Co-amplification of rRNA genes with CAD genes in N-(phosphonacetyl)-L-aspartate-resistant Syrian hamster cells.

abstract：:The mSin3A-histone deacetylase corepressor is a multiprotein complex that is recruited by DNA binding transcriptional repressors. Sin3 has four paired amphipathic alpha helices (PAH1 to -4) that are protein-protein interaction motifs and is the scaffold upon which the complex assembles. We identified a novel mSin3A-in...

journal_title：Molecular and cellular biology

authors： Yochum GS,Ayer DE

更新日期：2001-07-01 00:00:00

abstract：:Ribosomal (r) proteins encoded by polyadenylated RNA were specifically precipitated in vitro from polysomes by using antibodies raised against characterized Drosophila melanogaster r proteins. The immuno-purified mRNA in the polysome complex was used to prepare cDNA with which to probe a D. melanogaster genomic librar...

journal_title：Molecular and cellular biology

authors： Burns DK,Stark BC,Macklin MD,Chooi WY

更新日期：1984-12-01 00:00:00

abstract：:Smads 1, 5, and 8 are the intracellular mediators for the bone morphogenetic proteins (BMPs), which play crucial roles during mammalian development. Previous research has shown that Smad1 is important in the formation of the allantois, while Smad5 has been shown to be critical in the process of angiogenesis. To furthe...

journal_title：Molecular and cellular biology

authors： Hester M,Thompson JC,Mills J,Liu Y,El-Hodiri HM,Weinstein M

更新日期：2005-06-01 00:00:00

abstract：:The Sry-related high-mobility-group box transcription factor Sox9 recruits the redundant L-Sox5 and Sox6 proteins to effect chondrogenesis, but the mode of action of the trio remains unclear. We identify here a highly conserved 359-bp sequence 10 kb upstream of the Agc1 gene for aggrecan, a most essential cartilage pr...

journal_title：Molecular and cellular biology

authors： Han Y,Lefebvre V

更新日期：2008-08-01 00:00:00

abstract：:The extracellular signal-regulated kinase 1 and 2 (ERK1/2) mitogen-activated protein (MAP) kinase signaling pathway plays an important role in the proliferative response of mammalian cells to mitogens. However, the individual contribution of the isoforms ERK1 and ERK2 to cell proliferation control is unclear. The two ...

journal_title：Molecular and cellular biology

authors： Voisin L,Saba-El-Leil MK,Julien C,Frémin C,Meloche S

更新日期：2010-06-01 00:00:00

abstract：:The generation of two daughter cells with the same genetic information requires error-free chromosome segregation during mitosis. Chromosome transmission fidelity is dependent on spindle structure/function, which requires Asp1 in the fission yeast Schizosaccharomyces pombe Asp1 belongs to the diphosphoinositol pentaki...

journal_title：Molecular and cellular biology

authors： Pascual-Ortiz M,Saiardi A,Walla E,Jakopec V,Künzel NA,Span I,Vangala A,Fleig U

更新日期：2018-04-16 00:00:00

abstract：:TIN2 is an important regulator of telomere length, and mutations in TINF2, the gene encoding TIN2, cause short-telomere syndromes. While the genetics underscore the importance of TIN2, the mechanism through which TIN2 regulates telomere length remains unclear. Here, we tested the effects of human TIN2 on telomerase ac...

journal_title：Molecular and cellular biology

authors： Pike AM,Strong MA,Ouyang JPT,Greider CW

更新日期：2019-10-11 00:00:00

abstract：:CCAAT/enhancer-binding protein alpha (C/EBPalpha) is one of the key transcription factors that mediate lineage specification and differentiation of multipotent myeloid progenitors into mature granulocytes. Although C/EBPalpha is known to induce granulopoiesis while suppressing monocyte differentiation, it is unclear h...

journal_title：Molecular and cellular biology

authors： Ross SE,Radomska HS,Wu B,Zhang P,Winnay JN,Bajnok L,Wright WS,Schaufele F,Tenen DG,MacDougald OA

更新日期：2004-01-01 00:00:00

abstract：:The Saccharomyces cerevisiae Srs2 protein is involved in DNA repair and recombination. In order to gain better insight into the roles of Srs2, we performed a screen to identify mutations that are synthetically lethal with an srs2 deletion. One of them is a mutated allele of the ULP1 gene that encodes a protease specif...

journal_title：Molecular and cellular biology

authors： Soustelle C,Vernis L,Fréon K,Reynaud-Angelin A,Chanet R,Fabre F,Heude M

更新日期：2004-06-01 00:00:00

abstract：:The CYC1-239-O mutation in the yeast Saccharomyces cerevisiae produces a -His-Leu- replacement of the normal -Ala-Gly- sequence at amino acid positions 5 and 6, which lie within a dispensable region of iso-1-cytochrome c; this mutation can accommodate the formation of a hairpin structure at the corresponding site in t...

