A model system for activation-induced alternative splicing of CD45 pre-mRNA in T cells implicates protein kinase C and Ras.

abstract：:We have examined the ability of the simian virus 40 72-base pair enhancer segment to simultaneously activate multiple transcription units with plasmids that contain one, two, or three simian virus 40-based transcription units in various arrangements. After transfection into CV1 cells, the expression of a marker gene, ...

journal_title：Molecular and cellular biology

authors： Kadesch T,Berg P

更新日期：1986-07-01 00:00:00

abstract：:Retinoblastoma (RB) tumors develop when both alleles of a gene (RB1) are mutated and unable to function normally. Recently, Friend et al. [S. H. Friend, R. Bernards, S. Rogelj, R. A. Weinberg, J. M. Rapaport, D. M. Albert, and T. P. Dryja, Nature (London) 32:643-646, 1986] reported the cloning of a gene, 4.7R, with so...

journal_title：Molecular and cellular biology

authors： Goddard AD,Balakier H,Canton M,Dunn J,Squire J,Reyes E,Becker A,Phillips RA,Gallie BL

更新日期：1988-05-01 00:00:00

abstract：:We describe a genetic transformation system which should prove useful for investigating tropomyosin assembly and function. Muscle abnormalities associated with a defective tropomyosin allele were corrected by integrating the wild-type gene into germ line chromosomes. The transformation protocol permits application of ...

journal_title：Molecular and cellular biology

authors： Fyrberg EA,Karlik CC

更新日期：1987-08-01 00:00:00

abstract：:Tristetraprolin (TTP) acts by binding to AU-rich elements in certain mRNAs, such as tumor necrosis factor (TNF) mRNA, and increasing their decay rates. TTP knockout mice exhibit a profound inflammatory syndrome that is largely due to increased TNF levels. Although TTP's effects on gene expression have been well studie...

journal_title：Molecular and cellular biology

authors： Patial S,Stumpo DJ,Young WS 3rd,Ward JM,Flake GP,Blackshear PJ

更新日期：2016-04-15 00:00:00

abstract：:The C proteins (C1 and C2) of HeLa 40S heterogeneous nuclear ribonucleoprotein particles copurify under native conditions as a stable complex with a fixed molar protein ratio (S.F. Barnett, W.M. LeStourgeon, and D.L. Friedman, J. Biochem. Biophys. Methods 16:87-97, 1988). Gel filtration chromatography and velocity sed...

journal_title：Molecular and cellular biology

authors： Barnett SF,Friedman DL,LeStourgeon WM

更新日期：1989-02-01 00:00:00

abstract：:In Saccharomyces cerevisiae (budding yeast), commitment to cell division in late G(1) is promoted by the G(1) cyclin Cln3 and its associated cyclin-dependent kinase, Cdc28. We show here that all known aspects of the function of Cln3 in G(1) phase, including control of cell size, pheromone sensitivity, cell cycle progr...

journal_title：Molecular and cellular biology

authors： Wijnen H,Landman A,Futcher B

更新日期：2002-06-01 00:00:00

abstract：:The highly conserved SR family contains a growing number of phosphoproteins acting as both essential and alternative splicing factors. In this study, we have cloned human genomic and cDNA sequences encoding a novel SR protein designated SRp46. Nucleotide sequence analyses have revealed that the SRp46 gene corresponds ...

journal_title：Molecular and cellular biology

authors： Soret J,Gattoni R,Guyon C,Sureau A,Popielarz M,Le Rouzic E,Dumon S,Apiou F,Dutrillaux B,Voss H,Ansorge W,Stévenin J,Perbal B

更新日期：1998-08-01 00:00:00

abstract：:Eukaryotic splicing factors belonging to the SR family are essential splicing factors consisting of an N-terminal RNA-binding region and a C-terminal RS domain. They are believed to be involved in alternative splicing of numerous transcripts because their expression levels can influence splice site selection. We have ...

journal_title：Molecular and cellular biology

authors： Jumaa H,Guénet JL,Nielsen PJ

更新日期：1997-06-01 00:00:00

abstract：:Both cyclin D1 and the transcription factor C/EBPβ are required for mammary epithelial cell differentiation; however, the pathway in which they operate is uncertain. Previous analyses of the patterns of gene expression in human tumors suggested a connection between cyclin D1 overexpression and C/EBPβ, but whether this...

journal_title：Molecular and cellular biology

authors： Liu Q,Boudot A,Ni J,Hennessey T,Beauparlant SL,Rajabi HN,Zahnow C,Ewen ME

更新日期：2014-08-01 00:00:00

abstract：:Combined deficiencies of poly(ADP)ribosyl polymerase 1 (PARP1) and ataxia telangiectasia mutated (ATM) result in synthetic lethality and, in the mouse, early embryonic death. Here, we investigated the genetic requirements for this lethality via analysis of mice deficient for PARP1 and either of two ATM-regulated DNA d...

