Abstract:
:We have identified a complex between TFIIIB and the upstream promoter of silkworm tRNA Ala genes that is detectable by gel retardation and DNase I footprinting. Formation of this complex depends on the integrity of previously identified upstream promoter elements and on the presence of other silkworm transcription factors, either TFIIID or a fraction that contains both TFIIIC and TFIIID. We have used this complex to compare the interactions of TFIIIB with two kinds of tRNA Ala genes whose different in vitro transcription properties are conferred by the upstream segments of their promoters. These are the tRNA C Ala genes, which are transcribed constitutively, and the tRNA SG Ala genes, which are transcribed only in the silk gland. We find that TFIIIB binds tRNA SG Ala genes with lower affinity than it binds tRNA C Ala genes. In addition, the TFIIIB complex formed on tRNA SG Ala genes differ qualitatively from those formed on tRNA C Ala genes. Both the transcriptional activity of tRNA SG Ala complexes and the ability of the complexes to protect upstream DNA from DNase I digestion are reduced.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Young LS,Ahnert N,Sprague KUdoi
10.1128/mcb.16.3.1256subject
Has Abstractpub_date
1996-03-01 00:00:00pages
1256-66issue
3eissn
0270-7306issn
1098-5549journal_volume
16pub_type
杂志文章abstract::Axin is a recently identified protein encoded by the fused locus in mice that is required for normal vertebrate axis formation. We have defined a 25-amino-acid sequence in axin that comprises the glycogen synthase kinase 3beta (GSK-3beta) interaction domain (GID). In contrast to full-length axin, which has been shown ...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/mcb.8.2.540
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/mcb.14.1.543
更新日期:1994-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1992-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/MCB.25.13.5543-5551.2005
更新日期:2005-07-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.06473-11
更新日期:2012-02-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.10.3.1049
更新日期:1990-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.18.7.3681
更新日期:1998-07-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.14.9.6171
更新日期:1994-09-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.17.1.495
更新日期:1997-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/MCB.21.2.524-533.2001
更新日期:2001-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1981-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-05-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-10-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.5.7.1649
更新日期:1985-07-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.9.4.1389
更新日期:1989-04-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.16.10.5857
更新日期:1996-10-01 00:00:00