Abstract:
:In the gal-his3 hybrid promoter his3-GG1, the yeast upstream activator protein GCN4 stimulates transcription when bound at the position normally occupied by the TATA element. This TATA-independent activation by GCN4 requires two additional elements in the gal enhancer region that are distinct from those involved in normal galactose induction. Both additional elements appear to be functionally distinct from a classical TATA element because they cannot be replaced by the TFIID-binding sequence TATAAA. One of these elements, termed Q, is essential for GCN4-activated transcription and contains the sequence GTCAC CCG, which overlaps (but is distinct from) a GAL4 binding site. Surprisingly, relatively small increases in the distance between Q and the GCN4 binding site significantly reduce the level of transcription. The Q element specifically interacts with a yeast protein (Q-binding protein [QBP]) that may be equivalent to Y, a protein that binds at a sequence that forms a constraint to nucleosome positioning. Analysis of various deletion mutants indicates that the sequence requirements for binding by QBP in vitro are indistinguishable from those necessary for Q activity in vivo, strongly suggesting that QBP is required for the function of this TATA-independent promoter. These results support the view that transcriptional activation can occur by an alternative mechanism in which the TATA-binding factor TFIID either is not required or is not directly bound to DNA. In addition, they suggest a potential role of nucleosome positioning for the activity of a promoter.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Brandl CJ,Struhl Kdoi
10.1128/mcb.10.8.4256subject
Has Abstractpub_date
1990-08-01 00:00:00pages
4256-65issue
8eissn
0270-7306issn
1098-5549journal_volume
10pub_type
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1998-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/mcb.12.8.3583
更新日期:1992-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.24.1.377-388.2004
更新日期:2004-01-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.19.8.5289
更新日期:1999-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01325-08
更新日期:2009-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-08-01 00:00:00