journal_title：Molecular and cellular biology

authors： Orsburn B,Escudero B,Prakash M,Gesheva S,Liu G,Huso DL,Franco S

更新日期：2010-05-01 00:00:00

abstract：:Translocation of preproteins across the mitochondrial outer membrane is mediated by the TOM complex. This complex consists of receptor components for the initial contact with preproteins at the mitochondrial surface and membrane-embedded proteins which promote transport and form the translocation pore. In order to und...

journal_title：Molecular and cellular biology

authors： Rapaport D,Künkele KP,Dembowski M,Ahting U,Nargang FE,Neupert W,Lill R

更新日期：1998-09-01 00:00:00

abstract：:mRNA deadenylation is a key process in the regulation of translation and mRNA turnover. In Saccharomyces cerevisiae, deadenylation is primarily carried out by the Ccr4p and Caf1p/Pop2p subunits of the Ccr4-Not complex, which is conserved in eukaryotes including humans. Here we have identified an unconventional human C...

journal_title：Molecular and cellular biology

authors： Wagner E,Clement SL,Lykke-Andersen J

更新日期：2007-03-01 00:00:00

abstract：:We analyzed the replication of two unlinked actin genes, ardB and ardC , which are abundantly transcribed in the naturally synchronous plasmodium of the slime mold Physarum polycephalum. Detection and size measurements of single-stranded nascent replication intermediates (RIs) demonstrate that these two genes are conc...

journal_title：Molecular and cellular biology

authors： Bénard M,Lagnel C,Pallotta D,Pierron G

更新日期：1996-03-01 00:00:00

abstract：:ZO-1, ZO-2, and ZO-3 are closely related MAGUK family proteins that localize at the cytoplasmic surface of tight junctions (TJs). ZO-1 and ZO-2 are expressed in both epithelia and endothelia, whereas ZO-3 is exclusively expressed in epithelia. In spite of intensive studies of these TJ MAGUKs, our knowledge of their fu...

journal_title：Molecular and cellular biology

authors： Adachi M,Inoko A,Hata M,Furuse K,Umeda K,Itoh M,Tsukita S

更新日期：2006-12-01 00:00:00

abstract：:Phorbol-12-myristate-13-acetate (PMA), a potent tumor promoter, was shown to have opposite effects on the cellular morphology and steady-state levels of beta-actin mRNA in embryonic chicken muscle fibroblasts and sternal chondrocytes. When fibroblasts were treated with PMA, they formed foci of densely packed cells, ce...

journal_title：Molecular and cellular biology

authors： Gerstenfeld LC,Finer MH,Boedtker H

更新日期：1985-06-01 00:00:00

abstract：:In addition to the highly conserved AATAAA sequence, there is a requirement for specific sequences downstream of polyadenylic acid [poly(A)] cleavage sites to generate correct mRNA 3' termini. Previous experiments demonstrated that 35 nucleotides downstream of the E2A poly(A) site were sufficient but 20 nucleotides we...

journal_title：Molecular and cellular biology

authors： Hart RP,McDevitt MA,Ali H,Nevins JR

更新日期：1985-11-01 00:00:00

abstract：:A Dictyostelium discoideum cDNA encoding an alpha-type subunit of casein kinase II was isolated, and its cDNA was used to study developmental expression of casein kinase II during the Dictyostelium life cycle. The 1.3-kb cDNA insert contained an open reading frame of 337 amino acids (M(r) 39,900). The deduced amino ac...

journal_title：Molecular and cellular biology

authors： Kikkawa U,Mann SK,Firtel RA,Hunter T

更新日期：1992-12-01 00:00:00

abstract：:E3 ubiquitin ligases, which target specific molecules for proteolytic destruction, have emerged as key regulators of immune functions. Several E3 ubiquitin ligases, including c-Cbl, Cbl-b, GRAIL, Itch, and Nedd4, have been shown to negatively regulate T-cell activation. Here, we report that the HECT-type E3 ligase AIP...

journal_title：Molecular and cellular biology

authors： Chen A,Gao B,Zhang J,McEwen T,Ye SQ,Zhang D,Fang D

更新日期：2009-10-01 00:00:00

abstract：:We have cloned and characterized the gene encoding the late histone H1-beta subtype from the sea urchin Strongylocentrotus purpuratus. The gene contains all of the upstream sequence homologies previously seen in late H1-gamma genes. The expression of H1-beta mRNA is coordinated with that of H1-gamma mRNA, and like H1-...

journal_title：Molecular and cellular biology

authors： Lai ZC,Childs G

更新日期：1988-04-01 00:00:00

abstract：:We have characterized the putative AP1 site in the backbone of pUC plasmids and found unique regulatory effects. The site, which mapped to a 19-bp region around nucleotide 37, conferred transcriptional activation by Jun or Jun/Fos that was boosted up to fivefold by unliganded thyroid hormone receptor (TR). Thyroid hor...