journal_title：Molecular and cellular biology

authors： Baim SB,Pietras DF,Eustice DC,Sherman F

更新日期：1985-08-01 00:00:00

abstract：:The regulation of a thyroid hormone-responsive gene in rats, designated spot 14, was explored. The expression of this gene in liver is rapidly (less than 10 min) and markedly (greater than 10-fold) altered by the administration of 3,5,3'-triiodo-L-thyronine (T3) to hypothyroid rats (P. Narayan, C. W. Liaw, and H. C. T...

journal_title：Molecular and cellular biology

authors： Narayan P,Towle HC

更新日期：1985-10-01 00:00:00

abstract：:Colon carcinomas appear to arise from the cumulative effect of mutations to several genes (APC, DCC, p53, ras, hMLH1, and hMSH2). By using novel colonic epithelial cell lines derived from the Immorto mouse, named the YAMC (young adult mouse colon) cell line, and an Immorto-Min mouse hybrid, named the IMCE (Immorto-Min...

journal_title：Molecular and cellular biology

authors： D'Abaco GM,Whitehead RH,Burgess AW

更新日期：1996-03-01 00:00:00

abstract：:The first processing step in the maturation of mouse precursor rRNA involves cleavage at nucleotide ca. +650, at the 5' border of a 200-nucleotide region that is conserved across mammals and contains the sequences that direct the processing. To identify the relevant sequence elements, we used rRNAs with small internal...

journal_title：Molecular and cellular biology

authors： Craig N,Kass S,Sollner-Webb B

更新日期：1991-01-01 00:00:00

abstract：:Two-dimensional protein gels were used to systematically assess changes in gene expression in Friend erythroleukemia cells after exposure to inducers of differentiation. A rapid decrease in expression of the stress protein HSP70 was observed after exposure to inducers. The kinetics of this change suggest that it may b...

journal_title：Molecular and cellular biology

authors： Hensold JO,Housman DE

更新日期：1988-05-01 00:00:00

abstract：:Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1 (MBF1) was originally identified as a bridging molecule that connects the Drosophila nuclear receptor FTZ-F1 and TATA-bin...

journal_title：Molecular and cellular biology

authors： Takemaru K,Harashima S,Ueda H,Hirose S

更新日期：1998-09-01 00:00:00

abstract：:A significant challenge to our understanding of eukaryotic transcriptional regulation is to determine how multiple signal transduction pathways converge on a single promoter to regulate transcription in divergent fashions. To study this, we have investigated the transcriptional regulation of the Schizosaccharomyces po...

journal_title：Molecular and cellular biology

authors： Neely LA,Hoffman CS

更新日期：2000-09-01 00:00:00

abstract：:The expression and distribution of DNA polymerase alpha was measured by cytometry and confocal laser scanning microscopy. Expression was proportional to DNA content in proliferating cells, while only S-phase cells retained DNA polymerase alpha after detergent extraction. Nuclear DNA polymerase alpha binding may be one...

journal_title：Molecular and cellular biology

authors： Stokke T,Erikstein B,Holte H,Funderud S,Steen HB

更新日期：1991-06-01 00:00:00

abstract：:Flk2/Flt3 is a recently identified receptor tyrosine kinase expressed in brain, placenta, testis, and primitive hematopoietic cells. The mitogenic signalling potential and biochemical properties of Flk2/Flt3 have been analyzed by using a chimeric receptor composed of the extracellular domain of the human colony-stimul...

journal_title：Molecular and cellular biology

authors： Dosil M,Wang S,Lemischka IR

更新日期：1993-10-01 00:00:00

abstract：:Complete replication of the genome is an essential prerequisite for normal cell division, but a variety of factors can block the replisome, triggering replication stress and potentially causing mutation or cell death. The cellular response to replication stress involves recruitment of proteins to stabilize the replica...

journal_title：Molecular and cellular biology

authors： Her J,Ray C,Altshuler J,Zheng H,Bunting SF

更新日期：2018-03-29 00:00:00

abstract：:Substitution of phenylalanine for tyrosine at codon 809 (Y809F) of the human colony-stimulating factor 1 (CSF-1) receptor (CSF-1R) impairs ligand-stimulated tyrosine kinase activity, prevents induction of c-MYC and cyclin D1 genes, and blocks CSF-1-dependent progression through the G1 phase of the cell cycle. We devis...

journal_title：Molecular and cellular biology

authors： Davis JN,Rock CO,Cheng M,Watson JB,Ashmun RA,Kirk H,Kay RJ,Roussel MF

更新日期：1997-12-01 00:00:00

abstract：:Host cell cycle genes provide important functions to retroviruses and retroviruslike elements. To define some of these functions, the cell cycle dependence of transposition of the yeast retroviruslike element Ty3 was examined. Ty3 is unique among retroviruslike elements because of the specificity of its integration, w...

