpression of PSI1 reversed this phenotype.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Doignon F,Laquel P,Testet E,Tuphile K,Fouillen L,Bessoule JJdoi
10.1128/MCB.00843-15subject
Has Abstractpub_date
2016-02-16 00:00:00pages
765-80issue
5eissn
0270-7306issn
1098-5549pii
MCB.00843-15journal_volume
36pub_type
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