journal_title：Molecular and cellular biology

authors： Lopez G,Schaufele F,Webb P,Holloway JM,Baxter JD,Kushner PJ

更新日期：1993-05-01 00:00:00

abstract：:We have identified a complex between TFIIIB and the upstream promoter of silkworm tRNA Ala genes that is detectable by gel retardation and DNase I footprinting. Formation of this complex depends on the integrity of previously identified upstream promoter elements and on the presence of other silkworm transcription fac...

journal_title：Molecular and cellular biology

authors： Young LS,Ahnert N,Sprague KU

更新日期：1996-03-01 00:00:00

abstract：:Phospholipase C-gamma2 (PLC-gamma2) plays an important role in B-cell signaling. Phosphorylation of various tyrosine residues of PLC-gamma2 has been implicated in regulation of its lipase activity. With the use of antibodies specific for each of the putative phosphorylation sites, we have now shown that PLC-gamma2 is ...

journal_title：Molecular and cellular biology

authors： Kim YJ,Sekiya F,Poulin B,Bae YS,Rhee SG

更新日期：2004-11-01 00:00:00

abstract：:The exons, their boundaries, and approximately half of the intronic deoxyribonucleic acid of the rat serum albumin gene were sequenced. In addition to the 14 exons identified earlier by R-loop analysis, a small exon was detected between the "leader" exon (Z) and exon B. The leader exon encoded the 5'-untranslated port...

journal_title：Molecular and cellular biology

authors： Sargent TD,Jagodzinski LL,Yang M,Bonner J

更新日期：1981-10-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the Wee1 family kinase Swe1p is normally stable during G(1) and S phases but is unstable during G(2) and M phases due to ubiquitination and subsequent degradation. However, perturbations of the actin cytoskeleton lead to a stabilization and accumulation of Swe1p. This response constitutes ...

journal_title：Molecular and cellular biology

authors： McMillan JN,Longtine MS,Sia RA,Theesfeld CL,Bardes ES,Pringle JR,Lew DJ

更新日期：1999-10-01 00:00:00

abstract：:The DNA damage checkpoint is tightly controlled. After its activation, the checkpoint machinery is inactivated once lesions are repaired or undergoes adaptation if the DNA damage is unable to be repaired. Protein acetylation has been shown to play an important role in DNA damage checkpoint activation. However, the rol...

journal_title：Molecular and cellular biology

authors： Tao R,Xue H,Zhang J,Liu J,Deng H,Chen YG

更新日期：2013-11-01 00:00:00

abstract：:IME1 encodes a transcriptional activator required for the transcription of meiosis-specific genes and initiation of meiosis in Saccharomyces cerevisiae. The transcription of IME1 is repressed in the presence of glucose, and a low basal level of IME1 RNA is observed in vegetative cultures with acetate as the sole carbo...

journal_title：Molecular and cellular biology

authors： Sagee S,Sherman A,Shenhar G,Robzyk K,Ben-Doy N,Simchen G,Kassir Y

更新日期：1998-04-01 00:00:00

abstract：:In neural development, Notch signaling plays a key role in restricting neuronal differentiation, promoting the maintenance of progenitor cells. Classically, Notch signaling causes transactivation of Hairy-enhancer of Split (HES) genes which leads to transcriptional repression of neural determination and differentiatio...

journal_title：Molecular and cellular biology

authors： Sriuranpong V,Borges MW,Strock CL,Nakakura EK,Watkins DN,Blaumueller CM,Nelkin BD,Ball DW

更新日期：2002-05-01 00:00:00

abstract：:Our previous studies of Saccharomyces cerevisiae described a gene repression mechanism where the transcription of intergenic noncoding DNA (ncDNA) (SRG1) assembles nucleosomes across the promoter of the adjacent SER3 gene that interfere with the binding of transcription factors. To investigate the role of histones in ...

journal_title：Molecular and cellular biology

authors： Hainer SJ,Martens JA

更新日期：2011-09-01 00:00:00

abstract：:In self-renewing tissues such as the skin epidermis and the bone marrow, Myc proteins control differentiation of stem cells and proliferation of progenitor cell types. In the epithelium of the small intestine, we show that c-Myc and N-Myc are expressed in a differential manner. Whereas c-Myc is expressed in the prolif...

journal_title：Molecular and cellular biology

authors： Bettess MD,Dubois N,Murphy MJ,Dubey C,Roger C,Robine S,Trumpp A

更新日期：2005-09-01 00:00:00

abstract：:Axin is a recently identified protein encoded by the fused locus in mice that is required for normal vertebrate axis formation. We have defined a 25-amino-acid sequence in axin that comprises the glycogen synthase kinase 3beta (GSK-3beta) interaction domain (GID). In contrast to full-length axin, which has been shown ...

journal_title：Molecular and cellular biology

authors： Hedgepeth CM,Deardorff MA,Rankin K,Klein PS

更新日期：1999-10-01 00:00:00