journal_title：Molecular and cellular biology

authors： Menees TM,Sandmeyer SB

更新日期：1994-12-01 00:00:00

abstract：:EYA1 is known to be overexpressed in human breast cancer, in which the Myc protein is also accumulated in association with decreased phospho-T58 (pT58) levels. We have recently reported that EYA1 functions as a unique protein phosphatase to dephosphorylate Myc at pT58 to regulate Myc levels. However, it remains unclea...

journal_title：Molecular and cellular biology

authors： Li J,Rodriguez Y,Cheng C,Zeng L,Wong EYM,Xu CY,Zhou MM,Xu PX

更新日期：2016-12-19 00:00:00

abstract：:The levels of the A, B, and C subunits of protein phosphatase 2A in extracts from synchronized embryonic bovine tracheal cells were determined by immunoblotting with subunit-specific antibodies. A constant amount of each subunit was found in resting cells as well as in growing cells from all stages of the cell cycle. ...

journal_title：Molecular and cellular biology

authors： Ruediger R,Van Wart Hood JE,Mumby M,Walter G

更新日期：1991-08-01 00:00:00

abstract：:We examined the effects of 18 h of incubation of Chinese hamster ovary (CHO K1) cells with cycloheximide, hydroxyurea, and aphidicolin. Treatment of cells with cycloheximide alone at a concentration adequate to inhibit DNA synthesis to less than 10% of control was significantly less cytotoxic and clastogenic than trea...

journal_title：Molecular and cellular biology

authors： Sherwood SW,Schumacher RI,Schimke RT

更新日期：1988-07-01 00:00:00

abstract：:The fgr proto-oncogene encodes a nonreceptor protein-tyrosine kinase, designated p55c-fgr. In this study, we have isolated human fgr cDNA molecules from normal monocyte mRNA templates. Nucleotide sequence analysis of the longest fgr cDNA revealed a 5' untranslated region of 927 bp which included two Alu-like repeats a...

journal_title：Molecular and cellular biology

authors： Gutkind JS,Link DC,Katamine S,Lacal P,Miki T,Ley TJ,Robbins KC

更新日期：1991-03-01 00:00:00

abstract：:We have shown previously that perturbation of origin firing in chromosome replication causes DNA lesions and triggers DNA damage checkpoint control, which ensures genomic integrity by stopping cell cycle progression until the lesions are repaired or by inducing cell death if they are not properly repaired. This was ba...

journal_title：Molecular and cellular biology

authors： Ide S,Watanabe K,Watanabe H,Shirahige K,Kobayashi T,Maki H

更新日期：2007-01-01 00:00:00

abstract：:Human ras genes play central roles in coupling extracellular signals with complex intracellular networks controlling proliferation, differentiation, and apoptosis, among others processes. c-H-ras pre-mRNA can be alternatively processed into two mRNAs due to the inclusion or exclusion of the alternative exon IDX; this ...

journal_title：Molecular and cellular biology

authors： Guil S,Gattoni R,Carrascal M,Abián J,Stévenin J,Bach-Elias M

更新日期：2003-04-01 00:00:00

abstract：:Telomerase extends chromosome ends by the synthesis of tandem simple-sequence repeats. Studies of minimal recombinant telomerase ribonucleoprotein (RNP) reconstituted in vitro have revealed sequences within the telomerase RNA subunit (TER) that are required to establish its internal template and other unique features ...

journal_title：Molecular and cellular biology

authors： Cunningham DD,Collins K

更新日期：2005-06-01 00:00:00

abstract：:The rad18 gene of Schizosaccharomyces pombe is an essential gene that is involved in several different DNA repair processes. Rad18 (Smc6) is a member of the structural maintenance of chromosomes (SMC) family and, together with its SMC partner Spr18 (Smc5), forms the core of a high-molecular-weight complex. We show her...

journal_title：Molecular and cellular biology

authors： Sergeant J,Taylor E,Palecek J,Fousteri M,Andrews EA,Sweeney S,Shinagawa H,Watts FZ,Lehmann AR

更新日期：2005-01-01 00:00:00

abstract：:The expression of the c-jun proto-oncogene is rapidly induced in response to mitogens acting on a large variety of cell surface receptors. The resulting functional activity of c-Jun proteins appears to be critical for cell proliferation. Recently, we have shown that a large family of G protein-coupled receptors (GPCRs...

journal_title：Molecular and cellular biology

authors： Marinissen MJ,Chiariello M,Pallante M,Gutkind JS

更新日期：1999-06-01 00:00:00